シクリッドの爆発的多様化の分子機構

• Project/Area Number: 14087202
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 岡田 典弘 Tokyo Institute of Technology, 大学院・生命理工学研究科, 教授 (60132982)
• Co-Investigator(Kenkyū-buntansha): 二階堂 雅人 東京工業大学, 大学院・生命理工学研究科, 助教 (70432010) ; 高橋 一彦 大学共同利用機関法人, 自然科学研究機構基礎生物学研究所, 助教 (40302955) ; 川上 浩一 国立遺伝学研究所, 個体遺伝研究系, 准教授 (70195048) ; 渡邉 正勝 (渡邊 正勝) 東京工業大学, 大学院生命理工学研究科, 講師 (90323807) ; 佐藤 哲 WWFジャパン, 世界自然保護基金, 自然保護室長・研究職 ; 大島 一彦 東京工業大学, 大学院・生命理工学研究科, 講師 (60282852)
• Project Period (FY): 2002 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥256,600,000 (Direct Cost: ¥256,600,000); Fiscal Year 2007: ¥47,500,000 (Direct Cost: ¥47,500,000); Fiscal Year 2006: ¥47,500,000 (Direct Cost: ¥47,500,000); Fiscal Year 2005: ¥44,800,000 (Direct Cost: ¥44,800,000); Fiscal Year 2004: ¥44,800,000 (Direct Cost: ¥44,800,000); Fiscal Year 2003: ¥36,000,000 (Direct Cost: ¥36,000,000); Fiscal Year 2002: ¥36,000,000 (Direct Cost: ¥36,000,000)
• Keywords: シクリッド / 種分化 / 種形成 / 多様化 / 視覚 / 嗅覚 / 額部 / 光受容体遺伝子 / 顎・歯 / 性決定 / 比較ゲノム / 進化

Research Abstract

1)本年度までに視覚の適応を介した種の分化と形成の分子機構が明らかにし、この機構がビクトリア湖産シクリッドの共通の機構である可能性を示唆した。また水深や透明度などビクトリア湖の様々な環境に生息する種の視覚関連遺伝子が、生息環境の光に適応するように分化していることが示された。そこで今年度はこれらの遺伝子の配列から光受容体を再構築し吸収波長の測定を行った。その結果、これらの遺伝子が機能的にもそれぞれの生息環境の光に適応してきたことが明らかになった。また視覚関連遺伝子の近傍領域の配列を用いて系統樹を構築し、ビクトリア湖の種の適応の歴史を推定した。その結果、ビクトリア湖の種は透明度の低い河川の環境から深場や透明度の高い岩場などの湖特異的環境に適応して来たことが推定された。
2)哺乳類フェロモン受容体ホモログであるVIR遺伝子をシクリッドから単離したところ、、これまで単一の遺伝子だと考えられていたこの遺伝子が6つの遺伝子からなる遺伝子ファミリーを形成していることが明らかになった。このうち2っの遺伝子はビクトリア湖の種間でそのアミノ酸配列が多様化しており、また種特異性も見られた。このようなアミノ酸の多様化と配列の種特異性は、これまで種の分化形成に関与してきたことを明らかにした。視覚関連遺伝子のみで見られていた。そのため、このVIR遺伝子も嗅覚を介して種の分化形成に関与してきた可能性が高いと考えられる。
3)モデル脊椎動物ゼブラフィッシュにおいて、エンハンサートラップ法を開発した。これにより発生関連遺伝子の変異の分離と機能解析が可能となった。またトランスポゾンを用いたGa14-UAS法を確立した。これにより特定の細胞で任意の遺伝子を発現させ機能解析することが可能になった.

Research Products

• [Journal Article] Heterotypy in the N-terminal region of growth/Differentiatiation Factor 5 (GOF5) mature protein during teleost evolution (2008)
  • Author(s): Fujimura K, Terai Y. Ishiguro N, Miya M, Nishida M, Okada N
  • Journal Title: Mol Biol Evol. (in press)
  • Peer Reviewed
• [Journal Article] Rooting the eutherian tree: the power and pitfalls of phylogenomica. (2008)
  • Author(s): Nishihara H, Okada N, Hasegawa M.
