• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

脳由来神経栄養因子(BDNF)の巨核球分化成熟過程に対する病態生理学的意義の解明

Research Project

Project/Area Number 13J04623
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Laboratory medicine
Research InstitutionNagoya University (2015)
University of Yamanashi (2013-2014)

Principal Investigator

田村 彰吾  名古屋大学, 医学(系)研究科(研究院), 助教

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsBDNF / 巨核球 / 脳由来神経栄養因子 (BDNF) / 脳由来神経栄養因子(BDNF)
Outline of Annual Research Achievements

これまでのin vitroの検討から、BDNFはマウス正常巨核球の自己増殖(clonal expansion)を促進することが明らかになった。そこで平成27年度はin vivoにおけるBDNFの巨核球・血小板造血への関与を検討した。血小板減少症モデルマウスにおいてBDNFは血小板数の回復を促進すると考え、本検討では抗マウスGPIb抗体カクテル投与による一過性血小板減少症モデルマウスを用いたBDNF投与実験を実施した。しかしながら、今回のモデルではBDNF投与マウスの有意な血小板数回復は認められなかった。本課題の今後の方針は、骨髄抑制モデルなど他の血小板減少症モデルにおけるBDNFの血小板・巨核球増殖促進作用の検討が挙げられる。
一方、申請者は本課題以外に血小板活性化受容体CLEC-2の血小板造血における役割に関する研究も手掛けた。CLEC-2の生体内リガンドである膜蛋白、ポドプラニンを発現した新規の間質細胞を同定し、同細胞のポドプラニンと巨核球のCLEC-2が結合して全く新しいニッシェが形成されることを見出した。このニッシェで、ポドプラニン刺激で巨核球の増殖が促進され、CLEC-2の刺激で間質細胞が産生するサイトカインが巨核球のproplatelet形成を促進するという、画期的な機序を解明した。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (18 results) (of which Int'l Joint Research: 4 results) Remarks (4 results)

  • [Journal Article] Podoplanin-positive periarteriolar stromal cells promote megakaryocyte growth and proplatelet formation in mice by CLEC-22016

    • Author(s)
      TAMURA Shogo, SUZUKI-INOUE Katsue, TSUKIJI Nagaharu, SHIRAI Toshiaki, SASAKI Tomoyuki, OSADA Makoto, SATOH Kaneo, OZAKI Yukio
    • Journal Title

      Blood

      Volume: 127 Issue: 13 Pages: 1701-1710

    • DOI

      10.1182/blood-2015-08-663708

    • NAID

      130007432250

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CD36の発現多様性 欠損症および健常者の発現分子数のバラつき2015

    • Author(s)
      田村彰吾、増田裕弥、松野一彦、尾崎由基男
    • Journal Title

      検査と技術

      Volume: 43 Pages: 186-188

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Journal Article] Diverse CD36 expression among Japanese population: defective CD36 mutations cause platelet and monocyte CD36 reductions in not only deficient but also normal phenotype subjects2015

    • Author(s)
      Masuda Y, Tamura S, Matsuno K, Nagasawa A, Hayasaka K, Shimizu C, Moriyama T
    • Journal Title

      Thrombosis Research

      Volume: 135 Issue: 5 Pages: 951-957

    • DOI

      10.1016/j.thromres.2015.03.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of soluble C-type lectin-like receptor 2 in human plasma2015

    • Author(s)
      Kazama F, Nakamura J, Osada M, Inoue O, Oosawa M, Tamura S, Tsukiji N, Aida K, Kawaguchi A, Takizawa S, Kaneshige A, Tanaka S, Suzuki-Inoue K, & Ozaki Y
    • Journal Title

      Platelets

      Volume: 26 Issue: 8 Pages: 711-719

    • DOI

      10.3109/09537104.2015.1021319

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laboratory and clinical features of abnormal macroenzymes found in human sera2014

    • Author(s)
      Moriyama T, Tamura S, Nakano K, Otsuka K, Shigemura M, Honma N
    • Journal Title

      BBA Proteins and Proteomics

      Volume: 1854 Issue: 6 Pages: 658-667

    • DOI

      10.1016/j.bbapap.2014.10.015

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a highly sensitive three—dimensional gel electrophoresis method for characterization of monoclonal protein heterogeneity2013

    • Author(s)
      Nakano K, Tamura S, Otuka K, Niizeki N, Shigemura M, Shimizu C, Matsuno K, Kobayashi S, Moriyama T
    • Journal Title

      Analytical Biochemistry

      Volume: 438 Pages: 117-123

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The basis examination of leukocyte-platelet aggregates with CD45 gating as a novel platelet activation marker2013

    • Author(s)
      Nagasawa A, Matsuno K, Tamura S, Hayasaka K, Shimizu C, Moriyama T
    • Journal Title

      International Journal of Laboratory Hematology

      Volume: 35 Pages: 534-541

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 事例で学ぶ免疫検査異常値への対応/1. 総論 : 免疫検査における非特異反応2013

    • Author(s)
      森山隆則、中野恵一、田村彰吾
    • Journal Title

      MEDICALTECHNOLOGY

      Volume: 41 Pages: 724-729

    • Related Report
      2013 Annual Research Report
  • [Presentation] CLEC-2 in megakaryopoiesis: CLEC-2/PDPN microenvironment facilitates expansion and maturation of megakaryocytes2015

    • Author(s)
      Tamura S, Suzuki-Inoue K, Tsukiji N, Shirai T, Sasaki T, Osada M, Satoh K, Ozaki Y
    • Organizer
      XXV congress of The international society on Thrombosis and Heamostasis (ISTH2015)
    • Place of Presentation
      Toronto
    • Year and Date
      2015-07-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The defective CD36 gene mutations cause a molecular expression diversity on platelets and monocytes even in non-deficient phenotype population2015

    • Author(s)
      Masuda Y, Tamura S, Matsuno K, Hayasaka K, Shimizu C, Moriyama T
    • Organizer
      XXV congress of The international society on Thrombosis and Heamostasis (ISTH2015)
    • Place of Presentation
      Toronto
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of expression system for biologically functional recombinant rhodocytin2015

    • Author(s)
      Sasaki T, Tamura S, Shirai T, Tsukiji N, Satoh K, Suzuki-Inoue K, Ozaki Y
    • Organizer
      XXV congress of The international society on Thrombosis and Heamostasis (ISTH2015)
    • Place of Presentation
      Toronto
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unexpected role of platelets in lung development depending on a platelet activation receptor, CLEC-22015

    • Author(s)
      Tsukiji N, Inoue O, Tamura S, Shirai T, Sasaki T, Satoh K, Suzuki-Inoue K, Ozaki Y
    • Organizer
      XXV congress of The international society on Thrombosis and Heamostasis (ISTH2015)
    • Place of Presentation
      Toronto
    • Year and Date
      2015-07-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CLEC-2/PDPN axisを介した巨核球造血微小環境2015

    • Author(s)
      田村彰吾、井上克枝、築地長治、白井俊光、佐々木知幸、佐藤金夫、尾崎由基男
    • Organizer
      第16回日本検査血液学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-07-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] CLEC-2/PDPN axisによる骨髄内微小環境の可能性:CLEC-2は巨核球造血を促進させる2015

    • Author(s)
      田村彰吾、井上克枝、白井俊光、築地長治、佐々木知幸、佐藤金夫、尾崎由基男
    • Organizer
      第37回日本血栓止血学会学術集会
    • Place of Presentation
      山梨
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 可溶性CLEC-2は血小板活性化に伴って生成される:可溶性GPVIとの比較検討2015

    • Author(s)
      井上修、長田誠、中村純也、風間文智、横道洋司、土肥智貴、金子誠、蔵野信、大澤満、田村彰吾、佐藤金夫、高野勝弘、宮内克己、代田浩之、矢富裕、井上克枝、尾崎由基男
    • Organizer
      第37回日本血栓止血学会学術集会
    • Place of Presentation
      山梨
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血小板は胚発生に必須である:血小板CLEC-2の機能解析2015

    • Author(s)
      築地長治、井上修、田村彰吾、白井俊光、佐々木知幸、佐藤金夫、井上克枝、尾崎由基男
    • Organizer
      第37回日本血栓止血学会学術集会
    • Place of Presentation
      山梨
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血小板活性化受容体CLEC-2結合蛇毒ロドサイチンの機能を保持したリコンビナント体発現系の構築2015

    • Author(s)
      佐々木知幸、田村彰吾、白井俊光、築地長治、佐藤金夫、井上克枝、尾崎由基男
    • Organizer
      第37回日本血栓止血学会学術集会
    • Place of Presentation
      山梨
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] A novel function of CLEC-2 for megakaryopoiesis: CLEC-2/PDPN niche promotes megakaryocyte expansion2015

    • Author(s)
      Shogo Tamura, Katsue Suzuki-Inoue, Toshiaki Shirai, Nagaharu Tsukiji, Kaneo Satoh, Yukio Ozaki
    • Organizer
      Joint International Symposium on TGF-b Family and Cancer Signal Network in Tumor Microenvironment
    • Place of Presentation
      International congress centror EPOCHAL TSUKUBA (Tsukuba, Ibaraki)
    • Year and Date
      2015-01-12 – 2015-01-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血小板・単球におけるCD36 分子発現の多様性と遺伝子変異との関連2014

    • Author(s)
      増田 裕弥,田村 彰吾,松野 一彦,早坂 光司,渋谷 斉,清水 力,森山 隆則
    • Organizer
      第61回日本臨床検査医学会学術集会
    • Place of Presentation
      福岡国際会議場 (福岡県福岡市)
    • Year and Date
      2014-11-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 巨核球造血におけるCLEC-2 の病態生理学的作用の解析2014

    • Author(s)
      田村 彰吾,井上 克枝,白井 俊光,築地 長治,佐藤 金夫,尾崎由基男
    • Organizer
      第61回日本臨床検査医学会学術集会
    • Place of Presentation
      福岡国際会議場 (福岡県福岡市)
    • Year and Date
      2014-11-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Vacuolating cytotoxin A (VacA) of Helicobactor Pylori binds to Multimerin12014

    • Author(s)
      Kaneo Satoh, Toshiya Hirayama, Masato Ohta, Shogo Tamura, Yuko Ozaki
    • Organizer
      The 3rd World Congress on Controversies in Hematology
    • Place of Presentation
      The Marmara Taksim Hotel (Instanbul, Turkey)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血小板におけるCD36発現分子数の低下と活性化による変化2014

    • Author(s)
      増田裕弥、田村彰吾、長澤あゆみ、松野一彦、進藤由衣香、早坂光司、渋谷 斉、清水 力、森山隆則
    • Organizer
      第15回日本検査血液学会学術集会
    • Place of Presentation
      仙台国際センター (宮城県仙台市)
    • Year and Date
      2014-07-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血小板におけるCD36分子発現量の多様性と一塩基多型(C268T)の関連2014

    • Author(s)
      増田裕弥、田村彰吾、長澤あゆみ、松野一彦、進藤由衣香、早坂光司、渋谷 斉、清水 力、尾崎由基男、森山隆則
    • Organizer
      第36回日本血栓止血学会学術集会
    • Place of Presentation
      大阪国際交流センター (大阪府大阪市)
    • Year and Date
      2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Unexpected role of blood platelets in lung development depending on a novel platelet activation receptor, CLEC-2 (血小板が正常な肺発生を可能にする –新規血小板活性化受容体CLEC-2の機能解析-)2014

    • Author(s)
      Nagaharu Tsukiji, Osamu Inoue, Shogo Tamura, Toshiaki Shirai, Katsue Suzuki-Inoue, Yukio Ozaki
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      WINC AICHI (愛知県名古屋市)
    • Year and Date
      2014-05-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血小板CD36分子数の多様性におけるCD36遺伝子の一塩基多型の意義2013

    • Author(s)
      田村彰吾、松野一彦、長澤あゆみ、増田裕弥, 早坂光司、清水力、山本正雅、尾崎由基男、森山隆則
    • Organizer
      第35回日本血栓止血学会学術集会
    • Place of Presentation
      山形国際ホテル(山形県山形市)
    • Year and Date
      2013-05-31
    • Related Report
      2013 Annual Research Report
  • [Presentation] BDNF acts as an autocrine cell proliferation factor in TPO stimulated megakaryocytic cell line2013

    • Author(s)
      Shogo Tamura, Takanori Moriyama, Kazuhiko Matsuno, Katsue Suzuki-Inoue, Yukio Ozaki
    • Organizer
      XV Congress of the International Society of Thrombosis and Haemostasis
    • Place of Presentation
      Amsterdam RAI (Amsterdam, Netherlands)
    • Related Report
      2013 Annual Research Report
  • [Remarks] 山梨大学医学部臨床検査医学講座 ホームページ

    • URL

      http://www.med.yamanashi.ac.jp/clinical/clin0lab/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 名古屋大学大学院医学系研究科 医療技術学専攻 病態解析学講座 分子病態解析学部門 血液・遺伝子研究室

    • URL

      http://square.umin.ac.jp/bloodlab/Hematol_%26_Gene_Res_Lab/Welcome.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 山梨大学・臨床検査医学講座

    • URL

      http://www.med.yamanashi.ac.jp/clinical/clin0lab/

    • Related Report
      2014 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.yamanashi.ac.jp/clinical/clin0lab/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2014-01-29   Modified: 2024-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi