• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Descending projections of GABA and 5-HT neurons from medullary raphe nuclei to the phrenic nuleus.

Research Project

Project/Area Number 13680901
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionSapporo Medical University

Principal Investigator

AOKI Mamoru  Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (50001871)

Co-Investigator(Kenkyū-buntansha) MATSUYAMA Kiyoji  Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (40209664)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsRaphe magunus / Raphe pallidus / GABA / 5-HT / Respiratory movement / Pherenic muscles / Cat / 上部頸髄呼吸性ニューロン
Research Abstract

Projections of γ-aminobutyric acid(GABA) and serotonin(5-HT) neurons in the medullary raphe nuclei to the phrenic motor nucleus was studied using a combined method of retrograde WGA-HRP(wheat germ agglutinin-conjugated horseradish peroxidase) labeling and anti-GAD(glutamic acid decarboxylase) or anti-5-HT immunostaining. Localized injection of WGA-HRP was made into the phrenic motor nucleus in C_5-C_6 segments in six cats. After a post-injection survival period of 72 hrs, the colchicine treated animals were perfused and serial transverse sections(50μm thick) of the brainstem were made. The sections were first processed for HRP reaction with TMB-Co-DAB method and further processed for anti-GAD or anti-5-HT immunostaining with anti-GAD or anti-5-HT primary antibody. The sections were further incubated in FITC conjugated secondary antibody.
GAD immunopositive and HRP double-labeled(GAD-HRP double-labeled) neurons were observed in several respiratory related structures including the raphe magnus(RM), the ventromedial area of the retrofacial nucleus(Bot.C), the Kolliker-Fuse(KF) nucleus and the rostral ventrolateral medulla(RVLM), whereas 5-HT immunopositive and HRP double-labeled(5-HT-HRP double labeled) neurons were restricted mainly in the nucleus raphe pallidus(RP) and RVLM. These findings may indicate the GABAergic neurons in the RM,KF and RVLM, and 5-HT neurons in the RP and RVLM send monosynaptic axonal projections to the phrenic motor nucleus, it is suggested that GABAergic and 5-HT neurons have inhibitory and excitatory controls respectively, on phrenic motoneuron excitability.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 青木 藩: "Projections of GABAergic and 5-HT neurons in the medullary raphenuclei in the cat"Jpn.J.Physiol.. 50・Suppl. S125 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 青木 藩: "肋間筋および横隔膜運動"神経内科. 55・1. 1-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 青木 藩, 松山清治: "延髄大縫線核GABAニューロンによる呼吸性ニューロン発射抑制はGABAレセプターを介する"日本生理学雑誌. 64・10. 211 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki, M., Matsuyama, K.: "Projection from GABAergic and serotonergic neurons in the raphe nuclei to upper cervical neurons in cats"Jpn.J.Physiol.. 50・Suppl. S85 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki, M., Matsuyama, K., Song G: "Roles of medullary GABAergic and 5-HT neurons in respiratory control in cats"Jpn.J.Physiol.. 53・Suppl. S123 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki, M., Takeda, R.: "Neurotransmitters and modulators in the central respiratory network"Jpn.J.Physiol.. 53・Suppl. S122 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mamoru Aoki, Kiyoji Matsuyama: "Projections of GABAergic and 5-HT neurons in the medullary raphe nuclei in the cat."Jpn.J.Physiol.. 51(Suppl). 528 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mamoru Aoki: "Movements induced by intercostal muscles and diaphragm."Neurological Medicine(in Japanese). 55(1). 1-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mamoru Aoki, Kiyoji Matsuyama: "Projection from GABAergic and serotonergic neurons in the raphe nuclei to upper cervical neurons in cats."Jpn.J.Physiol.. 52(Suppl). 585 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mamoru Aoki, Kiyoji Matsuyama, Gang Song: "Roles of medullary GABAergic and 5-HT neurons in respiratory central in cats."Jpn.J.Physiol.. 53(Suppl). S123 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mamoru Aoki, Ryuji Takeda: "Neurotransmitters and modulators in the central respiratory network."Jpn.J.Physiol.. 53(Suppl). S122 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aoki, M., Matsuyama, K., Song G: "Roles of medullary GABAergic and 5HT neurons in respiratory control in cats"Jpn.J.Physiol.. 53・Luppl. S123 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Aoki, M., Takeda, R.: "Neurotransmitters and modulators in the central respiratory network"Jpn.J.Physiol.. 53・Luppl. S122 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 青木 藩, 宋 剛, 松山清治: "上部頸髄呼吸性ニューロンへの延髄縫線核GABA及び5-HTニューロンの投射"日本生理学雑誌. 64・1. 23 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 青木 藩, 松山清治: "延髄大縫線核GABAニューロンによる呼吸性ニューロン発射抑制はGABAレセプターを介する"日本生理学雑誌. 64・10. 211 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Aoki, M., Matsuyama, K.: "Projection from GABAergic and serotonergic neurons in the raphe nuclei to upper cervical neurons in cats"Jpn. J. Physiol.. 5O・Suppl. S85 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 青木 藩: "延髄縫線核GABA及び5-HTニューロンの横隔神経核への軸索投射"日本生理誌. 63・1. 42 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 青木 藩: "Projections of GABAergic and 5-HT neurons in the medullary raphe nuclei in the cat"Jpn. J. Physiol.. 50・(Suppl). S125 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 青木 藩: "肋間筋および横隔膜運動"神経内科. 55・1. 1-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Gang Song, Mamoru Aoki: "Frontiers in Modeling and Control of Breathing"Kluwer/Plenum Press, New York(C.S-Poon, H.Kazemi, eds). 107-112 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi