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Molecular mechanism of multiple dissimilatory nitrogen metabolisms by eukaryotic and prokaryotic cells

Research Project

Project/Area Number 14104005
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

SHOUN Hirofumi  The University of Tokyo, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, PROFESSOR (70012036)

Project Period (FY) 2002 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥114,010,000 (Direct Cost: ¥87,700,000、Indirect Cost: ¥26,310,000)
Fiscal Year 2006: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2005: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2004: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2003: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2002: ¥37,960,000 (Direct Cost: ¥29,200,000、Indirect Cost: ¥8,760,000)
Keywordsfungal denitrification / copper-containing nitrite reducatse / cytochrome P450nor / ammonia fermentation / Fusarium oxysporum / codenitrification / anaerobic respiration by actinomycetes / aerobic denitrifying bacteria / 脱窒真菌 / 亜硝酸塩還元酵素 / 一酸化窒素還元酵素 / フラボヘモグロビン / 好気脱窒 / 窒素固定菌 / 脱窒 / 複合呼吸 / 硝酸塩還元酵素 / ギ酸脱水素酵素 / ミトコンドリア / P450nor / NADH / 放線菌
Research Abstract

(1) Molecular mechanisms of fungal denitrification system: The genes encoding copper-containing nitrite reductase (nirK) were isolated from several fungi including Fusarium oxysporum, in which fungal denitrifying activity was first found. The NirK protein previously isolated from F. oxysporum, was shown to be the product of the nirK gene. This is the first isolation from eukaryote of an orthologoue of the bacterial counterpart involved in denitrification. Phylogenetic analysis shows that these nirK genes and their homologue genes that have been recently found in many eukaryotic microorganisms are originated from the single ancestor, suggesting that they are derived from the protomitochondrion. It was shown that the nitrate reducing system of F. oxysporum comprises nitrate reductase (dNaR, possibly NarGHI, which is universally found in bacterial nitrate respiration) and ubiquinone-dependent formate dehydrogenase. Unlike the bacterial system the fungal dNaR system was shown to function s … More imultaneously with cytochrome oxidase under micro-aerobic conditions, which we termed hybrid respiration. These results have revealed general occurrence of fungal denitrification in nature.
(2) Reaction mechanism of cytochrome P450nor: P450nor participates in fungal denitrification as nitric oxide (NO) reductase, performing an unusual electron transfer, direct transfer of 2 electrons from NADH to the hemeprotein. Structural and kinetic analyses such as determination of the crystal structure of P450nor in complex with an NAD analogue revealed-the detailed reaction mechanism of P450nor, including the mechanism of the prochiral hydride transfer from NADH to the ferric heme-NO complex and identification of the chemical entity of the key spectral intermediate. The results should have gave an marked impact, to many field such as bioinorganic chemistry and P450 biochemistry.
(3) Ammonia fermentation and anaerobic growth of fungi: Involvement of the assimilatory nitrate reducing system in the fungal ammonia fermentation was confirmed by characterizing the uiaD or niiA-defective mutant of the fungus Aspergillus nidulans. The denitrifying fungus F. oxysporum was shown to perform hetero-lactic acid fermentation, which is coupled to ammonia fermentation. The results together with those of denitrification show that al least a portion of soil fungi are facultative with respect to aeration, not obligate aerobe as previously thought.
(4) Other subjects: Codenitrification was shown to be a biological reaction but not chemical reaction, which was supported by the results of its saturation kinetics against codenitrification substrates, and inhibition by respiration inhibitors such as cyanide. Actinomycetes are thought to be obligate aerobes, By contrast, we provided evidence for occurrence of nitrate respiration in the actinomycetes Streptomyces antibioticus and Streptomyces coelicolor. Aerobic denitrifying bacteria were isolated in order to apply them to the control of green house-effect gas emission, and their properties were characterized. Less

Report

(6 results)
  • 2007 Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (116 results)

All 2008 2007 2006 2005 2004 2003 2002 Other

All Journal Article (76 results) (of which Peer Reviewed: 29 results) Presentation (26 results) Book (3 results) Publications (11 results)

  • [Journal Article] Streptomyces griseus enhances denitrification by Ralstonia pickettii K50, which is possibly mediated by histidine produced during CO-culture2008

    • Author(s)
      Takaki, S. Fushinobu, S. W. Kim, M. Miyahara, T. Wakagi, H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem 72(1)

      Pages: 163-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] P450 norの生理機能、反応機構、構造2008

    • Author(s)
      祥雲弘文, 伏信進矢
    • Journal Title

      生化学 80(6)

      Pages: 560-568

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Streptomyces griseus enhances denitrification by Ralstonia pickettii K50, which is possibly mediated by histidine produced during co-culture2008

    • Author(s)
      Takaki, S. Fushinobu, S.-W. Kim, M. Miyahara, T. Wakagi, H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem. 72(1)

      Pages: 163-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] シトクロムP450とペルオキシゲナーゼ反応2006

    • Author(s)
      祥雲弘文
    • Journal Title

      ファインケミカル 35

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ミトコンドリア嫌気呼吸とカビの通性嫌気性2006

    • Author(s)
      祥雲弘文
    • Journal Title

      蛋白質・核酸・酵素 51(5)

      Pages: 419-429

    • NAID

      40007273472

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov.2006

    • Author(s)
      K. Hatayama, H. Shoun, Y. Ueda, A. Nakamura
    • Journal Title

      Int. J. Syst. Evol. Microbiol. 56(11)

      Pages: 2545-2551

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Denitrification by the mix-culturing of fungi and bacteria with shell2006

    • Author(s)
      D. Liu, S. Zhang, Y. Zheng, H. Shoun
    • Journal Title

      Microbiol Res. 161(2)

      Pages: 132-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cytochrome P450 and peroxygenase reaction2006

    • Author(s)
      Shoun, H.
    • Journal Title

      Fine Chemical 35

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Anaerobic respiration by mitochondria and under anaerobic characteristics of fungi2006

    • Author(s)
      Shoun, H.
    • Journal Title

      PNE 51 (5)

      Pages: 419-429

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb, nov.2006

    • Author(s)
      K. Hatayama, H. Shoun, Y. Ueda, A. Nakamura.
    • Journal Title

      Int. J. Syst. Evol. Microbiol. 56(11)

      Pages: 2545-2551

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Denitrification by the mixculturing of fungi and bacteria with shell2006

    • Author(s)
      D. Liu, S. Zhang, Y. Zheng, H. Shoun
    • Journal Title

      Microbiol Res. 161(2)

      Pages: 132-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] シトクロムP450とペルオキシゲナーゼ反応2006

    • Author(s)
      祥雲弘文
    • Journal Title

      ファインケミカル 35(3)

      Pages: 49-54

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 低酸素環境下での呼吸と発酵2005

    • Author(s)
      高谷直樹, 祥雲弘文
    • Journal Title

      バイオサイエンスとインダストリー 63(4)

      Pages: 233-236

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isol ated from a compost pile2005

    • Author(s)
      K. Hatayama, S. Kawai, H. Shoun, Y. Ueda, A. Nakamura
    • Journal Title

      Int J Syst Evol Microbiol. . 55(4)

      Pages: 1539-1544

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family 'Thermoactinomycetaceae'isolated from compost2005

    • Author(s)
      K. Hatayama, H. Shoun, Y. Ueda, A. Nakamura
    • Journal Title

      Int J Syst Evol Microbiol. 55(5)

      Pages: 2101-2104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitric oxide reductase (P450nor)from Fusarium oxyaporum2005

    • Author(s)
      Andreas Daiber, Hirofumi Shoun, Volker Ullrich
    • Journal Title

      Inorganic Biochemistry 99

      Pages: 185-193

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Breathing under the hypoxia environment and fermentation2005

    • Author(s)
      N. Takaya, Shoun, H.
    • Journal Title

      Bioscience and bioindustry 63(4)

      Pages: 233-236

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile2005

    • Author(s)
      K. Hatayama, S. Kawai, H. Shoun, Y. Ueda, A. Nakamura
    • Journal Title

      Int J Syst Evol Microbiol. 55(4)

      Pages: 1539-1544

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Planifilum fimeticola gen. nov., sp. nov. and. Planifilum fulgidum sp. nov., novel members of the family Thermoactinomycetaceae' isolated from compost2005

    • Author(s)
      K. Hatayama, H. Shoun, Y. Ueda, A. Nakamura
    • Journal Title

      Int J Syst Evol Microbiol. 55(5)

      Pages: 2101-2104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitric oxide reductase (P450nor) from Fusarium oxyaporum2005

    • Author(s)
      Andreas Daiber, Hirofumi Shoun, Volker Ullrich
    • Journal Title

      Inorganic Biochemistry

      Pages: 185-193

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Planifilumfimeticola gen.nov., sp.nov. and Planifilumfulgidum sp.nov., novel members of the family 'Thermoactinomycetaceae'isolated from compost2005

    • Author(s)
      K.Hatayama, H.Shoun, Y.Ueda, A.Nakamura
    • Journal Title

      Int J Syst Evol Microbiol. 55(5)

      Pages: 2101-2104

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pseudomonas azotifigens sp.nov., a novel nitrogen-fixing bacterium isolated from a compost pile2005

    • Author(s)
      K.Hatayama.S.Kawai, H.Shoun, Y.Ueda, A.Nakamura
    • Journal Title

      Int J Syst Evol Microbiol. 55(4)

      Pages: 1539-1544

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide reductase (P450(nor)) from Fusarium oxysporum2005

    • Author(s)
      A.Daiber, H.Shoun, V.Ullrich
    • Journal Title

      J.Inorg.Biochem. 99(1)

      Pages: 185-193

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anaerobic respiration and fermentation by filamentous fungi2005

    • Author(s)
      N.Takaya, H.Shoun
    • Journal Title

      Bioscience and Industry 63(4)

      Pages: 233-236

    • NAID

      10015464881

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 微生物の新規窒素代謝の発見とその解明(2004年度日本農芸化学会学会賞受賞総説)2004

    • Author(s)
      祥雲弘文
    • Journal Title

      日本農芸化学会誌 78 (8)

      Pages: 724-729

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.2004

    • Author(s)
      Oshima, R. . Fushinobu, S., Su, F., Zhang, L., Takaya, N., Shoun, H
    • Journal Title

      J. Mol. Biol 342

      Pages: 207-217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] D88A mutant of cytochrome P450nor provides kinetic evidence for directcomplex formation with electron donor NADH2004

    • Author(s)
      M. Umemura, F. Su, N. Takaya, Y. Shiro, H. Shoun
    • Journal Title

      Eur. J. Biochem. . 271(14)

      Pages: 2887-2894

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Isolation of flavohemoglobin from the Actinomycete Streptomyces antibioticus grown without external nitric oxide stress2004

    • Author(s)
      Y. Sasaki, N. Takaya, A. Nakamura, H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem. 68(5)

      Pages: 1106-1112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitrous oxide-forming codenitrification catalyzed by cytochrome P450nor2004

    • Author(s)
      F. Su, N. Takaya., H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem. 68(2)

      Pages: 473-475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of a Glu71・Arg64 couple in the access channel for NADH in cytochrome P450nor2004

    • Author(s)
      F. Su, S. Fushinobu, N. Takaya, H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem. 68(5)

      Pages: 1156-1159

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Unusual transcription regulation of the niaD gene under anaerobic conditions supporting fungal ammonia fermentation2004

    • Author(s)
      K. Takasaki, H. Shoun, A. Nakamura, T. Hoshino, N. Takaya
    • Journal Title

      Biosci Biotechnol Biochem 68(4)

      Pages: 978-980

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Fungal ammonia fermentation - A novel metabolic mechanism that couples the dissimilatory and assimilatory pathways of both nitrate and ethanol -Role of acetyl CoA synthetase in anaerobic ATP synthesis2004

    • Author(s)
      K. Takasaki, H. Shoun, M. Yamaguchi, K. Takeo, A. Nakamura, T. Hoshino, N. Takaya
    • Journal Title

      J. Biol. Chem. 279

      Pages: 12414-12420

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Discovery and Elucidation of Novel Microbial Metabolism2004

    • Author(s)
      Shoun, H.
    • Journal Title

      JSBBA 78(8)

      Pages: 724-729

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Structural evidence for direct hydride transfer from NADH to cytochrome P450nor2004

    • Author(s)
      R. Oshima, S. Fushinobu, F. Su, L. Zhang, N. Takaya, H. Shoun
    • Journal Title

      J. Mol. Biol. 342(1)

      Pages: 207-217

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] D88A mutant of cytochrome P450nor provides kinetic evidence for direct complex formation with electron donor NADH2004

    • Author(s)
      M. Umemura, F. Su, N. Takaya, Y. Shiro, H. Shoun
    • Journal Title

      Eur. J. Biochem. 271(14)

      Pages: 2887-2894

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitrous oxide-forming codenitrification catalyzed by cytochrome P450nor2004

    • Author(s)
      F. Su, N. Takaya, H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem. 68 (2)

      Pages: 473-475

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of a Glu71-Arg64 couple in the access channel for NADH in cytochrome P450nor2004

    • Author(s)
      F. Su, S. Fushinobu, N. Takaya, H. Shoun
    • Journal Title

      Biosci. Biotechnol. Biochem. 68 (5)

      Pages: 1156-1159

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Unusual transcription regulation of the niaD gene under anaerobic conditions supporting fungal ammonia fermentation2004

    • Author(s)
      K. Takasaki, H. Shoun, A. Nakamura, T. Hoshino, N Takaya
    • Journal Title

      Biosci Biotechnol Biochem. 68 (4)

      Pages: 978-980

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fungal ammonia fermentation - A novel metabolic mechanism that couples the dissimilatory and assimilatory pathways of both nitrate and ethanol -Role of acetyl CoA synthetase in anaerobic ATP synthesis2004

    • Author(s)
      K. Takasaki, H. Shoun, M. Yamaguchi, K. Takeo, A. Nakamura, T. Hoshino, N. Takaya
    • Journal Title

      J. Biol. Chem. 279(13)

      Pages: 12414-12420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fungai ammonia fermentation - A novel metabolic mechanism that couples the dissimilatory and assimilatory pathways of both nitrate and ethanol - Role of acetyl CoA synthetase in anaerobic ATP synthesis2004

    • Author(s)
      K.Takasaki, H.Shoun, M.Yamaguchi, K.Takeo, A.Nakamura, T.Hoshino, N.Takaya
    • Journal Title

      J.Biol.Chem 279(13)

      Pages: 1087-1089

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Unusual transcription regulation of the niaD gene under anaerobic conditions supporting fungal ammonia fermentation2004

    • Author(s)
      K.Takasaki, H.Shoun, A.Nakamura, T.Hoshino, N.Takaya
    • Journal Title

      Biosci Biotechnol Biochem 68(2)

      Pages: 473-475

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation of flavohemoglobin from the Actinomycete Streptomyces antibioticus grown without external nitric oxide stress2004

    • Author(s)
      Y.Sasaki, N.Takaya, A.Nakamura, H.Shoun
    • Journal Title

      Biosci.Biotechnol.Biochem. 68(5)

      Pages: 1106-1112

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Involvement of a Glu71-Arg64 couple in the access channel for NADH in cytochrome P450nor2004

    • Author(s)
      F.Su, S.Fushinobu, N.Takaya, H.Shoun
    • Journal Title

      Biosci.Biotechnol.Biochem 68(5)

      Pages: 1156-1159

    • Related Report
      2004 Annual Research Report
  • [Journal Article] D88A mutant of cytochrome P450nor provides kinetic evidence for direct complex formation with electron donor NADH2004

    • Author(s)
      M.Umemura, F.Su, N.Takaya, Y.Shiro, H.Shoun
    • Journal Title

      Eur.J.Biochem 271(14)

      Pages: 2887-2894

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structural evidence for direct hydride transfer from NADH to cytochrome P450nor2004

    • Author(s)
      R.Oshima, S.Fushinobu, F.Su, L.Zhang, N.Takaya, H.Shoun
    • Journal Title

      J.Mol.Biol 342(1)

      Pages: 207-217

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cloning and enhanced expression of the cytochrome P450nor gene (nicA ; CYP55A5) encoding nitric oxide reductase from Aspergillus oryzae2004

    • Author(s)
      N.Takaya, H.Shoun
    • Journal Title

      Biosci.Biotechnol.Biochem 68(10)

      Pages: 2040-2049

    • Related Report
      2004 Annual Research Report
  • [Journal Article] カビのアンモニア発酵 : 土壌真菌は通気条件の変化に応じてエネルギー代謝を変える通性嫌気性生物2003

    • Author(s)
      周哲敏, 祥雲弘文
    • Journal Title

      化学と生物 41

      Pages: 561-562

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 真菌脱窒系の発見とP450norの構造・機能相関解明2003

    • Author(s)
      祥雲弘文
    • Journal Title

      バイオサイエンスとインダストリー. 61

      Pages: 11-16

    • NAID

      10010181518

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Aerobic denitrifying bacteria that produce low levels of nitrous oxide.2003

    • Author(s)
      Takaya, N., Catalan, Sakairi, M. A. B., Sakaguchi, Y., Kato, I., Zhou, Z., Shoun, H
    • Journal Title

      Appl. Environ. Microbiol. . 69(6)

      Pages: 3152-3157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Denitrification of nitrate by the fungus Cylindrocarpon tonkinense2003

    • Author(s)
      Watsuji, T., Takaya, N., Nakamura, A., Shoun, H.
    • Journal Title

      Biosci. Biotech. Biochem 67

      Pages: 1115-1120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A Possible Role of NADPH-dependent Cytochrome P450nor Isozyme in Glycolysis Under Denitrifying Conditions2003

    • Author(s)
      Watsuji, T., Takaya, N., Nakamura, A., Shoun, H.
    • Journal Title

      Biosci. Biotech. Biochem. . 67

      Pages: 1109-1114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Formate-forming fungal catabolic pathway to supply electrons to nitrate respiration.2003

    • Author(s)
      Kuwazaki, S., Takaya, N., Nakamura, A., Shoun, H.
    • Journal Title

      Biosci. Biotech. Biochem. 67

      Pages: 937-939

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Hybrid respiration in the denitrifying mitochondria of Fusarium oxysporum. .2003

    • Author(s)
      Takaya, N., Kuwazaki, S., Adachi, Y., Suzuki, S., Kikuchi, T., Nakamura, H., Shiro, Y., Shoun H.
    • Journal Title

      J. Biochem. 133

      Pages: 461-465

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Ammonia fermentation of the fungi :2003

    • Author(s)
      Zhou, Z . Shoun, H.
    • Journal Title

      KAGAKU TO SEIBUTSU 41

      Pages: 561-562

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Dicovery of the Fungal Denitrifying System and Studies on Structure-Function Relationship of P450nor2003

    • Author(s)
      Shoun, H.
    • Journal Title

      Bioscience and bioindustry 61

      Pages: 11-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Aerobic denitrifying bacteria that produce low levels of nitrous oxide.2003

    • Author(s)
      Takaya, N., Catalan-Sakairi, M. A. B., Sakaguchi, Y., Kato, I., Zhou, Z., Shoun, H.
    • Journal Title

      Appl. Environ. Microbiol. 69(6)

      Pages: 3152-315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Denitrification of nitrate by the fungus Cylindrocarpon tonkinense2003

    • Author(s)
      Watsuji, T., Takaya, N., Nakamura, A., Shoun, H.
    • Journal Title

      Biosci. Biotech. Biochem. 67

      Pages: 1115-1120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A Possible Role of NADPH-dependent Cytochrome P450nor Isozyme in Glycolysis Under Denitrifying Conditions2003

    • Author(s)
      Watsuji, T., Takaya, N., Nakamura, A., Shoun, H.
    • Journal Title

      Biosci. Biotech. Biochem. 67

      Pages: 1109-1114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Formate-forming fungal catabolic pathway to supply electrons to nitrate respiration2003

    • Author(s)
      Kuwazaki, S., Takaya, N., Nakamura, A., Shoun, H.
    • Journal Title

      Biosci. Biotech. Biochem. 67

      Pages: 937-939

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Hybrid respiration in the denitrifying mitochondria of Fusarium oxysporum.2003

    • Author(s)
      Takaya, N., Kuwazaki, S., Adachi, Y., Suzuki, S., Kikuchi, T., Nakamura, H., Shiro, Y., Shoun H.
    • Journal Title

      J. Biochem. 133

      Pages: 461-465

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] カビと放線菌の新規窒素呼吸系.2002

    • Author(s)
      祥雲弘文
    • Journal Title

      バイオサイエンスとインダストリー 60(7)

      Pages: 439-443

    • NAID

      10009280808

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 低酸素状態でのカビの生き残り戦略 : わかってきたカビ脱窒系の役割. 呼吸系の進化や窒素サイクルを考えるヒントに2002

    • Author(s)
      高谷直樹, 祥雲弘文
    • Journal Title

      化学と生物 40(6)

      Pages: 356-358

    • NAID

      10008566170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetic engineering using the fungal flavohemoglobin for constructing the Pseudomonas stutzeri strain that produces less nitrous oxide duringdenitrification.2002

    • Author(s)
      Takaya, N., Shoun, H.
    • Journal Title

      J. Biosci. Bioeng. 94

      Pages: 282-284

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Novel nitrogen respiration by fungi and actinomycetes2002

    • Author(s)
      Shoun, H.
    • Journal Title

      Bioscience and bioindustry 60(7)

      Pages: 439-443

    • NAID

      10009280808

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A survival strategy of the mold in the hypoxia state : Gradually known a role of denitrificatio:by fungi, For a hint to think about evolution and the nitrogen cycle of the respiratory system2002

    • Author(s)
      N. Takaya, Shoun, H.
    • Journal Title

      KAGAKU TO SEIBUTSU 40(6)

      Pages: 356-358

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetic engineering using the fungal flavohemoglobin for constructing the Pseudomonas stutzeri strain that produces less nitrous oxide during denitrification.2002

    • Author(s)
      Takaya, N., Shoun, H.
    • Journal Title

      J. Biosci. Bioeng. 94

      Pages: 282-284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A physiology, reaction mechanism and structure of P450nor

    • Author(s)
      Shoun, H., S. Pushinobu
    • Journal Title

      SEIKAGAKU (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Eukaryotic nirK genes encoding copper-containing nitrite reductase originated from the protomitochondrion.

    • Author(s)
      Kim, S-W, Fushinobu, S, Wakagi, T, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Copper-containing nitrite reductase gene of the fungus Aspergillus oryzae.

    • Author(s)
      Nakanishi, Y, Zhou, S, Kim, S-W, Fushinobu, S, Wakagi, T, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Flavohemoglobin isozymes of the fungus Aspergillus oryzae.

    • Author(s)
      Zhou, S, Kim, S-W, Fushinobu, S, Wakagi, T, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Hetero-lactic acid fermentation by the fungus Fusarium oxysporum coupled to ammonia fermentation.

    • Author(s)
      Zhou, Z, Takaya, N, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mineralization of organic nitrogen by Streptomyces antibioticus forming ' nitrite and ammonia.

    • Author(s)
      Sasaki, Y, Hirabayashi, K, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Codenitrification by the fungus Fusarium solani.

    • Author(s)
      Jian, R, Kim, D-H, Takaya, N, Fushinobu, S, Wakagi, T, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Anion hole in cytochrome P450nor.

    • Author(s)
      Su, F., Fushinobu, S, Wakagi, T, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] NADH-peroxidase activity catalyzed by cytochrome P450nor.

    • Author(s)
      Kurose, T, Ozaki, M, Nakaya, S, Fushinobu, S, Wakagi, T, Shoun, H
    • Journal Title

      (In preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Streptomyces griseus enhances denitrification by Ralstonia pickettii K50, which is possibly mediated by histidine produced during the co-culture.

    • Author(s)
      Takaki, K. Fushinobu, S. Kim, S. Miyahara, M. Wakagi, T. and Shoun, H.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry (印刷中)

    • Related Report
      2006 Annual Research Report
    • Peer Reviewed
  • [Presentation] P450foxyの基質特異性に関する研究2008

    • Author(s)
      小川徳之, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 糸状菌Aspergillus oryzaeにおけるflavohemoglobinの機能解析2008

    • Author(s)
      周勝敏, 中西義人, 金尚完, 伏信進矢, 若木高善, 祥雲弘
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] P450norのNADH・ペルオキシダーゼ活性のph依存性と速度論的解析2008

    • Author(s)
      黒瀬大造, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 放線菌Streptomyces avermitilis由来のCYP105P1およびCYP105D6の結晶構造2008

    • Author(s)
      許蓮花, 伏信進矢, 若木高善, 池田治夫, 祥雲弘文
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] P450s and P450 foxy, two fungal P450 species containing unique electron transport systems2007

    • Author(s)
      祥雲 弘文
    • Organizer
      15th International Conference on Cytochromes P450 〜Biochemistry, Biophysics, Functional genomics〜
    • Place of Presentation
      スロベニア
    • Year and Date
      2007-06-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 放線菌およびカビの嫌気呼吸と窒素異化代謝2007

    • Author(s)
      祥雲 弘文
    • Organizer
      2007年度(第22回)日本放線菌学会大会
    • Place of Presentation
      広島
    • Year and Date
      2007-05-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] カビ脱窒系の解明とシトクロムP450nor2007

