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Architecture and Principle of Formation of the Baseplate of Bacteriophage T4

Research Project

Project/Area Number 13680736
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTokyo Institute of Technology

Principal Investigator

ARISAKA Fumio  Grad. Sch. Biosci. & Bioeng. Assoc. Professor, 大学院・生命理工学研究科, 助教授 (80133768)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordstail lysozyme / lysozyme / association equilibrium / β-helix / bacteriophage / X-ray crystallography / infection / 感染 / 分子シャペロン / 繊維状蛋白質 / 熱変性 / グアニジン塩酸変性 / 分子集合 / リゾチーム / 収縮性尾部 / 感染機構 / X線結晶解析 / 超遠心分析
Research Abstract

Crystal structure of the tail lysozyme, gp5, which is a structurally essential component of the baseplate and also possesses lysozyme activity has been determined to the resolution of 2.9 A. Gp5 of which the C-terminus had His-Tag was bound to Ni-NTA agarose gel and the column was then used as an affinity chromatograph to isolate separately prepared Seleno-Met substituted gp27 which is another component of the ″hub″of the baseplate for phase determination.
Isolated complex from the Ni-NTA agarose column had the subunit composition of (gp5*)^3(gp5C)^3(gp27)^3, where gp5* is the mature tail lysozyme, gp5C is the C-terminal domain. The overall shape of the complex appears a "torch", where trimer gp27 and the N-terminal domain form the cup, the lysozyme domain forms the rim, and the C-terminal domain assumes the "stem". The three domains of gp5 are connected by two linkers with 44 residues (linker 1) and 49 residues (linker 2). The N-terminal domain possesses a so-called OB motif (OB stands for oligonucleotide/oligosaccharide binding) which is a five-stranded beta-barrel. The lysozyme domain is similar to T4 lysozyme with the rms of 1.1 A. The C-terminal domain forms a long prism with the length of 110 A and the width of 28 A. The N-terminal 46 residues in the C-terminal domain form 5-stranded anti-parallel β-sheets. The three beta sheets, from each subunit, form a short prism. The remaining part forms a triple-stranded β-helix. The cross-section of the β-helix reveals three identical sequence of VXGXXXX from each polypeptide chain with a kink at glycine residue which forms the vertice of each triangle. The β-strands form parallel beta sheets. The β-helix is a very stable structure which is resistant to denaturation by SDS at room temperature.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Fumio ARISAKA: "The Tail Lysozyme Complex of Bacteriophage T4"The Int. J. Biochem. & Cell Biol.. 35. 16-21 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Li ZHAO: "P15 and P3, the Tail Completion Proteins of Bacteriophage T4, Both Form Hexameric Rings"J. Bacteriol.. 185. 693-1700 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Eric MILLER: "The Genome of Bacteriophage T4"Microbiol. & Mol. Biol. Rev.. 67. 86-156 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshiharu SUZUKI: "Cold-adaptation mechanism of mutant enzymes of 3-isopropylmalate dehydrogenase from Thermus thermophilus"Prot. Eng.. 15. 471-476 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shuji KANAMARU: "The Structure of the Bacteriophage T4 Cell-Puncuring Device"Nature. 415. 457-560 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kimie ASANUMA: "Multimerization and collagen binding of vitronectin is modulated by its glycosylation"Intern. Congress Sereies. 1223. 97-101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fumio ARISAKA: "The Tail Lysozyme Complex of Bacteriophage T4"The Int. J. Biochem. & Cell BioL... 35. 16-21 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Li ZHAO: "P15 and P3, the Tail Completion Proteins of Bacteriophage T4, Both Form Hexameric Rings"J. Bacteriol.. 185. 693-1700 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Eric MILLER: "The Genome of Bacteriophage T4"Microbiol. & Mol. Biol. Rev.. 67. 86-156 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshiharu SUZUKI: "Cold-adaptation mechanism of mutant enzymes of 3-isopropylmalate dehydrogenase from Thermus thermophilus"Prot. Eng.. 15. 471-476 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shuji KANAMARU: "The Structure of the Bacteriophage T4 Cell-Puncuring Device"Nature. 415. 457-560 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kimie ASANUMA: "Multimerization and collagen binding of vitronectin is modulated by its glycosylation"Intern. Congress Sereies. 1223. 97-101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shuji KANAMARU: "The Structure of the Bacteriophage T4 Cell-Puncuring Device"Nature. 415. 553-557 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fumio ARISAKA: "The Tail Lysozyme Complex of Bacteriophage T4"The Int.J.Biochem.& Cell Biol.. 35. 16-21 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Li ZHAO: "P15 and P3, the Tail Completion Proteins of Bacteriophage T4, Both Form Hexameric Rings"J.Bacteriol.. 185. 1693-1700 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Eric MILLER: "The Bacteriophage T4 Genome"Microbiol.Mol.Biol.Rev.. 67. 86-156 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 金丸周司: "T4ファージの構造生物学と感染のメカニズム"生化学. 74. 131-135 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 金丸周司: "T4ファージの注射針複合体"生物物理. 43. 21-24 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shuji KANAMARU: "The Structure of the Bacteriophage T4 Cell-Puncuring Device"Nature. 415. 457-560 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshiharu SUZUKI: "Adaptation of a thermophilic enzyme, 3-isopropylmalate dehydrogenase, to low temperatures"Protein Engineering. 14. 85-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 有坂文雄: "T4ファージの自己集合におけるシャペロンの機能"ウイルス. 51・1. 57-61 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kei-ichiro INAMORI: "Phylogenetic Perspectives on the Vertebrate Immune System"Kluwer Academic/Plenum Publishers.. 6 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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