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Generation and Functional Analysis for GPI-anchored neural adhesion molecule NB-2 gene knockout mice.

Research Project

Project/Area Number 13680858
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY

Principal Investigator

TAKEDA Yasuo  Dept. Cell Recognition Tokyo Metropol. Inst. Gerontol. Senior Researcher, 東京都老人総合研究所・細胞認識研究グループ, 主任研究員 (60245462)

Co-Investigator(Kenkyū-buntansha) WATANABE Kazutada  Dept. BioEngeering Nagaoka Univ. of Sci. & Tech. Professor, 生物系, 教授 (70114717)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCell Adehesion Molecule / GPI-Anchored Protein / NB-2 / Auditory System / Knockout Mice
Research Abstract

NB-2, a member of the contactin subgroup in the immunoglobulin superfamily, is restrictively expressed in the nervous system, reaching the maximum level at postnatal 3 weeks. NB-2 displays neurite outgrowth-promoting activity in vitro. To assess its function in the nervous system, we generated mutant mice ablating a part of NB-2 gene by replacing with the tau-LacZ gene. The general appearance of NB-2 deficient mice and the gross anatomical features were normal. The LacZ expression patterns in heterozygous mice revealed that NB-2 is preferentially expressed in the central auditory pathways. In the audiogenic seizure susceptibility (AGS) test, NB-2 mutant mice exhibited lower incidence of wild running, but higher rate of mortality than the wild-type littermates. c-Fos expression demonstrated that neural activity induced by the AGS in the NB-2 deficient mice was prominently attenuated in the both dorsal and external cortexes of inferior colliculus where enhanced neural activity is observed in the wild-type mice. Pure-tone stimulation after priming, NB-2 deficient mice exhibited a diffusive and low level of c-Fos expression in the central nucleus of inferior colliculus in comparison with a strong and a tonotopic banding expression of c-Fos in the wild-type littermates. Taken together, these results suggest that lack of NB-2 impairs the neuronal activity in the auditory system.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ando, S., Takeda, Y., Nakamura, H.et al.: "An animal model of demetia induced by entorhinal synaptic damade, and partial restoration of cognitive deficits by BDNF and carnitine"Journal of Neuroscience Research. 70. 519-527 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeda, Y., Akasaka, K., Watanabe, K.et al.: "Impaired motor coordination in mice lacking neural recognition molecule NB-3 of the contactin/F3 subgroup"Journal of Neurobiology. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Li, H., takeda, Y., Watanabe, K., et al.: "Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2"European Journal on Neuroscience. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakamoto, T., Takeda, Y., Watabe, K., et al.: "Adenoviral gene transfer of GDNF, BDNF and TGF-β2, but not CNTF, Cardiotrophin-1, protects injured adult motoneurons after facial nerve avulsion"Journal of Neuroscience Research. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ando, S., Takeda, Y., Nakamura, H. et al.: "An animal model of dementia induced by entorhinal synaptic damade, and partial restoration of cognitive deficits bv BDNF"Journal of Neuroscience Research. 70. 519-527 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeda, Y., Akasaka, K., Watanabe, K. et al.: "Impaired motor coordination in mice lacking neural recognition molecule NB-3 of the contactin/F3 subgroup"Journal of Neurobiology. In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Li. H., Takeda, Y., Watanabe, K. et al.: "Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2"European Journal of Neuroscience. In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakamoto, T., Takeda, Y., Watabe, K. el al.: "Adenoviral gene transfer of GDNF, BDNF and TGF-β2, but not CNTF, Cardiotrophin- 1 , protects injured adult motoneurons"Jounal of Neuroscience Research. In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ando, S., Takeda, Y., Nakamura, H.et al.: "An animal model of dementia induced by entorhinal synaptic damade, and partial restoration of cognitive deficits by BDNF---"Journal of Neuroscience Research. 70. 519-527 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeda, Y., Akasaka, K., Watanabe, K.et al.: "Impaired motor coordination in mice lacking neural recognition molecule NB-3 of the contactin/F3 subgroup"Journal of Neurobiology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Li, H., Takeda, Y., Watanabe, K.et al.: "Aberrant responses to acoustic stimuli in mice deficient for neural recognition molecule NB-2"European Journal of Neuroscience. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakamoto, T., Takeda, Y., Watabe, K.et al.: "Adenoviral gene transfer of GDNF, BDNF and TGF-β2, but not CNTF, Cardiotrophin-1, protects injured adult motoneurons----"Journal of Neuroscience Research. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukamauchi, F., Takeda, Y., Watanabe, K.et al.: "TAG-1-deficient mice have marked elevation of adenosine A1 receptors in the hippocampus."Biochem. Biophys. Res. Commun.. 281. 220-226 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogawa, J., Takeda, Y., Watanabe, K.et al.: "Neural recognition molecule NB-2 of the contactin/F3 subgroup in rats : specificity in neurite outgrowth-promoting activity and ---"J Neurosci Res.. 65. 100-110 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeda, Y., Notsu, T., Uyemura, K.et al.: "Functional analysis for peripheral myalin protein PASII/PMP22: Is it a member of claudin superfamily?"Neurochem. Res.. 26. 599-607 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeda, Y., Murakami, Y., Uyemura, K.et al.: "The roles of cell adhesion molecules on the formation of peripheral I-u myelin."Keio J. Medicine. 50. 240-248 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagata, S., Saito, R., Watanabe, K.et al.: "Multiple variants of receptor-type protein tyrosine phosphatase b are expressed in the central nervous system of Xenopus."Gene. 262. 81-88 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyata, S., Watanabe, K., Hatton, I.G et al.: "Plasticity of neurophypophysial terminals with increased hormonal release during dehydration : Ultrastructural and biochemical -----"J. Comp. Neurol.. 434. 413-427 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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