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The Single Molecular Design and Systemic Assembly of Protein Function

Research Project

Project/Area Number 14002008
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionWaseda University

Principal Investigator

ISHIWATA Shinichi  Waseda University, Faculty of Science and Engineering, Professor, 理工学術院, 教授 (10130866)

Co-Investigator(Kenkyū-buntansha) FUNATSU Takashi  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Professor, 薬学研究科(研究院), 教授 (00190124)
Project Period (FY) 2002 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥585,000,000 (Direct Cost: ¥450,000,000、Indirect Cost: ¥135,000,000)
Fiscal Year 2006: ¥119,600,000 (Direct Cost: ¥92,000,000、Indirect Cost: ¥27,600,000)
Fiscal Year 2005: ¥118,300,000 (Direct Cost: ¥91,000,000、Indirect Cost: ¥27,300,000)
Fiscal Year 2004: ¥119,600,000 (Direct Cost: ¥92,000,000、Indirect Cost: ¥27,600,000)
Fiscal Year 2003: ¥110,500,000 (Direct Cost: ¥85,000,000、Indirect Cost: ¥25,500,000)
Fiscal Year 2002: ¥117,000,000 (Direct Cost: ¥90,000,000、Indirect Cost: ¥27,000,000)
KeywordsBiomolecular motor / Bionanogauge / Optical Tweezers / SPOC / Microthermometer / Photon Counting Histogram / Actin / Mitotic Spindle / ミクロ温度計測 / 1分子蛍光イメージング / ナノバイオロジー / ナノ筋収縮系 / シャペロニン / 筋収縮系 / シャペロン
Research Abstract

Biological functions are inherently hierarchical. We planned to investigate the underlying mechanism of biological functions from the single-molecular level to the level of supramolecular assemblies, focusing on the mechanism characteristic of each level of hierarchy. Our study was focused primarily on a biological motility system starting from interactions between single-molecular motors and track polymers, to whole ensembles of such interactions (nanomuscle, myofibrils and fibers), and on to single cells and whole tissues (the heart). We also aimed to develop a technique that will allow the use of the cytoskeleton as a "bionanogauge". The main results of this project are summarized as follows: 1) Microscopic measurements of unbinding force of actin-myosin V or VI complex under various concentrations of ADP showed that the apparent binding affinity of ADP depends on the loading direction. Based on a simple model analysis, we could determine the load dependence of the attachment and detachment rate constants of ADP to actomyosin complex, which helped understanding of the molecular mechanism of myosin motility. 2) We have demonstrated that the fluorescence intensity of rhodamine-labeled single actin filaments changes depending on the applied load, implying that the local structure of actin is distorted. 3) We presented evidence for that the changes of filament lattice are responsible for the mechanism of SPOC of myofibrils. 4) We have succeeded in identifying the amino acids of tubulin that are responsible for the strong binding of kinesin using genetically engineered tubulin molecules. 5) Using a micro-thermometer, we could find that the application of ionomycin induced the temperature increase by 1℃ in single HeLa cells, which is attributable to the Ca^<2+> uptake. 6) Using the Photon Counting Histogram technique, we showed that actin filaments consist of not only helical polymers but also linear polymers as theoretically predicted by F. Oosawa and his associates.

Report

(6 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (113 results)

All 2007 2006 2005 2004 Other

All Journal Article (81 results) Book (1 results) Patent(Industrial Property Rights) (3 results) Publications (28 results)

  • [Journal Article] Microscopic detection of thermogenesis in a single HeLa cell.2007

    • Author(s)
      Suzuki, M., Tseeb, V., Oyama, K., Ishiwata, S.
    • Journal Title

      Biophys. J. 92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Regulation of muscle contraction by Ca2+ and ADP : Focusing on the auto-oscillation (SPOC).2007

    • Author(s)
      Ishiwata, S., Shimamoto, Y., Suzuki, M., Sasaki, D.
    • Journal Title

      Adv. Exp. Med. Biol. 592

      Pages: 341-358

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Label-free DNA sensors using ultrasensitive diamond field-effect transistors in solution.2007

    • Author(s)
      Song K.S., et al.
    • Journal Title

      Phys Rev E Stat Nonlin Soft Matter Phys. 74

      Pages: 41919-41919

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Size distribution of linear and helical polymers in actin solution analyzed by photon counting histogram.2007

    • Author(s)
      Terada, N., Shimozawa, T., Ishiwata, S., Funatsu, T.
    • Journal Title

      Biophys. J. 92

      Pages: 2162-2171

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Size distribution of linear and helical polymers in actin solution analyzed by photon counting histogram2007

    • Author(s)
      Terada, N., Shimozawa, T., Ishiwata, S., Funatsu, T.
    • Journal Title

      Biophys. J. 92

      Pages: 2162-2171

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of muscle contraction by Ca2+ and ADP : Focusing on the auto-oscillation (SPOC)2007

    • Author(s)
      Ishiwata, S., Shimamoto, Y., Suzuki, M., Sasaki, D.
    • Journal Title

      Adv. Exp. Med. Biol. 592

      Pages: 341-358

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Microscopic detection of thermogenesis in a single HeLa cell2007

    • Author(s)
      Suzuki, M., Tseeb, V., Oyama, K., Ishiwata, S.
    • Journal Title

      Biophys. J. 92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Myocardial sarcomeres spontaneously oscillate with the period of heartbeat under physiological conditions.2006

    • Author(s)
      Sasaki, D., Fukuda, N., Ishiwata, S.
    • Journal Title

      Biochem. Biophys. Res. Commun. 343

      Pages: 1146-1152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Temperature change does not affect force between regulated actin filaments and HMM in single molecule experiments.2006

    • Author(s)
      Kawai, M., Kido, T., Vogel, M., Fink, R.H.A., Ishiwata, S.
    • Journal Title

      J. Physiol. (Lond.) 574・3

      Pages: 877-887

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Use of thin-filament reconstituted muscle fibres to probe the mechanism of force generation.2006

    • Author(s)
      Kawai, M., Ishiwata, S.
    • Journal Title

      J. Muscle Res. Cell Motil. 27

      Pages: 455-468

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Modulation of hippocampal neuron survival by thrombin and factor Xa.2006

    • Author(s)
      Gorbacheva, L.R., Storozhevykh, T.P., et al.
    • Journal Title

      Biochemistry(Moscow) 71

      Pages: 1082-1089

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification of a strong binding site for kinesin on the microtubule using mutant analysis of tubulin.2006

    • Author(s)
      Uchimura, S., Oguchi, Y., et al.
    • Journal Title

      EMBO J. 25

      Pages: 5932-5941

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Single-molecular analysis of the binding state of myosin V and actin.2006

    • Author(s)
      Dguchi, Y., et al.
    • Journal Title

      J. Physics : Conference Series 31

      Pages: 239-240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Synthesis of the isotope-labeled derivatization reagent for carboxylic acids, 7-(N, N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2, 1, 3-benzoxadiazo le (d6) (DBD-PZ-NH2 (D)), and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high-performance liquid chromatography/mass spectrometry.2006

    • Author(s)
      Tsukamoto Y, T. et al.
    • Journal Title

      Biomedical Chromatography 20

      Pages: 358-364

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Catecholamine analysis with microcolumn LC-peroxyoxalate chemiluminescence reaction detection.2006

    • Author(s)
      Tsunoda, M. et al.
    • Journal Title

      Clin. Chim. Acta. 366

      Pages: 168-173

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] DNA micropatterning of polycrystalline diamond via one-step direct amination.2006

    • Author(s)
      Zhang, G.J.et al.
    • Journal Title

      Langmuir 22

      Pages: 3728-3734

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A further study on the combined use of internal standard and isotope-labeled derivatization reagent for expansion of linear dynamic ranges in liquid chromatography-electrospray mass spectrometry.2006

    • Author(s)
      Tsukamoto, Y. et al.
    • Journal Title

      Biomed. Chromatogr. 20

      Pages: 1049-1055

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Imaging of single mRNA molecules moving within a living cell nucleus.2006

    • Author(s)
      Tadakuma, H., et al.
    • Journal Title

      Bichem Biophys Res Commun. 344

      Pages: 772-779

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Suppression of thiol exchange reaction in the determination of reduced-form thiols by high-performance liquid chromatography with fluorescence detection after derivatization with fluorogenic benzofurazan reagent, 7-fluoro-2, 1, 3-benzoxadiazole-4-sulfonate and 4-aminosulfonyl-7-fluoro-2, 1, 3- benzoxadiazole.2006

    • Author(s)
      Santa, T., et al.
    • Journal Title

      Biomed Chromatogr. 20

      Pages: 656-661

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Determination of N^G, N^G-dimethyl-l-arginine in rat plasma and dimethylarginine dimethylaminohydrolase activity in rat kidney using a monolithic silica column.2006

    • Author(s)
      Nonaka, S., et al.
    • Journal Title

      J. Chromatogr. B 843

      Pages: 170-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cellular responses of the ciliate, Tetrahymena thermophila, to far infrared irradiation.2006

    • Author(s)
      Shiurba, R., et al.
    • Journal Title

      Photochem. Photobiol. Sci. 5

      Pages: 799-807

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Localization of Prefoldin Interaction Sites in the Hyperthermophilic Group II Chaperonin and Correlations between Binding Rate and Protein Transfer Rate.2006

    • Author(s)
      Zako, T., et al.
    • Journal Title

      J. Mol. Biol. 364

      Pages: 110-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel.2006

    • Author(s)
      Arakawa, T., et al.
    • Journal Title

      Meas. Sci. Techn. 17

      Pages: 3141-3146

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 1分子イメージングでタンパク質の機能を探る2006

    • Author(s)
      船津高志
    • Journal Title

      日本レーザー医学会誌 26

      Pages: 327-332

    • NAID

      10017277629

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 現場で役立つバイオイメージング 第1回全反射蛍光顕微鏡による1分子イメージング(基礎編)2006

    • Author(s)
      船津高志
    • Journal Title

      バイオニクス 4月号

      Pages: 76-83

    • NAID

      40007178972

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 現場で役立つバイオイメージング 第2回全反射蛍光顕微鏡による1分子イメージング(応用編)2006

    • Author(s)
      船津高志
    • Journal Title

      バイオニクス 5月号

      Pages: 64-70

    • NAID

      40007216165

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] MRNA核内運動の1分子イメージング2006

    • Author(s)
      船津高志, 谷時雄
    • Journal Title

      ファルマシア 42・8

      Pages: 803-806

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] マイクロ・ナノデバイスを用いた生体分子の1分子計測と分離操作2006

    • Author(s)
      船津高志
    • Journal Title

      Drug Delivery System 21・6

    • NAID

      10018443478

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Temperature change does not affect force between regulated actin filaments and HMM in single molecule experiments2006

    • Author(s)
      Kawai, M., Kido, T., Vogel, M., Fink, R.H.A., Ishiwata, S.
    • Journal Title

      J. Physiol. (Lond.) 574. 3

      Pages: 877-887

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Use of thin-filament reconstituted muscle fibres to probe the mechanism of force generation2006

    • Author(s)
      Kawai, M., Ishiwata, S.
    • Journal Title

      J. Muscle Res. Cell Motil. 27

      Pages: 455-468

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modulation of hippocampal neuron survival by thrombin and factor Xa2006

    • Author(s)
      Gorbacheva, L.R., Storozhevykh, T.P., Pinelis, V.G., Ishiwata, S., Strukova, S.M.
    • Journal Title

      Biochemistry (Moscow) 71

      Pages: 1082-1089

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of a strong binding site for kinesin on the microtubule using mutant analysis of tubulin2006

    • Author(s)
      Uchimura, S., Oguchi, Y., Katsuki, M., Usui, T., Osada, H., Nikawa, J., Ishiwata, S., Muto, E.
    • Journal Title

      EMBO J. 25

      Pages: 5932-5941

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Synthesis of the isotope-labeled derivatization reagent for carboxylic acids, 7-(N, N-dimethylaminosulfony1)-4-(aminoethyl)piperazino-2,1,3-benzoxadiazole (d_6) (DBD-PZ-NH_2 (D)), and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high-performance liquid chromatography/mass spectrometry2006

    • Author(s)
      Tsukamoto Y, T.Santa, H.Yoshida, H.Miyano, T.Fukushima, K.Hirayama, K.Imai, T.Funatsu.
    • Journal Title

      Biomedical Chromatography 20

      Pages: 358-364

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Catecholamine analysis with microcolumn LC-peroxyoxalate chemiluminescence reaction detection2006

    • Author(s)
      Tsunoda, M., M.Nagayama, T.Funatsu, S.Hosoda, K.Imai.
    • Journal Title

      Clin. Chim. Acta. 366

      Pages: 168-173

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] DNA micropatterning on polycrystalline diamond via one-step direct amination2006

    • Author(s)
      Zhang, G.J., K.S.Song, Y.Nakamura, T.Ueno, T.Funatsu, I.Ohdomari, H.Kawarada.
    • Journal Title

      Langmuir 22

      Pages: 3728-3734

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A further study on the combined use of internal standard and isotope-labeled derivatization reagent for expansion of linear dynamic ranges in liquid chromatography-electrospray mass spectrometry2006

    • Author(s)
      Tsukamoto, Y., T.Santa, H.Yoshida, H.Miyano, T.Fukushima, K.Hirayama, K.Imai, T.Funatsu.
    • Journal Title

      Biomed. Chromatogr. 20

      Pages: 1049-1055

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Imaging of single mRNA molecules moving within a living cell nucleus2006

    • Author(s)
      Tadakuma, H., Y.Ishihama, T.Shibuya, T.Tani, T.Funatsu.
    • Journal Title

      Biochem Biophys Res Commun. 344

      Pages: 772-779

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Suppression of thiol exchange reaction in the determination of reduced-form thiols by high-performance liquid chromatography with fluorescence detection after derivatization with fluorogenic benzofurazan reagent, 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate and 4-aminosulfony1-7-fluoro-2,1,3- benzoxadiazole2006

    • Author(s)
      Santa, T., C.Aoyama, T.Fukushima, K.Imai, T.Funatsu.
    • Journal Title

      Biomed Chromatogr. 20

      Pages: 656-661

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Determination of NG, NG-dimethy1-1-arginine in rat plasma and dimethylarginine dimethylaminohydrolase activity in rat kidney using a monolithic silica column2006

    • Author(s)
      Nonaka, S., M.Tsunoda, C.Aoyama, T.Funatsu.
    • Journal Title

      J. Chromatogr. B. 843

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Higashinakagawa. Cellular responses of the ciliate, Tetrahymena thermophila, to far infrared irradiation2006

    • Author(s)
      Shiurba, R., T.Hirabayashi, M.Masuda, A.Kawamura, Y.Komoike, W.Klitz, K.Kinowaki, T.Funatsu, S.Kondo, S.Kiyokawa, T.Sugai, K.Kawamura, H.Namiki, T.
    • Journal Title

      Photochem. Photobiol. Sci. 5

      Pages: 799-807

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Localization of Prefoldin Interaction Sites in the Hyperthermophilic Group II Chaperonin and Correlations between Binding Rate and Protein Transfer Rate2006

    • Author(s)
      Zako, T., Y.Murase, R.Iizuka, T.Yoshida, T.Kanzaki, N.Ide, M.Maeda, T.Funatsu, M.Yohda.
    • Journal Title

      J. Mol. Biol. 364

      Pages: 110-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel2006

    • Author(s)
      Arakawa, T., Y.Shirasaki, T.Izumi, T.Aoki, H.Sugino, T.Funatsu, S.Shoji.
    • Journal Title

      Meas. Sci. Techn. 17

      Pages: 3141-3146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Single-molecular analysis of the binding state of myosin V and actin.2006

    • Author(s)
      Oguchi, Y., et al.
    • Journal Title

      J. Physics : Conference Series 31

      Pages: 239-240

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Synthesis of the isotope-labeled derivatization reagent for carboxylic acids, 7-(N,N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2,1,3-benzoxadiazole (d6) (DBD-PZ-NH2 (D)), and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high-performance liquid chromatography/mass spectrometry.2006

    • Author(s)
      Tsukamoto Y, T. et al.
    • Journal Title

      Biomedical Chromatography 20

      Pages: 358-364

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Suppression of thiol exchange reaction in the determination of reduced-form thiols by high-performance liquid chromatography with fluorescence detection after derivatization with fluorogenic benzofurazan reagent, 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate and 4-aminosulfonyl-7-fluoro-2,1,3- benzoxadiazole.2006

    • Author(s)
      Santa, T., et al.
    • Journal Title

      Biomed Chromatogr. 20

      Pages: 656-661

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Determination of N^G,N^G-dimethyl-1-arginine in rat plasma and dimethylarginine dimethylaminohydrolase activity in rat kidney using a monolithic silica column.2006

    • Author(s)
      Nonaka, S., et al.
    • Journal Title

      J. Chromatogr. B 843

      Pages: 170-174

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells : Nuclear mRNA export is coupled to ongoing gene transcription.2006

    • Author(s)
      Tokunaga, K., Shibuya, T., Ishihama, Y., Tadakuma, H., et al.
    • Journal Title

      Genes to Cells. 11

      Pages: 305-317

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A new muscle contractile system composedof a thick filament lattice and a single actin filament.2005

    • Author(s)
      Suzuki, M., Fujita, H., Ishiwata, S.
    • Journal Title

      Biophys.J. 89(1)

      Pages: 321-328

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Auto-oscillations of skinned myocardium correlating with heartbeat.2005

    • Author(s)
      Sasaki, D., Fujita, H., Fukuda, N., Kurihara, S., Ishiwata, S.
    • Journal Title

      J.Muscle Res.Cell Motil. 26(2)

      Pages: 93-101

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Synthesis of benzofurazan derivatization regents for carboxylic and acids and its application to analysis of fatty acids in rat plasma by high-performance liquid chromatography/electrospray ionization mass spectrometry.2005

    • Author(s)
      Tsukamoto, Y., Santa, T., Saimaru, H., Imai, K., Funatsu, T.
    • Journal Title

      Biomed.Chromatogy. 19

      Pages: 802-808

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hybridization of deoxyribonucleic acid immobilization of green fluorescent protein on nanostructured porganosilane templates.2005

    • Author(s)
      Tanii, T., Hosaka, T., Miyake, T., Kanari, Y., Zhang, G.-J., et al.
    • Journal Title

      Jpn.J.App.Phys. 44

      Pages: 5851-5855

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nanoscale patterning of protein using electron beam lithography of organosilane self-assembled monolayers.2005

    • Author(s)
      Zhang, G.-J., Tanii, T., Zako, T., Hosaka, T., Miyake, T., et al.
    • Journal Title

      Small 1

      Pages: 833-837

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Facilitated release of substrate protein from prefoldin by chaperonin.2005

    • Author(s)
      Zako, T., Iizuka, R., Okochi, M., Nomura, T., Ueno, T., Tadakuma, H., Yohda, M., Funatsu, T.
    • Journal Title

      FEBS Lett. 579

      Pages: 3718-3724

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Determination of methylated arginises by column-switching high-performance liquid chromatography-fluorescence detection.2005

    • Author(s)
      Tsunoda, M., Nonaka, S., Funatsu, T.
    • Journal Title

      Analyst 130

      Pages: 1410-1413

    • Related Report
      2005 Annual Research Report
  • [Journal Article] On-chip cell sorting system using laser-induced heating of a thermo-reversible gelation polymer to control flow.2005

    • Author(s)
      Shirasaki, Y., Tanaka, J., Makazu, H., Tashiro, K., Shoji, S.et al.
    • Journal Title

      Anal.Chem. 78

      Pages: 695-701

    • Related Report
      2005 Annual Research Report
  • [Journal Article] High-performance liquid chromatographic assay of NG-monomethyl-L-arginine, NG, NG-dimethyl-L-arginine, and NG.NG'-dimethyl-L-arginine using 4-fluoro-7-nitro-2, 1, 3-benzoxadiazole as a fluorescent reagent.2005

    • Author(s)
      Nonaka, S., Tsunoda, M., Imai, K.Funatsu, T.
    • Journal Title

      J.Chromatogr.A (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rapid assay for catechol-0-methyltransferase activity by high-performance liquid chromatography-fluorescence detection.2005

    • Author(s)
      Hirao, Y., Tsunoda, M.Funatsu, T., Imai, K.
    • Journal Title

      J.Chromatogr.B (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A 1480/1064 nm dual wavelength photo-thermal etching system for non-contact three-dimensional microstructure generation into agar microculture chip.2004

    • Author(s)
      Hattori, A., Moriguchi, H., Ishiwata, S., Yasuda, K.
    • Journal Title

      Sensors & Actuators B. 100

      Pages: 467-474

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel method of thermal activation and temperature measurement in the microscopic region around single living cells.2004

    • Author(s)
      Zeeb, V., Suzzki, M., Ishiwata, S.
    • Journal Title

      J.Neurosci.Meth. 139

      Pages: 69-77

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mechanochemical coupling of two substeps in a single myosin V motor.2004

    • Author(s)
      Uemura, S., Higuchi, H., Olivares, A.O.et al.
    • Journal Title

      Nature Struct.Mol.Biol. 11

      Pages: 877-883

    • Related Report
      2004 Annual Research Report
  • [Journal Article] GroEL mediates protein folding with a two successive timer mechanism.2004

    • Author(s)
      Ueno, T., Taguchi, H., Tadakuma, H., Yoshida, M., Funatsu, T.
    • Journal Title

      Molecular Cell 14

      Pages: 423-434

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Kinetics and binding sites for interaction of prefoldin with group II chaperonin : contiguous non-native substrate and chaperonin binding sites in archaeal prefoldin.2004

    • Author(s)
      Okochi, M., Nomura, T., Zako, T., Iizuka, H., Ueda, H., Funatsu, T.et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 31788-31795

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Preferential immobilization of biomolecules on silicon microstructure array by means of electron beam lithography on organosilane self-assembled monolayer resist.2004

    • Author(s)
      Tani i, T., Hosaka, T., Miyake, T., Zhang, G., Zako, T., Funatsu, T.et al.
    • Journal Title

      Appl.Surf.Sci. 234

      Pages: 102-106

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Micropatterning oligonucletides on single crystal diamond surface by photolithography.2004

    • Author(s)
      Zang, G., Umezawa, H., Hata, H., Zako.T., Funatsu, T.et al.
    • Journal Title

      Jpn.J.Appl.Phys. 44

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel.

    • Author(s)
      Arakawa, T., et al.
    • Journal Title

      Sensors and Actuators A : Physical (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Determination of potent antisense oligonucleotides in vitro by semi-empirical rules.

    • Author(s)
      Yanagihara, N. H., et al.
    • Journal Title

      J. Biosci. Bioeng (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] On-chip Cell Sorting System Using Thermoreversible Gelation Polymer.

    • Author(s)
      Shirasaki, Y., et al.
    • Journal Title

      IEEE J. Selected Topics in Quantum Electronics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Suppression of COMT activity through blunting α 2-adrenoceptor can explain hypertension in Dahl Salt-Sensitive rats.

    • Author(s)
      Hirano, Y., et al.
    • Journal Title

      Hypertens. Res. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Label-free DNA sensors using ultrasensitive diamond field-effect transistors in solution

    • Author(s)
      Song K.S., G.J.Zhang, Y.Nakamura, K.Furukawa, T.Hiraki, J.H.Yang, T.Funatsu, I.Ohdomari, H.Kawarada
    • Journal Title

      Phys Rev E Stat Nonlin Soft Matter Phys. 74

      Pages: 41919-41919

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel

    • Author(s)
      Arakawa, T.Y.Shirasaki, T.Aoki, T.Funatsu, S.Shoji
    • Journal Title

      Sensors and Actuators A : Physical (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Determination of potent antisense oligonucleotides in vitro by semi-empirical rules

    • Author(s)
      Yanagihara, N.H.Tadakuma, Y.Ishihama, K.Okabe, T.Funatsu
    • Journal Title

      J. Biosci. Bioeng. (in press)

    • NAID

      110006271249

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] On-chip Cell Sorting System Using Thermoreversible Gelation Polymer

    • Author(s)
      Shirasaki, Y., H.Sugino, M.Tatsuoka, J.Mizuno, S.Shoji, T.Funatsu
    • Journal Title

      IEEE J. Selected Topics in Quantum Electronics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Suppression of COMT activity through blunting α 2-adrenoceptor can explain hypertension in Dahl Salt-Sensitive rats

    • Author(s)
      Hirano, Y., M.Tsunoda, T.Shimosawa, H.Matsui, T.Fujita, T.Funatsu
    • Journal Title

      Hypertens. Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel.

    • Author(s)
      Arakawa, T., et al.
    • Journal Title

      Sensors and Actuators A : Physical (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Determination of potent antisense oligonucleotides in vitro by semi-empirical rules.

    • Author(s)
      Yanagihara, N. H., et al.
    • Journal Title

      J. Biosci. Bioeng (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] On-chip Cell Sorting System Using Thermoreversible Gelation Polymer.

    • Author(s)
      Shirasaki, Y., et al.
    • Journal Title

      IEEE J. Selected Topics in Quantum Electronics (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Suppression of COMT activity through blunting □2-adrenoceptor can explain hypertension in Dahl Salt-Sensitive rats.

    • Author(s)
      Hirano, Y., et al.
    • Journal Title

      Hypertens. Res. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Synthesis of the isotope-labeled derivatization reagent for carboxylic acids, 7-(N,N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2,1,3-…

    • Author(s)
      Tsukamoto, Y., Santa, T., Yoshida, H., Miyano, H., et al.
    • Journal Title

      Biomedical Chromatography (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] DNA micropatterning on polycrystalline deamond via one-step direct amination.

    • Author(s)
      Zhang, G.J., Song, K.S., Nakamura, Y., Ueno, T., Funatsu, T.et al.
    • Journal Title

      Langmuir (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Catecholamine analysis with microcolumn LC-peroxyoxalate chemiluminescence reaction detection.

    • Author(s)
      Tsunoda, M., Nagayama, M., Funatsu, T., Hosoda, S., Imai, K.
    • Journal Title

      Clin.Chim.Acta. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Analysis of Nuclear Microenvironments by Translational Diffusion of GFP Using Fluorescence Correlation Spectroscopy.

    • Author(s)
      Terada, N., Tadakuma, H., Ishihama, Y., Yamagishi, M., Zako, T., Funatsu, T.
    • Journal Title

      Bioimages. (in press)

    • NAID

      10020936956

    • Related Report
      2005 Annual Research Report
  • [Book] MEMS 20062006

    • Author(s)
      Arakawa T., Izumi, T., Teraguchi, Aoki, T.Shirasaki, Y., Sugino, H., et al.
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 微小開口膜,生体分子間相互作用解析方法及びその装置2006

    • Inventor(s)
      谷井孝至他
    • Industrial Property Rights Holder
      早稲田大学
    • Industrial Property Number
      2006-015559
    • Filing Date
      2006-01-24
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 分子振動計測法、分子振動計測装置、試料調製用キット及び分子振動計測システム2005

    • Inventor(s)
      船津高志他
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Number
      2005-219539
    • Filing Date
      2005-07-28
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 微粒子分別マイクロシステムおよび微粒子分別方法2005

    • Inventor(s)
      荒川貴博他
    • Industrial Property Rights Holder
      早稲田大学
    • Industrial Property Number
      2005-345393
    • Filing Date
      2005-07-28
    • Related Report
      2005 Annual Research Report
  • [Publications] Kawaguchi, K., Uemura, S., Ishiwata, S.: "Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics."Biophys.J.. 84. 1103-1113 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Uemura, S., Ishiwata, S.: "Loading direction regulates the affinity of ADP for kinesin."Nature Structural Biology. 10. 308-311 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujita, H., Lu, X., Suzuki, M., Ishiwata, S., Kawai, M.: "The effect of tropomyosin on isometric tension and the elementary steps of the cross-bridge cycle."J.Physiol. (in press)(未定). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hattori, A., Moriguchi, H., Ishiwata, S., Yasuda, K.: "A 1480/1064 nm dual wavelength photo-thermal etching system for non-contactthree-dimensional microstructure generation into agar microculture chip."Sensors & Actuators B.. (in press)(未定). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 上野太郎, 多田隈尚史, 船津高志: "シャペロニン機能の1分子蛍光イメージング"センサマイクロマシーン準部門誌. 123・4. 107-111 (2003)

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  • [Publications] 船津高志: "単一分子計測とバイオフォトニクス"応用物理. 72・6. 727-730 (2003)

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  • [Publications] 多田隈尚史, 船津高志, 谷時雄: "MRNA核外輸送の可視化"蛋白質核酸酵素. 48. 421-429 (2003)

