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荷電不安定性が引き起こす非平衡膜界面の新奇構造・機能

Research Project

Project/Area Number 13J01297
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field 生物物理・化学物理
Research InstitutionKyoto University

Principal Investigator

伊藤 弘明  京都大学, 理学研究科, 特別研究員(DC1)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2015: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2014: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2013: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords非平衡界面 / 生体界面 / 細胞骨格 / 赤血球 / アクトミオシン / 再構成 / flicker spectroscopy / ソフトマター / マイクロ流体デバイス / 界面 / 脂質膜 / 内毒素 / 界面透過法 / 人工細胞 / 反応拡散
Outline of Annual Research Achievements

敗血症因子に晒された赤血球は,シア流下での形状変化が健康な赤血球に比べて小さく,これが敗血症における全身性炎症の引き金になりうる事が知られていたが,弾性等の物性の変化の理解は不足している.そこで細胞形状ゆらぎのスペクトル解析とマイクロ流体デバイスや光トラップ等を統合して個々の赤血球の物性変化を定量した.内毒素分子の糖鎖が長い程作用した赤血球の曲げ弾性係数は減少すると同時にシア弾性係数は増加する事,新生児赤血球は成人赤血球よりこの変化が顕著である事,新薬候補ペプチドがこの変化を抑える事,を解明した.特に生体界面の特徴として,赤血球の形状ゆらぎの理解に細胞骨格に由来する結合項が不可欠である事を立証できた.また,外部から添加され細胞膜表面に作用する内毒素分子が細胞質側の細胞骨格のシア弾性に影響を与えるという結果は,細胞膜に埋め込まれた膜貫通タンパク質への作用を示唆しており,メゾスケールの形状ゆらぎと分子スケールの現象の関連の理解を深めるものである.
また,より動的で非平衡状態にある生体界面の振舞いの解明を目的として,アクチン・ミオシンタンパク質系(アクトミオシン)に駆動される再構成脂質膜界面の研究も並行してまとめた.アクトミオシンと脂質膜の結合に静電相互作用を援用することで,再構成したアクトミオシンの自発構造形成とカップルした人工脂質膜界面の動的変形が初めて観察されていたが,その解析や数理モデリングから界面変位の非熱的性質や曲率依存的な挙動が明らかになった.また,界面の非熱的ゆらぎの解析を深め,10秒スケールの時間相関をもつ有色ノイズ型の動的力生成が界面の非熱的変位に変換されていることを議論した.
上記の主要な成果に加え,荷電性界面の粗視化MD計算や多細胞生物ヒドラの界面変形など,階層横断的に非平衡界面現象の解明に取り組み成果を挙げた.

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (37 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 5 results) Presentation (27 results) (of which Int'l Joint Research: 7 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Non-periodic oscillatory deformation of an actomyosin microdroplet encapsulated within a lipid interface2016

    • Author(s)
      Yukinori Nishigami, Hiroaki Ito, Seiji Sonobe & Masatoshi Ichikawa
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1

    • DOI

      10.1038/srep18964

    • NAID

      120005758237

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tracking mechanical and morphological dynamics of regenerating Hydra tissue fragments using a two fingered micro-robotic hand2016

    • Author(s)
      M. Veschgini, F. Gebert, N. Khangai, H. Ito, R. Suzuki, T. W. Holstein, Y. Mae, T. Arai, and M. Tanaka
    • Journal Title

      Applied Physics Letters

      Volume: 108 Issue: 10 Pages: 1037021-4

    • DOI

      10.1063/1.4943402

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Quantification of the Influence of Endotoxins on the Mechanics of Adult and Neonatal Red Blood Cells2015

    • Author(s)
      Hiroaki Ito, Navina Kuss, Bastian E. Rapp, Masatoshi Ichikawa, Thomas Gutsmann, Klaus Brandenburg, Johannes M. B. Poeschl, and Motomu Tanaka
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 119 Issue: 25 Pages: 7837-7845

    • DOI

      10.1021/acs.jpcb.5b01544

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions2015

    • Author(s)
      M. Morita, H. Onoe, M. Yanagisawa, H. Ito, M. Ichikawa, K. Fujiwara, H. Saito and M. Takinoue
    • Journal Title

      ChemBioChem

      Volume: 16 Issue: 14 Pages: 2029-2035

    • DOI

      10.1002/cbic.201500354

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 試験管内再構築系を用いたブレブ駆動型アメーバ運動機構の研究 (Studies on bleb-driven cell locomotion using reconstituted systems)2015

    • Author(s)
      西上幸範、伊藤弘明、市川正敏
    • Journal Title

      植物科学の最前線(BSJ-Review)

      Volume: 6 Pages: 82-91

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wrinkling of a spherical lipid interface induced by actomyosin cortex2015

    • Author(s)
      Hiroaki Ito, Yukinori Nishigami, Seiji Sonobe and Masatoshi Ichikawa
    • Journal Title

      Physical Review E

      Volume: 92 Issue: 6

    • DOI

      10.1103/physreve.92.062711

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Coupling between pore formation and phase separation in charged lipid membranes2015

    • Author(s)
      Hiroki Himeno, Hiroaki Ito, Yuji Higuchi, Tsutomu Hamada, Naofumi Shimokawa, Masahiro Takagi
    • Journal Title

      Physical Review E

      Volume: 92 Issue: 6 Pages: 0627131-12

    • DOI

      10.1103/physreve.92.062713

    • NAID

      120005829720

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface2013

    • Author(s)
      H. Ito, T. Yamanaka, S. Kato, T . Hamada, M. Takagi, M. Ichikawa, and K. Yoshikawa
    • Journal Title

      Soft Matter

      Volume: 9 Issue: 40 Pages: 9539-9547

    • DOI

      10.1039/c3sm51766g

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] アクトミオシンと結合した再構成脂質界面のゆらぎと変形2016

    • Author(s)
      伊藤弘明、西上幸範、園部誠司、市川正敏
    • Organizer
      第6回京都若手ソフトマター研究会
    • Place of Presentation
      京都大学
    • Year and Date
      2016-03-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] アクトミオシンと結合した再構成脂質界面のゆらぎと変形2016

    • Author(s)
      伊藤弘明、西上幸範、園部誠司、市川正敏
    • Organizer
      新学術領域研究「ゆらぎと構造の協奏:非平衡系における普遍法則の確立」第2回若手勉強会「ソフトマター若手勉強会」
    • Place of Presentation
      東京大学検見川セミナーハウス
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] アクトミオシンによる力生成を実装した脂質界面のゆらぎと変形2016

    • Author(s)
      伊藤弘明
    • Organizer
      第3回分子ロボティクス若手の会
    • Place of Presentation
      京都大学iCeMS
    • Year and Date
      2016-02-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Interfacial deformation caused by reconstituted actomyosin2016

    • Author(s)
      H. Ito, Y. Nishigami, S. Sonobe, and M. Ichikawa
    • Organizer
      Kyoto Winter School 2016 "From Materials to Life: Multidisciplinary Challenges"
    • Place of Presentation
      京都大学
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Influence of endotoxin and anti-septic peptides on the mechanics of adult and neonatal red blood cells2015

    • Author(s)
      H. Ito, N. Kuss, B. Rapp, M. Ichikawa, T. Gutsmann, K. Brandenburg, J. Poeschl, and M. Tanaka
    • Organizer
      iCeMS International Symoposium Hierarchical Dynamics in Soft Materials and Biological Matter
    • Place of Presentation
      京都大学
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ultrafast detection of shape relaxation of human red blood cells passing through microchannels2015

    • Author(s)
      H. Ito, R. Murakami, S. Sakuma, C.-H. D. Tsai, M. Kaneko, and M. Tanaka
    • Organizer
      iCeMS International Symoposium Hierarchical Dynamics in Soft Materials and Biological Matter
    • Place of Presentation
      京都大学
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分子モーターに駆動された脂質単分子膜の非熱的ゆらぎ運動2015

    • Author(s)
      伊藤弘明、西上幸範、園部誠司、市川正敏
    • Organizer
      日本物理学会2015年秋季大会
    • Place of Presentation
      関西大学
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] ミクロ水滴から出発する自己駆動型人工細胞へのアプローチ(Approach to self-propelled artificial cells from microdroplets)2015

    • Author(s)
      伊藤弘明
    • Organizer
      日本生物物理学会第53回年会シンポジウム
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Impact of endotoxins on red blood cell mechanics and fluctuation2015

    • Author(s)
      H. Ito, N. Kuss, B. Rapp, M. Ichikawa, T. Gutsmann, K. Brandenburg, J. Poeschl, and M. Tanaka
    • Organizer
      Physics of Cell 2015 Conference
    • Place of Presentation
      Kloster Banz, Bad Staffelstein, Germany
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Impact of endotoxins on red blood cell mechanics and fluctuation2015

    • Author(s)
      H. Ito, N. Kuss, B. Rapp, M. Ichikawa, T. Gutsmann, K. Brandenburg, J. Poeschl, and M. Tanaka
    • Organizer
      Physics of Cell 2015 School
    • Place of Presentation
      Kloster Banz, Bad Staffelstein, Germany
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic fluctuation and deformation of lipid interface induced by actomyosin contractility2015

    • Author(s)
      Hiroaki Ito, Yukinori Nishigami, Seiji Sonobe, and Masatoshi Ichikawa
    • Organizer
      SFS2015 (International Symposium on Fluctuation and Structure out of Equilibrium 2015)
    • Place of Presentation
      京都大学
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reconstitution of contractile actomyosin cortex inside a cell-sized lipid interface2015

    • Author(s)
      Hiroaki Ito, Yukinori Nishigami, Seiji Sonobe, and Masatoshi Ichikawa
    • Organizer
      Engineering of Chemical Complexity 2015
    • Place of Presentation
      TUM Institute for Advanced Study (TUM-IAS), Munchen
    • Year and Date
      2015-06-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体高分子と相互作用する柔らかい膜の形態変化2015

    • Author(s)
      伊藤弘明、西上幸範、市川正敏
    • Organizer
      日本物理学会第70回年次大会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2015-03-21 – 2015-03-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生体高分子の構造形成と結合した柔らかい膜界面2015

    • Author(s)
      伊藤弘明
    • Organizer
      第5回京都若手ソフトマター研究会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-03-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Quantification of impacts of endotoxins on red blood cell membrane fluctuation2015

    • Author(s)
      Hiroaki Ito, Navina Kuss, Bastian Rapp, Masatoshi Ichikawa, Thomas Gutsmann, Klaus Brandenburg, Johannes Poeschl, Motomu Tanaka
    • Organizer
      Frontiers of Statistical Mechanics: From Non-equilibrium Fluctuations to Active Matter
    • Place of Presentation
      Kyoto University
    • Year and Date
      2015-02-04 – 2015-02-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞の動的振る舞いを目指した柔らかい界面の変形2014

    • Author(s)
      伊藤弘明
    • Organizer
      京都非線形ソフトマター研究会2014
    • Place of Presentation
      京都大原の里
    • Year and Date
      2014-10-17 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 油中水滴表面のゲル化に誘起される遊走と形状の相関2014

    • Author(s)
      伊藤弘明、市川正敏
    • Organizer
      日本物理学会2014年秋季大会
    • Place of Presentation
      中部大学
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 界面活性剤の会合体が誘起するミクロ水滴のアメーバ運動2014

    • Author(s)
      伊藤弘明
    • Organizer
      札幌非線形現象研究会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-08-27 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamical process on the formation of cell-sized vesicles through the adhesion of lipid monolayers2014

    • Author(s)
      Hiroaki Ito, Masatoshi Ichikawa, Kenichi Yoshikawa
    • Organizer
      Liquid Matter Conference 2014
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2014-07-21 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamical formation of lipid bilayer vesicles for synthesis of artificial cells2014

    • Author(s)
      Hiroaki Ito, Masatoshi Ichikawa, Kenichi Yoshikawa
    • Organizer
      Open Questions on the Origin of Life 2014
    • Place of Presentation
      International Institute for Advanced Studies, Kyoto
    • Year and Date
      2014-07-12 – 2014-07-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamical formation of cell-sized lipid bilayer vesicles for synthesis of artificial cells2014

    • Author(s)
      Hiroaki Ito
    • Organizer
      Development and future of artificial cells: Computation, fluctuation, and evolution
    • Place of Presentation
      Kyoto University
    • Year and Date
      2014-06-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] イオン性界面活性剤のゲル化に誘起されるマイクロメートルスケールの界面不安定性2014

    • Author(s)
      伊藤弘明、市川正敏
    • Organizer
      日本物理学会第69回年次大会
    • Place of Presentation
      東海大学
    • Year and Date
      2014-03-18
    • Related Report
      2013 Annual Research Report
  • [Presentation] Shape Fluctuation and Mechanics of Diseases2014

    • Author(s)
      Hiroaki Ito
    • Organizer
      Kick-Off Symposium SPIRITS program "Novel, In tegrated Clinicopathologic Diagnosis of Cancer Based on Physical Readouts"
    • Place of Presentation
      東京大学
    • Year and Date
      2014-03-08
    • Related Report
      2013 Annual Research Report
  • [Presentation] 油中水滴のサイズ依存的な油水界面透過ダイナミクス2014

    • Author(s)
      伊藤弘明
    • Organizer
      第4回京都若手ソフトマター研究会
    • Place of Presentation
      京都大学
    • Year and Date
      2014-03-07
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脂質単分子膜を用いた二分子膜小胞形成 : 速度論とサイズ依存性2013

    • Author(s)
      伊藤弘明、山中透、加藤翔, 濱田勉、高木昌宏、市川正敏、吉川研一
    • Organizer
      日本物理学会2013年秋季大会
    • Place of Presentation
      徳島大学
    • Year and Date
      2013-09-26
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dynamic penetration of a phospholipid-coated droplets across a planar monolayer at an oil/water interface2013

    • Author(s)
      H. Ito, T. Yamanaka, S. Kato, T. Hamada, M. Takagi, M. Ichikawa, and K. Yoshikawa
    • Organizer
      International Soft Matter Conference 2013
    • Place of Presentation
      ローマ、イタリア
    • Year and Date
      2013-09-16
    • Related Report
      2013 Annual Research Report
  • [Presentation] 油中水滴の油水界面透過ダイナミクス : 脂質二分子膜形成の速度過程モデリング2013

    • Author(s)
      伊藤弘明、市川正敏、吉川研一
    • Organizer
      西日本非平衡系ミニシンポジウム
    • Place of Presentation
      九州大学
    • Year and Date
      2013-05-31
    • Related Report
      2013 Annual Research Report
  • [Remarks] 京都大学理学研究科 市川研究室

    • URL

      http://www.chem.scphys.kyoto-u.ac.jp/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学理学研究科物理学第一教室、時空間秩序・生命物理研究室ホームページ

    • URL

      http://www.chem.scphys.kyoto-u.ac.jp

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2014-01-29   Modified: 2024-03-26  

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