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Imaging and Intracellular Analysis of Synaptic Plasticity in Spinal Dorsal Horn

Research Project

Project/Area Number 13680866
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionFukui University

Principal Investigator

MURASE Kazuyuki  Faculty of Engineering, professor, 工学部, 教授 (40174289)

Co-Investigator(Kenkyū-buntansha) ASAI Tatsuya  Faculty of Engineering, professor, 工学部, 講師 (60291374)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPain / Optical imaging / Spinal dorsal horn / Presynaptic modulation / スライスパッチ
Research Abstract

1. Imaging of voltage response of marginal cell in the lamina I of the dorsal horn retrogradely stained with voltage-sensitive dye.
Projection neurons in the lamina I were stained by voltage-sensitive dye that was injected in the thalamus or brain stem. After one week of the dye injection, slices of the spinal cord were made and the stained cells were recorded by an optical imaging method. The electrical activities evoked by dorsal root stimulation was successfully recorded optically with high resolution in both time and space. The action potentials and the after potentials were clearly observed. We are currently investigating the underlying mechanism of de-potentiation of LTP with this preparation.
2. Modulation of dorsal horn plasticity induction by corticotropin releasing factor (CRF)
We have searched substances that regulate induction of dorsal plasticity, which takes an important role in the induction of abnormal pain. We have found that CRF biphasically regulates the induction of robust changes in neuronal excitation in the superficial dorsal horn by optical imaging.
3. Optical analysis of changes in extracellular space : Possible role for non-synaptic transmission.
Intrinsic optical signals (IOS) in slice has been suggested to reflect cellular volume or extracellular space. We have revealed that cotransporters and water channels play the major role for the IOS induction in the superficial dorsal horn. We are currently investigating on their modulators and the changes in electrical activities associated with the volume changes by optical imaging.
4. Optical analysis of presynaptic modulation at the primary afferent terminals.
By optical imaging, we were able to record presynaptic events, and we investigated on the modulators. We analysed the presynaptic actions of halothane and caffeine as well as vanilloid receptor agoninsts.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Kusudo K., Asai T., Ikeda H., Takenoshita M., Murase K.: "Inhibitory effect of caffeine on C-fibre-evoked excitation in the rat spinal dorsal horn recorded under Ca2+-free condition : an interaction with halothane"Neuroscience Letters. 336(1). 17-20 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Aasai T., Kusudo K., Ikeda H., Murase K.: "Intrinsic optical signals in the dorsal horn of rat spinal cord slices elicited by brief repetitive stimulation"European Journal of Neuroscience. 15. 1737-1746 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T Asai, K Kusudo, H Ikeda, M Takenoshita, K Murase: "Effect of halothane on neuronal excitation in the superficial dorsal horn of rat spinal cord slices : Evidence for a presynaptic action"European Journal of Neuroscience. 15. 1278-1290 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Seong Kyu Han, Wonee Chong, Long Hua Li, In Se Lee, Kazuyuki Murase, Pan Dong Ryu: "Noradrenaline excites and inhibits GABAergic transmission in parvocellulr neurons of the rat hypothalamic paraventricular nucleus"Journal of Neurophysiology. 87(5). 2287-2296 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Ikeda, K.Kusudo, PD Ryu, K.Murase: "Modulation of Neuronal Plasticity by Corticotropin-Releasing Factor in the Substantia Gelatinosa of Rat Spinal Cord Slices"Pain. (Accepted). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 赤池紀扶 他編: "[第12章5節]脊髄後角膠様質における神経興奮可塑性のイメージング:メタ可塑性と痛みの調節(村瀬、池田 著)"脳機能の解明:生命科学の主潮流(ガイア出版). 483-492 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Ikeda, K.Kusudo, PD.Ryu, K.Murase: "(2003) Modulation of Neuronal Plasticity by Corticotropin-Releasing Factor in the Substantia Gelatinosa of Rat Spinal Cord Slices"Pain (2003, Accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T Asai, K Kusudo, H Ikeda, K Murase: "(2002) Intrinsic optical signals in the dorsal horn of rat spinal cord slices elicited by brief repetitive stimulation"European Journal of Neuroscience. 15. 1737-1746 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K Kusudo, T Asai, H Ikeda, M Takenoshita, K Murase: "(2003) Inhibitory effect of caffeine on C-fiber-evoked excitation in the rat spinal dorsal horn recorded under Ca^<2+>-free condition : an interaction with halothane"Neuroscience Letters. 336(1). 17-20 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T Asai, K Kusudo, H Ikeda, M Takenoshita, K Murase: "(2002) Effect of halothane on neuronal excitation in the superficial dorsal horn of rat spinal cord slices : Evidence for a presynaptic action"European Journal of Neuroscience. 15. 1278-1290 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Seong Kyu Han, Pan Dong Ryu: "(2002) Noradrenaline excites and inhibits GABAergic transmission in parvocellulr neurons of the rat hypothalamic paraventricular nucleus"Journal of Neurophysiology. 87(5). 2287-2296 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Kusudo, T.Asai 1, H.Ikeda 1, K.Murase: "(2003) Effect of capsaicin on C-fiber evoked excitation in the substantia gelatinosa of rat spinal cord slices revealed by the optical imaging technique"The 70th Annual Meeting of the Physiological Society of Japan. Abstract 2P192 (2003.3.25).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Asai, K.Kusudo, H.Ikeda, K.Murase: "(2003) The activity of cation-chloride cotransporters in the substantia gelatinosa of rat spinal cord slices measured by optical imaging"The 70th Annual Meeting of the Physiological Society of Japan. Abstract 3P033 (2003.3.26).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Asai, K.Kusudo, K.Murase: "(2002) A new form of signal transmission : Nonsynaptic volume transmission in the central nervous system"Proceedings of the 3rd Int Conf on Human and Artificial Intelligence Systems (HART2002). Fukui, Japan, Dec 6-7, 2002. 212-214

