| Project/Area Number |
14104007
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Kinki University |
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Principal Investigator |
KUMAI Hidemi Kinki University, Fisheries Laboratory, Professor, 水産研究所, 教授 (60088656)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Osamu Kinki University, Fisheries Laboratory, Professor, 水産研究所, 教授 (70088657)
MIYASHITA Shigeru Kinki University, Fisheries Laboratory, Professor, 水産研究所, 教授 (80088658)
TAKII Kenji Kinki University, Fisheries Laboratory, Professor, 水産研究所, 教授 (60197225)
OHTA Hiromi Kinki University, Department of Fisheries, Professor, 農学部, 教授 (10351579)
TANAKA Masaru Kyoto University, FSERC, Professor, フィールド研, 教授 (20155170)
神原 淳 三重大学, 生物資源学部, 教授 (90183334)
細川 秀毅 高知大学, 農学部, 教授 (40036744)
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| Project Period (FY) |
2002 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥115,050,000 (Direct Cost: ¥88,500,000、Indirect Cost: ¥26,550,000)
Fiscal Year 2006: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2005: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2004: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2003: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2002: ¥52,260,000 (Direct Cost: ¥40,200,000、Indirect Cost: ¥12,060,000)
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| Keywords | Bluefin tuna / Resource propagation / Maturation and spawning / Larviculture / Seedling production / Nutrient metabolism / Feed development / Full-cycle culture / 初期発育 |
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| Research Abstract |
Biological and biochemical studies on the reproductive biology, larval and juvenile development, and nutritional requirements were conducted in order to promote resource propagation of Pacific bluefin tuna (PBT) Thunnus orientalis. In three research sections, maturation and spawning, early development and larviculture, and nutrition and feed development, valuable knowledge was accumulated.
1. The maturation and spawning section demonstrated that (1) the maturation of PBT were promoted by the elongation of day-length and the elevation of water temperature ; (2) active spawning occurred at a water temperature of 27℃ and day-length of 14 hours ; (3) aromatase gene expressed in the ovary could be used as an indicator for estimation of the female maturation ; and (4) the storage of sperm in liquid nitrogen was useful for the artificial insemination of PBT.
2. The early development and larviculture section characterized (1) the fast growth and unique metamorphosis of larvae and juveniles with the changes in DNA-RNA contents, thyroid hormone levels and digestive enzyme activities ; and (2) suitable rearing protocol such as water temperature, salinity and light intensity for larviculture.
3. The nutrition and feed development section identified (1) the feeding stimulants ; and (2) optimal dietary level of protein, lipids and vitamin C ; and was able to develop (3) artificial diet for juveniles. Based on these findings, we succeeded in managing the complete PBT life cycle under aquaculture conditions in 2002 and have successfully brought full-cycle cultured PBT to market starting in 2004. Finally, we could produce and transfer more than 10,000 juveniles from land tanks to sea net cage in 2006.
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