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Evaluation of the three dimendional microvascular structure and physiological function of renal glomerulus using superphoton derived X-ray micro focus CT

Research Project

Project/Area Number 13680959
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKawasaki Medical School

Principal Investigator

OGASAWARA Yasuo  Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)

Co-Investigator(Kenkyū-buntansha) FUJIMOTO Katsukuni  Kawasaki Medical School, Anatomy, Associate Professor, 医学部, 助教授 (80106351)
YADA Toyotaka  Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (00210279)
YAMAMOTO Tokunori  Kawasaki Medical School, Urology, Assistant Professor, 医学部, 講師 (20182636)
UMETANI Keiji  Japan Synchrotron Radiation Research Institute, Experimental Research Div., Chief Research, 放射線研究所実験部門, 主幹研究員(研究職) (50344396)
KAJIYA Fumihiko  Okayama University, Graduate School of Medical and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (70029114)
田中 啓幹  川崎医科大学, 医学部, 教授 (10069015)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordssuperphoton / X- ray micro focus CT / glomerulus / three dimentional structure / microcirculation / 三次元血管構造
Research Abstract

We aimed at visualizing thee dimensional (3D) glomerular micro- structures using a superphoton derived X- ray micro focus CT (Mct) to accurately measure glomerular volumes throughout a cortex and quantitatively evakuate global heterogeneity of the volume- distribution in a rat with spontaneous diabetes mellitus (DM). Using the Otsuka Long Evans Tokushima Fatty (OLETF) rats as rats of early stage DM (28 weeks, n=5) and control rats (Long Evans Tokushima Fatty; LETO, n=5) of the same age, the vessels of the left kidney was filled up with contrast media (BaSO_4 + India ink + 8%gelatin). A sampled renal column (3-4 mm) was observed using two Mct system; 8GeV- superphoton Mct for the highest image quality (3-6 mm in resolution) and commercially available Mct (10 mm in resolution) for data accumulation. The glomerular volumes more than 400 per sample were computed by coefficient variation (CV). By stereomicroscopic observation, we confirmed complete- filling of the vessels with the contrast media. The glomeruli in OLETF were characterized by irregular stereostractures, whereas those in LETO were characterized by regular glomerular structure. The CV of glomerular volume was significantly larger in OLETF than LETO (p<0.01). In addition, absolute glomerular volume was larger in OLETF than LETO (p<0.005). The normalized glomerular volume was, however, comparable (NS), indicating glomerular enlargement in early DM may associate with increase in body mass. Both Mct provided similar results. Highly heterogeneous glomerular volume- distribution in DM rat was quantitatively demonstrated using our developed 3D glomerular micro- structural visualizing method. This technique provides a new aspect for the evaluation of global heterogeneity in glomerular remodeling under the chronic renal dysfunction, which may provide more sensitive insight into early complications of diabetic nephropathy

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Yasuo Ogasawara: "Global heterogeneity of glomerular volume-distribution evaluated by three-dimensional analysis using synchrotron radiation X-ray microCT."SPring-8 Research Frontiers 2000/2001. 27-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuo Ogasawara: "Analysis of three dimensional architecture of renal microcirculation"SPring-8 User Experiment Report. 125 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tokunori Yamamoto: "In vivo visualization of angiotensin II-and tubuloglomerular feedback-mediated renal vasoconstriction"Kidney International. 60. 364-369 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tokunori Yamamoto: "Direct in vivo visualization of renal microcirculation by intravital CCD videomicroscopy"Experimental Nephrology. 9. 150-155 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keiji Umetani: "Observation of Cardiac Microcirculation Using High Speed Microangiography System"SPring-8 User Experiment Report. 161 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fumihiko Kajiya: "Tissue Engineering for Therapeutic Use 5"Elsevier Science B.V.. 12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuo Ogasawara: "Global heterogeneity of glomerular volume- distribution evaluated by three- dimensional analysis using synchrotron radiation X- ray micro CT"Spring- 8 Research Frontiers. 2000/2001. 27-28 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuo Ogasawara: "Analysis of three dimensional architecture of renal microcirculation"Spring- 8 User Experiment. Report. 125 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tokunori Yamamoto: "In vivo visualization of angiotensin II- and tubuloglomerular feedback- mediated renal vasoconstriction"Kidney International. 60. 364-369 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tokunori Yamamoto: "Direct in vivo visualization of renal microcirculation by intravital CCD videomicroscopy"Experimental Nephrology. 9. 150-155 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Keiji Umetani: "Observation of Cardiac Microcirculation Using High Speed Microangiography System"Spring-8 User Experiment. Report. 161 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fumihiko Kajiya: "Elsevier Science B. V."Tissue Engineering for Therapeutic Use 5. 12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuo Ogasawara: "Global heterogeneity of glomerular volume-distribution evaluated by threedimensional analysis using synchrotron radiation X-ray micro CT"SPring-8 Research Frontiers 2000/2001. 27-28 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuo Ogasawara: "Analysis of three dimensional architecture of renal microcirculation"SPring-8 User Experiment Report. 125 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山本徳則: "高血圧および糖尿病ラット病態モデルにおける生体腎微小循環の可視化"日本バイオレオロジー学会誌(B&R). 16・9. 91-100 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小笠原康夫: "micro-CTを用いた腎糸球体容量分布の解析"第16回生体・生理工学シンポジウム論文集. 195-196 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小笠原康夫: "腎微小循環系血管容量分布の解析-micro-CTによる糸球体の立体的可視化"第49回レオロジー討論会講演要旨集. 5-6 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小笠原康夫: "Micro-CTを用いた糖尿病性腎症の皮質内糸球体容量分布解析"医用電子と生体工学. 39・Suppl.. 490 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小笠原康夫: "腎微小循環系の三次元血管構造計測と解析-腎糸球体毛細血管-"第6回X線結像光学シンポジウムプログラム予稿集. 35-36 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuo Ogasawara: "Global heterogeneity of glomerular volume-distribution evaluated by three dimensional analysis using superphoton derived X-ray microCT"SPring-8 Research Frontiers 2000/2001. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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