  • Journal Title: Genome Biol. 8 Pages: 199-199
  • Peer Reviewed
• [Journal Article] Possible Involvement of SINEs in mammalian-specific brain formation. (2008)
  • Author(s): Sasaki T, Nishihara H, Hirakawa M, fujimura K, Tanaka M, Kokubo N, Kimura-Yoshida C, Matsuo I, Sumiyama K, Saitou N, Shimogori T, Okada N.
  • Journal Title: Proc Natl Acad Sci USA. 105 Pages: 4220-4225
  • Peer Reviewed
• [Journal Article] Genetic dissection of neural circuits by To12 transposon-mediated Ga14 gene and enhancer trapping in zebrafish (2008)
  • Author(s): Asakawa, K., Suster, M. L., , Mizusawa, K., Nagayoshi, S., Kotani, T., Urasaki, A., Kishimoto, Y., Hibi, M., Kawakami, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 1255-1260
  • Peer Reviewed
• [Journal Article] Insertional Mutagenesis by the To12 transposon-mediated enhancer trapapproach generated mutations in two developmental genes: tcf7 and synembryn-like (2008)
  • Author(s): Nagayoshi, S., Hayashi, E., Abe, G., Osato, N., Asakawa, K., Urasaki, A., Horikawa, K., Ikeo, K., Takeda, H., Kawakami, K.
  • Journal Title: Development 135 Pages: 159-169
  • Peer Reviewed
• [Journal Article] Development of the embryo, larva and early juvenile of Nile tilapia Oreochromis niloticus (Pisces: Cichlidae) Developmental staging system (2007)
  • Author(s): Fujimura K, Okada N.
  • Journal Title: Develop. Growth Differ. 49 Pages: 301-324
  • NAID: 10019935075
  • Peer Reviewed
• [Journal Article] MyrSINEs: a novel SINE family in the anteater genomes. (2007)
  • Author(s): Nishihara H, Kuno S, Nikaido M, Okada N
  • Journal Title: Gene 400 Pages: 98-103
  • Peer Reviewed
• [Journal Article] Acquisition of Endonuclease Specificity During Evolution of Ll Retrotransposon. (2007)
  • Author(s): Ichiyanagi K, Nishihara H, Duvernell DD, Okada N.
  • Journal Title: Mol Biol Evol. 9 Pages: 2009-2015
  • Peer Reviewed
• [Journal Article] Poxviruses as possible vectors for horizontal transfer of retroposons from reptiles to mammals. (2007)
  • Author(s): Piskurek O, Okada N.
  • Journal Title: Proc Natl Acad Sci U S A. 104 Pages: 12046-12051
  • Peer Reviewed
• [Journal Article] Novel SINE Families from Salmons Validate Parahucho (Salmonidae) as a Distinct Genus and Evidence that SINEs Can Incorporate LINE-related 3'-Tails of Other SINEs. (2007)
  • Author(s): Matveev V, Nishihara H, Okada N.
  • Journal Title: Mol Biol Evol. 24 Pages: 1656-1666
  • Peer Reviewed
• [Journal Article] Genomic expansion of the Bov-A2 retroposon relating to phylogeny and breed management. Mamm Genome. (2007)
  • Author(s): Onami J, Nikaido M, Mannen H, Okada N.
  • Journal Title: Mamm Genome. 3 Pages: 187-196
  • Peer Reviewed
• [Journal Article] Toothed whale monophyly reassessed by SINE insertion analysis: The absence of lineage sorting effects suggests a small population of a common ancestral species. (2007)
  • Author(s): Nikaido M, Piskurek O, Okada N.
  • Journal Title: Mol. Phylogenet. Evol. 43 Pages: 216-22
  • Peer Reviewed
• [Journal Article] Mitogenomic analysis for coelacanths (Latimeria chalumnae) caught in Tanzania. (2007)
  • Author(s): Sasaki T, Sato T, Miura S, Bwathondi PO, Ngatunga BP, OKada N.
  • Journal Title: Gene. 389 Pages: 73-79
  • Peer Reviewed
• [Journal Article] Novel retrotransposon analysis reveals multiple mobility pathways dictated by hosts. (2007)
  • Author(s): Ichiyanagi K, Nakajima R, Kajikawa M, Okada N.
  • Journal Title: Genome Res. 1 Pages: 33-41
  • Peer Reviewed
• [Journal Article] Newly discovered young CORE-SINEs in marsupial genomes. (2007)
  • Author(s): Munemasa M, Nikaido M, Nishihara H, Donnellan S, Austion CC, Okada N.
