| Project/Area Number |
13832006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Kyushu University |
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Principal Investigator |
NISHIMURA Junji Graduate School of Medical Sciences, Kyushu University, Associate Prof., 大学院・医学研究院, 助教授 (90237727)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Katsuya Graduate School of Medical Sciences, Kyushu University, Lecturer, 大学院・医学研究院, 講師 (80291516)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Vascular smooth muscle / Contraction / Virus vector / Collagen gel / RhoA / Cell culture |
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| Research Abstract |
The purpose of the present study was to investigate the mechanism underlying the contractility of the proliferating airway smooth muscle. We could obtain the following results.
1, Wd could construct the recombinant baculo virus vector, which can be infected with a high efficiency to the mammalian cell, by inserting the cytomegalo virus promoter and the cDNA of interest. This virus could successfully transfected to the NIH 3T3 fibroblast, porcine tracheal smooth muscle cells and porcine coronary smooth muscle cells. It took us long time to optimize the condition for the transfection. However, we could overcome this problem by modifying the purification process of the recombinant baculo virus.
2, We could also establish the method to measure isometric tension using the cultured smooth muscle cells by reconstituting these cells into tissue-like preparation. The RhoA transfected cells showed 2-3 times greater contractility, compared with the control treatment.
3, On the contrary, the knock-down of RhoA expression by RNA interference greatly inhibited the smooth muscle contractility.
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