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Evaluation of dynamic changes of nitric oxide and oxidative stress in hemodialysis patients

Research Project

Project/Area Number 13680961
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKawasaki College of Allied Health Professions

Principal Investigator

MOCHIZUKI Seiichi  Kawasaki College of Allied Health Professions, Medical Engineering, Associate Professor, 臨床工学科, 助教授 (60259596)

Co-Investigator(Kenkyū-buntansha) HIRAMATSU Osamu  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Assistant Professor, 臨床工学科, 講師 (50208849)
GOTO Masami  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Professor, 臨床工学科, 教授 (50148699)
KASHIHARA Naoki  Kawasaki Medical School, Dept.of Nephrology, Professor, 医学部, 教授 (10233701)
KIMOTO Masumi  Okayama Prefectural University, Dept.of Nutritional Science, Professor, 保健福祉学部, 教授 (40108866)
TACHIBANA Hiroyuki  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Research Associate, 臨床工学科, 助手 (00241216)
松本 健志  川崎医療短期大学, 臨床工学科, 助教授 (30249560)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordshemodialysis / nitric oxide (NO) / oxidative stress / superoxide / L-arginine / asymmetric dimethylarginine (ADMA) / tetrahydrobiopterin (BH_4) / oxidized LDL / スーパーオキサイド / テトラハイドロビオプテリン(BH_4) / asymmetric dimethylarginine(ADMA) / 血液汚染 / 高血圧 / ADMA / センサー
Research Abstract

Plasma concentrations of oxidized LDL and ADMA, both of which are markers of oxidative stress were measured. OxyLDL was significantly higher than healthy group and increased further by hemodialysis (HD). ADMA level was higher than healthy group before HD and gradually decreased in the time course of HD.
Next, we evaluated the plasma levels of NO-related factors in pathophysiological model rats (hypertension and diabetes mellitus). In both groups, plasma BH_4 level decreased, plasma ADMA level increased, and myocardial DDAH (ADMA hydrolase) activity decreased. Superoxide production of control rats did not differ among heart, kidney and liver, while it decreased significantly in those organs of hypertensive rats.
Systemic NO production rate in HD patients was estimated and the rate during HD was significantly higher than in the inter-HD period. There was a good linear correlation between L-arginine/ADMA and systemic NO production rate. Also the same between BH_4/BH_2 and systemic NO production rate. From a clinical viewpoint, variations of these parameters would affect significantly the NO production during HD, and thus control of these factors and other methods should be investigated further.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Mochizuki S, et al.: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart.Vessels. 15. 274-279 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mochizuki S, et al.: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovasc.Eng.Int.J.. 1. 85-91 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mochizuki S, et al.: "Evaluation of basic performance and applicability of a newly developed in vivo nitric oxide sensor"Physiol.Meas.. 23. 261-268 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Endo K, et al.: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors Actuator. 83. 30-34 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyasaka T, et al.: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in dialysate"ASAIO J.. 48. 389-393 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 望月精一: "冠循環のバイオメカニクス(梶谷文彦編著):ノート(メカノストレスとNO、EDHF、CO、PGI_2)"コロナ社. 222 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart.Vessels. 15(6). 274-279 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovasc.Eng.Int.J.. 1(2). 85-91 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Evaluation of basic performance and applicability of a newly developed in vivo nitric oxide sensor"Physiol.Meas.. 23(2). 261-268 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyotaka Yada, et al.: "Roles of ADMA, BH4, oxidative stress as risk factors and antioxidant effect of an HMG-CoA reductase inhibitor in insulin-resistant Zucker fatty rats"Journal of Japanese College of Angiology. 42(5). 297-300 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kosuke Endo, et al.: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors Actuator. 83. 30-34 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takehiro Miyasaka, et al.: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in dialysate"ASAIO J.. 48. 389-393 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Measurement of acetylcholine-induced endothelium-derived nitric oxide in aorta using a newly developed catheter-type nitric oxide sensor"Biochem.Biophys.Res.Commun.. 306. 505-508 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masayo Hayama, Takehiro Miyasaka, Seiichi Mochizuki, et al.: "Optimum dialysis membrane for endotoxin blocking"J.Memb.Sci.. 219. 15-25 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release"Am.J.Physiol.Heart Circ.Physiol.. 285. H722-H726 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Measurement of acetylcholine-induced endothelium-derived nitric oxide in aorta using a newly developed catheter-type nitric oxide sensor"Biochemical and Biophysical Research Communications. 306. 505-508 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masayo Hayama, Takehiro Miyasaka, Seiichi Mochizuki, et al.: "Optimum dialysis membrane for endotoxin blocking"Journal of Membrane Science. 219. 15-25 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seiichi Mochizuki, et al.: "Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release"American Journal of Physiology. 285. H722-H726 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 遠藤恒介, 宮坂武寛, 望月精一他: "スーパーオキサイドセンサ"Medical Science Digest. 29・14. 20-23 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 氷見直之, 宮坂武寛, 望月精一他: "スーパーオキサイド産生量の正常と病態ラットにおける臓器間差異の解析"信学技報. MBE2003-50. 43-46 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 望月精一他: "血液透析に関連した病態におけるNO・酸化ストレスの関連因子の計測・評価"腎臓. 26・2. 172-176 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 矢田豊隆: "内因性NO合成阻害因子と酸化ストレス起因性血管内皮障害の発症メカニズムとHMG-CoA reductase inhibitor長期投与の効果"脈管学. 42・5. 297-300 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kosuke Endo: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors and Actuator. 83. 30-34 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takehiro Miyasaka: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in"ASAIO Journal. 48. 389-393 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 宮坂武寛: "生体計測用スーパーオキサイドセンサ"医工学治療. 14・3. 158-161 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seiichi Mochizuki: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart and Vessels. 15・6. 274-279 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seiichi Mochizuki: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovascular Engineering : An Internatinal Journal. 1・2. 85-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seiichi Mochizuki: "Evaluation of basic performance and applicability of a newly developed in vivo nitric oxide sensor"Physiological Measurement. 23・2. 261-268 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2020-01-17  

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