| Project/Area Number |
13680701
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Tokyo Metropolitan Institute of Medical Science |
|---|
Principal Investigator |
KASAHARA Kohji Tokyo Metropol Inst Med Sci, 東京都臨床医学総合研究所, 研究員 (60250213)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | glycosphingolipids / raft / GPI-anchor / signal transduction |
|---|
| Research Abstract |
The mechanism of signal transduction by GPI-anchored neural cell adhesion molecules is obscure, because GPI-anchored proteins have no transmembrane domains. Recent studies suggest dynamic clustering of glycosphingolipids (lipid rafts) function as platforms for attachment of GPI-anchored proteins, src-family kinases during signal transduction. In this research project, I pursued clarifying mechanism of signal transduction by GPI-anchored neural cell adhesion molecules.
I previously reported that GPI-anchored neural cell adhesion molecule TAG-1 transduces signal via src-family kinase Lyn in lipid rafts of rat primary cerebellar cultures. In this year, I found that phosphacan, TAG-1 ligand, induces signal transduction in lipis rafts via src-family kinase Lyn and has repulsive effects on rat primary cerebellar cultures.
|
