• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functions of TIMP-3 regulating progression of the colorectal cancers

Research Project

Project/Area Number 13680708
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

YANA Ikuo  Institute of Medical Science, Lecturer, 医科学研究所, 講師 (70332583)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordscolorectal cancer / TIMP-3 / microarray / TIMP-1 / SW480 / SW620 / マイクロアレイ / TIM-3 / MTI-MMP
Research Abstract

In order to understand how colorectal cancers convert into further invasive, expression profile of SW480 cells were compared to that of SW620 which was independently established with 1ymph node dissemination by SW480. About 5000 genes were analysis in each cells using a DNA microarray, selected 72 genes which were expressed over twice and 112 genes which were less than half in SW620, respectively. In a separate assessment by RT-PCR, expression of tissue inhibitors of matrix metalloproteinase were analyzed in each cells. Consistent with the previous reports, TIMP-1 was up- and TIMP-3 was down regulated in SW620. It has been demonstrated that SW620 behaves more invasive than SV480 in vitro or in vivo, further speculated that the gene silencing of TIMP-3 is involved in the phenotype. In our assay, however, both of cells shows low invasiveness similarly in vitro, or even in in vivo model for liver metastasis. To know the reason why both cells keep less-invasive, MT1-MMP, that is thought to be important for tumor invasion and one of the targets of TIMP-3, was evaluated on Western blot with an anti-MT1-MMP Antibody.
Actually the level of the expression was considerably low in both of |he tumor cells. On the other hand, SW620 showed, a rapid growing in vivo more than SW480. Next question would, then, be an address understanad how the proliferation could be related with the gene expression profile including TIMP-1 and -3.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Takamasa Uekita: "Cytoplasmic tail-dependent internalization of membrane-type I marix metalloproteinase is important for its invasion promoting activity"Journal of Cell Biology. 155. 1345-1356 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 梁 幾勇: "癌浸潤におけるマトリックスメタロプロテアーゼの役割"Surgry Frontia. 8. 19-26 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikuo Yana: "MT1-MMP plays pivotal role in cancer dissemination"Clinical and Experimental Metastasis. 19. 209-215 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uekita T, Itoh Y, Yana I, Ohno H, Seiki M.: "Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity"J Cell Biol. 155. 1345-1356 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikuo Yana and Motoharu Seiki: "The role of MMPs in tumor invasion"Surgery Frontia. 8. 19-26 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yana I, Seiki M.: "Clin Exp Metastasis"MT-MMPs play pivotal roles in cancer dissemination. 19. 209-215 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 上北 尚正: "Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion promoting activity"Journal of Cell Biology. 155・7. 1345-1356 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 梁 幾勇: "癌浸潤におけるマトリックスメタロプロテアーゼの役割"Surgery Frontia. 8・2. 19-26 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 梁 幾勇: "MT1-MMP plays pivotal roles in cancer metastasis"Clinical and Experimental Metastasis. 19. 209-215 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 梁 幾勇: "Matrix Metalloproteinases (MMPs) Regulate Fibrin-invasive Activity via MT1-MMP-dependent and -independent Processes"Journal of Experimental Medicine. 295・2. 295-308 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森 英俊: "CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain"EMBO Journal. 21・15. 3949-3959 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 梁 幾勇: "マトリックスメタロプロテアーゼ(MMP)研究の歴史と最先端"日本消化器外科学会雑誌. 100・2. 144-151 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上北尚正: "Cytoplasmic tai-・dependent internalization of membrane-type metalloproteinase is important for its invasion-promoting activity 1matrix"Journal of Cell Biology. 155・7. 1345-1356 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 梁 幾男: "癌浸潤におけるマトリックスメタロプロテアーゼの役割"Surgery Frontia. 8・2. 19-26 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 梁 幾男: "MTI1-MMP plays pivotal roles in cancer metastasis"Clinical and Experimental Metastasis. (In press). (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi