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生殖毒性評価・後代検定技術としての生殖細胞系列の完全連続培養技術の開発

Research Project

Project/Area Number 13876059
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionTohoku University

Principal Investigator

佐藤 英明  東北大学, 大学院・農学研究科, 教授 (80093243)

Co-Investigator(Kenkyū-buntansha) 松本 浩道  東北大学, 大学院・農学研究科, 助手 (70241552)
佐々田 比呂志  東北大学, 大学院・農学研究科, 助教授 (90158931)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Keywordsヒアルロン酸 / ヒアルロン酸合成酵素遺伝子 / ヒアルロン酸結合タンパク質 / CD44 / ギャップジャンクション / MAPキナーゼ / Fas / Fasリガンド / 卵子形成 / HAS2 / HAS3 / fas欠損マウス / アポトーシス
Research Abstract

1)卵子の退行抑制、成熟促進、血管増殖に係わるヒアルロン酸合成酵素遺伝子やヒアルロン酸結合タンパク質の解析を行い、HAS1、HAS2を卵丘細胞に、HAS3を卵母細胞に同定するとともにその構造を決定した。また、HAS2の発現調節領域の構造を決定した。ヒアルロン酸・CD44のシグナルがギャップジャンクション蛋白質Cx43のチロシンリン酸化を誘導し、ギャップジャンクションを閉鎖させるカスケードを明らかにした。ギャップジャンクションの閉鎖がどのように卵成熱を誘起するかについていくつかの仮説をたて解析を進めている。
2)卵子・卵丘細胞におけるCD44の分布を明らかにした。
3)HAS2遺伝子の転写調節領域に卵胞刺激ホルモンに応答する領域があると予想し、解析し、構造を決定した。
4)Fas・Fasリガンドシステムが卵胞で発現し、卵子の死滅を誘導することをマウスとブタですでに明らかにした。さらに透明帯にFas・Fasリガンドシステムの発現を抑制する効果があることを明らかにし、このような透明帯の機能を担う分子の同定に努力し、ヒアルロン酸であることを明らかにした。
5)始原生殖細胞におけるIgf2rのインプリンティングのプロファイルと調節系を明らかにした。
6)cAMPの減数分裂再開始抑制が受精能・発生能の上昇に係わることを明にした。
7)マウス体細胞に胚性幹細胞の細胞質を導入し、多分化能をもつ細胞に分化させることに成功した。

Report

(3 results)
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 佐藤 英明: "Progesterone activation of parthenogenones and their potential for development in mouse oocytes."Animal Frontier Sciences- Life science update in animal science-. 61-68 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 英明: "Porcine embryonic stem cell lines ; Possibility of medical applications and strategy for production"Animal Frontier Sciences- Life science update in animal science-. 219-223 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 英明: "Erasure of methylation imprinting of Igf2r during mouse primordial germ-cell development."Mol.Reprod.Dev.. 65. 41-50 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 英明: "Meiotic arrest maintained by cAMP during the initiation of maturation enhances meiotic potential and developmental competence and reduces polyspermy of IVM/IVF porcine oocytes."Zygote. 11・1. 199-206 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 英明: "Successful production of blastocysts following ultrarapid vitrification with step-wise equilibriation of germinal vesicle-stage mouse oocytes."J.Reprod.Dev.. 49・6. 501-506 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 英明: "Determination of optimal conditions for parthenogenetic activation and subsequent development of rat oocytes in vitro."J.Reprod.Dev.. 50・1. 139-146 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 英明: "哺乳類の卵細胞"朝倉書店(印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤英明: "Expression of hyaluronan synthases and CD44 messenger RNAs in porcine cumulus-oocyte complexes during in vitro maturation"Biology of Reprodction. 66・4. 707-717 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "Induction of apoptosis mediated by Fas receptor and activation of caspase-3 in MRL-+/^* or MRL-lpr. lpr murine oocytes"Zygote. 10・1. 17-22 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "Identification of hyaluronic acid-binding proteins in proteins and their expressions in porcine cumulus-oocyte complexes during in vitro maturation"Biology of Reproduction. 66・6. 1165-1171 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "Expression of CD44 in porcine cumulus-oocyte complexes during in vitro oocyte maturation"Journal of Mammalian Ova Research. 19・2. 121-127 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "Distribution and gene expression of cytoskeletal proteins in two-cell rat embryos and developmental arrest"Journal of Experimental Zoology. 293・4. 641-648 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "Erasure of methylation imprinting of Igf2r during mouse primordial germ-cell development"Molecular Reproduction and Development. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "図説ARTマニュアル"永井書店. 509 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤英明: "Establishment of an in vitro production system for unclear transfer embryos reconstituted with cultured cells in pigs"J.Reprod.Dev. 47(Suppl.1). 41-45 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤英明: "Molecular basis of oocyte maturation in culture in the pig"J.Reprod.Dev. 47(Suppl.1). 63-69 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤英明: "Characteristics of infertility in female hypothyroid(hyt) mice"Reproduction. 122. 695-700 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤英明: "Parthenogenetic activation and subsequent development of rat oocytes in vitro"Mol.Reprod.Dev. 61. 120-125 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤英明: "Evaluation of bull fertility by migration of frozen-thawed and washed sperm in medium containing cervical mucus"J.Reprod.Dev. 47. 393-398 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤英明: "Vitrification of large quantities of immature bovine oocytes using nylon mesh"Cryobiology. 42. 139-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤英明: "卵子研究法"養賢堂. 405 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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