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A study to clarify the role of postsynaptic cells in synaptic plasticity by using double patch clamp method.

Research Project

Project/Area Number 13680735
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

TANIFUJI Takako  Graduate School of Sciences, Research Associate, 大学院・理学系研究科, 助手 (10311648)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordssynapse / synapse formation / CaMKII / patch damp / Drosophila / 神経筋接合部 / シナプス発生 / 標的細胞 / 可塑性 / 逆行性因子 / 神経・筋接合部 / シナプス伝達 / シナプス前細胞
Research Abstract

We have been studying the role of postsynaptic CaMKII during development of synapses at Drosophila neuromuscular junctions by using the system which consists of two muscles innervated by the same neurons. Activity of CaMKII can be manipulated in one of the two muscles or both muscles by GAL4-UAS expression system. We simultaneously recorded the response of those adjacent postsynaptic muscle cells by the double-patch clamp method. This seems to be a unique way to detect the specific effect of postsynaptic changes. We have found that postsynaptic activation of CaMKII promotes coordinated maturation of pre- and postsynaptic sites of newly hatched larvae in a synapse-specific manner. We also showed that its promotive effects were reduced in the older larvae which were about 7 hrs after hatching. On the other hand, synapses on the muscle cell in which CaMKII activity was not manipulated were affected in the older larvae. These results suggest that the action of CaMKII changes during synaptic development and the propagation of synaptic modification from-one synapse to the other appear only after a certain developmental stage of synapses.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kazama, H., Morimoto-Tanifuji, T., Nose A: "Postsynaptic Activation of CaMKII Promotes Coordinated Pre-and Postsynaptic Maturation of Drosophila Neuromuscular Junctions"Neuroscience. 117・3. 615-625 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazama, H., Morimoto-Tanifuji, T., and Nose A.: Neuroscience. 117-3. 615-625 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazama, H., Morimoto Tanifuji, T., Nose, A.: "Postsynaptic Activation of CaMKH Promotes Coordinated Pre and Postsynaptic Maturation of Drosophila Neuromuscular Junctions"Neuroscience. 117・3. 615-625 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takako Morimoto, Tanifuji, Hokto Kazam, Akinao Nose: "Retrograde signaling in the development of synapses : Involvement of CaMKII in the maturation of presunaptic function"Society for Neuroscience Abstracts. 27・2. 2400 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2022-01-20  

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