  • Journal Title: Gene 407 Pages: 176-185
  • Peer Reviewed
• [Journal Article] To12: a versatile gene transfer vector in vertebrates (2007)
  • Author(s): Kawakami, K.
  • Journal Title: Genome Biology 8 Pages: 7-7
  • Peer Reviewed
• [Journal Article] Nodal signals mediate interactions between the extra-embryonic and embryonic tissues in zebrafish. (2007)
  • Author(s): Fan, X., Hagos, E. G., Xu, B., Sias, C., Kawakami, K., Burdine, R. D., and Dougan, S. T.
  • Journal Title: Developmental biology 310 Pages: 363-378
  • Peer Reviewed
• [Journal Article] A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. (2007)
  • Author(s): Seguchi, O., takashima, S., Yamazaki, S., Asakura, M., Asano, Y., Shintani, Y., Wakeno, M., Minamino, T., Kondo, H., Furukawa, H., Nakamaru, K., Naito, A., takahashi, T., Ohtsuka, T., Kawakami, K., Isomura, T., Kitamura, S., Tomoike, H., Mochizuki, N., an
  • Journal Title: The Journal of Clinical Investigation 117 Pages: 2812-2824
  • Peer Reviewed
• [Journal Article] Localization of ammonia transporter Rhcgl in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression. (2007)
  • Author(s): Nakada, T., Hoshijima, K., Esaki, M., Nagayoshi, S., Kawakami, K., Hirose, S.
  • Journal Title: Am J Physiol regul Integr Comp Physiol 293 Pages: 1743-1753
  • Peer Reviewed
• [Journal Article] Targeting neural circuitry in zebrafish using GAL4 enhancer trapping (2007)
  • Author(s): Scott, E. K., Mason, L., Arrenberg, A. B., Ziv, L., Gosse, N. J., Xiao, T., Chi, N. C., Asakawa, K., Kawakami, K., Baier, H
  • Journal Title: Nature Methods 48 Pages: 323-326
  • Peer Reviewed
• [Journal Article] 脊椎動物におけるトランスポゾンを用いた遺伝学的方法論 (2007)
  • Author(s): 浦崎明宏, 川上浩一
  • Journal Title: 実験医学 25 Pages: 2507-2512
• [Journal Article] To12トランスポゾンを用いたゼブラフィッシュGaL4エンハンサートラップ法の確立 (2007)
  • Author(s): 浅川和秀, 川上浩一
  • Journal Title: バイオテクノロジージャーナル 7 Pages: 603-606
• [Journal Article] Divergent Selection on Opsins Drives Incipient Speciation in Lake Victoria Cichlids. (2006)
  • Author(s): Terai Y, Seehausen O, Sasaki T, Takahashi K, Mizoiri S, Sugawara T, Sato T, Watanabe M, Konijnendijk N, Mrosso HD, Tachida H, Imai H, Shichida Y, Okada N
  • Journal Title: PLoS Biol. 5;4(12)
  • NAID: 120006622436
• [Journal Article] Pigment pattern in jaguar/obelix Zebrafish Is Caused by a Kir7.1 Mutation : Implications for the Regulation of Melanosome Movement. (2006)
  • Author(s): Iwashita M, Watanabe M, Ishii M, Chen T, Johnson SL, Kurachi Y, Okada N, Kondo S
  • Journal Title: PLoS Genet. 24;2(11)
• [Journal Article] Spot pattern of leopard Danio is caused by mutation in the zebrafish connexin41.8 gene. (2006)
  • Author(s): Watanabe M, Iwashita M, Ishii M, Kurachi Y, Kawakami A, Kondo S, Okada N
  • Journal Title: EMBO Rep. 7(9) Pages: 893-897
• [Journal Article] magp4 gene may contribute to the diversification of cichlid morphs and their speciation (2006)
  • Author(s): Kobayashi N, Watanabe M, Kijimoto T, Fujimura K, Nakazawa M, Ikeo K, Kohara Y, Gojobori T, Okada N
  • Journal Title: Gene 373 Pages: 126-133
• [Journal Article] Transposon-mediated gene trapping in zebrafish. (2006)
  • Author(s): Kotani, T., Nagayoshi, S., Urasaki, A., Kawakami, K
  • Journal Title: Methods 39 Pages: 199-206
• [Journal Article] Functional dissection of the Tb12 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition. (2006)
  • Author(s): Akihiro Urasaki, Ghislaine Morvan, Koichi Kawakami
  • Journal Title: Genetics 174 Pages: 639-649
• [Journal Article] Parallelism of amino acid changes at the RH1 affecting spectral sensitivity among deep-water cichlids from Lakes Tanganyika and Malawi. (2005)
  • Author(s): Sugawara, T., Terai, Y., Imai, H., Turner, G.F., Koblmuller, S., S
  • Journal Title: Proceedings of the National Academy of Sciences USA 102 Pages: 5448-5453
• [Journal Article] cimp1, A Novel Astacin Family Metalloproteinase Gene from East African Cichlids, Is Differentially Expressed Between Species During Gr (2005)
  • Author(s): Kijimoto, T., Watanabe, M., Fujimura, K., Nakazawa, M., Murakami
  • Journal Title: Molecular Biology and Evolution 22 Pages: 1649-1660
• [Presentation] The diversity of opsins in sympatric Laka Victoria cichlid species (2007)
  • Author(s): Ryutaro Miygai, Yohey Terai, Mitsuto Aibara, Shinji Mizoiri and Norihiro Okada
  • Organizer: Asia-Africa evolution meeting
  • Place of Presentation: 千葉・生命の森リゾート
  • Year and Date: 2007-12-05
• [Presentation] A new approach to resolve the relationship among the closely related cichlid species in Lake Victoria (2007)
  • Author(s): Naoki Kobayashi, Masakatsu Watanabe, Jun-ichi Asakawa, and Norihiro Okada
  • Organizer: Asia Africa Evolution meeting
  • Place of Presentation: 千葉・生命の森リゾート
  • Year and Date: 2007-12-05
• [Presentation] Adaptation of opsins causes the species diversity of Lake Victoria cichlid fishes (2007)
  • Author(s): Yohey Terai, Ryutaro Miygai, Shinji Mizoiri, Mistuto Aibara, Hiroo Imai, Semvua I. Mzighani, Hidenori Tachida^3, and Norihiro Okada^1
  • Organizer: Asia-Africa evolution meeting
  • Place of Presentation: 千葉・生命の森リゾート
  • Year and Date: 2007-12-05
• [Presentation] 同所的に生息するシクリッド種間でのオプシンの多様性 (2007)
  • Author(s): 宮城竜太郎, 寺井洋平, 相原光人, 溝入真治, 岡田典弘
  • Organizer: 日本進化学会 第9回大会
  • Place of Presentation: 京都・京都大学
  • Year and Date: 2007-09-01
• [Presentation] 視覚の適応と婚姻色の進化を介した生殖的隔離が引き起こした種の多様性 (2007)
  • Author(s): 寺井洋平、宮城竜太郎、溝入真治, 相原光人、Semvua I. Mzighani、岡田典弘
  • Organizer: 日本進化学会 第9回大会
  • Place of Presentation: 京都・京都大学
  • Year and Date: 2007-09-01
• [Presentation] The diversity of opsins in sympatric Lake Victoria cichlid species (2007)
  • Author(s): Ryutaro Miygai, Yohey Terai, Mitsuto Aibara, Shinji Mizoiri and Norihiro Okada
  • Organizer: Evolution 2007
  • Place of Presentation: New Zealand Christchurch
  • Year and Date: 2007-06-17
• [Presentation] A new approach to resolve the relationship among the closely related cichlid species in Lake Victoria (2007)
  • Author(s): Naoki Kobayashi, Masakatsu Watanabe, Jun-ichi Asakawa, and Norihiro Okada
  • Organizer: Evolution meeting
  • Place of Presentation: New Zealand Christchurch
  • Year and Date: 2007-06-17
• [Presentation] Adaptation of opsins causes the species diversity of Lake Victoria cichlid fishes (2007)
  • Author(s): Yohey Terai, Sinji Mizoiri, Mitsuto Aibara, Ryutaro Miyagi, and Norihiro Okada
  • Organizer: Evolution 2007
  • Place of Presentation: New Zealand Christchurch
  • Year and Date: 2007-06-17
• [Presentation] カワスズメ科魚類の頭部形態形成のための発生段階 (2007)
  • Author(s): 藤村衡至, 岡田典弘
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福山・福岡国際会議場
  • Year and Date: 2007-05-30
• [Presentation] The developmental basis for craniofacial morphology of cichlid fishes (2007)
  • Author(s): Fuj imura K, Okada N.
