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Comprehensive analyses of bacterial pathogenesis based on the genome information

Research Project

Project/Area Number 14014241
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Miyazaki (2004)
宮崎医科大学 (2002-2003)

Principal Investigator

HAYASHI Tetsuya  University of Miyazaki, Frontier Research Center, Professor, フロンティア科学実験総合センター, 教授 (10173014)

Co-Investigator(Kenkyū-buntansha) OGURA Yoshitoshi  University of Miyazaki, Frontier Research Center, Joshu, フロンティア科学実験総合センター, 助手 (40363585)
OOKA Tadasuke  University of Miyazaki, Faculty of Medicine, Joshu, 医学部, 助手 (50363594)
TOBE Toru  Osaka University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (70207596)
SHIMIZU Tohru  Kanazawa University, Graduate School of Medical Science, Professor, 大学院医学研究科, 教授 (80235655)
IIDA Tetsuya  Osaka University, Center for Infectious diseases, Associate Professor, 微生物病研究所, 助教授 (90221746)
中山 恵介  宮崎大学, 医学部, 助手 (10347057)
大西 真  宮崎医科大学, 医学部, 助教授 (10233214)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥70,500,000 (Direct Cost: ¥70,500,000)
Fiscal Year 2004: ¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 2003: ¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 2002: ¥22,500,000 (Direct Cost: ¥22,500,000)
Keywordspathogenic Escherichia coli / Clostridium perfringens / Vibrio parahaemolyticus / pathogenicity / Genomic diversity / gene regulatory network / two component regulatory system / Type III secretion system / 腸炎ビブリオ / ゲノム / マイクロアレイ / 2成分制御系 / PCR
Research Abstract

In this research, we performed a comprehensive analysis of the pathogenesis and genomic diversity of three important pathogenic bacteria based on their genome sequences, and obtained followings major findings.
1. Pathogenic Escherichia coli:
(1) Based on the O157 Sakai sequence, we developed ○a microarray and the whole genome PCR scanning method. (2) By using these two methods, we performed a large-scale genomic comparison of O157 and non-O157 enterohemorrhagic E. coli (EHEC) strains, and revealed that there exits a remarkable genomic diversity among O157 strains, and that variation of prophages and IS elements are major factors to generate this genomic diversity. Furthermore, we revealed that non-O157 EHEC strains contain a large amount of DNAs that are not present in O157. (3) We found that the RcsCDB two component regulatory system and the bicarbonate-sensing system control the virulence gene expression, and discovered novel regulators, GrvA and Pch, that are involved in these regulat … More ion systems by expression profiling anakyses using the microarray. This provided the first clue to understand how the virulence gene expression systems of pathogenic E. coli are incorporated into the intrinsic regulatory network of E. coli. (4) By employing a proteomic approach, we discovered many new effectors for the type III secretion system (TTSS) of O157.
2. Clostridium perfringen :
(1) The genome sequence of strain 13 was determined. (2) By using the microarray constructed based on its genome sequence, we identified 141 genes that are controlled by the VirR/S two component regulatory system or the VirR/S-VR-RNA system. They included not only virulence genes but also many genes for energy production and metabolism, indicating that genes for virulence and growth in host tissues are coordinately regulated by the VirR/S-VR-RNA system. (3) By searching the VirR-binding sequences, we identified two novel regulatory RNAs.
3. Vibrio parahaemolyticus :
(1) The genome sequence of strain RIMD2210633 was determined. (2) We identified two TTSSs (TTSS1 and TTSS2) on its genome, and found that TTSS1 is involved in cell toxicity and TTSS2 in diarrheagenicity. (3) We identified several effectors for each TTSS, and found that the two TTSS s exhibit distinct effector-specificities. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (116 results)

All 2006 2005 2004 2003 2002 Other

All Journal Article (85 results) Publications (31 results)

  • [Journal Article] Identification of proteins secreted via the Vibrio parahaemolyticus type III secretion system 1.2006

    • Author(s)
      T.Ono, et al.
    • Journal Title

      Infect. Immun. 74

      Pages: 1032-1042

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of proteins secreted via the Vibrio parahaemolyticus type III secretion system 1.2006

    • Author(s)
      T.Ono, et al.
    • Journal Title

      Infect.Immun. 74

      Pages: 1032-1042

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vibrios commonly possess two chromosomes.2005

    • Author(s)
      K.Okada, et al.
    • Journal Title

      J. Bacteriol. 187

      Pages: 752-757

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of a cell-wall anchored DNasegene in Clostridium perfringens.2005

    • Author(s)
      K.Okumura, et al.
    • Journal Title

      FEMS Microbiol. Lett. 242

      Pages: 281-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny.2005

    • Author(s)
      Y.Sakaguchi, et al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 102

      Pages: 17472-17477

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dual regulatory pathways integrating the RcsC-RcsD-RcsB signalling system control enterohaemorrhagic Escherichia coli pathogenicity.2005

    • Author(s)
      T.Tobe, et al.
    • Journal Title

      Mol. Microbiol. 58

      Pages: 320-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] vpaH, a gene encoding a novel H-NS-like protein that was possibly horizontally acquired, regulates the biogenesis of lateral flagella in trh-positive Vibrio parahaemolyticus2005

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Infect. Immun. 73

      Pages: 5754-5761

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microarray analysis identifies apoptosis regulatory genes expression in HCT116 cells infected with thermostable direct hemolysin gene-deletion mutants of Vibrio parahaemolyticus2005

    • Author(s)
      B.N.Bhattacharjee, et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 335

      Pages: 328-334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Vibrios commonly possess two chromosomes.2005

    • Author(s)
      K.Okada, et al.
    • Journal Title

      J.Bacteriol. 187

      Pages: 752-757

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens.2005

    • Author(s)
      K.Okumura, et al.
    • Journal Title

      FEMS Microbiol.Lett. 242

      Pages: 281-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny.2005

    • Author(s)
      Y.Sakaguchi, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 102

      Pages: 17472-17477

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dual regulatory pathways integrating the RcsC-RcsD-RcsB signalling system control enterohaemorrhagic Escherichia coli pathogenicity.2005

    • Author(s)
      T.Tobe, et al.
    • Journal Title

      Mol.Microbiol. 58

      Pages: 320-333

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] vpaH, a gene encoding a novel H-NS-like protein that was possibly horizontally acquired, regulates the biogenesis of lateral flagella in trh-positive Vibrio parahaemolyticus,2005

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Infect.Immun. 73

      Pages: 5754-5761

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microarray analysis identifies apoptosis regulatory genes expression in HCT116 cells infected with thermostable direct hemolysin gene-deletion mutants of Vibrio parahaemolyticus,2005

    • Author(s)
      B.N.Bhattacharjee, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 335

      Pages: 328-334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] III型分泌装置と細菌の病原性2005

    • Author(s)
      飯田哲也
    • Journal Title

      蛋白質・核酸・酵素 50

      Pages: 29-35

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Extensive genomic diversity in pathogenic Escherichia coli and Shigella strains revealed by comparative genomic hybridization microarray.2004

    • Author(s)
      S.Fukiya, et al.
    • Journal Title

      J. Bacteriol. 186

      Pages: 3911-3921

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytotoxicity and enterotoxicity of the thermostable direct hemolys in-deletion mutants of Vibrio parahaemolyticus.2004

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Microbiol. Immunol. 48

      Pages: 313-318

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Urease activity of enterohemorrhagic Escherichia coli depends on a specific one-base substitution in ureD.2004

    • Author(s)
      M.Nakano, et al.
    • Journal Title

      Microbiology 150

      Pages: 3483-3489

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Functional characterization of two type III secretion systems of Vibrio parahaemolyticus.2004

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Infect. Immun. 72

      Pages: 6659-6665

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biodiversity of vibrios.2004

    • Author(s)
      F.L.Thompson, et al.
    • Journal Title

      Microbiol. Mol. Biol. Rev. 68

      Pages: 403-431

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Organization and transcriptional regulation of myo-Inositol operon in Clostridium perfringens.2004

    • Author(s)
      H.I.Kawsar, et al.
    • Journal Title

      FEMS Microbiol. Lett. 235

      Pages: 289-295

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation.2004

    • Author(s)
      T.Kuwahara, et al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 101

      Pages: 14919-14924

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phages of Pseudomonas.2004

    • Author(s)
      T.Hayashi, K.Nakayama
    • Journal Title

      Pseudomonas 1

      Pages: 233-259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Extensive genomic diversity in pathogenic Escherichia coli and Shigella strains revealed by comparative genomic hybridization microarray.2004

    • Author(s)
      S.Fukiya, et al.
    • Journal Title

      J.Bacteriol. 186

      Pages: 3911-3921

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Cytotoxicity and enterotoxicity of the thermostable direct hemolysin-deletion mutants of Vibrio parahaemolyticus.2004

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Microbiol.Immunol. 48

      Pages: 313-318

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional characterization of two type III secretion systems of Vibrio parahaemolyticus.2004

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Infect.Immun. 72

      Pages: 6659-6665

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Biodiversity of vibrios.2004

    • Author(s)
      F.L.Thompson, et al.
    • Journal Title

      Microbiol.Mol.Biol.Rev. 68

      Pages: 403-431

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Organization and transcriptional regulation of myo-Inositol operon in Clostridium perfringens.2004

    • Author(s)
      H.I.Kawsar, et al.
    • Journal Title

      FEMS Microbiol.Lett. 235

      Pages: 289-295

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation.2004

    • Author(s)
      T.Kuwahara, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 14919-14924

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Phages of Pseudomonas.2004

    • Author(s)
      T.Hayashi, K.Nakayama
    • Journal Title

      Pseudomona 1

      Pages: 233-259

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytotoxicity and enterotoxicity of the thermostable direct hemoly sin-deletion mutants of Vibrio parahaemolyticus.2004

    • Author(s)
      K.-S.Park, et al.
    • Journal Title

      Microbiol.Immunol. 48

      Pages: 313-318

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 下痢・腸炎をおこす大腸菌-O157のゲノム解析からみえてきたこと2004

    • Author(s)
      林哲也
    • Journal Title

      微生物はなぜ病気をおこすか ゲノムの特徴(林英生編集)

      Pages: 58-69

    • Related Report
      2004 Annual Research Report
  • [Journal Article] クロストリジウム2004

    • Author(s)
      清水徹
    • Journal Title

      微生物はなぜ病気をおこすか ゲノムの特徴(林英生編集)

      Pages: 91-99

    • NAID

      10016519982

    • Related Report
      2004 Annual Research Report
  • [Journal Article] DNAマイクロアレイの応用2004

    • Author(s)
      清水 徹
    • Journal Title

      微生物はなぜ病気をおこすか ゲノムの特徴(林英生編集)

      Pages: 160-167

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 病原性の質と強さを見る2004

    • Author(s)
      戸邉亨
    • Journal Title

      微生物はなぜ病気をおこすか ゲノムの特徴(林英生編集)

      Pages: 170-177

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 腸管出血性大腸菌O157(enterohemorrhagic Escherichia coliO157)のゲノム解析2004

    • Author(s)
      林哲也
    • Journal Title

      ゲノミクス・プロテオミクスの新展開 生物情報の解析と応用(今中忠行監修)

      Pages: 235-247

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 腸炎ビブリオ2004

    • Author(s)
      飯田哲也
    • Journal Title

      ゲノミクス・プロテオミクスの新展開 生物情報の解析と応用(今中忠行監修)

      Pages: 248-255

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clostridium2004

    • Author(s)
      林英生, 清水徹
    • Journal Title

      ゲノミクス・プロテオミクスの新展開 生物情報の解析と応用(今中忠行監修)

      Pages: 256-267

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ゲノム解読・ゲノム比較から見えてくる細菌の病原性と多様性-病原性大腸菌O157を例として2004

    • Author(s)
      林 哲也
    • Journal Title

      わかる実験医学シリーズ ウイルス・細菌と感染症がわかる(吉開泰信編集)

      Pages: 107-113

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 病原細菌のゲノム構造2004

    • Author(s)
      林英生, 清水徹, 太田敏子
    • Journal Title

      「医学をゲノムする」阿蘇シンポジュウム記録2003

      Pages: 15-45

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ゲノムから解くヒト病原微生物 病原性ビブリオ2004

    • Author(s)
      飯田哲也
    • Journal Title

      化学と生物 42

      Pages: 619-624

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ゲノムから解くヒト病原微生物 クロストリジウム2004

    • Author(s)
      清水徹
    • Journal Title

      化学と生物 42

      Pages: 674-679

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 病原性大腸菌2004

    • Author(s)
      林哲也, 戸邉亨
    • Journal Title

      化学と生物 42

      Pages: 758-764

    • NAID

      10016520254

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ウェルシュ菌のゲノム構造の解析と病原遺伝子発現調節機構の解明2004

    • Author(s)
      清水徹
    • Journal Title

      日本細菌学雑誌 59

      Pages: 377-385

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 腸炎ビブリオの病原性とゲノム構造に関する分子遺伝学的研究2004

    • Author(s)
      飯田哲也
    • Journal Title

      日本細菌学雑誌 59

      Pages: 457-464

    • NAID

      10013501977

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ウェルシュ菌のシグナル伝達機構2004

    • Author(s)
      清水徹, 大谷郁, 中村信一
    • Journal Title

      臨床と微生物 31

      Pages: 229-237

    • NAID

      80016700496

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 病原性大腸菌のクオラムセンシングと病原性発現2004

    • Author(s)
      戸邉亨
    • Journal Title

      臨床と微生物 31

      Pages: 239-244

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 腸管出血性大腸菌のゲノム解析2004

    • Author(s)
      小椋義俊, 林哲也
    • Journal Title

      ゲノム医学 4

      Pages: 219-224

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ビブリオ属菌のゲノム解析2004

    • Author(s)
      飯田哲也, 朴権三
    • Journal Title

      ゲノム医学 4

      Pages: 337-340

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 腸管出血性大腸菌のゲノムの特徴2004

    • Author(s)
      大岡唯祐, 小椋義俊, 林哲也
    • Journal Title

      化学療法の領域 20

      Pages: 1328-1333

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V. cholerae.2003

    • Author(s)
      K.Makino, et al.
    • Journal Title

      Lancet 361

      Pages: 743-749

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli O157 : H7 isolates among Shiga toxin-producing E. coli strains.2003

    • Author(s)
      S.Makino, et al.
    • Journal Title

      J. Clin. Microbiol. 41(6)

      Pages: 2341-2347

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Shiga toxin- and enterotoxin-producing Escherichia coli isolated from subjects with bloody and non-bloody diarrhea in Bangkok, Thailand.2003

    • Author(s)
      A.Leelaporn, et al.
    • Journal Title

      Diag. Microbiol. Infect. Dis. 46

      Pages: 173-180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13.2003

    • Author(s)
      K.Ohtani, et al.
    • Journal Title

      FEMS Microbiol. Lett. 222

      Pages: 137-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The urease cluster of Vibrio parahaemolyticus does not influence expression of the thermostable direct hemolysin (TDH) gene or the TDH-related gene.2003

    • Author(s)
      Y.Nakaguchi, et al.
    • Journal Title

      Microbiol. Immunol. 47

      Pages: 233-239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Two different mechanisms are involved in the extremely high-level benzalkonium chloride resistance of a Pseudomonas fluorescens strain.2003

    • Author(s)
      K.Nagai, et al.
    • Journal Title

      Microbiol. Immunol. 47

      Pages: 709-715

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli ○157 : H7 isolates among Shiga toxin-producing E. coli strains.2003

    • Author(s)
      S.Makino, et al.
    • Journal Title

      J.Clin.Microbiol. 41

      Pages: 2341-2347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Shiga toxin-and enterotoxin-producing Escherichia coli isolated from subjects with bloody and non-bloody diarrhea in Bangkok, Thailand.2003

    • Author(s)
      A.Leelaporn, et al.
    • Journal Title

      Diag.Microbiol.Infect.Dis. 46

      Pages: 173-180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13.2003

    • Author(s)
      K.Ohtani, et al.
    • Journal Title

      FEMS Microbiol.Lett. 222

      Pages: 137-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The urease cluster of Vibrio parahaemolyticus does not influence expression of the thermostable direct hemolysin (TDH) gene or the TDH-related gene.2003

    • Author(s)
      Y.Nakaguchi, et al.
    • Journal Title

      Microbiol.Immunol. 47

      Pages: 233-239

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Two different mechanisms are involved in the extremely high-level benzalkonium chloride resistance of a Pseudomonas fluorescens strain.2003

    • Author(s)
      K.Nagai, et al.
    • Journal Title

      Microbiol.Immunol. 47

      Pages: 709-715

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genomic diversity of Escherichia coli O157 revealed by whole genome PCR Scanning.2002

    • Author(s)
      M.Ohnishi, et al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 99

      Pages: 17043-17048

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bicarbonate ion stimulates the expression of locus of enterocyte effacement-encoded genes in enterohemorrhagic Escherichia coli O157 : H7.2002

    • Author(s)
      H.Abe, et al.
    • Journal Title

      Infect. Immun. 70

      Pages: 3500-3509

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete nucleotide sequence of plasmid Rts1 ; implications for the evolution of large plasmid genomes.2002

    • Author(s)
      M.Murata, et al.
    • Journal Title

      J. Bacteriol. 184

      Pages: 3194-3202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.2002

    • Author(s)
      T.Shimizu, et al.
    • Journal Title

      J. Bacteriol. 184

      Pages: 2587-2594

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clostridial VirR/VirS regulon involves a regulatory RNA molecule for expression of toxins2002

    • Author(s)
      T.Shimizu, et al.
    • Journal Title

      Mol. Microbiol. 43

      Pages: 257-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater2002

    • Author(s)
      T.Shimizu, et al.
    • Journal Title

      Proc. Natl. Acad.Sci. USA 99

      Pages: 996-1001

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The luxS gene is involved in cell-cell signaling for toxin production in Clostridium perfringens.2002

    • Author(s)
      K.Ohtani, et al.
    • Journal Title

      Mol. Microbiol. 44

      Pages: 171-179

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a novel locus that regulates expression of toxin gene in Clostridium perfringens.2002

    • Author(s)
      K.Ohtani, et al.
    • Journal Title

      FEMS Microbiol. Lett. 209

      Pages: 109-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comparison of genome structures of vibrios, bacteria possessing two chromosomes.2002

    • Author(s)
      K.Tagomori, et al.
    • Journal Title

      J. Bacteriol. 184

      Pages: 4351-4358

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome.2002

    • Author(s)
      T.Iida, et al.
    • Journal Title

      J. Bacteriol. 184

      Pages: 4933-4935

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genomic diversity of Escherichia coli O157 revealed by whole genome PCR Scanning.2002

    • Author(s)
      M.Ohnishi, et al.
    • Journal Title

      Proc.Natl.Acad.Sci. 99

      Pages: 17043-17048

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bicarbonate ion stimulates the expression of locus of enterocyte effacement-encoded genes in enterohemorrhagic Escherichia coli O157: H7.2002

    • Author(s)
      H.Abe, et al.
    • Journal Title

      Infect.Immun. 70

      Pages: 3500-3509

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete nucleotide sequence of plasmid Rts1 ;implications for the evolution of large plasmid genomes.2002

    • Author(s)
      M.Murata, et al.
    • Journal Title

      J.Bacteriol. 184

      Pages: 3194-3202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.2002

    • Author(s)
      T.Shimizu, et al.
    • Journal Title

      J.Bacteriol. 184

      Pages: 2587-2594

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clostridial VirR/VirS regulon involves a regulatory RNA molecule for expression of toxins.2002

    • Author(s)
      T.Shimizu, et al.
    • Journal Title

      Mol.Microbiol. 43

      Pages: 257-265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater,2002

    • Author(s)
      T.Shimizu, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 99

      Pages: 996-1001

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The luxS gene is involved in cell-cell signaling for toxin production in Clostridium perfringens.2002

    • Author(s)
      K.Ohtani, et al.
    • Journal Title

      Mol.Microbiol. 44

      Pages: 171-179

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a novel locus that regulates expression of toxin gene in Clostridium perfringens,.2002

    • Author(s)
      K.Ohtani, et al.
    • Journal Title

      FEMS Microbiol.Lett. 209

      Pages: 109-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comparison of genome structures of vibrios, bacteria possessing two chromosomes.2002

    • Author(s)
      K.Tagomori, et al.
    • Journal Title

      J.Bacteriol. 184

      Pages: 4351-4358

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome.2002

    • Author(s)
      T.Iida, et al.
    • Journal Title

      J.Bacteriol. 184

      Pages: 4933-4935

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complexity of the genomic diversity of entrohaemorrhagic Escherichia coli O157 revealed by the combinational use of the O157 Sakai oligo DNA microarray and the Whole Genome PCR Scanning

    • Author(s)
      Y.Ogura, et al.
    • Journal Title

      DNA Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cortactin is essential for F-actin assembly in enteropathogenic Escherichia coli (EPEC)- and enterohaemorrhagic E.coli (EHEC)-induced pedestals and the α-helical region is involved in the localizati on of cortactin to bacterial attachment sites.

    • Author(s)
      V.V.Cantarelli, et al.
    • Journal Title

      Cell Microbiol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complexity of the genomic diversity of entrohaemorrhagic Escherichia coli O157 revealed by the combinational use of the O157 Sakai oligo DNA microarray and the Whole Genome PCR Scanning.

    • Author(s)
      Y.Ogura, et al.
    • Journal Title

      DNA Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cortactin is essential for F-actin assembly in enteropathogenic Escherichia coli (EPEC)-and enterohaemorrhagic E.coli (EHEC)-induced pedestals and the α-helical region is involved in the localization of cortactin to bacterial attachment sites.

