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Geographic Pattern of the Microsatellite DNA Polymorphism in two species of Sorex shrew in Hokkaido

Research Project

Project/Area Number 13839002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OHDACHI Satoshi  Hokkaido Univ., Inst. Low Temp. Sci., Inst., 低温科学研究所, 助手 (60292041)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsShrew / Microsatellite DNA / Cytochrome b / Genetic structure / Northeastern Asia / 個体群 / 北海道
Research Abstract

The biogeographic history of six soricine shrew species inhabiting the Japan archipelago and neighboring regions has been studied based on intra- and interspecific phylogeny of nucleotide sequences of mitochondrial cytochrome b gene.
To infer biogeographic histories of S. caecutiens and S. unguiculatus in more detail, phylogenetic relationships between species or populations were investigated by restriction fragment length polymorphism (RFLP) for the nuclear ribosomal RNA genes (rDNA). Genetic population structures were examined using microsatellite DNA markers.
From the rDNA-RFLP analysis, it was suggested in S. caecutiens that Hokkaido population is genetically distant from Eurasian ones and Sakhalin population originated from hybridization between the continent and Hokkaido populations.
High genetic variations in microsatellite loci were observed among Hokkaido mainland populations of both species. The allelic richness and heterozygosity were proportional to the island size and positiv … More e correlation between genetic and geographic distances was detected in S. unguiculatus, while S. caecutiens showed no positive correlation between genetic and geographic distances among local populations in Hokkaido mainland. This implies that the final population expansion in Hokkaido occurred in S. unguiculatus earlier than in S. caecutiens.
Combining the information from rDNA-RFLP, microsatellite DNA and mtDNA cyt-b, biogeographic histories of S. unguiculatus and S. caecutiens in Hokkaido were inferred as follows: Sorex caecutiens, which invaded Hokkaido before the last glacial age, was restricted to the forest refuge in southern Hokkaido because of its sylvicolous nature, at the maximum stage of last glacial age, where grassland vegetation prevailed in Hokkaido. However, it expanded its range with recovery of forest vegetation all over Hokkaido in post glacial age. S. unguiculatus invaded Hokkaido later than S. caecutiens, probably at the maximum stage of last gracial age, and soon came distributed throughout Hokkaido inhabiting not only the southern forest refuge but also grasslands prevaily under the cold climate. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Ohdachi, S., N.E.Dokuchaev, M.Hasegawa, R.Masuda: "Intraspecific phylogeny and geographic variation of six species of northeastern Asiatic Sorex shrews based on the mitochondrial cytochrome b nucleotide sequences"Molecular Ecology. 10. 2199-2213 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsubara, K, Y.Ishibashi, S.Ohdachi, Y.Matsuda: "A new primer set for sex identification in the genus Sorex (Soricidae, Insectivora)"Molecular Ecology Notes. 1. 241-242 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nesterenko, V., S.D.Ohdachi: "Postnatal growth and development in Sorex unguiculatus"Mammal Study. 26. 145-148 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwasa, M.A., S.Ohdachi, S.-H.Han, H.-S.Oh, H.Abe, H.Suzuki: "Karyotype and RFLP of the nuclear rDNA of Crocidura sp. on Cheju Island, South Korea (Mammalia, Insectivora)"Mammalia. 65. 451-459 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Han S.-H., M.A.Iwasa, S.D.Ohdachi, H.-S.Oh, H.Suzuki, K.Tsuchiya, H.Abe: "Molecular phylogeny of Crocidura shrews in northeastern Asia : a special reference to specimens on Cheju Island, South Korea"Acta Theriologica. 47. 369-379 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naitoh, Y, Y.Ishibashi, S.Abe, S.D.Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohdachi, S.D., H.Abe, S.-H.Han: "Phylogenetical Positions of Sorex sp. (Insectivora, Mammalia) from Cheju Island and S. caecutiens from the Korean Peninsula, Inferred from Mitochondrial Cytochrome b Gene Sequences"Zoological Science. 20. 91-95 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohdachi, S.D., M.A.Iwasa, V.A.Nesterenko, H.Abe, R.Masuda, W.Haberl: "Molecular Phylogenetics of Crocidura shrews (Insectivora) in East and Central Asia"Journal of Mammalogy. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohdachi, S., N. E. Dokuchaev, M. Hasegawa, R. Masuda: "Intraspecific phylogeny and geographic variation of six species of northeastern Asiatic Sorex shrews based on the mitochondrial cytochrome b nucleotide sequences"Molecular Ecology. 10. 2199-2213 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsubara, K, Y. Ishibashi, S. Ohdachi, Y. Matsuda: "A new primer set for sex identification in the genus Sorex (Soricidae, Insectivora)"Molecular Ecology Notes. 1. 241-242 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nesterenko, V., S. D. Ohdachi: "Postnatal growth and development in Sorex unguiculatus"Mammal Study. 26. 145-148 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwasa, M. A., S. Ohdachi, S. -H. Han, H. -S. Oh, H. Abe, H. Suzuki: "Karyotype and RFLP of the nuclear rDNA of Crocidura sp. On Cheju Island, South Korea (Mammalia, Insectivora)"Mammalia. 65. 451-459 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Han S. -H., M. A. Iwasa, S. D. Ohdachi, H. -S. Oh, H. Suzuki, K. Tsuchiya, H. Abe: "Molecular phylogeny of Crocidura shrews in northeastern Asia: a special reference to specimens on Cheju Island, South Korea"Acta Theriologica. 47. 369-379 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naitoh Y, Y. Ishibashi, S. Abe, S. D. Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohdachi, S. D., H. Abe, S. -H. Han: "Phylogenetical Positions of Sorex sp. (Insectivora, Mammalia) from Cheju Island and S. caecutiens from the Korean Peninsula, Inferred from Mitochondrial Cytochrome b Gene Sequences"Zoological Science. 20. 91-95 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohdachi, S. D., M. A. Iwasa, V. A. Nesterenko, H. Abe, R. Masuda, W. Haberl: "Molecular Phylogenetics of Crocidura shrews (Insectivora) in East and Central Asia"Journal of Mammalogy. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nesterenko, V., S.D.Ohdachi: "Postnatal growth and development in Sorex unguiculatus"Mammal Study. 26. 145-148 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwasa, M.A., S.Ohdachi, S.-H.Han, H.-S.Oh, H.Abe, H.Suzuki: "Karyotype and RFLP of the nuclear rDNA of Crocidura sp. on Cheju Island, South Korea (Mammalia, Insectivora)"Mammalia. 65. 451-459 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Han S.-H., M.A.Iwasa, S.D.Ohdachi, H.-S.Oh, H.Suzuki, K.Tsuchiya, H.Abe: "Molecular phylogeny of Crocidura shrews in northeastern Asia : a special reference to specimens on Cheju Island, South Korea"Acta Theriologica. 47. 369-379 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Naitoh Y, Y.Ishibashi, S.Abe, S.D.Ohdachi: "Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S.caecutiens"Molecular Ecology Notes. 2. 434-436 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.D.Ohdachi, H.Abe, S.-H.Han: "Phylogenetical Positions of Sorex sp. (Insectivora, Mammalia) from Cheju Island and S.caecutiens from the Korean Peninsula, Inferred from Mitochondrial Cytochrome b Gene Sequences"Zoological Science:91-95. 20. 91-95 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohdachi, S., N.E.Dokuchaev, M.Hasegawa, R.Masuda: "Intraspecific phylogeny and geographic variation of six species of northeastern Asiatic <Sorex>___- shrews based on the mitochondrial cytochrome b nucleotide sequences"Molecular Ecology. 10. 2199-2213 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsubara K., Ishibashi Y., Ohdachi S., Matsuda Y.: "A new primer set for sex identification in the genus <Sorex>___-(Soricidae,Insectivora)"Molecular Ecology Notes. 1. 241-242 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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