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Identification and characterization of scaffold porteis in JNK cascades

Research Project

Project/Area Number 13680777
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKanazawa University

Principal Investigator

YOSHIOKA Katsuji  Kanazawa University, Cancer Research Institute, Professor, がん研究所, 教授 (60200937)

Co-Investigator(Kenkyū-buntansha) ITO Michihiko  Kitasato University, School of Science, Associated Professor, 理学部, 講師 (90240994)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsMAP kinase / Signal transduction / mouse embryonic stem cell / Scaffold protein / 細胞内シグナル伝達 / JNK
Research Abstract

Scaffold proteins in MAP kinase(MAPK) signal transduction pathways organize MAPK signaling components into functional MAPK modules, thereby enabling the efficient and specific activation of MAPK pathways. We previously identified two novel JNK MAPK-binding proteins, termed JSAP1 and JNKBP1, which may function as scaffolding proteins in the MAPK pathways. In this research project, we have further analyzed the putative scaffold proteins, and obtained the following results. 1.We have identified family members of JSAP1 and JNKBP1,termed JSAP2 and JNKBP2,respectively. 2.We have analyzed the functional relationship between JSAP1 and ASK MAPKKK that activates JNK and p38 MAPK pathways in response to environmental stresses such as reactive oxygen species. Our data suggest that JSAP1 dynamically participates in signal transduction by forming a phosphorylation-dependent signaling complex in the ASK-JNK signaling module. 3.We established JSAP1-null mouse embryonic stem(ES) cell lines. Neurite outgrowth from the JSAP1-null embryoid bodies was apparently less efficient than from wild type. We also observed altered expression of differentiation markers, especially markers for neuroectoderm during in vitro differentiation of JSAP1-null mutant. These results suggest that JSAP1 may be required for early embryonic neurogenesis. 4.We established stable PC12h cell lines that expressed wild-type JSAP1 and its mutant lacking the JNK-binding domain(JBD). immunocytochemical studies of the cell lines indicated that the mutant JSAP1 was localized to the growth cones of differentiating PC12h cells in a similar manner to wild-type JSAP, suggesting that the proper subcellular localization of JSAP1 along microtubules probably does not require INK signaling.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Akechi, M.: "Expression of JNK cascade scaffold protein JSAP1 in the mouse nervous systems"Neuroscience Research. 39. 391-400 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshioka, K.: "Scaffold proteins in mammalian MAP Kinase Signaling pathways"Recent Research Development in Molecular and Cellular Biology. 3. 317-327 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takino, T.: "A scaffold protein in the c-Tun N-terminal kinase signaling pathway is associated with focal adhesion kinase and tyrosine-phosphorylated"Oncogene. 21. 6488-6497 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuura, H.: "Phosphorylation-dependent scaffolding role of JSAP1/JIP3 in the ASK-JNK signaling pathway"Journal of Biological Chemistry. 277. 40703-40709 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, S.: "Aberrant tau phosphorylation by glycogen synthase kinase-3β and TNK3 induces oligomeric tau fibrils in COS-7 cells"Journal of Biological Chemistry. 277. 42060-42065 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, M.: "Scaffold proteins of the cTun-NH_2-terminal kinase(TNK)signaling pathways in neuronal cells"Recent Research Development in Biochemistry. 4. 271-282 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xu, P.: "In vitro development of mouse embryonic stem cells lacking JNK/stress-activated protein kinase-associated protein 1 (JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis"Journal of Biological Chemistry. 278. 48422-48433 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Enomoto, A.: "Caspase-mediated cleavage of JNK during stress-induced apoptosis"Biochemical and Biophysical Research Communications. 306. 837-842 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takino, T.: "Membrane type1 matrix metalloproteinase regulates collagen-dependent mitogen-activated protein/extracellular signal-regulated kinase activation and cell migration"Cancer Research. 64. 1044-1049 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, S.: "Scaffold protein JSAP1 is transported to growth cones of neurites independent of JNK signaling pathways in PCl2h cells"Gene. 329. 51-60 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuruta, F.: "TNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins"The EMBO Journal. 23. 1889-1899 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akechi, M., Ito, M., Uemura, K., Takamatsu, N., Yamashita, S., Uchiyama, K., Yoshioka, K., Shiba, T.: "Expression of JNK cascade scaffold protein JSAP1 in the mouse nervous systems."Neurosci.Res.. 39. 391-400 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshioka, K., Ito, M.: "Scaffold proteins in mammalian MAP kinase signaling pathways."Recent Res.Devel.Mol.Cell.Biol.. 3. 317-327 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takino, T., Yoshioka, K., Miyamori, H., Yamada, K.M., Sato, H.: "A scaffold protein in the c-Jun N-terminal kinase signaling pathway is associated with focal adhesion kinase and tyrosine-phosphorylated."Oncogene. 21. 6488-6497 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuura, H., Nishitoh, H., Takeda, K., Matsuzawa, A., Amagasa, T., Ito, M., Yoshioka, K., Ichijo, H.: "Phosphorylation-dependent scaffolding role of JSAP1/JIP3 in the ASK1-JNK signaling pathway : A new mode of regulation of the MAP kinase cascade."J.Biol.Chem.. 277. 40703-40709 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, S., Tatebayashi, Y., Akagi, T., Chui, D-H., Miyasaka, T., Planel.E, Tanemura, K., Sun, X., Hashikawa, T., Yoshioka, K., Ishiguro, K., Takashima, A.: "Aberrant tau phosphorylation by glycogen synthase kinase-3β and JNK3 induces oliogomeric tau fibrils in COS-7 cells."J.Biol.Chem.. 277. 42060-42065 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, M., Yoshioka, K.: "Scaffold proteins of the cJun-NH_2-terminal kinase(JNK) signaling pathways in neuronal cells."Recent Res.Devel.Biochem.. 4. 271-282 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Xu, P., Yoshioka, K., Yoshimura, D., Tominaga, Y, Nishioka, T., Ito, M., Nakabeppu, Y.: "In vitro development of mouse embryonic stem cells lacking JNK/stress-activated protein kinase-associated protein(JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis."J.Biol.Chem.. 278. 48422-48433 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Enomoto A, Suzuki N, Morita A, Ito M, Liu CQ, Matsumoto Y, Yoshioka K, Shiba T, Hosoi Y.: "Caspase-mediated cleavage of JNK during stress-induced apoptosis."Biochem.Biophys.Res.Commun.. 306. 837-842 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takino, T., Miyamori, H., Watanabe, Y., Yoshioka, K., Seiki, M., Sato, H.: "Membrane-type 1 matrix metalloproteinase regulates collagen-dependent mitogen-activated protein/extracellular signal-regulated kinase activation and cell migration."Cancer Res.. 64. 1044-1049 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, S., Ito, M., Ito, T., Yoshioka, K.: "Scaffold protein JSAP1 is transported to growth cones of neurites independent of JNK signaling pathways in PC12h cells."Gene. 329. 51-60 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsuruta, Fuminori, Sunayama, J., Mori, Y., Hattori, S., Shimizu, S., Tsujimoto, Y., Yoshioka, K., Masuyama, N., Gotoh, Y.: "JNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins."EMBO J.. 23. 1889-1899 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinji Sato: "Scaffold protein JSAP1 tansported to growth cones of neurites independent of JNK signaling pathways in PC12h cells"Gene. 329. 51-60 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahisa Takino: "Membrane-type1 matrix metalloproteinase regulates collagen-dependent mitogen-activated protein/extracellular signal-regulated kinase activation and cell migration"Cancer Research. 64・3. 1044-1049 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ping Xu: "In vitro development of mouse embryonic stem cells lacking JNK/stress-activated protein kinase-associated protein 1 (JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis"Journal of Biological Chemistry. 278・48. 48422-48433 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Atsushi Enomoto: "Caspase-mediated cleavage of JNK during stress-induced apoptosis"Biochemical and Biophysical Research Communications. 306・4. 837-842 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Michihiko Ito: "Scaffold proteins of the cJun-NH_2-terminal kinase (JNK) signaling pathways in neuronal cells"Recent Research Developments in Molecular & Cellular Biology. 4. 271-282 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahisa Takino: "A scaffold protein in the c-Jun N-terminal kinase is associated with focal adhesion kinase and tyrosine-phosphorylated"Oncogene. 24・42. 6488-6497 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroshi Matsuura: "Phosphorylation-dependent Scaffolding Role of JSAP1/JIP3 in the ASK1-JNK Signaling Pathway"Journal of Biological Chemistry. 277・43. 40703-40709 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinji Sato: "Aberrant Tau phosphorylation by glycogen synthase kinase-3β and JNK3 induces oligomeric tau fibrils in COS-7 cells"Journal of Biological Chemistry. 277・44. 42060-42065 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Katsuji Yoshioka: "Scaffold proteins in mammalian MAP kinase signaling pathways"Recent Research Developments in Molecular & Cellular Biology. 3. 317-327 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizuho Akechi: "Expression of JNK cascade scaffold protein JSAP1 in the mouse nervous system"Neuroscience Research. 39・4. 391-400 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shuang-Lin Xiang: "The J domain of Tpr2 regulates its interaction with the proapoptotic and cell cycle checkpoint protein, Rad9"Biochemical and Biophysical Research Communications. 287・4. 932-940 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katsuji Yoshioka: "Scaffold proteins in mammalian MAP kinase signaling pathways"Research Signpost (in press). (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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