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Identification and characterization of a novel organelle gathered around centrosomes in a microtubule-dependent manner

Research Project

Project/Area Number 13680794
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionNational Institute of Genetics

Principal Investigator

SHIINA Nobuyuki  National Institute of Genetics, Structural Biology Center, Assistant Professor, 構造遺伝学研究センター, 助手 (30332175)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsmRNA / Transport / Local translation / Synaptic plasticity / Staufen / FMRP / CaM kinase II / BDNF / p105 / 中心体 / mRNA輸送体 / 樹状突起 / 微小管
Research Abstract

mRNA transport and subsequent local translation of the mRNAs in neuronal dendrites are widely believed to be essential for local regulation of synaptic efficacy. The dendritic mRNAs are transported in densely packed granules containing the mRNAs and ribosomes, called RNA granules.
RNG105 (RNA granule protein 105) is a novel protein we originally identified as a centrosomes- and microtubule-associated protein. Expression of RNG105 protein in cultured cells induces formation of cytoplasmic granules containing ribosomes and mRNAs. RNG105 was expressed highly in neuronal dendrites. These characteristics of RNG105 protein suggested that the RNG105-induced granules may equivalent to RNA granules in neurons. Immunostaining of rat hippocampal slices showed that RNG105 was associated with granules containing ribosomes and mRNAs in neuronal dendrites. Staufen, a protein for mRNA transport, and FMRP, a protein for translational regulation, were identified as the components of the granule. Microtubule- and actin-dependent motors, kinesin and myosin, were also the components of the granule. Furthermore, mRNAs associated with the granule were identified. Many of the mRNAs, such as CaM kinase IIα, CREB and BDNF mRNAs, were the key molecules for long-term synaptic plasticity. These lines of evidence indicated that RNG105 is a novel protein associated with mRNA-transporting granules which may be responsible for synaptic plasticity in neuronal dendrites.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (2 results)

All 2005

All Journal Article (2 results)

  • [Journal Article] A novel RNA-binding protein in neuronal RNA granules, regulatory machinery for local translation2005

    • Author(s)
      Shiina N., Shinkura, K., Tokunaga, M.
    • Journal Title

      The Journal of Neuroscience 印刷中

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A novel RNA-binding protein in neuronal RNA granules, regulatory machinery for local translation2005

    • Author(s)
      Shiina.N., Shinkura, K., Tokunaga, M.
    • Journal Title

      The Journal of Neuroscience (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary

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Published: 2001-04-01   Modified: 2016-04-21  

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