• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of a transcription factor, VEZF1 in endothelial differentiation and vasculogenesis

Research Project

Project/Area Number 13832001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionTohoku University

Principal Investigator

ABE Mayumi  Institute of Development, Aging and Cancer Assistant professor, 加齢医学研究所, 助手 (80271980)

Co-Investigator(Kenkyū-buntansha) SATO Yasufumi  Institute of Development, Aging and Cancer Professor, 加齢医学研究所, 教授 (50178779)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsVEZF1 / etidotheital cells / transcription factors / vaculogenesis / angiogenesis / ES cells / differentiation / VEZF / 内皮細胞 / 胚性幹細胞 / 血管内皮前駆細胞
Research Abstract

1. We made VEZF1 sense and antisense expression vectors (VEZF1-S and VEZF1-AS) by inserting the VEZF1 cDNA-IRES-EGFP fragment into pHPCAG, a stable expression vector for ES cells to monitor the transfection efficiency and introduced the vector intoMG1.19, an ES cell line by a supertransfection method.
2. We found that VEZF1 was expressed in MG1.19 cultured on gelatin in the presence of LIF : in the undiflerentiation system.
3. Although we used IRES-EGFP in pHPCAG as a Mock at first, EGFP expression was not detected in the vector. Therefore, we use EGFP in pHPCAG as a Mock.
4. EGFP was detected in Mock, VEZF1-S, and VEZF1-AS by FCM. However the expression level of VEZF1 measured by RT-PCR and Western blot was the same among these three transfectants.
5. Furthermore, the expression of VEGFR2 (Flk-1), a marker of endothelial progenitor cells was the same in these three transfectants on OP9 cells without LIF: in the differentiation system.
6. We found VEZF1 was essential for endothetial growth, migration, and network formation in Vitro and in Vivo (our unpublished data). However, we could not clarify the involvement of VEZF1 in endothelial differentiation as well as in angiogenesis because of failure in inhibiting VEZF1 expression by VEZF1-AS.
7. We would like to try using siRNA.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] M.Oikawa, M.Abe, H.Kurosawa, W.Hida, K.Shirato, Y.Sato: "Hypoxia induces transcription factor ETS-1 via the activity of Hypoxia-inducible factor-1"Biochem Biophys Res Commun. 289. 39-43 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Terai, M.Abe, K.Miyamoto, M.Koike, M.Yamasaki, M.Ueda, M.Ueki, Y.Sato: "Vascular smooth muscle cell growth-promoting factor/F-spondin inhibits angiogenesis via the blockade of integrin avβ3 on vascular endothelial cells"J. Cell. Physiol.. 188. 394-402 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Abe, Y.Sato: "cDNA microarray analysis of the gene expression profile of VEGF-induced human umbilical vascular endothelial cells"Angiogenesis. 4. 289-298 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Abe, D.Inoue, K.Matsunaga, Y.Ohizumi, H.Ueda, T.Asano, M.Murakami, U.Sato: "Goniodomin A, an antifungal polyether macrolide, exhibits antiangiogenic activities via inhibition of actin reorganization in endothelial cells"J. Cell. Physiol.. 190. 109-116 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Abe, N.Oda, K.Shibata, M.Yamasakiand, Y.Sato: "Augmented binding and activation of latent transforming growth factor-β by a tryptic fragment of latency associated peptide"Endothelium. 9. 25-36 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Nakagawa, M.Abe, T.Yamazaki, H.Miyaahita, H.Niwa, S.Kokubun, Y.Sato: "HEX Acts as a Negative Regulator of Angiogenesis by Modulating the Expression of Angiogenesis-Related Gene in Endothelial Cells In Vitro"Arterioscle. Thromb. Vase. Biol.. 23. 231-237 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Oikawa: "Hypoxia induces transcription factor ETS-1 via the activity of Hypoxia-inducible factor-1"Biochem Biophys Res Commun.. 289(1). 39-43 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Terai: "Vascular smooth muscle cell growth-promoting factor/F-spondin inhibits angiogenesis via the blockade of integrin avβ3 on vascular endothelial cells"J. Cell. Physiol. 188. 394-402 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Abe: "cDNA microarray analysis of the gene expression profile of VEGF-induced human umbilical vascular endothelial cells"Angiogenesis. 4. 289-298 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Abe: "Goniodomin A, an antifungal polyether macrolide, exhibits antiangiogenic activities via inhibition of actin reorganization in endothelial cells"J. Cell. Physiol.. 190. 109-116 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Abe: "Augmented binding and activation of latent transforming growth fector-β by a tryptic fragment of latency associated peptide"Endothelium. 9. 25-36 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Nakagawa: "HEX Acts as a Negative Regulator of Angibgenesis by Modulating the Expression of Angiogenesis-Related Gene in Endothelial Cells In Vitro"Arterioscle. Thromb. Vase. Bioh.. 23. 231-237 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe M., Inoue D., Matsunaga K., Ohizumi Y., Ueda H., Asano T., Murakami M., Sato Y.: "Goniodomin A, an antifungal polyether macrolide, exhibits antiangiogenic activities via inhibiting actin reorganization of endothelial cells"J.Cell.Physiol.. 190. 109-116 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe M., Oda N., Shibata K., Yamasaki M., Sato Y.: "Augmented binding and activation of latent transforming growth factor-β by a tryptic fragment of latency associated peptide"Endothelium. 9. 25-36 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakagawa T., Abe M., Yamazaki T., Miyashita H., Niwa H., Kokubun S., Sato Y.: "HEX acts as a negative regulator of angiogenesis by modulating the expression of angiogenesis-related gene in endothelial cells in vitro"Arterioscl.Thromb.Vasc.Biol.. 23. 231-237 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi