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時間分解紫外共鳴ラマン法によるセンサー蛋白の情報感知・伝達及び機能発現機構の解明

Research Project

Project/Area Number 14103003
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Physical chemistry
Research InstitutionOkazaki National Research Institutes

Principal Investigator

北川 禎三  岡崎国立共同研究機構, 統合バイオサイエンスセンター, 教授 (40029955)

Co-Investigator(Kenkyū-buntansha) 内田 毅  岡崎国立共同研究機構, 分子科学研究所, 助手 (30343742)
Project Period (FY) 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥67,340,000 (Direct Cost: ¥51,800,000、Indirect Cost: ¥15,540,000)
Fiscal Year 2002: ¥67,340,000 (Direct Cost: ¥51,800,000、Indirect Cost: ¥15,540,000)
Keywords酸素センサー蛋白 / ヘム蛋白質 / 共鳴ラマン分光 / HemAT / Dos / 鉄-酸素伸縮振動
Research Abstract

本研究の実施前に辞退する事になったため本格的な実施ではないが、特別推進研究においても関連課題を実施しているので成果の一部をここに記す。
バシラスという細菌の酵素分子センサーであるHemATという蛋白の共鳴ラマンスペクトルを測定し、酸素分子の特異的認識メカニズムを構造化学的に調べた。すなわち、HemATの鉄-酸素伸縮振動を同位体置換法で帰属し、この振動数が非常に低い事から、鉄に結合した酸素がまわりの蛋白と強く水素結合していると結論した。シグナル伝達部の長さを変えると、強く水素結合した酸素とそうでない酸素の存在比が変化したので、その水素結合が情報伝達に寄与していると考えられた。

Report

(1 results)
  • 2002 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] T.Kitagawa, N.Haruta, Y.Mizutani: "Time-resolved resonance Raman study on ultrafast structural relaxation and vibrational cooling of photodissociated carbonmonoxy myoglobin"Biopolymers (Biospectroscopy). 67. 207-213 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Aki, T.Ogura, Y.Naruta, T.H.Le, T.Sato, T.Kitagawa: "UV resonance Raman characterization of model compounds of Tyr244 of Bovine cytochrome c oxidase in its neutral-, deprotonated anion-, and deprotonated neutral radical-forms ; Effects of covalent binding between tyrosine and histidine"Journal of Physical Chemistry. 106. 3436-3444 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Aono, T.Kato, M.Matsuki, H.Nakajima, T.Ohta, T.Uchida, T.Kitagawa: "Resonance Raman and ligand binding studies of the oxygen sensing signal transducer protein HemAT from Bacillus subtilis"Journal of Biological Chemistry. 277. 13528-13538 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.Haruta, T.Kitagawa: "Time-resolved UV resonance Raman investigation of protein folding using a rapid mixer : Characterization of kinetic folding intermediates of apomyoglobin"Biochemistry. 41. 6595-6604 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Mizatani, T.Kitagawa: "Vibrational energy relaxation of metalloporphyrins in a condensed phase probed by time-resolved resonance Raman spectroscopy"Bulletin of the Chemical Society of Japan. 75. 623-639 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Sato, Y.Sasakura, S.Sugiyama, I.Sagami, T.Shimizu, Y.Mizutani, T.Kitagawa: "Stationary and time-resolved resonance Raman spectra of His77 and Met95 mutants of the isolated heme domain of a direct oxygen sensor from Escherichia coli"Journal of Biological Chemistry. 277. 32650-32658 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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