    • Author(s)
      V.V.Cantarelli, et al.
    • Journal Title

      Cell.Microbiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] S.Makino, et al.: "Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli O157 : H7 isolates among Shiga toxin-producing E.coli strains."J.Clin.Microbiol.. 41(6). 2341-2347 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Fukiya, et al.: "Extensive genomic diversity in pathogenic Escherichia coli and Shigella strains revealed by comparative genomic hybridization microarray."J.Bact.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Leelaporn, et al.: "Shiga toxin- and enterotoxin-producing Escherichia coli isolated from subjects with bloody and non-bloody diarrhea in Bangkok, Thailand."Diag.Microbiol.Infect.Dis.. 46. 173-180 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Ohtani, et al.: "The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13."FEMS Microbiol.Lett.. 222. 137-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Shimizu: "Clostridium perfringens toxin gene regulation, transcriptome, proteome."Strict and facultative anaerobes : medical and environmental aspects.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Nakaguchi, et al.: "The urease cluster of Vibrio parahaemolyticus does not influence expression of the thermostable direct hemolysin (TDH) gene or the TDH-related gene."Microbiol.Immunol.. 47. 233-239 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.-S.Park, et al.: "Cytotoxicity and enterotoxicity of the thermostable direct hemolysin-deletion mutants of Vibrio parahaemolyticus."Microbiol.Immunol.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Nagai, et al.: "Two different mechanisms are involved in the extremely high-level benzalkonium chloride resistance of a Pseudomonas fluorescens strain."Microbiol.Immunol.. 47. 709-715 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Kuboniwa, et al.: "Quantitative detection of periodontal pathogens using real-time PCR with TaqMan probes."Oral Microbiol.Immunol.. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] 林 哲也: "ゲノム解読・ゲノム比較から見えてくる細菌の病原性と多様性-病原性大腸菌O157を例として"わかる実験医学シリーズ ウイルス・細菌と感染症がわかる(吉開泰信 編集). 107-113 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 戸邉 亨: "グラム陰性菌の病原性遺伝子探索:病原性大腸菌O157を中心に"細胞工学. 22(11). 1173-1177 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 戸邉 亨: "ゲノム情報からみた病原細菌の病原性解析〜O157を中心に"バイオベンチャー. 4(2). 30-33 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 清水 徹: "グラム陽性菌の病原遺伝子探索:ウェルシュ菌を中心に"細胞工学. 22. 1168-1172 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 飯田哲也: "細菌の病原性と遺伝子伝播"生物工学会誌. 10. 431-433 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 飯田哲也: "腸炎ビブリオのゲノム解析"日本食品微生物学会雑誌. 20. 170-175 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Ohnishi, et al.: "Genomic diversity of Escherichia coli O157 revealed by whole genome PCR Scanning"Proc.Natl.Acad.Sci.USA. 99. 17043-17048 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Abe, et al.: "Bicarbonate ion stimulates the expression of locus of enterocyte effacement-encoded genes in enterohemorrhagic Escherichia coli O157:H7"Infect.Immun.. 70. 3500-3509 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Makino, S., et al.: "Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli O157:H7 among Shiga toxin-producing E.coli"J.Clin.Microbiol.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimizu, T., et al.: "Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens"J.Bacteriol.. 184. 2587-2594 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohtani, K., et al.: "The luxS gene is involved in cell-cell signaling for toxin production in Clostridium perfringens"Mol.Microbiol.. 44. 171-179 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tagomori, K., et al.: "Comparison of genome structures of vibrios, bacteria possessing two chromosomes"J.Bacteriol.. 184. 4351-4358 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iida, T., et al.: "Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome"J.Bacteriol.. 184. 4933-4935 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Makino, K., et al.: "Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V. cholerae"Lancet. 361. 743-749 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 哲也, 大西 真: "大腸菌O157のゲノムと出血性大腸炎"現代医療. 34. 103-108 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大西 真, 林 哲也: "腸管出血性大腸菌O157のゲノムの多様性"日本臨床. 60. 1077-1082 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 哲也, 大西 真: "腸管出血性大腸菌O157の遺伝的多様性とその分子基盤"最新医学. 58. 136-141 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 哲也: "I. ゲノム微生物学概論 ゲノムからみた病原細菌"日本臨床 新世紀の感染症学(下) -ゲノム・グローバル時代の感染症アップデート-. 61. 414-422 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 哲也: "II. 感染症の遺伝子学 大腸菌"日本臨床 新世紀の感染症学(下) -ゲノム・グローバル時代の感染症アップデート-. 61. 696-702 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 戸邉 亨: "腸管出血性大腸菌の腸上皮細胞への付着と宿主感染の分子病態"日本臨床. 60. 1060-1063 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 清水 徹: "II. 感染症の遺伝子学 クロストリジウム属(ウェルシュ菌など)"日本臨床 新世紀の感染症学(下) -ゲノム・グローバル時代の感染症アップデート-. 61. 671-676 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 飯田 哲也: "II. 感染症の遺伝子学 ビブリオ属(コレラ菌,腸炎ビブリオ,ビブリオ・バルニフィカス)"日本臨床 新世紀の感染症学(下) -ゲノム・グローバル時代の感染症アップデート-. 61. 722-726 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2018-03-28  

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