種形成の分子機構の総括

• Project/Area Number: 14087101
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 岡田 典弘 Tokyo Institute of Technology, 大学院・生命理工学研究科, 教授 (60132982)
• Co-Investigator(Kenkyū-buntansha): 堀 道雄 京都大学, 大学院・理学研究科, 教授 (40112552) ; 澤村 京一 筑波大学, 大学院・生命環境科学研究科, 講師 (90247205) ; 石田 直理雄 産業技術総合研究所, 生物機能工学研究部門, 生物時計グルーブ長 (00344234) ; 長谷部 光泰 大学共同利用機関法人, 基礎生物学研究所, 教授 (40237996) ; 荻原 保成 横浜市立大学, 木原生物学研究所, 教授 (40185533) ; 小熊 譲 筑波大学, 生物科学系, 教授 (90114074) ; 舘田 英典 九州大学, 大学院・理学研究院, 教授 (70216985) ; 渡邉 正勝 東京工業大学, 助手 (90323807)
• Project Period (FY): 2002 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥52,800,000 (Direct Cost: ¥52,800,000); Fiscal Year 2008: ¥1,500,000 (Direct Cost: ¥1,500,000); Fiscal Year 2007: ¥6,300,000 (Direct Cost: ¥6,300,000); Fiscal Year 2006: ¥11,400,000 (Direct Cost: ¥11,400,000); Fiscal Year 2005: ¥4,800,000 (Direct Cost: ¥4,800,000); Fiscal Year 2004: ¥8,800,000 (Direct Cost: ¥8,800,000); Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 2002: ¥17,600,000 (Direct Cost: ¥17,600,000)
• Keywords: シクリッド / ビクトリア湖 / 種形成 / ショウジョウバエ / シロイヌナズナ / コムギ / 集団遺伝 / 多様化 / パンコムギ

Research Abstract

特定領域研究「種形成の分子機構」は「生物の多様性が如何にして獲得されたのか?」という生物学における最も大きな問題を分子レベルで解き明かしていく事を目標としてきた。この問題は進化論が発表されて以来、多くの研究者が興味を持ち続けてきたテーマでもあるが、実際の研究方法を見出すことにおいて困難を極めていた。そこで我々は植物から昆虫、脊椎動物など様々な生物種を用い、統合的な視点から種の分化という問題に取り組んできた。この研究領域は平成19年度には終了していたが、その研究成果の取りまとめのために、本年度も期間を延長して研究を実施した次第である。本年度において、我々はこれまでに研究を続けてきたヴィクトリア湖シクリッド研究に関して次その生態調査で集めた膨大なシクリッドの生体情報を体系的にまとめあげ、「ヴィクトリア湖南部のシクリッド-種形成の現場]としてカラー図版を出版した。この図版は生きた状態のシクリッドカラー写真が1000枚以上掲載され、総ページ数が250を超えており次可能な限りの情報を組み込んである。この図版はシクリッド研究者だけでなく多くの進化生物学者にとって重要な意味合いをもつだろう。また、我々の視覚研究の内容が2008年のNature誌に掲載され、さらには、News and Viewsにも紹介されるなど、本特定領域研究で実施してきたこれまでの研究を世界に向けて発信することができたのではないかと考えている。

Research Products

• [Journal Article] Speciation of Drosophila : A Role for Clock Genes in the Timing of Species-specific Mating and Locomotor Behavior (2006)
  • Author(s): N.Ishida, I.Nishinokubi, M.Shimoda
  • Journal Title: Formosan Entomol. 26 Pages: 227-234
• [Journal Article] Mating rhythms of Drosophila : rescue of tim01 mutants by D.ananassae timeless (2006)
  • Author(s): I.Nishinokubi, M.Shimoda, N.Ishida
  • Journal Title: Journal of Circadian Rhythms 4:4 Pages: 1-6
• [Journal Article] Transposon-mediated gene trapping in zebrafish. (2006)
  • Author(s): Kotani, T., Nagayoshi, S., Urasaki, A., Kawakami, K.
  • Journal Title: Methods 39 Pages: 447-462
• [Journal Article] Establishing interspecific mosaic genome lines between Drosophila ananassae and Drosophila pallidosa by means of parthenogenesis. (2006)
  • Author(s): K.Sawamura, Y.Tomimura, H.Sato, H.Yamada, M.Matsuda, Y.Oguma
  • Journal Title: Genetical Researc 88 Pages: 1-11
• [Journal Article] Parallelism of amino acid changes at the RH1 affecting spectral sensitivity among deep-water cichlids from Lakes Tanganyika and Malawi. (2005)
  • Author(s): Sugawara, T., Terai, Y., Imai, H., Turner, G.F., Koblmuller, S., S
  • Journal Title: Proceedings of the National Academy of Sciences USA 102 Pages: 5448-5453
• [Journal Article] A population genetic model for lateral dimorphism frequency in fishes. (2005)
  • Author(s): Nakajima, M., Matsuda, H., Hori, M.
  • Journal Title: Population Ecology 47 Pages: 83-90
  • NAID: 40006818465
• [Journal Article] Simple synthesis of the major sex pheromone components of Drosophila ananassae and D. pallidosa. (2005)
  • Author(s): Morita, A., Matsuyama, S., Oguma, Y., Kuwahara, S.
  • Journal Title: Bioscience Biotechnology and Biochemistry 69 Pages: 1620-1623
  • NAID: 130000030255
• [Journal Article] Genome-wide Expression Analysis Reveals 100 Adrenal Gland dependent Circadian Genes in the Mouse Liver (2005)
  • Author(s): Oishi K., Amagai N., Shirai H., Kadota K., Ohkura N., Ishida N.
  • Journal Title: DNA Research 12 Pages: 191-202
• [Journal Article] A Role for Glycogen Synthase Kinase-3β in the Mammalian Circadian Clock (2005)
  • Author(s): Iitaka C., Miyazaki K., Akaike T., Ishida N.
  • Journal Title: The Journal of Biological Chemistry 280 Pages: 29397-29402
• [Journal Article] The modified ABC model explains the development of the petaloid perianth of Agapanthus praecox ssp. orientalis (Agapanthaceae) flowers. (2005)
  • Author(s): Nakamura, T., Fukuda, T., Nakano, M., Hasebe, M., Kameya, T., Kanno, A.
  • Journal Title: Plant Molecular Biology 58 Pages: 435-445
• [Journal Article] Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome. (2005)
  • Author(s): Ogihara, Y., Yamazaki, Y., Murai, K., Kanno, A., Terachi, T., Shiina, T., Miyashita, N., Nasuda, S., Nakamura, C., Mori, N., Takumi, S.
  • Journal Title: Nucleic Acid Research 33 Pages: 6235-6250
• [Journal Article] The evolution of genes for pigmentation in African cichlid fishes (2004)
  • Author(s): Ayano Sugie, Yohey Terai, Ryoko, Ota, Norihiro Okada
  • Journal Title: GENE 343/2 Pages: 337-346
• [Journal Article] Persistence and fluctuation of lateral dimorphism in fishes. (2004)
  • Author(s): Nakajima, M., Matsuda, H., Hori, M
  • Journal Title: American Naturalis 163(5) Pages: 692-698
• [Journal Article] Female mate recognition and sexual isolation depending on courtship song in Drosophila sechellia and its siblings (2004)
  • Author(s): Masatoshi Tomaru, Hirokazu Yamada, Yuzuru Oguma
  • Journal Title: Genes and Genetic Systems 79(3)
  • NAID: 130000062110
• [Journal Article] Feeding is not a more potent Zeitgeber than the light-dark cycle in Drosophila (2004)
  • Author(s): Oishi K., Shiota M., Sakamoto K., Kasamatsu M., Ishida N
  • Journal Title: NeuroReport 15(4)
• [Journal Article] Characterization of MADS-box genes in charophycean green alga and its implication for the evolution of MADS-box genes. (2004)
  • Author(s): Tanabe^*, Y., Hasebe^*, M., Sekimoto, H., Nishiyama, T., Kitani, M., Henschel, K., Munster, T., Theissen, G., Nozaki, H., Ito, M.(^*both authors contributed equally)
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 2436-2441