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Murase: "(2002) Plasticity of optically recorded neuronal excitation in the substantia gelatinosa"Invited Lecture, The International Symposium on Plasticity of Pain System (ISPPS 2002). Fukuoka, Japan (2002.5).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Murase: "(2002) Optical imaging of afferent induced neuronal excitation in the rat spinal dorsal horn in slices : Neuronal plasticity and slow responses"Invited Lecture, The Symposium on, Neurobiology of Pain, The 3rd Asian Pacific International Congress of Anatomists (APICA 2002). Hamamatsu, Japan (2002.3).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Asai, K.Kusudo, M.Takenoshita, K.Murase: "(2001) Inhibitory effect of halothane on the presynaptic terminal activity in the dorsal horn of rat spinal cord slices revealed by optical imaging technique"The 31st Annual Meeting of the Society for Neuroscience Abstracts. Vol.27, Abstract No.711.1 (2001.11).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Asai, T.Sashima, K.Murase: "(2001) An optical measurement of cation-chloride cotransport activity in rat spinal cord slices"Cell & Molecular Biology of Membrane Transport Systems and Disorders (CMBMTSD) Conference. Abstract pp20 (2001.9).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Asai, T.Sashima, K.Murase: "(2001) A mercury-sensitive water transport in the rat spinal cord revealed by an optical imaging technique"XXXIV International Congress of Physiological Sciences. Abstract pp1577 (2001.8).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai T., Kusudo K., Ikeda H., Murase K.: "Intrinsic optical signals in the dorsal horn of rat spinal cord slices elicited by brief repetitive stimulation"European Journal of Neuroscience. 15. 1737-1746 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusudo K., Asai T., Ikeda H., Takenoshita M., Murase K.: "Inhibitory effect of caffeine on C-fibre-evoked excitation in the rat spinal dorsal horn recorded under Ca2+-free condition : an interaction with halothane"Neuroscience Letters. 336(1). 17-20 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asai T., Kusudo K., Murase K.: "A new form of signal transmission : Nonsynaptic volume transmission in the central nervous system"Proceedings of the 3rd International Conference on Human and Artificial Intelligence Systems. 212-214 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asai T., Kusudo K., Murase K.: "Delayed optical signals recorded from the dorsal horn of rat spinal cord slices and their dependence on the extracellular Ca^<2+> concentration"Neuroscience Research, Supplement. 25. S110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Murase K.: "Optical imaging of afferent induced neuronal excitation in the rat spinal dorsal horn in slices : Neuronal plasticity slow responses"Kaibougaku Zasshi. 77(Suppliment). APICA17 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 赤池紀扶他編: "脳機能の解明:生命科学の主潮流[第12章5節]脊髄後角膠様質における神経興奮可塑性のイメージング:メタ可塑性と痛みの調節(村瀬、池田著)"ガイア出版. 483-492 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asai, T: "Effect of halothane on neuronal excitation in the superficial dorsal horn of rat spinal cord slices : Evidence for a presynaptic action"European Journal of Neuroscience. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seong Kyu Han: "Noradrenaline excites and inhibits GABAergic transmission in parvocellulr neurons of the rat hypothalamic paraventricular nucleus"Journal of Neurophysiology. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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