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Establishment of Culture System Capable of Inducing Neural Crest Cells from ES Cells

Research Project

Project/Area Number 13680808
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionGifu University

Principal Investigator

KUNISADA Takahiro  Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (30205108)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsES cell / in vitro differentiation / neural crest cell / melanocyte / endothelin 3 / retinoic acid / 神経冠細胞 / 細胞分化
Research Abstract

Mouse embryonic stem (ES) cells are totipotent, primitive, ectodermal-like cells derived from inner cell mass of 3,5 day blastocysts. As an in vitro model for melanocyte differentiation, we have developed the culture system to induce melanocytes from totipotent ES cell line. When undifferentiated ES cells were co-cultured with a bone marrow-derived stromal cell line, a very small but significant number of melanocytes were reproducibly generated. This process was greatly enhanced by addition of dexamethasone to the culture and strictly depended on the c-Kit signaling and endothelin 3 signaling. In this study, we found that caudalizing factor retinoic acid enhanced the efficiency of melanocyte induction in this culture. The early precursors for melanocytes, which proliferate clonally after several passages, were efficiently induced in the culture and their self-renew was stimulated by the addition of endothelin 3 and also by retinoic acid. These results suggests that neural crest cells are induced in this culture system upon corresponding in vivo development.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Hemmi, H., Okuyama, H., Kunisada, T., 他5名: "Temporal and spatial localization of osteoclasts in colonies from embryonic stem cells"Biochem. Biophys. Res. Commun.. 280. 526-534 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hemmi, H., Yoshino, M., Kunisada, T., 他14名: "Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-b1-dependent cells"Int, Immunol.. 13. 695-704 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang, K., Gan, L., Kunisada, T., 他3名: "Characterization of the Japanese pufferfish (Takifugu rubripes) T-cell receptor alpha locus reveals a unique genomic organization"Immunogenet.. 53. 31-42 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamane, T., Kunisada, T., Tsukamoto, H., 他4名: "Wnt signaling regulates hemopoiesis through stromal cells"J. Immunol.. 167. 765-772 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kunisada, T., Yamazaki, H., Hayashi, S.I.: "Ligands for receptor tyrosine kinases expressed in the skin as environmental factors for melanocyte development"J. Invest. Dermatol.. 6. 6-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida H, Kunisada T, Grimm T, 他3名: "Melanocyte migration and survival controlled by SCF/c-kit expression"J Investig Dermatol.. 6. 1-5 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamane, T., Hayashi, S.I., Kunisada, T.: "Embryonic stem cells as a model to study melanocyte lineage"Methods Mol. Biol.. 185. 261-268 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamane, T,.Kunisada, T., Hayashi, S.I.: "Embryonic stem cells as a model to study osteoclast lineage"Methods Mol. Biol.. 2185. 97-106 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hemmi, H., Okuyama, H., Yamane, T., Nishikawa, S-I., Nakano, T., Yamazaki, H., Kunisada, T. and Hayashi S-I.: "Temporal and spatial localization of osteoclasts in colonies from embryonic stem cells"Biochem. Biophys. Res. Commun.. 280. 526-534 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hemmi, H., Yoshino, M., Yamazaki, H., Naito, M., Iyoda, T., Omatsu, Y., Shimoyama, S., Letterio, J.J., Nakabayashi, T., Tagaya, H., Yamane, T., Ogawa, M., Nishikawa, S.I., Ryoke, K., Inaba, K., Hayashi, S.I. And Kunisada, T: "Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-b1-dependent cells"Int. Immunol.. 13. 695-704 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang, K., Gan, L., Kunisada, T., Lee, I., Yamagishi, H. and Hood, L.: "Characterization of the Japanese pufferfish (Takifugu rubripes) T-cell receptor alpha locus reveals a unique genomic organization"Immunogenet. 53. 31-42 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamane, T., Kunisada, T., Tsukamoto, H., Yamazaki, H., Niwa, H., Takada, S. and Hayashi, S-I.: "Wnt signaling regulates hemopoiesis through stromal cells."J. Immunol. 167. 765-772 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kunisada, T., Yamazaki, H., and Hayashi, S.I.: "Ligands for receptor tyrosine kinases expressed in the skin as environmental factors for melanocyte development"J Invest Dermatol. 6. 6-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshida H, Kunisada T, Grimm T, Nishimura EK, Nishioka E, Nishikawa SI: "Melanocyte migration and survival controlled by SCF/c-kit expression"J Invest Dermatol. 6. 1-5 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamane, T., Hayashi, S.I. and Kunisada, T.: "Embryonic stem cells as a model to study melanocyte lineage"Methods Mol. Biol.. 185. 261-268 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamane, T., Kunisada, T. and Hayashi, S.I.: "Embryonic stem cells as a model to study osteoclast lineage"Methods Mol. Biol.. 2185. 97-106 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamane, T., Hayashi, S.I., Kunisada, T.: "Embryonie stem cells as a model to study melanocyte lineage"Methods Mol. Biol.. 185. 261-268 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamane, T., Kunisada, T., Hayashi, S.I.: "Embryonic stem cells as a model to study osteoclast lineage"Methods Mol. Biol.. 2185. 97-106 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshino M, Yamazaki H, Yoshida H, Niida S, Nishikawa S, Ryoke K, Kunisada T, Hayashi S.: "Reduction of osteoclasts in a critical embryonic period is essential for inhibition of mouse tooth eruption"Bone Miner Res.. 18. 108-116 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hemmi, H. 他: "Temporal and spatial localization of osteoclasts in colonies from embryonic stem cells"Biochem.Biophys.Res.Commun.. 280. 526-534 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hemmi, H. 他: "Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-bl-dependent cells"Int.Immunol.. 13. 695-704 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wang, K. 他: "Characterization of the Japanese pufferfish(Takifugu rubripes)T-cell receptor alpha locus reveals a unique genomic organization"Immunogenet. 53. 31-42 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamane, T. 他: "Wnt signaling regulates hemopoiesis through stromal cells"J.Immunol.. 167. 765-772 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kunisada, T. 他: "Ligands for receptor tyrosine kinases expressed in the skin as environmental factors for melanocyte development"J.Invest.Dermatol.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshino, M. 他: "Reduction of osteoclasts in the critical embryonic period is esgential for inhibition of mouse tooth eruption"Dev.Dynamics. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamane, T. 他: "Embryonic Stem Cells : Methods and Protocols"Ed.K.Turksen,The Humana Press Inc.,Totowa,NJ.. (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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