| Project/Area Number |
13680672
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKENAKA Shigeori School of Engineering, Department of Applied Chemistry, Associate Professor, 工学研究院, 助教授 (60188208)
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| Co-Investigator(Kenkyū-buntansha) |
NOJIMA Takahiko School of Engineering, Department of Applied Chemistry, Research Associate, 工学研究院, 助手 (00291930)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | human genome / SNP / naphthalene diimide / ferrocene / intercalator / cyclic intercalator / electrochemical measurement / molecular probe / アントラキノン / 大環状インターカレータ / 一塩基多型(SNP) / 解析プローブ / ハイブリダイゼーション / ヘテロ二重らせん / 融解温度 / ナフタレンジイミド / 遺伝子多型 / ミスマッチ塩基 / X線構造解析 |
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| Research Abstract |
The research on the human genome leads to single nucleotide polymorphism (SNP) analysis eventually at the individual level. The goal of this project was, hence, to develop a cyclic intercalator, CyNDIFc, composed of naphthalene diimide and ferrocene parts as an electrochemical probe for SNP analysis. The integrity of the structure of CyNDIFc newly synthesized was confirmed by X-ray analysis. The effect of CyNDIFc on the mismatched DNA region was evaluated by Tm measurements of a DNA duplex in its presence. The Tm value in the presence of CyNDIFc increased when a mismatched base was introduced in the DNA duplex. Electrochemical measurement was performed by using a DNA-immobilized electrode in order to detect the mismatched base on the DNA duplex. The current from CyNDIFc increased as mismatched base regions increased. These results showed that CyNDIFc is a promising candidate as a new molecular probe for SNP analysis. Further optimization of the CyNDIFc structure will lead to molecular probes with even higher selectivity and sensitivity.
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