| Project/Area Number |
13680633
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCE |
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Principal Investigator |
MIURA Takashi TOKYO UNIVERITY OF PHARMACY AND LIFE SCIENCE, PROFESSOR, 生命科学部, 教授 (70013323)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAYA Yoshio THE NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES, CHIEF RESEARCHER, 地域環境グループ, 主任研究員
YOSHIMI Tatuya TOKYO UNIVERITY OF PHARMACY AND LIFE SCIENCE, RESEARCH ASSOCIATE, 生命科学部, 助手 (30277256)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Environmental risk assessment / Chironomus yoshimatsui / gene expression / Midges in sediment |
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| Research Abstract |
In order to develop the methods assessing environmental stress using gene expression of aquatic midges, the stress-induced or insecticide-induced gene fragments were isolated and gene expression was examined in insecticide-sensitive and field strains of Chironomus yoshimatsui
1) Assessment of environmental stress by expression of stress-induced genes
The gene fragment of heat-shock protein, hsc70 was isolated from mRNA of field strain of the midge by RT-PCR reaction using the primer designed from hsp70 base sequences of four species insects and used for Northern blot analysis. In order to assess environmental stresses, RNA isolated from field strains were hybridized with hsc70 cDNA. The expression of this gene was higher in the midges collected in early May
The inducible gene of hsp70 was also isolated using RNA from heat-treated midges by PT-PCR reaction using the primer designed from base sequence of Chironomus tentans and determined the sequence of full length gene. Using the probe specific for inducible gene, the expression of hsp70 was examined in RNA of heat-treated midges and revealed to reach the peak within 1hour and decline 6 hours later. These results show that the isolated hsp70 gene is inducible
2) Assessment of gene expression induced by insecticide-treatment
In order to assess insecticide-stress, RNAs were isolated from insecticide-treated midges and gene fragments increased by insecticide-treatment were separated in differential display method. Four fragments were isolated. The expression of these gene were higher in insecticide-treated midges and in field strains
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