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Study on the regulatory mechanism for immunoglobulin expression through histone acetylation

Research Project

Project/Area Number 13680768
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research Institution宮崎医科大学

Principal Investigator

TAKECHI Shinji  University of Miyazaki, Miyazaki Medical College, Research Associate, 医学部, 助手 (10222100)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Hidehiko  University of Miyazaki, Frontier Science Research Center, Research Associate, フロンティア科学実験総合センター, 助手 (10301384)
NAKAYAMA Tatsuo  University of Miyazaki, Frontier Science Research Center, Professor, フロンティア科学実験総合センター, 教授 (60031712)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordshistone / acetylation / transcriptional control / gene expression / 脱アセチル化酵素
Research Abstract

We have cloned the chicken homolog of OBF-1, chOBF-1, which comprises 256 amino acids, and exhibits only 65% overall identity to the human and mouse OBF-1 proteins. Amino acid sequence alignment revealed the putative Oct-binding sequence, RPYQGVRVKEPVKELL(K/R)RKRG, which is conserved among chicken, mouse and human. chOBF-1 protein was demonstrated to bind chicken Oct-1 protein by the in vitro immunoprecipitation experiment, and chOBF-1 was shown to functionally activate the chicken immunoglobulin (Ig) light chain promoter in the NIH 3T3 cell. Taken together, these data indicate that the Ig gene transcription machinery, including Oct-1 and OBF-1, has been highly conserved in vertebrate evolution.
To investigate the transcriptional function of chHDAC2, we employed the chIgM-L promoter reporter plasmid. We found that chHDAC2 represses activated chIgM-L promoter activity. In transient expression experiments in NIH 3T3 cell, the specific HDAC inhibitor tricostatin A increased transactivation of chIgM-L promoter activity mediated by chicken Oct-1 and OBF-1 proteins. In transient coexpression of the three class I chicken histone deacetylases (chHDACl-3) tested, only chHDAC2 repressed the activated chIgM-L promoter activity. These findings suggest that chHDAC2 might be recruited to the chIgM-L promoter and specifically repress chIgM-L transcription.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Takechi, S., Adachi, M., Nakayama, T.: "Chicken HDAC2 down-regulates IgM light chain gene promoter activity"Biochem.Biophys.Res.Commun.. 299. 263-267 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takechi, S., Adachi, M., Nakayama, T.: "Cloning and characterization of the chick Oct binding factor OBF-1"Biochim.Biophys.Acta. 1577. 466-470 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohi, T., Saito, K., Takechi, S., Nabesima, K., Tashiro, H., Shiomi, K., Sugimoto, S., Akematsu, T, Nakayama, T., Iwaki, M., Matsukura, S.: "Clinical features and neuropathological findings of familial amyotrophiclateral sclerosis with a His46Arg mutation in Cu/Zn superoxide dismutase"J.Neurol.Sci.. 197. 73-78 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Nakayama, T.: "Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity"Mutat.Res.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takechi, S., Adachi, M., Nakayama, T.: "Chicken HDAC2 down-regulates IgM light chain gene promoter activity"Biochem. Biophys. Res. Commun.. 299. 263-267 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takechi, S., Adachi, M., Nakayama, T.: "Cloning and characterization of the chick Oct binding factor OBF-1"Biochim. Biophys. Acta. 1577. 466-470 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohi, T., Saito, K., Takechi, S., Nabesima, K., Tashiro, H., Shiomi, K., Sugimoto, S., Akematsu, T., Nakayama, T., Iwaki, M., Matsukura, S.: "Clinical features and neuropathological findings of familial amyotrophiclateral sclerosis with a His46Arg mutation in Cu/Zn superoxide dismutase"J. Neurol. Sci.. 197. 73-78 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Nakayama, T.: "Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity"Mutat. Res.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Nakayama, T.: "Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity"Mutat.Res.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takechi, S., Adachi, M., Nakayama, T.: "Cloning and characterization of the chick Oct binding factor OBF-1"Biochim. Biophys. Acta. 1577. 466-470 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takechi, S., Adachi, M., Nakayama, T.: "Chicken HDAC2 down-regulates IgM light chain gene promoter activity"Biochem. Biophys. Res. Commun.. 299. 263-267 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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