    • Author(s)
      祥雲 弘文
    • Organizer
      第7回日本NO学会学術集会
    • Place of Presentation
      滋賀
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 好気脱窒菌Ralstonia pickettii K50株の脱窒に対する外部因子の影響2007

    • Author(s)
      高木恒輝, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Direct Hydride Transfer from NADH to Cytochrome P450nor2006

    • Author(s)
      祥雲 弘文
    • Organizer
      The 50th Anniversary of Oxygenases : Advances and Reflections縲怐u酵素添加酵素」の50年-展望と回顧-
    • Place of Presentation
      東京
    • Year and Date
      2006-04-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Aspergillus oryzaeのflavohemoglobinの解析2006

    • Author(s)
      周勝敏, 中西義人, 金尚完, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2006年度大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 酵母の脱窒系の解明2006

    • Author(s)
      太田匠吾, 高木恒輝, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2006年度大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Ecological Aspects of Denitrification, with Emphasis on Agriculture2004

    • Author(s)
      祥雲 弘文
    • Organizer
      COST Action 856
    • Place of Presentation
      ドイツ
    • Year and Date
      2004-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 麹菌Aspergillus oryzaeの異化型亜硝酸還元酵素(Nir)遺伝子の単離とその解析2004

    • Author(s)
      中西義人, 金尚完, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] カビCylindrocarpon tonkinenseの脱窒系の解明2004

    • Author(s)
      金尚完, 中西義人, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 酵母Candida colliculosaとFellomyces fuzhouensisの脱窒系の解明2004

    • Author(s)
      辻真博, 中西義人, 金尚完, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 放線菌Streptomyces avermitilisの一酸化窒素合成酵素(Nos)の生理機能2004

    • Author(s)
      青木玲二, 森田綾子, 伏信進矢, 佐々木康幸, 小笠原由美子, 高谷直樹, 若木高善, 池田治生, 祥雲弘文
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Structure of cytochrome P450nor in complex with an NADH2003

    • Author(s)
      祥雲 弘文
    • Organizer
      12th Japanese-German Workshop on Enzyme Technology
    • Place of Presentation
      滋賀
    • Year and Date
      2003-11-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ミトコンドリア嫌気呼吸とP4502003

    • Author(s)
      祥雲 弘文
    • Organizer
      酵素工学研究会第50回講演会
    • Place of Presentation
      東京
    • Year and Date
      2003-10-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Direct electron transfer from NADH to cytochrome P450nor2003

    • Author(s)
      祥雲 弘文
    • Organizer
      The 9th Southeast Asian-Western Pacific Regional Meeting of Pharmacologists
    • Place of Presentation
      韓国
    • Year and Date
      2003-08-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 放線菌の新規窒素代謝の解析2003

    • Author(s)
      森田綾子, 青木玲二, 佐々木康幸, 高谷直樹, 伏信進矢, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2003年度大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cylindrocarpon tonkinenseによる硝酸塩の脱窒機構の解析2003

    • Author(s)
      和辻智郎, 高谷直樹, 中村顕, 祥雲弘文
    • Organizer
      日本農芸化学会2003年度大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 糸状薗Fusarium oxysporumのミトコンドリア硝酸呼吸の解析2003

    • Author(s)
      桑崎誠剛, 高谷直樹, 中村顕, 祥雲弘文
    • Organizer
      日本農芸化学会2003年度大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] P450norの共脱窒活性2003

    • Author(s)
      蘇霏, 高谷直樹, 祥雲弘文
    • Organizer
      日本農芸化学会2003年度大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] シトクロムP450 norのS73/75G変異体とNADアナログの複合窒素体の構造解析2003

    • Author(s)
      大島理絵子, 伏信進矢, 蘇霏, 張力, 高谷直樹, 若木高善, 祥雲弘文
    • Organizer
      日本農芸化学会2003年度大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Application of p450nor and P450foxy to Green Chemistry2002

    • Author(s)
      祥雲 弘文
    • Organizer
      Korea-Japan/Japan-Korea Biotechnology Industry Investment & Cooperation Conference
    • Place of Presentation
      韓国
    • Year and Date
      2002-09-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diversified functions and application of cytochrome P4502002

    • Author(s)
      祥雲 弘文
    • Organizer
      10th Anniversary Novozymes Japan Enzyme Symposium
    • Place of Presentation
      京都
    • Year and Date
      2002-09-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] THE SMALLEST BIOMOLECULES : Diatomics and their Interactions with Heme Proteins2008