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      2003 Annual Research Report
  • [Publications] Zhang, G., Tanii, T., Zako, T., Funatsu, T., Ohdomari, I.: "The immobilization of DNA on microstructured patterns fabricated by maskless lithography."Sensors and Actuators B.. 97. 243-248 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhang, G.-J., Tanii, T., Funatsu, T., Ohdomari, I.: "Patterning of DNA nanostructures on silicon surface by electron beam lithography of self-assembled monolayer."Chem Comm.. (in press)(未定). (2004)

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      2003 Annual Research Report
  • [Publications] 島本勇太, 鈴木団, 石渡信一: "ナノ・IT時代の分子機能材料と素子開発"NTS. 574-580 (2004)

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      2003 Annual Research Report
  • [Publications] Suzuki, M., Ishiwata, S.: "In Reflexive polymers and hydrogels : Understanding and designing fast-responsive polymeric systems"CRC Press LLC.(in press). 33-47 (2004)

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      2003 Annual Research Report
  • [Publications] Kanai, M., Uchida, D., Sugiura, S., Shirasaki, Y.et al.: "PDMS microfluidics devices with PTFE passivated cannels."Micro Total Analysis System.. 429-432 (2003)

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      2003 Annual Research Report
  • [Publications] Shoji, S., Tashiro, K., Sekiguchi, T., Sato, H.et al.: "Lab-on-a-Chip. Miniaturized Systems for (Bio) Chemical Analysis and Systhesis."Elsevier B.V.. 205-214 (2003)

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      2003 Annual Research Report
  • [Publications] 多田隈尚史, 船津高志: "ナノテクノロジーハンドブック(I〜IV)"オーム社. 94-99 (2003)

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      2003 Annual Research Report
  • [Publications] 船津高志: "ナノテクノロジー基礎シリーズ バイオナノテクノロジー"オーム社. 222 (2003)

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  • [Publications] 多田隈尚史, 座古保, 船津高志: "ナノバイオテクノロジー最前線"シーエムシー出版. 371-379 (2003)

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  • [Publications] 上野太郎, 多田隈尚史, 船津高志: "細胞における蛋白質の一生"共立出版(印刷中). (2004)

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  • [Publications] Uemura, S. et al.: "Kinesin-microtubule binding depends on both nucleotide state and loading direction"Proc. Natl. Acad. Sci. USA. 99. 5977-5981 (2002)

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  • [Publications] Ali, M.Y.et al.: "Myosin V is a left-handed spiral motor on the right-handed actin helix"Nature Struct. Biol.. 9. 464-467 (2002)

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  • [Publications] Fujiwara, I. et al.: "Visualization and force measurement of branching by Arp2/3 complex and N-WASP in actin filament"Biochem. Biophys. Res. Commun.. 293. 1550-1555 (2002)

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  • [Publications] Fujita, H. et al.: "Regulatory roles of light chain 2 in MgADP-induced contraction"J. Physiol.. 542. 221-229 (2002)

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      2002 Annual Research Report
  • [Publications] 川口憲治, 上村想太郎, 石渡信一: "キネシン分子モーターの仕組み"生物物理. 42. 156-162 (2002)

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  • [Publications] Fujiwara, I. et al.: "Direct observation of polymerization-depolymerization dynamics of single actin filaments"Nature Cell Biol.. 4. 666-673 (2002)

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      2002 Annual Research Report
  • [Publications] Funatsu, T. et al.: "Rapid and sensitive detection method of a bacterium using GFP reporter phage"Microbiol. Immuno.. 46. 365-369 (2002)

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      2002 Annual Research Report
  • [Publications] 船津高志: "1分子蛍光イメージング技術による生命現象の解析"可視化情報学会誌. 22. 18-21 (2002)

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      2002 Annual Research Report
  • [Publications] 座古保, 船津高志: "生体機能を1分子レベルで探る-1分子蛍光イメージング"分光研究. 51. 3-14 (2002)

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  • [Publications] Shirasaki, Y. et al.: "Micro Total Analysis System Vol.2"Kluwer Academic Publishers. 972 (2002)

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  • [Publications] 船津高志: "感覚器官と脳内情報処理(御子柴克彦、清水孝雄編)"共立出版. 274 (2002)

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Published: 2002-04-01   Modified: 2018-03-28  

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