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Function regulation of proteins and molecular complexes with glycosylation.

Research Project

Project/Area Number 14082101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University

Principal Investigator

FURUKAWA Koichi  Nagoya University, 大学院・医学系研究科, 教授 (80211530)

Co-Investigator(Kenkyū-buntansha) KIMATA Koji  Aichi Medical University, Institute fo medicalScience, Professor (10022641)
KINOSHITA Taro  Osaka University, Reserch Institute for Microbial Diseases, Professor (10153165)
MURAMATSU Takashi  Aichi Gakuin University, Faculty of Psychologicaland Physical science, Professor (00030891)
KNNAGI Reiji  Aichi Cancer Center, Molecular Pathology, General Manager (80161389)
KAWASAKI Toshisuke  Ritsumeikan Asia Pacific University, Center of Glyootechnology, Professor (50025706)
Project Period (FY) 2002 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥113,900,000 (Direct Cost: ¥113,900,000)
Fiscal Year 2007: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2006: ¥22,100,000 (Direct Cost: ¥22,100,000)
Fiscal Year 2005: ¥22,100,000 (Direct Cost: ¥22,100,000)
Fiscal Year 2004: ¥22,100,000 (Direct Cost: ¥22,100,000)
Fiscal Year 2003: ¥22,100,000 (Direct Cost: ¥22,100,000)
Fiscal Year 2002: ¥22,500,000 (Direct Cost: ¥22,500,000)
Keywordsglycosylation / glycosyltransferase / glycoprotein / glycolinid / proteoglycan / signal / Knolout / bioreyulation / ノックアウト / グライコミクス / 分子複合体 / ミクロドメイン / 機能調節
Research Abstract

平成14〜18年度の特定領域研究「糖鎖によるタンパク質と分子複合体の機能調節」の研究成果のとりまとめのために、主に2つの事業を実施した。
1つ目に、11月26日、27日に東京品川において公開シンポジウムを開催し、著明な成果を上げた班員を中心に研究発表を行った。実際には、日本糖鎖科学コンソーシアム第5回シンポジウムの中で本領域のセッションを設け、合計6名の講演者による発表を行い、活発な討論を行った。
6名の講演内容は岡昌吾(京都大学医学部保健学科)「神経可塑性とHNK-1糖鎖」、桝和子(筑波大学大学院)「新規のエンドスルファターゼSulfFPによるヘパラン硫酸糖鎖修飾の生理機能の解明」、北爪 しのぶ(理化学研究所)「シアル酸転移酵素の切断・分泌機構-生理的意義の解明と肝疾患マーカーとしての応用」、永井尚子(愛知医科大学 分子医化学研究所)「ヘパラン硫酸60-硫酸基転移酵素HS6ST3の細胞内局在調節機構、木下タロウ(大阪大学 微生物病研究所)「「GPIアンカー生合成酵素の欠損と疾患」、門松健治(名古屋大学大学院 生化学第一講座)神経損傷とケラタン硫酸」。
2つ目には、計画班員、公募班員の研究成果をまとめ、成果報告書を作製して班員、班友および領域の内外の研究者、省庁等に配付した。内容としては各班員による研究のまとめと共に特許申請、テレビや新聞などの取材、発表論文、主な論文のreprint等をとりまとめた。
さらに、10月17日には最終ヒヤリングを行い、全体のまとめの報告書を提出すると共に、プレゼンテーションを行い、審査委員との質疑応答を行った。A評価をいただいた。
また、審査委員会のコメントとして、本研究の内容の進展を評価すると共に、成果の応用的研究を発展させるべく、助言をいただいた。

Report

(7 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (46 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (27 results) (of which Peer Reviewed: 3 results) Presentation (3 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) Publications (12 results)

  • [Journal Article] Disruption of GM2/GD2 synthase gene resulted in overt-expression of 9-O-acetyl GD3 irrespective of Tis21.2008

    • Author(s)
      Furukawa, K., et. al.
    • Journal Title

      J. Neurochem. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of anovel phosphomannan-binding lectin from porcine serum.2007

    • Author(s)
      Ma BY, Nakamura N., et. al.
    • Journal Title

      J Biol Chem. 282(17)

      Pages: 12963-12975

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Essentioal role of heparan sulfate 2-O-sulfotransferase in chick limb bud patterning and development.2007

    • Author(s)
      Kobayashi T., et. al.
    • Journal Title

      J Biol Chem. 2007Jul 6

      Pages: 19589-97

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mice deficient in heparan sulfate 6-0-sulfotransferase-1 exhibit defective heparin sulfate biosynthesis, abnormal placentation, and late embryonic lethality.2007

    • Author(s)
      Habuchi H,., et al
    • Journal Title

      J Biol Chem. 282

      Pages: 15578-15588

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Biosynthesis of Glycolipids. In Comprehensive Glycoscience. Article2007

    • Author(s)
      Furukawa K,., et al
    • Journal Title

      Elsevier, Oxford, UK No. : 00037

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope.2006

    • Author(s)
      Shiba T,., et al
    • Journal Title

      Protein 65

      Pages: 499-508

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mice deficient in heparan sulfate 6-0-sulfotransferase-1 exhibit defective heparan sulfate biosynthesis, abnormal placentation, and late embryonic lethality.2006

    • Author(s)
      Habuchi H,., et al
    • Journal Title

      J Biol Chem. 282

      Pages: 15578-15588

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fundamental study of small interfering RNAs for ganglioside GD3 synthase gene as a therapeutic target of lung cancers2006

    • Author(s)
      Ko, K, et al.
    • Journal Title

      Oncogene 25

      Pages: 6924-6935

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Versican/PG-M regulates chondrogenesis as an extracellular matrix molecule crucial for mesenchymal condensation2006

    • Author(s)
      Kamiya N, et al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 2390-2400

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular basis of clonal expansion of hematopoiesis in 2 patients with paroxysmal nocturnal hemoglobinuria (PNH).2006

    • Author(s)
      Inoue N, et al.
    • Journal Title

      Blood 108

      Pages: 4232-4236

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Female infertility in mice deficient in midkine and pleiotrophin, which form a distinct family of growth factors2006

    • Author(s)
      Muramatsu H, et al.
    • Journal Title

      Genes Cells 11

      Pages: 1405-1417

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hypoxic culture induces expression of sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells2006

    • Author(s)
      Yin J, et al.
    • Journal Title

      Cancer Res. 66

      Pages: 2937-2945

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Physical and functional association of glucuronyltransferases and sulfotransferase involved in HNK-1 biosynthesis2006

    • Author(s)
      Kizuka Y, et al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 13644-13651

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Taegeted disruption of Gb3/CD77 synthase gene resulted in the complete deletion of globo-series glycosphingolipids and loss of sensitivity to veritoxins2006

    • Author(s)
      Okuda T, Tokuda N, Numata S, Furukawa K, et al.
    • Journal Title

      J. Biol. Chem. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular cloning and characterization of chick spacrcan2006

    • Author(s)
      Inoue Y, Yoneda M, Zhao J, Kimata K, et al.
    • Journal Title

      J. Biol. Chem. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Ganglioside GD3 promotes cell growth and invasion through pl30Cas and paxillin in malignant melanoma cells.2005

    • Author(s)
      Hamamura K,., et al
    • Journal Title

      Proc Natl Acad Sci USA 102

      Pages: 11041-11046

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ganglioside GD3 promotes cell growth and innasion through p130 Cas and paxillin in malignant melanoma cells2005

    • Author(s)
      Hmamura, K., et al
    • Journal Title

      Proc. Natl. Acad. Sci. USA 102

      Pages: 11041-11046

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ganglioside GD3 promotes cell growth and invasion through p130Cas and paxillin in malignant melanoma cells2005

    • Author(s)
      Hamamura K, Furukawa K, Hayashi T, Furukawa K, et al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA, 102

      Pages: 11041-11046

    • Related Report
      2005 Annual Research Report
  • [Journal Article] RNA polymerase II is required for siRNA generation and peri-centromeric heterochromatin formation in fission yeast2005

    • Author(s)
      Kato H, Goto DB, Martienssen RA, Urano T, Furukawa K, et al.
    • Journal Title

      Science 309

      Pages: 467-469

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A non-sulfated form of the HNK-1 carbohydrate is expressed in mouse kidney2005

    • Author(s)
      Tagawa H, Kizuka Y, Ikeda T, Itoh S, Kawasaki T, et al.
    • Journal Title

      J. Biol. Chem., 280

      Pages: 23876-23883

    • Related Report
      2005 Annual Research Report
  • [Journal Article] N-Acetylglucosamine-6-O-sulfotransferases 1 and 2 cooperatively control lymphocyte homing through L-selectin ligand biosynthesis in high endothelial venules2005

    • Author(s)
      Kawashima H, Petryniak B, K, Kadomatsu K, Muramatsu T, et al.
    • Journal Title

      Nat. Immunol. 6

      Pages: 1096-1104

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Over-expressed GM1 suppresses NGF signals by modulating the intracellular localization of NGF receptors and membrane fluidity in PC12 cells.2004

    • Author(s)
      Furukawa, K. et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 33368-33378

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Carbohydrate Mimicry between Human Ganglioside GM1 and Campylobacter jejuni Lipo-oligosaccharide Causes Guillain-Barre Syndrome.2004

    • Author(s)
      Furukawa, K. et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 11404-11409

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Glycosphingolipids in engineered mice.2004

    • Author(s)
      Furukawa, K. et al.
    • Journal Title

      Semin.Cell Dev.Biol. 15

      Pages: 389-396

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 糖鎖科学の新局面 実験医学 特集 糖鎖シグナルによる多彩な生体機能制御 シグナル伝達、細胞分裂、神経ネットワーク形成制御から疾患まで2004

    • Author(s)
      古川 鋼一
    • Journal Title

      実験医学 22

      Pages: 912-915

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli.2004

    • Author(s)
      Kawasaki, T. et al.
    • Journal Title

      Protein Expr.Purif. 35

      Pages: 111-119

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation.2004

    • Author(s)
      Kimata, K. et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 18679-18687

    • NAID

      110003994219

    • Related Report
      2004 Annual Research Report
  • [Presentation] Molecular complex formation based on glycoshingolipids as a functional plet from to support cancer cells2007

    • Author(s)
      Koichi Furukawa
    • Organizer
      第80回日本生化学学会大会、第30回日本分子生物学会年会の合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] Molecular complex formation based on glycosphingolipids as a functional platform to support cancer cells2007

    • Author(s)
      Koichi Furukawa
    • Organizer
      第80回日本生化学学会大会、第30回日本分子生物学会年会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Molecular complex formation based on Glycoshiyslipids as a functional platform to support cancer cells.2007

    • Author(s)
      Koichi Furukawa., et al
    • Organizer
      Compined Annnal Meeting of Japanse Societies of Biochemistry and Molecular Biology.
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Knockout mice and glycolipids. In Comprehensive Glycoscience. Article No, :00086 pp149-1572007

    • Author(s)
      Furukawa, K., et. al.
    • Total Pages
      800
    • Publisher
      Elsevier Ltd
    • Related Report
      2007 Annual Research Report
  • [Book] Biosynthesis of Glycolipids. In Comprehensive Glycoscience. Article No. : 00037, pp1O5-1142007

    • Author(s)
      Furukawa K,., et al
    • Total Pages
      800
    • Publisher
      Elsevier, Oxford, UK
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.glycomics.ne.jp/

    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ヘパラン硫酸6-O-硫酸基転移酵素2006

    • Inventor(s)
      羽渕 弘子, 他
    • Industrial Property Rights Holder
      生化学工業株式会社
    • Acquisition Date
      2006-06-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Publications] Chen, H.H.et al.: "Suppression of lung metastasis of mouse Lewis lung cancer P29 with transfection of the ganglioside GM2/GD2 synthase gene."Int.J.Cancer. 103. 169-176 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Furukawa, K., Horie, M., Okutomi, K., Sugano, S., Furukawa.K.: "Isolation and functional analysis of the melanoma specific promoter region of human GD3 synthase gene."Biochim.Biophys.Acta.. 1627. 71-78 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsuchida, A.et al.: "Synthesis of disialyl Lewis a structure in colon cancer cell lines by a sialyltransferase ST6GalNAc VI responsible for the syntheis of α -series gangliosides."J.Biol.Chem.. 278. 22787-22794 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwamura, K. et al.: "The blood group P1 synthase gene is identical to the Gb3/CD77 synthase gene : A solution of the P1/P2/p puzzle."J.Biol.Chem.. 278. 44429-44438 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yada, T.et al.: "Chondroitin sulfate synthase-3. Molecular cloning and characterization."J.Biol.Chem.. 278. 39711-39725 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kakuda, S.et al.: "Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1."J.Biol.Chem.. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mitsuda, T. et al.: "Over-expression of ganglioside GM1 results in the dispersion of platelet derived growth factor receptor from glycolipid-enriched microdomains and in the suppression of cell growth signals"J.Biol.Chem.. 277. 11239-11246 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue, M. et al.: "Refractory skin injury in the complex knock-out mice expressing only GM3 ganglioside"J.Biol.Chem.. 277. 29881-29888 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura, Y. et al.: "Identification of a Drosophila gene encoding xylosylprotein b4-galactosyltransferase that is essential for the synthesis of glycosaminoglycans and for morphogenesis"J.Biol.Chem.. 277. 46280-46288 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Itano, N. et al.: "Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration"Proc.Natl.Acad.Sci. USA. 99. 3609-3614 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shibata, Y. et al.: "Nuclear targeting by the growth factor midkine"Mol.Cell.Biol.. 22. 6788-6796 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohishi, K., Nagamune, K., Maeda, Y., Kinoshita, T.: "Two subunits of GPI transamidase GPI8 and PIG-T form a functionally important intermolecular disulfide bridge"J.Biol.Chem.. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2018-03-28  

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