    • Author(s)
      Andreas Daiber, Hirofumi Shoun, Volker Ullrich
    • Total Pages
      614
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Methods Enzymol. : Globins and Other Nitric Oxide-Reactive Proteins2008

    • Author(s)
      L. Zhang, H. Shoun
    • Total Pages
      655
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 応用微生物学 第2版2006

    • Author(s)
      清水 昌, 堀之内末治 編
    • Total Pages
      473
    • Publisher
      文永営堂出版
    • Related Report
      2005 Annual Research Report
  • [Publications] S.Kuwazaki, N.Takaya, A.Nakamura, H.Shoun: "Formate-forming fungal catabolic pathway to supply electrons to nitrate respiration"Biosci.Biotech.Biochem.. Vol.67 No.4. 937-939 (2003)

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  • [Publications] T.Watsuji, N.Takaya, A.Nakamura, H.Shoun: "Denitrification of nitrate by the fungus Cylindrocarpon tonkinense"Biosci.Biotech.Biochem.. Vol.67 No.5. 1115-1120 (2003)

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  • [Publications] T.Watsuji, N.Takaya, A.Nakamura, H.Shoun: "A possible role of NADPH-dependent cytochrome P450nor isozyme in glycolysis under denitrifying conditions"Biosci.Biotech.Biochem.. Vol.67 No.5. 1109-1114 (2003)

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  • [Publications] N.Takaya, S.Kuwazaki, Y.Adachi, S.Suzuki, T.Kikuchi, H.Nakamura, Y.Shiro, H.Shoun: "Hybrid respiration in the denitrifying mitochondria of Fusarium oxysporum"J.Biochem. Vol.133 No.4. 461-465 (2003)

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      2003 Annual Research Report
  • [Publications] N.Takaya, M.A.B.Catalan-Sakairi, Y.Sakaguchi, I.Kato, Z.Zhou, H.Shoun: "Aerobic denitrifying bacteria that produce low levels of nitrous oxide"Appl.Environ.Microbiol.. Vol.69 No.6. 461-465 (2003)

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      2003 Annual Research Report
  • [Publications] F.Su, N.Takaya, H.Shoun: "Nitrous oxide-forming codenitrification catalyzed by cytochrome P450nor"Biosci.Biotech.Biochem.. Vol.68 No.2. 473-475 (2004)

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      2003 Annual Research Report
  • [Publications] Naoki Takaya, Hirofumi Shoun: "Genetic Engineering Using Fungal Flavohemoglobin for Constructing Pseudomonas stutzen Strain Emitting Less Nitrous Oxide"Joumal of Bioscience and Bioengineering. Vol.94 No.3. 282-284 (2002)

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  • [Publications] Hiromasa Uchimura, Hitoshi Enjoji, Takafumi Seki, Ayako Taguchi, Naoki Takaya, Hirofumi Shoun: "Nitrate Reductase-Formate Dehydrogenase Couple lnvolved in the Fungal Denitrification by Fusanum oxysporum"Journal of Biochemistry. Vol.131 No.4. 579-586 (2002)

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  • [Publications] Naoki Takaya, Hiromasa Utimura, Yupeng Lai, Hirofumi Shoun: "transcriptional Control of Nitric Oxide Reductase Gene(CYP55)in the Fungal Denitrifier Fusanum oxysporum"Bioscience, Biotechnology and Biochemistry. Vol.66 No.5. 1039-1045 (2002)

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      2002 Annual Research Report
  • [Publications] Li Zhang, Takashi Kudo, Naoki Takaya, Hirofumi Shoun: "The B' Helix Determins Cytochrome P450nor Specificity for the Electron Donors NADH and NADPH"The Journal of Biological Chemistry. Vol.277 No.37. 33842-33847 (2002)

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      2002 Annual Research Report
  • [Publications] Yasuyuki Kumon, Yasuyuki Sasaki, Isao Kato, Naoki Tkaya, Hirofumi Shoun, Teruhiko Beppu: "Codenitrification and Denitrification Are Dual Metabolic Pathways through Which Dinitrigen Evolves from Nitrate in Streptomyces antibioticus"Journal of Bacteriology. Vol.184 No.11. 2963-2968 (2002)

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Published: 2002-04-01   Modified: 2016-04-21  

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