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Calorimetric and dielectrpspectroscopic studies of energy-transfer mechanism of within a protein molecule

Research Project

Project/Area Number 13680744
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKyushu Institute of Technology

Principal Investigator

KODAMA Takao  Kyushu Institute of Technology, Faculty of Computer Science and Systems Engineering, Professor, 情報工学部, 教授 (30034200)

Co-Investigator(Kenkyū-buntansha) KOMATSU Hideyuki  Kyushu Institute of Technology, Faculty of Computer Science and Systems Engineering, Assistant Professor, 情報工学部, 助手 (90253567)
SUZUKI Makoto  Tohoku University, Faculty of Engineering, Professor, 大学院・工学研究科, 教授 (60282109)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsCalorimetry / Dielectric spectroscony / Glucosyltransferase / Domain-domain interaction / Enthalpy / Entropy / Hydroahobic interaction / ΔH / ΔS compensation
Research Abstract

The I-type of glucosyltransferase of Streptococcus sobrinus was used to study the of energy-transfer mechanism of within a protein molecule. Protein samples of whole GTF molecule(GTF), its catalytic domain (GS) and glucanbinding domain (DXB) were subjected to differential scanning calorimetry and dielectric and CD spectroscopies. DSC results indicated the enthalpy change of thermal denaturation of GTF{ΔH(GTF)} <ΔH(GS)+ ΔH(DXB). In addition, the T_m (mid-temperature of thermal denaturatin) of GS fused with DXB (within GTF) was lower than that of un-fused GS, whereas T_m of DXB fused with GS (within GTF) was higher than that of un-fused DXB. These results indicate that fusion of GS and DXB may destabilize the former and stabilize the latter. However, CD-spectroscopy indicated that the fusion did not affect the secondary and higher-order structures of either domains. In accordance with this result, dielectroscopy did not show any significant hydration change accompanying the domain fusion.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] NaziaUmmeSalma, Kabir Syed Rashel, 降旗佳男, 児玉孝雄, 鈴木誠: "Dielectric property of Alkali-Halide salts with hydration shells in water"生物物理. 43・S1. S26 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 片山基輝, 深田はるみ, 小松英幸, 児玉孝雄: "不溶性グルカン合成酵素デキストラン結合の定量的解析"生物物理. 43・S1. S73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] KabirSyed Rashel, 横山慶一, 御橋廣真, 児玉孝雄, 鈴木誠: "Microwave dielectric properties of hydrated F-actin in solution"生物物理. 43・S1. S125 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Siddique Md.Shahjahan Parvez, 横山慶一, 佐々木嶺一, 児玉孝雄, 鈴木誠: "Enhanced fluctuation of actin filament copolymerized with acto-S1dS chimera protein upon ATP hydrolysis"生物物理. 43・S1. S128 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小松英幸, 入佐正幸, 児玉孝雄: "溶液中のラチェットモデル"生物物理. 43・S1. S140 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 鈴木誠, 児玉孝雄, カビルサイドラセル, 御橋廣真: "アクチンフィラメント周りの運動性の高い水の存在"生物物理. 43・S1. S19 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 亀井 敬, 児玉 孝雄, 稲積 広平, 鈴木 誠: "グルコシルトランスフェラーゼにおけるドメイン間相互作用の水和量測定による解析"生物物理. 42・Supplement2. S19 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲積 広平, 児玉 孝雄, 小松 英幸: "不溶性グルカン合成酵素(GTF-I)におけるドメイン間相互作用の熱力学的解析"生物物理. 42・Supplement2. S46 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小松 英幸, 児玉 孝雄: "口腔連鎖球菌由来グルコシルトランスフェラーゼの自発的フォールディング"生物物理. 42・Supplement2. S189 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 稲積 広平, 児玉 孝雄, 深田はるみ: "不溶性グルカン合成酵素(GTF-I)におけるドメイン間相互作用の熱力学的解析"生物物理. 41・Supplement 1. S35 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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