Study of generation of Alzheimer's amyloid β peptide

• Project/Area Number: 13680814
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Tohoku University
• Principal Investigator: RYONG-WOON Shin Tohoku University, Graduate School of Medicine ,Lecture, 大学院・医学系研究科, 講師 (40271910)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Alzheimer's disease / amyloid β / αcleavage / βcleavage / アミロイド前駆体蛋白 / αsecretase / βsecretase / γsecretase

Research Abstract

Processing of the amyloid precursor protein (APP) includes αand βcleavage pathways. The proteolytic product of the APP, Aβ is generated through initial β cleavage and subsequent γ cleavage pathway. Aβ is considered central in the pathogenesis of Alzheimer's disease, and therefore clarification of the APP processing is an important issue. In our previous study performed in 2001, we demonstrated that the possibility is quite low that APP undergoes processing consisting of γ cleavage prior to α/β cleavage. Namely APP is subject uniquely to the processing consisting of initial α/β cleavage and subsequent γ cleavage pathway. Based on these results, we studied the eubcellular compartments for α and β cleavages. First APP molecule was modified by addition of the Retargeting motif, which restricts the molecule to be expressed in ER without sorting to afterward compartments. This mutant APP was found to give α and β cleavages. Second APP processing in ER was reconstructed using brefeldin A, an agent that destructs Golgi apparatus and thereby inhibits sorting out from ER of expressed proteins. Under this condition APP was found to show α but not β cleavage. Thus APP expressed restrictedly in ER shows a cleavage. The cell surface has been identified as the subcellular site for α cleavage to occur. Here ER was identified another subcellular site for α cleavage.

Research Products

• [Publications] Yamamoto A, Shin R-W, Hasegawa K, Naiki H, Yoshimasu Y, Kitamoto T: "Iron (III) induces aggregation of hyperphosphorylated tau and its reduction to iron (II) reverses the aggregation : implications in the formation of neurofibrillary tangles of Alzheimer's disease"J Neurochem. 82. 1137-1147 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kitamoto T, Mohri S, Ironside JW, Miyoshi I, Tanaka T, Kitamoto N, Itohara S, Kasai N, Katuski M, Higuchi J, Muramoto T, Shin R-W: "Follicular dentritic cell of the knock-in mouse provides a new bioassay for human prions"Biochem Biophys Res Com. 294. 280-286 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shin R-W, Kruck TPA, Murayama H, Kitamoto T: "A novel trivalent cation chelator Feralex dissociates binding of aluminum and iron associated with hyperphosphorylated tau of Alzheimer's disease"Brain Res. 961. 139-146 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kuazi DA, Kito K, Abe Y, Shin R-W, Kamitani T, Ueda N.: "NEDD8 involvement in the ubiquitinated inclusion bodies"J Pathol. 199. 259-266 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamamoto A, Shin R-W, Hasegawa K, Naiki H, YosMmasu Y, Kitamoto T: "Iron (III) induces aggregation of hyperphosphorylated tau and its reduction to iron (II) reverses the aggregation: implications in the formation of neurofibrillary tangles of Alzheimer's disease"J Neurochem. 82. 1137-1147 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kitamoto T, Mohri S, Ironside JW, Miyoshi I, Tanaka T, Kitamoto N, Itohara S, Kasai N, Katuski M, Higuchi J, Muramoto T, Shin RW: "Follicular dentritic cell of the knock-in mouse provides a new bioassay for human prions"Biochem BiophysRes Com. 294. 280-286 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shin R-W, Kruek TPA, Murayama H, Kitamoto T: "A novel trivalent cation chelator Feralex dissociates binding of aluminum and iron associated with hyperphosphorylated tau of Alzheimer's disease"Brain Res. 961. 139-146 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kuazi DA, Kito K, Abe Y, Shin R-W, Kamitani T, Ueda N: "NEDD8 involvement in the ubiquitinated inclusion bodies"J Pathol. 199. 259-266 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamamoto A., Shin R.-W., Hasegawa K., Naiki H., Yoshimasu Y., Kitamoto T.: "Iron (III) induces aggregation of hyperphosphorylated tau and its reduction to iron (II) reverses the aggregation : implications in the formation of neurofibrillary tangles of Alzheimer's disease"J.Neurochem.. 82. 1137-1147 (2002)
• [Publications] Kitamoto T., Mohri S., Ironside J.W., Miyoshi I., Tanaka T., Kitamoto N., Itohara S., Kasai N., Katuski M., Higuchi J., Muramoto T., Shin R.-W.: "Follicular dentritic cell of the knock-in mouse provides a new bioassay for human prions"Biochem.Biophys.Res.Com.. 294. 280-286 (2002)
• [Publications] Shin R.-W., Kruck.T.P.A., Murayama H., Kitamoto T.: "A novel trivalent cation chelator Feralex dissociates binding of aluminum and iron associated with hyperphosphorylated tau of Alzheimer's disease"Brain Res.. 961. 139-146 (2003)
• [Publications] Kuazi D.A., Kito K., Abe Y., Shin R.-W., Kamitani T., Ueda N.: "NEDD8 involvement in the ubiquitinated inclusion bodies"J.Pathol.. 199. 259-266 (2003)
• [Publications] Shin R-W: "Aluminum, tau and neurofibrillary degeneration"Aluminum and Alzheimer's disease : The science that describes the link (Exley C, ed),. New York : Elsevier Science. 411-420 (2001)