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ツパイゲノム情報に基づく肝炎ウイルス動物感染系の確立に関する研究

Research Project

Project/Area Number 13F03079
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Laboratory animal science
Research InstitutionKagoshima University

Principal Investigator

小原 恭子  鹿児島大学, 共同獣医学部, 教授 (20225478)

Co-Investigator(Kenkyū-buntansha) EZZIKOURI Sayeh  鹿児島大学, 共同獣医学部, 外国人特別研究員
EZZIKOURI Sayeh  鹿児島大学, 共同獣医学部, 外国人特別研究員
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2014: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2013: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsツパイ / ゲノム / HCV / HBV / ピクノジュノール
Outline of Annual Research Achievements

①今年度は、100種以上のツパイの分子に対して抗体を作成し、その性状解析を行った。
②ツパイ新生児に対して、B型肝炎ウイルス(HBV)、C型肝炎ウイルス(HCV)を接種し、感染を成立させる事に成功した。
③HCVに対して、抗ウイルス効果を示す物質を探索したところ、フランス松の樹液であるピクノジュノールが細胞毒性を示す事なく、50ug/mLの濃度まで効果がある事が明らかとなった。また、最近開発された抗HCV薬でプロテアーゼ阻害剤であるテラプレビルは、非常に有効な抗HCV剤ではあるが、耐性ウイルスも出現する。ピクノジュノールをテラプレビル耐性ウイルスに作用させたところ、抗ウイルス効果をもつ事が明らかとなった(Ezzikouri et al., Antivirus Res., 2015)。以上の様に、本年度の研究からピクノジュノールは既存の抗ウイルス薬の効果を高める、極めて有用な天然物である可能性が明らかとなった。
④HBVに対してもピクノジュノールが有効であるかを検討し、試験管内でウイルス抗原を減少させる効果があるという予備的知見が得られた。さらに詳細を検討している。今後、ツパイ感染系での評価も予定している。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (21 results)

All 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Invited: 3 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Inhibitory Effects of Pycnogenol on Hepatitis C Virus Replication.2015

    • Author(s)
      Ezzikouri S, Nishimura T, Kohara M, Benjelloun S, Kino Y, Inoue K, Matsumori A, Tsukiyama-Kohara K
    • Journal Title

      Antiviral Research

      Volume: 113C Pages: 93-102

    • DOI

      10.1016/j.antiviral.2014.10.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tupaia belangeri as an Experimental Animal Model for Viral Infection.2014

    • Author(s)
      Tsukiyama-Kohara K, Kohara M
    • Journal Title

      Experimental Animal

      Volume: 63 Pages: 367-374

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Toll-like receptor 4 and hepatitis B infection: molecular mechanisms and pathogenesis.2014

    • Author(s)
      Zare-Bidaki M1, Tsukiyama-Kohara K, Kazemi Arababadi M
    • Journal Title

      Viral Immunolgy

      Volume: 27 Issue: 7 Pages: 321-326

    • DOI

      10.1089/vim.2014.0039

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Resistance to cyclosporin A derives from mutations in hepatitis C virus nonstructural proteins.2014

    • Author(s)
      Arai M, Tsukiyama-Kohara K, Takagi A, Tobita Y, Inoue K, Kohara M
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 23 Issue: 1 Pages: 56-62

    • DOI

      10.1016/j.bbrc.2014.04.053

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Recent insights into hepatitis B virus-host interactions.2014

    • Author(s)
      Ezzikouri S, Ozawa M, Kohara M, Elmdaghri N, Benjelloun S, Tsukivama-Kohara K.
    • Journal Title

      Journal of Medical Virology

      Volume: 86 Issue: 6 Pages: 925-32

    • DOI

      10.1002/jmv.23916

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] B-Cell-Intrinsic Hepatitis C Virus Expression Leads to B-Cell-Lymphoma genesis and Induction of NF-κB Signalling2014

    • Author(s)
      Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, Kohara M, Mizuochi T. Tsukiyama-Kohara K.
    • Journal Title

      PLoS One

      Volume: 9(3)

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A serine palmitoyltransferase inhibitor blocks hepatitis C virus replication in human hepatocytes.2013

    • Author(s)
      Katsume A, Tokunaga Y, Hirata Y, Munakata T, Saito M, Hayashi H, Okamoto K, Ohmori Y, Kusanagi I, Fujiwara S, Tsukuda T, Aoki Y, Klumpp K, Tsukivama-Kohara K, El-Gohary A, Sudoh M, Kohara M.
    • Journal Title

