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Changeable aspects of radiation-induced chromosome aberrations during early development of the zygote

Research Project

Project/Area Number 13680615
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionAsahikawa Medical College

Principal Investigator

KAMIGUCHI Yujiroh  Asahikawa Med.Col., Dept.Biol.Sci., Professor, 医学部, 教授 (60091568)

Co-Investigator(Kenkyū-buntansha) TATENO Hiroyuki  Asahikawa Med.Col., Dept.Biol.Sci., Associate Professor, 医学部, 助教授 (80163492)
KUSAKABE Hirokazu  Asahikawa Med.Col., Dept.Biol.Sci., Research Associate, 医学部, 教務職員 (60344579)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsmouse spermatozoa / mouse oocytes / gamma-rays / chromosome aberrations / in-vitro fertilization / early development
Research Abstract

To understand changeable aspects of radiation-induced chromosome aberrations (CAs) during the embryonic development, mouse pie-implantation and post-implantation embryos derived from gamma-irradiated (2,4 Gy) spermatozoa were studied cytogenetically and embryologically.
1.Ahighly successful method for chmmosome preparation of mouse 1-cell to 4-cell embryos was established.
2.A total number of 1,170 zygotes were karyotyped in irradiated and control groups. The zygotes with structural chromosome aberrations (SCAs) showed a clear dose-dependent increase, reaching 36% in the 4 Gy group. No dose-dependent increase of aneuploidy was found. The most predominant type of CA was chromosome breaks/fragments (60%) which was followed by dicentric chromosomes (25%).
3.A total number of 396 2-cell embryos were karyotyped. The incidence of embryos with SCAs was nearly the same as that in 1-cell embryos, but about 90% of them converted into mosaic-type SCA. The incidence of SCA-derived aneuploidy was also significantly high in the irradiated groups.
4.A total number of 537 4-cell embryos were karyotyped. A great majority of the embryos with SCA were of mosaic-type. The incidence of aneuploidy became decreased, suggesting that they converted again into mosaic type.
5.About 50 to 60% of embryos with SCA reached to blastocyst stage, showing no developmental arrest or delay.
6.Chromosome analysis was carried out with 2,240 somatic cells obtained from 85 live embryos (10〜11-day old). None of embryos was found to be chromosomally abnormal. The incidence of post-implantation death was significantly high in the irradiated groups, almost all the dead embryos were being in the state of degenerated conceptus. These suggest that selection of the embryos with CA mainly takes place immediately after implantation.
7.No increase of embryonic death or developmental anomalies was observed in 16〜17-day-old fetuses (survey of 52 pregnant females), supporting the above-mentioned suggestion.

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All 2004 2003 2002 Other

All Journal Article (24 results) Publications (1 results)

  • [Journal Article] Abnormal chromosome migration and chromosome aberrations in mouse oocytes during meiosis II in the presence of topoisomerase II inhibitor ICRF-1932004

    • Author(s)
      Hiroyuki Tateno
    • Journal Title

      Mutation Research 502

      Pages: 1-9

    • NAID

      120003161708

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromosomal integrity of freeze-dried mouse spermatozoa after ^<137>Cs gamma-ray irradiation2004

    • Author(s)
      Hirokazu Kusakabe
    • Journal Title

      Mutation Research 556

      Pages: 163-168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ability to activate oocytes and chromosome integrity of mouse spermatozoa preserved in EGTA Tris-HCl buffered solution supplemented with antioxidants2004

    • Author(s)
      Hirokazu Kusakabe
    • Journal Title

      Theriogenology 62

      Pages: 897-905

    • NAID

      120003132211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (-)-epi-gallocatechin (EGCG)2004

    • Author(s)
      Hirokazu Kusakabe
    • Journal Title

      Mutation Research 564

      Pages: 195-200

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromosome analysis of mouse one-cell androgenones derived from a sperm nucleus exposed to topoisomerase II inhibitors at pre- and post-fertilization stages2004

    • Author(s)
      Hiroyuki Tateno
    • Journal Title

      Mutation Research 556

      Pages: 117-126

    • NAID

      120003132195

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromosomal integrity of freeze-dried mouse spermatozoa after ^<137>Cs gamma-ray irradiation2004

