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Comprehensive analysis of bone formation and its application

Research Project

Project/Area Number 14104015
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionTokyo Medical and Dental University (2004-2006)
Nagasaki University (2002-2003)

Principal Investigator

YAMAGUCHI Akira  Tokyo Medical and Dental University, Graduate School, Oral Pathology, Professor, 大学院医歯学総合研究科, 教授 (00142430)

Co-Investigator(Kenkyū-buntansha) KATSUBE Ken-ichi  Tokyo Medical and Dental University, Graduate School, Oral Pathology, Assistant Professor, 大学院医歯学総合研究科, 講師 (20233760)
SAKAMOTO Kei  Tokyo Medical and Dental University, Graduate School, Oral Pathology, Research Associate, 大学院医歯学総合研究科, 助手 (00302886)
TAMAMURA Sadahiro  Tokyo Medical and Dental University, Graduate School, Oral Pathology, Research Associate, 大学院医歯学総合研究科, 特任助手 (70431963)
柴田 恭明  長崎大学, 大学院医歯薬学総合研究科, 助手 (80253673)
塚崎 智雄  長崎大学, 大学院・医歯薬学総合研究科, 助教授 (50315230)
藤田 修一  長崎大学, 大学院・医歯薬学総合研究科, 助手 (00181355)
進藤 裕幸  長崎大学, 大学院・医歯薬学総合研究科, 教授 (30107677)
原 宜興  長崎大学, 大学院・医歯薬学総合研究科, 教授 (60159100)
Project Period (FY) 2002 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥113,230,000 (Direct Cost: ¥87,100,000、Indirect Cost: ¥26,130,000)
Fiscal Year 2006: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2005: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2004: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2003: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2002: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Keywordsosteoblast / Runx2 / CCN3 / Notch / Mesenchymal stem cell / frog / evolution / bone regeneration / 骨形成 / BMP / アフリカツメガエル / 軟骨内骨化 / 再生 / 再生療法 / 細胞分化 / 両生類
Research Abstract

We investigate the following issues in this research. 1)Analyses of regulatory factors of osteoblast differentiation, 2)Establishment and characterization of mesenchymal stem cells, 3)Evolutional changes of skeletal tissues. Results in this study is as follow.
1)Analyses of regulatory factors of osteoblast differentiation
a)We established runx2-deficient cell lines, and demonstrate that osteoblast differentiation is induced by BMP-2 in these cells by using other transcription factors such as Dlx5 and Msx2. b)CCN3 (NOV) inhibits osteoblast differentiation by interacting with BMP and Notch signaling pathways. c)We revealed the important role of Msx2 in osteoblast differentiation.
2)Establishment and characterization of mesenchymal stem cells
a)We established mesenchymal stem cells from human hamartoma, and characterized their differentiation potencies into various mesenchymal cells, b)We established multipotent mesenchymal cell lines form human osteosarcoma cell lines, which exhibited differ … More entiation potential into osteoblasts, chondrocytes, and adipocytes, c)We investigated the differentiation potential of multipotent KUSA cells, which were isolated from murine bone marrow, and revealed that CCN3 inhibits osteoblast differentiation in this cell line.
3)Evolutional changes of skeletal tissues
a)We demonstrated that endochondral ossification is delayed prominently in frog long bones, compared with that in mammals. This was caused by the different expression profiles of Banded Hedgehog in frog, compared with expression profiles of Indian Hedgehog in mouse long bones. b)We established cell culture methods for muscle and chondrocytes isolated frogs. c)We compared the bone regeneration process between frog and mouse. Bone regeneration process was extremely delayed, compared with that of mouse. d)We investigated whether bones in frogs play important role in calcium reservoir, and suggested that the bones in frogs will not be involved in calcium reservoir.
These results will provide important information to establish new therapies for bone regeneration and osteoporosis. Less

Report

(6 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (54 results)

All 2007 2006 2005 2004 2003 Other

All Journal Article (42 results) Publications (12 results)

  • [Journal Article] BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.2007

    • Author(s)
      Liu T, Gao Y, Sakamoto K, Minamizato T, Furukawa K, Tsukazaki T, Shibata Y, Bessho K, Komori T, Yamaguchi A
    • Journal Title

      J Cell. Physiol 211

      Pages: 728-735

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CCN3/NOV inhibits BMP-2-induced osteoblast differentiation by interacting with BMP and Notch signaling pathways.2007

    • Author(s)
      Minamizato T, Sakamoto K, Liu T, KokubO H, Katsube K, Perbal B, Nakamura S, Yamaguchi A
    • Journal Title

      Biochem Biophys Res Commun 354

      Pages: 567-573

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of osteoclast differentiation and function by the CaMK/CREB pathway.2007

    • Author(s)
      Sato K, Suematsu A, Nakashima T, Takemoto- Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, Chatila TA, Bito H, Takayanagi H
    • Journal Title

      Nat Med 12

      Pages: 1410-1416

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Th17 functions as an osteoclastogenic helper T cell subset that links T cell activation and bone destruction.2007

    • Author(s)
      Sato K, Okamoto K, Suematsu A, Yamaguchi A, Morishita Y, Kadono Y, Tanaka S, Kodama T, Akira S, Iwakura Y, Cua DJ, Takayanagi H
    • Journal Title

      J Exp Med 203

      Pages: 2673-2682

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.2007

    • Author(s)
      Liu T, Gao Y, Sakamoto K, Minamizato T, Furukawa K, Tsukazaki T, Shibata Y, Bessho K, Komori T, Yamaguchi A
    • Journal Title

      J Cell.Physiol 211

      Pages: 728-735

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CCN3/NOV inhibits BMP-2-induced osteoblast differentiation by interacting with BMP and Notch signaling pathways.2007

    • Author(s)
      Minamizato T, Sakamoto K, Liu T, Kokubo H, Kats be K, Per bal B, Nakamura S, Yamaguchi A
    • Journal Title

      Biochem Biophys Res Commun 354

      Pages: 567-573

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of osteoclast differentiation and function by the CaMK/CREB pathway.2007

    • Author(s)
      Sato K, Suematsu A, Nakashima T, Takemoto-Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, Chatila TA, Bito H, Takayanagi H
    • Journal Title

      Nat Med 12

      Pages: 1410-1416

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modulation of mouse RANKL gene expression by Runx2 and PKA pathway.2006

    • Author(s)
      Mori K, Kitazawa R, Kondo T, Maeda S, Yamaguchi A, Kitazawa S
    • Journal Title

      J Cell Biochem 98

      Pages: 1629-1644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modulation of mouse RANKL gene expression by Runx2 and PKA pathway.2006

    • Author(s)
      Mori K, Kitazawa T, Kondo T, Maeda S, Yamaguchi A, Kitazawa S
    • Journal Title

      J Cell Biochem. 98

      Pages: 1629-1644

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An experimental model of tooth movement by orthodontic force in mice and its application to TNF receptor-deficient mice.2006

    • Author(s)
      Yoshimatsu M, Shibata Y, Yoshida N, Yamaguchi A, et al.
    • Journal Title

      J Bone Miner Metab 24

      Pages: 20-27

    • Related Report
      2006 Annual Research Report
  • [Journal Article] TNF receptor loop peptide mimic blocks RANK ligand-induced signaling, bone resorption and bone loss"(22513-RG-RV-2).2006

    • Author(s)
      Aoki K, Saito H, Yamaguchi A, Deprez P, Murali R, Ohya K, Horne W, Baron R, et al.
    • Journal Title

      J Clin Invest 116

      Pages: 1525-1534

    • Related Report
      2006 Annual Research Report
  • [Journal Article] CSF1 gene associated with aggressive periodontitis in the Japanese population2006

    • Author(s)
      Rabello D, Yamaguchi A, Kojima T, et al.
    • Journal Title

      Biochem Biophys Res Commun 347

      Pages: 791-796

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of RANKL/RANK/OPG gene polymorphism in aggressive periodontitis.2006

    • Author(s)
      Soedarsono N, Yamaguchi A, Kojima T, et al.
    • Journal Title

      J Period Res 41

      Pages: 791-796

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Modulation of mouse RANKL gene expression by Runx2 and PKA pathway.2006

    • Author(s)
      Mori K, Kitazawa R, Kondo T, Maeda S, Yamaguchi A, Kitazawa S, et al.
    • Journal Title

      J Cell Biochem 41

      Pages: 1629-1644

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Th17 functions as an osteoclastogenic helper T cell subset that links T cell activation and bone destruction.2006

    • Author(s)
      Sato K, Okamoto K, Yamaguchi A, Takayanagi H, et al.
    • Journal Title

      J Exp Med 203

      Pages: 2673-2682

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An experimental model of tooth movement by orthodontic force in mice and its application to TNF receptor-deficient mice.2006

    • Author(s)
      Yoshimatsu M, Shibata Y, Kitaura H, Chang X, Moriishi T, Hashimoto F, Yoshida N, Yamaguchi A
    • Journal Title

      J Bone Miner Metab. 24

      Pages: 20-27

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evaluation of RANKL/RANK/OPG gene polymorphism in aggressive periodontitis.2006