• [Journal Article] Characterization and functional analysis of three wheat genes with homology to the CONSTANS flowering time gene in transgenic rice. (2004)
  • Author(s): Nemoto, Y., M.Kisaka, T.Fuse, M.Yano, Y.Ogihara
  • Journal Title: Plant J 36 Pages: 82-93
• [Book] ヴィクトリア湖南部のシクリッド-種形成の現場 (2008)
  • Author(s): 溝入真治、相原光人、岡田典弘(主執筆者)
  • Total Pages: 273
  • Publisher: 工学図書株式会社
• [Remarks]
  • URL: http://www.evolution.bio.titech.ac.jp/
• [Remarks]
  • URL: http://kawakami.lab.nig.ac.jp/
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  • URL: http://ecol.zool.kyoto-u.ac.jp/
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  • URL: http://unit.aist.go.jp/brf/ci/research/group/ishida/index.html
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  • URL: http://www.nibb.ac.jp/evodevo
• [Remarks]
  • URL: http://pgenome.sci.yokohama-cu.ac.jp/
• [Remarks]
  • URL: http://www.evolution.bio.titech.ac.jp/
• [Remarks]
  • URL: http://unit.aist.go.jp/brf/ci/research/group/ishida/index.html
• [Remarks]
  • URL: http://pgenome.sci.yokohama-cu.ac.jp/
• [Remarks]
  • URL: http://kawakami.lab.nig.ac.jp
• [Remarks]
  • URL: http://www.nibb.ac.jp/evodevo
• [Remarks]
  • URL: http://ecol.zool.kyoto-u.ac.jp/
• [Publications] Terai Y., Morikawa N., Kawakami K., Okada N.: "The complexity of alternative splicing of hagoromo mRNA is increased in an explosively speciated lineage in East African cichlids"Proc Natl Acad Sci U S A.. 100. 12798-803 (2003)
• [Publications] Kassam, D.: "Morphometric analysis on ecomorphologically equivalent cichlid species from Lake Tanganyika"Zoologica Science. 1-5 (2003)
• [Publications] T Miyo, Y Oguma, B Charlesworth: "The comparison of intrinsic rates of increase among chromosome-substituted lines resistant and susceptible to organophosphate insecticides in Drosophila melanogaster"Jpn.J.Genet.. 78(5). 373-382 (2003)
• [Publications] Katsutaka Oishi, Koyomi Miyazaki, Koji Kadota, Reiko Kikuno, Takahiro Nagase, Gen-ichi Atsumi, Naoki Ohkura, Takashi Azama, Miho Mesaki, Shima Yukimasa, Hisato Kobayashi, Chisato Iitaka, Takashi Umehara, Masami Horikoshi, Takashi Kudo, Yoshihisa Shimizu, Masahiko Yano, Morito Monden, Kazuhiko Machida, Juzo Matsuda, Shuichi Horie, Takeshi Todo, Norio Ishida: "Genome-wide Expression Analysis of Mouse Liver Reveals CLOCK-regulated Circadian Output Genes"Journal of Biological Chemistry. 278. 41519-41527 (2003)
• [Publications] Nishiyama, T., Fujita, T., Shin-I, T., Seki, M., Nishide, H., Uchiyama, I., Kamiya, A., Carninci, P., Hayashizaki, Y., Shinozaki, K., Kohara, Y., Hasebe, M.: "Comparative genomics of Physcomitrella patens gemetophytic transcriptome and Arabidopsis thaliana : Implication for land plant evolution."Proc.Natl.Acad.Sci.USA. 100. 8007-8012 (2003)
• [Publications] Ogihara, Y., K.Mochida, Y.Nemoto, K.Murai, Y.Yamazaki, T.Shin-I, Y.Kohara: "Correlated clustering and virtual display of gene expression patterns in the wheat life cycle by large-scale statistical analyses of expressed sequence tags."Plant Journal. 33. 1001-1011 (2003)
• [Publications] Terai, Y., Mayer, W., E.Klein, J.Tichy, H., Okada, N.: "The effect of selection on a long wavelength-sensitive (LWS) opsin gene of Lake Victoria cichlid fishes"Proc Natl Acad Sci USA. 99. 15501-15506 (2002)
• [Publications] Takahashi, T., Hori, M., Nakaya, K.: "Cyprichromis sonatus, a new species of the Tanganyikan cichlid fish"Copeia. 4. 1029-1036 (2002)
• [Publications] H.Yamada, M.Matsuda, Y.Oguma: "Genetics of sexual isolation based on courtship song between two sympatric species : Drosophila ananassae and D.pallidosa"Genetica. 116. 225-237 (2002)
• [Publications] Inoue S, Shimoda M, Nishinokubi I, Siomi M, Okamura M, Nakamura A, Kobayashi S, Ishida N, Siomi H: "A role for the Drosophila fragile x-related gene in circadian output"Curr.Biol.. 12. 1331 (2002)
• [Publications] Ishikawa, H., Ueno, Y., Semiarti, E., Onouchi, H., Kojima, S., Tsukaya, H., Hasebe, M., Soma, T., Ikezaki, M., Machida, C., Machida, Y.: "The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper"Plant Cell Phys. 43. 467-478 (2002)
• [Publications] Matsuoka, Y., Y.Yamazaki, Y.Ogihara, K.Tsunewaki: "Whole chloroplast genome comparison of rice, maize, and wheat : Implications for chloroplast gene diversification and phylogeny of cereals"Molecular Biology and Evolution. 19. 2084-2091 (2002)