ハイスループット・トランスクリプトミクスを通じてのニューロン構造可塑性規制の分析

Research Project

Project/Area Number	13F03728
Research Category	Grant-in-Aid for JSPS Fellows
Allocation Type	Single-year Grants
Section	外国
Research Field	ゲノム生物学
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	CARNINCI Piero (2014) 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 副センター長 (10333296) CARNINCI Piero (2013) 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 副センター長
Co-Investigator(Kenkyū-buntansha)	KWON Tae-Jun 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員 KWON Tae・jun 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥2,300,000 (Direct Cost: ¥2,300,000) Fiscal Year 2014: ¥1,100,000 (Direct Cost: ¥1,100,000) Fiscal Year 2013: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords	Promoters / Transcriptome / Regulatory Analysis / Repeat analysis / Raw data processing and filtering / Data survey / Differential expression analysis / Clustering / Functional analysis
Outline of Annual Research Achievements	For the FY2014, we have completed the main bioinformatics analyses required for this project, using the collected transcriptome data from multiple Drosophila cell types at different stages of development using the CAGE experimental protocol. We decided to focus on: 1) Class IV da neuron development time course, and 2) specification of Class IV vs. the Class I-III cell types. We performed the following analyses: 1. Characterization of the expressed promoters based on their sequence composition and shapes (whether they are sharply peaked or broadly spread), and how they are differentially utilized. 2. Collecting and clustering of Drosophila-specific transcription factor binding site motifs and predicting their positions on the Drosophila genome. Due to the lack of available Drosophila-specific binding motifs, we collected and merged motifs based on their similarity. 3. Regulatory analysis of the differentially expressed promoters based on the collected motif set and available ChIP-seq and ChIP-chip data. We have come up with a list of candidate regulatory factors and are in the process of validating them experimentally. 4. Analysis of the repeat transcriptome for the analyzed samples. We found that certain repeats are differentially expressed according to developmental stages or cell types, and we are trying to determine what regulatory controls are behind these differences.
Research Progress Status	26年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	26年度が最終年度であるため、記入しない。