      Gastroenterology

      Volume: 145(4) Issue: 4 Pages: 865-79

    • DOI

      10.1053/j.gastro.2013.06.012

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] A comprehensive study of B-lymphoma cells spontaneously developed in transgenic mice that express the full hepatitis C virus genome in B cells.2014

    • Author(s)
      Tsukiyama-Kohara K and Kohara M.
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Palmitoyltransferase Inhibitor Inhibits Hepatitis C Virus Replication in Human Hepatocytes.2014

    • Author(s)
      Kohara M, Tokunaga Y, Tsukiyama-Kohara K, Sudoh M.
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of Tupaia belangeri for HBV persistent infection.2014

    • Author(s)
      Ezzikouri, S., Chi, H.Y.., Sanada, T., Nagano, K., Yamaguchi, C.., Kanda, T., Kanazawa, N., Okuya, K., Ueno, K., Nakagawa, H., Nkogue, C.N., Ozawa, M., Miyoshi, N., Benjelloun, S., Murakami, S., Tanaka, Y., Kohara, M., Tsukiyama-Kohara, K.
    • Organizer
      第62回日本ウイルス学会
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Inhibitory Effects of Pycnogenol on Hepatitis C Virus Replication.2014

    • Author(s)
      Ezzikouri S., Kohara M, Tsukiyama-Kohara K.
    • Organizer
      第73回日本癌学会
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] HBV pathogenesis and host response in Tupaia belangeri2014

    • Author(s)
      Sanada T, Yamamoto N, Tsukiyama-Kohara K, Ezzikouri S, Tateno C, Kohara M
    • Organizer
      2014 HBV international Meeting
    • Place of Presentation
      UCLA, ロサンジェルス、アメリカ
    • Year and Date
      2014-09-03 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Comprehensive analysis of B-lymphoma cells spontaneously developed in transgenic mice that express the full hepatitis C virus genome in B cells2014

    • Author(s)
      Tsukiyama-Kohara K, Kohara M, and Kasama Y
    • Organizer
      第16回国際ウイルス学会(ICV2014)
    • Place of Presentation
      バンフ、カナダ
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Tumorigenicity inducued by hepatitis C virus.2014

    • Author(s)
      Tsukiyama-Kohara K., Kohara M. Tumorigenicity inducued by hepatitis C virus.
    • Organizer
      第4回新学術領域 発がんスパイラル国際シンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2014-02-11
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Development of Tupaia belangeri for small animal infection model of hepatitis B virus, according to the genomic research2013

    • Author(s)
      Tsukiyama-Kohara, K, Hirata, Y., Sanada T, Yamamoto N., Sayeh, E., Yasui F, Kohara, M.
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficacy of French maritime pine bark extract pycnogenolR to hepatitis C virus replication.2013

    • Author(s)
      EZZIKOURI S, Nishimura T, Ozawa M, Kohara M and Tsukiyama-Kohara K.
    • Organizer
      ウイルス学会九州支部会
    • Place of Presentation
      長崎
    • Year and Date
      2013-09-07
    • Related Report
      2013 Annual Research Report
  • [Presentation] C型肝炎ウイルス誘導性酸化ストレスによる肝細胞腫瘍原性亢進2013

    • Author(s)
      小原 恭子
    • Organizer
      第24回日本生体防御学会
    • Place of Presentation
      熊本
    • Year and Date
      2013-07-12
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chronic hepatitis C virus model in Tupaia belangeri2013

    • Author(s)
      Tsukiyama-Kohara K. Amako Y Kohara M.
    • Organizer
      Symposium HCV Animal models and vaccine d evelopment
    • Place of Presentation
      Estonia
    • Year and Date
      2013-05-17
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Development of tupaia nelangeri for small animal infection model of hepatitis B virus, according to the genomid research2013

    • Author(s)
      Tsukiyama-Kohara, K, Hirata, V., Sanada T, Yamamoto N., Yasui F. Kohara. M
    • Organizer
      HBV2013
    • Place of Presentation
      上海、中国
    • Related Report
      2013 Annual Research Report
  • [Book] 生命科学のためのウイルス学(監訳 下遠野邦忠、瀨谷司)2015

    • Author(s)
      小原恭子、小澤真(分担著者)
    • Total Pages
      332
    • Publisher
      南江堂
    • Related Report
      2014 Annual Research Report
  • [Remarks] 共同獣医学部動物衛生学分野ホームページ

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-douei/douei/index.php

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-01-29   Modified: 2024-03-26  

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