シロイヌナズナにおける茎頂分裂組織構築の分子機構

• Project/Area Number: 14036222
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: 田坂 昌生 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (90179680)
• Co-Investigator(Kenkyū-buntansha): 相田 光宏 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助手 (90311787) ; 深城 英弘 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助手 (80324979)
• Project Period (FY): 2002 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥88,800,000 (Direct Cost: ¥88,800,000); Fiscal Year 2006: ¥17,700,000 (Direct Cost: ¥17,700,000); Fiscal Year 2005: ¥17,700,000 (Direct Cost: ¥17,700,000); Fiscal Year 2004: ¥17,700,000 (Direct Cost: ¥17,700,000); Fiscal Year 2003: ¥17,700,000 (Direct Cost: ¥17,700,000); Fiscal Year 2002: ¥18,000,000 (Direct Cost: ¥18,000,000)
• Keywords: シロイヌナズナ / 茎頂分裂組織 / CUC / パターン形成 / オーキシン / 胚発生 / 抵抗性反応 / 高等植物 / CUC1 / CUC2 / 転写因子 / 下流遺伝子 / マイクロアレイ

Research Abstract

1)SAM形成に関わるるCUC1(CUP-SHAPED-COTYLEDON1),CUC2遺伝子の下流候補遺伝子をマイクロアレイ解析で多数同定し、それらに対して心臓型胚における発現をin situ hybridizationで調べた。その結果、これらの多くの遺伝子が、CUCが発現する所で発現しており、cuc1cuc2二重変異株胚の対応する領域で発現が見られなかった。ただ、これらの中には、野生株でもcuclcuc2でも胚の中で他の領域で特異的な発現を示す遺伝子が幾つかあった。そして、それらの遺伝子の一つであるLAS(LATERAL SUPPRESOR)をCUC2のプロモーターで発現させた所、cuc2cuc3の地上部が示す異常を抑圧した。これは、LASがCUCの下流でSAM形成に関わる事を示唆する。
2)側根基部の細胞分裂を制御するPUCHI遺伝子の変異株は、地上部において花茎の基部に一対の突起物を生じ、その間に構造物が出来る。分子遺伝学的な解析により、この突起物は托葉である事が強く示唆された。また、花芽におけるPUCHIの発現を調べた所、花芽初期に一過的に花芽の上側に発現する事が判った。これらの結果と、野生株と変異株の初期の花芽の形態学的観察の結果から、PUCHIはシロイヌナズナの野生株において花芽分裂組織が形成される時、その根元で包葉が発達するのを阻害している可能性が示唆された。
3)シロイヌナズナの胚頂端部において、オーキシンの細胞外排出タンパク質PIN FORMED1(PIN1)の分布が2枚の線対称な子葉構築に関わり、PID(PINOID)はPIN1タンパク質の働きを後期心臓型胚期から強める事をこれまでに明らかにした。我々はpidエンハンサーmacchibou(mab)1-4を単離し解析しているが、MAB4はNPH3ファミリーのタンパク質をコードしており、PIN1タンパク質の細胞内局在に関与する事を明らかにした。そして、MAB4タンパク質が主として細胞内の機能未知の小胞に局在し、それらの小胞にPIN1やPIDも共局在する事も明らかにした。

Research Products

• [Journal Article] AUXIN RESPONSE FACTOR7 and 19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis (2007)
  • Author(s): Okushima, Y., Fukaki, H., Onoda, M., Theologis, A., Tasaka M.
  • Journal Title: Plant Cell 19 Pages: 118-130
• [Journal Article] Caged gene-inducer spatially and temporally controls gene expression and plant development in transgenic Arabidopsis plant. (2006)
  • Author(s): Hayashi, K., Hashimoto, K., Kusaka, N., Yamazoe, A., Fukaki, H., Tasaka, M., Nozaki H
  • Journal Title: Bioorg Med Chem Lett 16 Pages: 2470-2472
• [Journal Article] A C2H2-type zinc finger protein, SGR5, is involved in early events gravitropism in Arabidopsis inflorescence stems (2006)
  • Author(s): Morita T.M., Sakaguchi K., Kiyose K., Taira K., Kato T., Nakamura M., Tasaka M
  • Journal Title: The Plant Journal 47 Pages: 619-628
• [Journal Article] A role for chromatin remodeling in regulation of CUC gene expression in the Arabidopsis cotyledon boundary (2006)
  • Author(s): Kwon S, C., Hibara K., Pfluger J., Bezhani S., Metha H., Aida M., Tasaka M., Wagner D.
  • Journal Title: Development 133 Pages: 3223-3230
• [Journal Article] PICKLE is required for SOLITARY ROOT/IAA14-mediated repression of ARF7 and ARF19 activity during Arabidopsis lateral root initiation (2006)
  • Author(s): Fukaki H., Taniguchi N., Tasaka M.
  • Journal Title: The Plant Journal 48 Pages: 380-389
• [Journal Article] Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem and organ boundary formation (2006)
  • Author(s): Hibara, K., Karim, RM., Takada, S., Taoka, K., Furutani, M., Aida, M., Tasaka,M
  • Journal Title: Plant Cell 18 Pages: 2946-2957
• [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem (2005)
  • Author(s): Saito C., Morita T.M., Kato T., Tasaka M.
  • Journal Title: Plant Cell 17 Pages: 548-558
• [Journal Article] A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts (2005)
  • Author(s): Kotera E., Tasaka M., Shikanai T.
  • Journal Title: Nature 433 Pages: 326-330
• [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis (2005)
  • Author(s): Niihama M., et a.
  • Journal Title: Current Biology 15 Pages: 555-560
• [Journal Article] Physiological requirements of the nonmevaonate pathway for photo acclimation in Arabidopsis (2005)
  • Author(s): Hojo M., Tasaka M., Shikanai T.
  • Journal Title: Plant Biotechnology 22 Pages: 39-45
• [Journal Article] Tissue-speciicexpression of stabilized SORITARY-ROOT /IAA14 alters lateral root development in Arabidopsis (2005)
  • Author(s): Fukaki H., Nakao Y., Okushima Y., Theologis A., Tasaka M.
  • Journal Title: Plant J 44 Pages: 382-395
• [Journal Article] Cell cycle progression in the pericycle is not sufficient for SOLITARY-ROOT/IAA14-mediated lateral root initiation in Arabidopsis (2005)
  • Author(s): Vanneste, S., et al.
  • Journal Title: Plant Cell 17 Pages: 3035-3050
• [Journal Article] PIN-FORMED1 and PINOID regulate boundary formation and cotyledon development in Arabidopsis embryogenesis (2004)
  • Author(s): Furutani M., Vernoux T., Traas J., KatoT., Tasaka M., Aida M.
  • Journal Title: Development 31 Pages: 5021-5030
• [Journal Article] The gravitropism defective 2 (grv2) mutants of Arabidopsis are deficient in a protein implicated in a protein implicated in endocytosis (2004)
  • Author(s): McCabe R., Kato T., Lukowitz W., Sieber P., Tasaka M., Somerville C.
  • Journal Title: Plant Physiology 136 Pages: 3095-3103
• [Journal Article] The GURKE Gene Encoding an Acetyl-CoA Carboxylase is Required for Partitioning the Embryo (2004)
  • Author(s): Kajiwara T., Furutani M., Hibara K., Tasaka M.
  • Journal Title: Plant Cell Physiol 45 Pages: 1122-1128
• [Journal Article] NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON1 (CUC1)and CUP-SHAPED COTYLEDON 2 (CUC2) : domain analysis of CUC1 and CUC2. (2004)
  • Author(s): Taoka K., Yanagimoto Y., Daimon Y., Hibara K., Aida M., Tasaka M.
  • Journal Title: The Plant Journal 40 Pages: 462-473
• [Journal Article] Cyclic electron flow around photosystem I is essential for photosynthesis (2004)
  • Author(s): Munekage Y., Hashimoto M., Miyake C., Tomizawa K., Endo T., Tasaka M., Shikanai
  • Journal Title: Nature 429 Pages: 579-582
• [Journal Article] PPR motifs of the nucleus-encoded factor, PGR3, function in the selective and distinct steps of chloroplast gene expression in Arabidopsis (2004)
  • Author(s): Yamazaki H., Tasaka M, Shikanai T.
  • Journal Title: The Plant Journal 38 Pages: 152-163
• [Publications] Hibara k. et al.: "CUC1 gene activates the expression of SAM-related genes to induce adventitious shoot formation"Plant Jounal. 36. 687-696 (2003)
• [Publications] Yano D.et al.: "A SANAR complex containing SGR3/AtVAM3 and ZIG/AtVTIII in gravi-sensing cells is important for Arabidopsis gravitropism"PNAS. 100. 8589-8594 (2003)
• [Publications] Surpin M., et al.: "The VTI family of SNARE proteins is necessary for plant viability and mediates different protein transport pathways"Plant Cell. 15. 2885-2899 (2003)
• [Publications] Hasimoto M. et al.: "A nucleus-encoded factor, CRR2, is essential for the expression of chloroplast ndhB in Arabidopsis."Plant Jounal. 36. 541-549 (2003)
• [Publications] Hatakeda Y., et al.: "Gravitropic response plays an important role in the nutational movements of the shoots of Pharbitis nil and Arabidopsis thaliana"Physiologia Plantarum. 118. 464-473 (2003)
• [Publications] Daimon Y., et al.: "The CUP-SHAPED COTYLEDON genes promote adventitious shoot formation on calli"Plant Cell and Physiology. 44. 113-121 (2003)
• [Publications] Furutani, M., et al.: "Pattern Formation during Dicotyledonous Plant Embryogenesis"Morphogenesis and Pattern Formation in Biological Systems. 139-152 (2003)
• [Publications] Fukaki, H., et al.: "Lateral root formation is blocked by a gain-of-function in the SOLITARY-ROOT gene of Arabidopsis"The Plant Journal,. 29. 153-168 (2002)
• [Publications] Kato T.et al.: "SGR2, a phospholipase-like protein, and ZIG/SGR4, a SNARE, are involved in the shoot gravitropism on Arabidopsis"Plant cell. 14. 33-46 (2002)
• [Publications] Morita M.T., et al.: "Involvement of the vacuole of the endodermis in the early process of shoot gravitropism in Arabidopsis"Plant cell. 14. 47-56 (2002)
• [Publications] Aida M.et al.: "Roles of PIN-FORMED1 and MONOPTEROS in Pattern Formation of the Apical Region of the Arabidopsis Embryo"Development. 129. 3965-3974 (2002)
• [Publications] Daimon Y.et al.: "The CUP-SHAPED COTYLEDON genes promote adventitious shoot formation on calli"Plant Cell and Physiology. 44. 113-121 (2003)