    • Author(s)
      Kusakabe, H. et al.
    • Journal Title

      Mutation Research 556

      Pages: 163-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ability to activate oocytes and chromosome integrity of mouse spermatozoa preserved in EGTA Tris-HCl buffeted solution supplemented with antioxidants2004

    • Author(s)
      Kusakabe, H. et al.
    • Journal Title

      Theriogenology 62

      Pages: 897-905

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (-)-epigallocatechin gallate (EGCG)2004

    • Author(s)
      Kusakabe, H. et al.
    • Journal Title

      Mutation Research 564

      Pages: 195-200

    • NAID

      120003132193

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromosome analysis of mouse one-cell androgenones derived from a sperm nucleus exposed to topoisomerase II inhibitors at pre-and post-fertilization stages2004

    • Author(s)
      Tateno, H. et al.
    • Journal Title

      Mutation Research 556

      Pages: 117-126

    • NAID

      120003132195

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inability of mature oocytes to create functional haploid genomes from somatic cell nuclei2003

    • Author(s)
      Hiroyuki Tateno
    • Journal Title

      Fertility and Sterility 79

      Pages: 216-218

    • NAID

      120003161776

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reproductive semicloning biparental origin. A biologically unsound principle2003

    • Author(s)
      Hiroyuki Tateno
    • Journal Title

      Human Reproduction 18

      Pages: 472-475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Avian chromosomes can be examined by injection of erythrocyte nuclei into mouse oocytes2003

    • Author(s)
      Hiroyuki Tateno
    • Journal Title

      Hereditas 138

      Pages: 18-159

    • NAID

      120003161743

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inability of mature oocytes to create functional haploid genomes from somatic cell nuclei2003

    • Author(s)
      Tateno, H. et al.
    • Journal Title

      Fertility and Sterility 79

      Pages: 216-218

    • NAID

      120003161776

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reproductive semi-cloning biparental origin. A biologically unsound principle2003

    • Author(s)
      Tateno, H. et al.
    • Journal Title

      Human Reproduction 18

      Pages: 472-475

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Avian chromosomes can be examined by injection of erythrocyte nuclei into mouse oocytes2003

    • Author(s)
      Tateno, H. et al.
    • Journal Title

      Hereditas 138

      Pages: 158-159

    • NAID

      120003161743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 被曝した精子や卵子が受精したら2002

    • Author(s)
      上口 勇次郎
    • Journal Title

      Isotope News 583

      Pages: 2-6

    • NAID

      10010432358

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Radiation- and chemical-induced structural chromosome aberrations in human spermatozoa2002

    • Author(s)
      Yujiroh Kamiguchi
    • Journal Title

      Mutation Research 504

      Pages: 183-191

    • NAID

      120003132206

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 精子の形態、運動性と染色体異常2002

    • Author(s)
      立野 裕幸
    • Journal Title

      Medical Science Digest 28

      Pages: 138-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A cytogenetic study of in-vitro matured marine oocytes after ICSI by human sperm2002

    • Author(s)
      Keiko Tsuchiya
    • Journal Title

      Human Reproduction 17

      Pages: 420-425

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] What happens after fertilization of radioactive ray-exposed sperm and oocytes2002

    • Author(s)
      Kamiguchi, Y.
    • Journal Title

      Isotope News 583

      Pages: 2-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Radiation and chemical-induced structural chromosome aberrations in human spermatozoa2002

    • Author(s)
      Kamiguchi, Y. et al.
    • Journal Title

      Mutation Research 504

      Pages: 183-191

    • NAID

      120003132206

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Abnormal chromosome migration and chromosome aberrations in mouse oocytes during meiosis II in the presence of topoisomerase II inhibitor ICRF-1932002

    • Author(s)
      Tateno, H. et al.
    • Journal Title

      Mutation Research 502

      Pages: 1-9

    • NAID

      120003161708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Correlation between the morphology and motility of spermatozoa and the chromosome aberration2002

    • Author(s)
      Tateno, H. et al.
    • Journal Title

      Medical Science Digest 28

      Pages: 138-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A cytogenetic study of in-vitro matured murine oocvtes after ICSI by human sperm2002

    • Author(s)
      Tsuchiya K. et al.
    • Journal Title

      Human Reproduction 17

      Pages: 420-425

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 上口 勇次郎: "被曝した精子や卵子が受精したら"Isotope News. Nov.'02 No.583. 2-6 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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