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Development of multi-generation assay for endocrine disrupting chemicals

Research Project

Project/Area Number 13833009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 放射線・化学物質影響科学
Research InstitutionAriake National College of Technology

Principal Investigator

TOMINAGA Nobuaki  Ariake National College of Technology, Department of Chemical and Biological Engineering, Associate Professor, 物質工学科, 助教授 (30227631)

Co-Investigator(Kenkyū-buntansha) NAGASAKI Kohra  Shinya University, Faculty of Environmental Sciences, Professor, 環境科学部, 教授 (40225389)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsendocrine disruptor / C.elegans / bioassay / reproduction / multi-generation / 内分泌攪乱物質 / ビスフェノールA / 環境ホルモン / エストロジェン / アンドロジェン
Research Abstract

In this study, we investigated to develop a multiple-generation toxicity assay for biological evaluation of chemicals. The nematode Caenorhabditis elegans was adopted for a model organism. Then we examined the sublethal toxicity, fecundity and reproduction for over five generations. As the results, C. elegans responds to vertebrate steroid hormones. Especially estradiol has a cholesterol-like potency in supporting the reproduction of C. elegans. In contrast testosterone and diethylstibestrol suppressed the fecundity rate of C. elegans. Some chemicals were evaluated by same method. In particular, bisphenol A was decreased the number of C. elegans significantly even at 1 nM. A comparison of the number of viable worms and eggs suggested that the phenols exert hatching toxicity.
When we performed western-blotting analyses using specific antibodies for phosphorylated amino acids, we could detect some proteins which phosphorylation levels were changed in estradiol exposed C. elegans extracts.
These results indicated that this toxicity assay had 100-1000-fold higher sensitivity than the LC50s and free living nematode C. elegans is becoming an effective model organism for testing chemical toxicity including reproductive disruption.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Nobuaki Tominaga, Shinya Kohra, Taisen Iguchi, Koji Arizono: "A multi-generation sublethal assay of phenols using the nematode Caenorhabditis elegans"J.Health Science. 49(6). 459-463 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Kazuhiro Ura, Masato Kawakami, Tomoaki Kawaguchi, Yoshinori Mitsui, Taisen Iguchi, Koji Arizono: "Caenorhabdotis elegans repose to specific steroid Hormones"J.Health Science. 49(1). 28-33 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Manabu Kunimoto, Toshinori Kai, Koji Arizono, Shinya Kohra: "A convienient acute toxicity assay using C.elegans as a model organism.-Application for alkylphenol toxicity-"Environmental Sciences. 10(4). 215-221 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Mineko Tomoeda, Shinya Kohra, Yuji Takao, Masaki Nagae, Kazuo Ueda, Yasuhiro Ishibashi, Toshinori Kai, Koji Arizono: "A convienient sublethal assay of alkylphenol and organotin compounds using the nematode Caenorhabditis elegans."J.Health Science. 48(6). 555-559 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsuya Matsuno, Kazuhiro Ura, Risa Sonoda, Yuji Kohara, Hiroko Uesugi, Koji Arizono, Taisen Iguchi, Nobuaki Tominaga: "Sensing of chemical substances using gene expression patterns in C.elegans."Sensors & Materials. 14(7). 395-406 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinya Kohra, Nobuaki Tominaga, Yuji Takao, Masaki Nagae, Yasuhiro Ishibashi, Kazuo Ueda, Koji Arizono: "A rapid respiratory toxicity test using Caenorhabditis elegans with an oxygen electrode system"J.Health Science. 48(3). 269-272 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Shinya Kohra, Taisen Iguchi, Koji Arizono: "A multi-generation sublethal assay of phenols using the nematode Caenorhabditis elegans"Journal of Health Science. 49(6). 459-463 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Kazuhiro Ura, Masato Kawakami, Tomoaki Kawaguchi, Shinya Kohra, Yoshinori Mtsui, Taisen Iguchi, Koji Arizono: "Caenorhabdotis elegans respose to specific steroid Hormones"Journal of Health Science. 49(1). 28-33 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Manabu Kunimoto, Toshinori Kai, Koji Arizono, Shinya Kohra: "A convienient acute toxicity assay using C. elegans as a model organism.-Application for alkylphenol toxicity"Environmental Sciences. 10(4). 215-221 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Mineko Tomoeda, Shinya Kohra, Yuji Takao, Masaki Nagae, Kazuo Ueda, Yasuhiro Ishibashi, Toshinori Kai, Koji Arizono: "A convienient sublethal assay of alkylphenol and organotin compounds using the nematode Caenorhabditis elegans"Journal of Health Science. 48(6). 555-559 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tetsuya Matsuno, Kazuhiro Ura, Risa Sonoda, Yuji Kohara, Hiroko Uesugi, Koji Arizono, Taisen Iguchi, Nobuaki Tominaga: "Sensing of chemical substances using gene expression patterns in C. elegans"Sensors & Materials. 14(7). 395-406 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinya Kohra, Nobuaki Tominaga, Yuji Takao, Masaki Nagae, Yasuhiro Ishibashi, Kazuo Ueda, Kqji Arizono: "A rapid respiratory toxicity test using Caenorhabditis elegans with an oxygen electrode system"Journal of Health Science. 48(3). 262-272 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinya Kohra, Kazuko Kuwahara, Yuji Takao, Yasuhiro Ishibashi, Ho Chul Lee, Koji Arizono, Nobuaki Tominaga: "Effect of Bisphenol A on the feeding behavior of Caenorhabditis elegans."Journal of Health Science. 48(1). 93-95 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Tominaga, Shinya Kohra, Taisen Iguchi, Koji Arizono: "A multi-generation sublethal assay of phenols using the nematode Caenorhabditis elegans"J Health Science. 49(6). 459-463 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hideaki Shimada, Nobauki Tominaga, Shinya Kohra, Yoshinori Mitsui, Koji Arizono: "Metallothionein gene expression in the larvae of Caenorhabditis elegans is a potential biomarker for cadmium and mercury"Trace elements and electrolytes. 20(4). 240-243 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shinya Kohra, Nobuaki Tominaga, Yuji Takao, Masaki Nagae, Yasuhiro Ishibashi, Kazuo Ueda, Koji Arizono: "A rapid respiratory toxicity test using Caenorhabditis elegans with an Oxygen electrode SyStem"J. Health Science. 48(3). 269-272 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nobuaki Tominaga, Mineko Tomoeda, Shinya Kohra, Yuji Takao, Masaki Nagae, Kazuo Ueda, Yasuhiro Ishibashi, Toshinori Kai, Koji Arizono: "A convienient sublethal assay of alkylphenol and organotin compounds using the nematode Caenorhabditis elegans"J. Health Science. 46(6). 555-559 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nobuaki Tominaga, Kazuhiro Ura, Masato Kawakami, Tomoaki Kawaguchi, Shinya Kohra, Yoshinori Mitsui, Taisen Iguchi, Koji Arizono: "Caenorhabditis elegans resposes to specific steroid hormones"J. Health Science. 49(1). 28-33 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Kohra et al.: "Effect of BisphenolA on the feeding behavior of Caenorhabditis elegans"Journal of Health Science. 48・1. 93-95 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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