  • Organizer: Asia Africa Evolution Meeting (JSPS AA Science Pl atform Program)
  • Place of Presentation: 千葉・生命の森リゾート
• [Presentation] Expression analysis of cichlid hagoromo gene (2007)
  • Author(s): Kensuke Tashiro
  • Organizer: Asia Africa Evolution Meeting
  • Place of Presentation: 千葉・生命め森リゾート
• [Presentation] Nrf2活性モニター用トランスジェニックゼブラフィッシュ系の開発 (2007)
  • Author(s): 辻田忠志, 小林麻己人, 川上弘一, 山本雅之
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] トランスポゾンを用いたGal4-UAS法による細胞の可視化と機能改変 (2007)
  • Author(s): Kawakami, K., Abe, G., Urasaki, A., Kikuta, H., Kishimoto, Y., Mutou, A., Maximiliano, S.,
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 遺伝子トラップ系統を用いたゼブラフィッシュ三半規管形態形成の解析 (2007)
  • Author(s): 舟橋淳一, 川上浩一, 仲村春和
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Transposon-mediated gene trapping and enhancer trapping in zebrafish (2007)
  • Author(s): Kawakami, K.
  • Organizer: ISDB
  • Place of Presentation: Agra, India
• [Presentation] Transposon-mediated gene and enhancer trapping in zebrafish (2007)
  • Author(s): Kawakami, K.
  • Organizer: The 16th Korea Genome Organization Conference
  • Place of Presentation: Seoul
• [Presentation] 三次元タイムラプスによるゼブラフィッシュ三半規管形態形成の解析 (2007)
  • Author(s): 舟橋淳一, 川上浩一, 仲村春和
  • Organizer: 第13回小型魚類研究会
  • Place of Presentation: 東京
• [Presentation] .魚類の外部器官であるMitochondria-rich cells (MRCs) の複雑な発生分化機構 (2007)
  • Author(s): 江嵜正浩, 星島一幸, 川上浩一, Eric S. Weinberg, 広瀬茂久
  • Organizer: 第13回小型魚類研究
  • Place of Presentation: 東京
• [Presentation] Insertion of RFP using gene trap results in a knockdown of the pax8 gene (2007)
  • Author(s): Gebhart, N., Kawakami, K., Ono, F.
  • Organizer: 5th European Zebrafish Genetics and Development Meeting
  • Place of Presentation: Amsterdam
• [Presentation] Visualization In zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3A (2007)
  • Author(s): Esaki, M., Hoshijima, K., Kobaysshi, S., fukuda, H., Kawakami, K., Hirose, S.
  • Organizer: 5th European Zebrafish Genetics and Development Meeting
  • Place of Presentation: Amsterdam
• [Presentation] Analysis of conserved element regulating expression of fgf8 in zebrafish (2007)
  • Author(s): Komisarczuk, A., Navaratilova, P., Kawakami, K., Becker, T.
  • Organizer: 5th European Zebrafish Genetics and Development Meeting
  • Place of Presentation: Amsterdam
• [Presentation] Targeted gene expression by GAL4 gene and enhancer trapping in zebrafish defines subsets of neurons required for simple vetebrate behaviors (2007)
  • Author(s): Asakawa, K., Mizusawa, K., Nagayoshi, S., Kotani, T., Urasaki, A., Kishimoto, Y., Hibi, M., Kawakami, K.
  • Organizer: 5th European Zebrafish Genetics and Development Meeting
  • Place of Presentation: Amsterdam
• [Presentation] New genetic techniques by excision of the To12 transposon: isolation of revertants and creating frame shift mutations (2007)
  • Author(s): Urasaki, A., Asakawa, K., Kawakami, K.
  • Organizer: 5th European Zebrafish Genetics and Development Meeting
  • Place of Presentation: Amsterdam
• [Presentation] Somitic contribution to the formation of dorsal aorta involves cell migration regulated by Notch and ephrin (2007)
  • Author(s): Yoshiko Takahashi, Emi Ohata, Tadayoshi Watanabe, Teruaki Takahashi, Koichi Kawakami, Jun Kohyama, Hideyuki Okano, Yuki Sato
  • Organizer: 第40回日本発生生物学会 第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡
• [Presentation] ゼブラフィッシュGAL4トラップ法を用いた行動制御に関与する神経細胞の探索 (2007)
  • Author(s): 浅川和秀, 水澤寛太, 永吉さおり, 小谷友也, 浦崎明宏, 岸本康之, 日比正彦, 川上浩一
  • Organizer: 第40回日本発生生物学会 第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡
• [Presentation] ゼプラフィッシュにおけるJump starterトランスポゾンシステムの開発 (2007)
  • Author(s): 浦崎明宏, 浅川和秀, 川上浩一
  • Organizer: 第40回日本発生生物学会 第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡
• [Presentation] ゼブイッシュ幼生におけるNaイオン吸収細胞の同定とその発生分化機構の解析 (2007)
  • Author(s): 江崎正浩, 星島一幸, 小林さや子, 福田英一, 川上浩一, 広瀬茂久
  • Organizer: 第40回日本発生生物学会 第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡
• [Presentation] :遺伝子トラップ系統を用いたゼブラフィッシュ三半規管形態形成の解析 (2007)
  • Author(s): 舟橋淳一, 川上浩一, 仲村春和
  • Organizer: 第40回日本発生生物学会 第59回日本細胞生物学会 合同大会
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://www.evolution.bio.titech.ac.jp/index0.html
• [Remarks]
  • URL: http://kawakami.lab.nig.ac.jp/
• [Publications] Terai Y., Takahashi K., Nishida M., Sato T., Okada N.: "Using SINEs to Probe Ancient Explosive Speciation : "Hidden" Radiation of African Cichlids?"Mol.Biol.Evol.. 20. 924-930 (2003)
• [Publications] Watanabe M., Kobayashi N., Fujiyama A., Okada N.: "Construction of a BAC library for Haplochromis chilotes, a cichlid fish from Lake Victoria."Genes Genet Syst. 78(1). 103-105 (2003)
• [Publications] Terai Y., Morikawa N., Kawakami K., Okada, N.: "The complexity of alternative splicing of hagoromo mRNA is increased in an explosively speciated lineage in east African cichlids."Proc.Natl.Acad.Sci.USA. 100. 12798-12803 (2003)
• [Publications] Terai Y., Takezaki N., Mayer W.E., Tichy H., Takahata N., Klein J., Okada, N.: "Phylogenetic relationships among East African Haplochromine fish as revealed by short interspersed elements (SINEs)."J.Mol.Evol.. 58. 64-78 (2004)
• [Publications] Terai Y, Morikawa N, Kawakami K, Okada N.: "Accelerated Evolution of the Surface Amino Acids in the WD-Repeat Domain Encoded by the hagoromo Gene in an Explosively Speciated Lineage of East African Cichlid Fishes"Molecular Biology and Evolution. 19. 1303-1312 (2002)
• [Publications] Terai Y, Morikawa N, Okada N.: "The evolution of the pro-domain of bone morphogenetic protein 4 (bmp4) in an explosively speciated lineage of East african cichlid fishes"Molecular Biology and Evolution. 19. 1628-1632 (2002)
• [Publications] Sugawara T, Terai Y, Okada N.: "Natural selection of the rhodopsin gene during the adaptive radiation of East african great lakes cichlid fishes"Molecular Biology and Evolution. 19. 1807-1811 (2002)
• [Publications] Takahashi, K., Okada, N.: "Mosaic structure and retrotranspositional dynamics during evolution of subfamilies of short interspersed elements in African cichlids"Molecular Biology and Evolution. 19. 1303-1312 (2002)
• [Publications] Terai Y, Mayer WE, Klein J, Tichy H, Okada N.: "The effect of selection on a long wavelength-sensitive (LWS) opsin gene of Lake Victoria cichlid fishes"Proceedings of the National Academy of Sciences of the United States of America. 99. 15501-15506 (2002)
• [Publications] Terai, Y., Takahashi, K., Okada, N.: "SINEs as probes for ancient explosion of speciation-A "hidden" adaptive radiation of African"Molecular Biology and Evolution. (印刷中). (2003)