    • Author(s)
      Nurtami S, Robello D, Ishihara U, Noguchi T, Yamaguchi A, Kojima T
    • Journal Title

      J Pepriod Res In press

    • Related Report
      2005 Annual Research Report
  • [Journal Article] CCAAT/enhancer binding protein β isoform, LIP, regulates commitment of osteoblasts and adipocytes.2005

    • Author(s)
      Hata K, Nishimura R, Ueda M, Ikeda F, Matsubara T, Ichida F, Hisada K, Nokubi T, Yamaguchi A, Yoneda T
    • Journal Title

      Mol Cell Biol. 25

      Pages: 1971-1979

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Expression profile of Xenopus banded hedgehog, a homolog of mouse Indian hedgehog, is related to the late development of endochondral ossification in Xenopus laevis.2005

    • Author(s)
      Moriishi T, Shibata Y, Tsukazaki T, Yamaguchi A
    • Journal Title

      Biochem Biophys Res Commun 328

      Pages: 867-873

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distinct roles of EGF repeats for the Notch signaling system.2005

    • Author(s)
      Sakamoto K, Wang SC, Katsube K, Yamaguchi A
    • Journal Title

      Exp Cell Res 302

      Pages: 281-291

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by Deltal/Jaggedl-activated Notchl signaling.2005

    • Author(s)
      Nobta M, Tsukazaki T, Shibata Y, Chang X, Moriishi T, Sakano S, Shindo H, Yamaguchi A
    • Journal Title

      J Biol Chem 280

      Pages: 15842-15848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.2005

    • Author(s)
      Nobuta M, Tsukazaki T, Shibata Y, Chang X, Moriishi T, Sakano S, Shindo H, Yamaguchi A
    • Journal Title

      J Biol Chem 280

      Pages: 15842-15848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distinct roles of EGF repeats for the Notch signaling system2005

    • Author(s)
      Sakamoto K, Wang SC, Katsube K, Yamaguchi A
    • Journal Title

      Exp Cell Res 302

      Pages: 281-291

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression profile of Xenopus banded hedgehog, a homolog of mouse Indian hedgehog, is related to the late development of endochondral ossification in Xenopus laevis2005

    • Author(s)
      Moriishi T, Shibata Y, Tsukazaki T, Yamaguchi A
    • Journal Title

      Biochem Biophys Res Commun 328

      Pages: 867-873

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling..2005

    • Author(s)
      Nobta M, Tsukazaki T, Shibata Y, Chang X, Moriishi T, Sakano S, Shindo H, Yamaguchi A
    • Journal Title

      J.Biol.Chem 280

      Pages: 15842-15848

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Abnormal expression of Rb pathway-related proteins in salivary gland acinic cell carcinoma.2005

    • Author(s)
      Liu T, Zhu E, Wang L, Okada T, Yamaguchi A, Okada N
    • Journal Title

      Humam Pathology 36

      Pages: 962-970

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Frequent promoter hypermethylation of RASSF1A and p16^<IN^<K4a>> and infrequent allelic loss other than 9p21 in Betel-associated oral carcinoma in a Vietnamese non-smoking/non-drinking female population.2005

    • Author(s)
      Tran Tu N, Liu Y, Takagi M, Yamaguchi A, Fujii H
    • Journal Title

      J.Oral Pathol.Med. 34

      Pages: 150-156

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Human mucoepidermoid carcinomas expressed Hairy-Enhancer of Split homologue 1 (HES1), a downstream transcriptional factor of Notch signal.2005

    • Author(s)
      Ono F, Mikami S, Sudo T, Okada N, Yamaguchi A, Katsube K
    • Journal Title

      J.Oral Pathol.Med. 10

      Pages: 107-113

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of p53, mdm2 and p21 proteins in betal quid and tobacco associated oral squamous cell carcinoma in Bangladeshi population.2005

    • Author(s)
      Hossain MMI, Uzzama MA, Nishioka M, Sakamoto K, Renelson E, Yamaguchi A, Takagi M
    • Journal Title

      J.Oral Pathol.Med. 10

      Pages: 23-31

    • NAID

      130004850515

    • Related Report
      2005 Annual Research Report
  • [Journal Article] CCAAT/enhancer binding protein β isoform, LIP, regulates ommitment of osteoblasts and adipocytes.2005

    • Author(s)
      Hata K, Yamaguchi A, et al.
    • Journal Title

      Mol Cell Biol. 25

      Pages: 1971-1979

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.2005

    • Author(s)
      Nobuta M, Tsukazaki T, Yamaguchi A, et al.
    • Journal Title

      J.Biol.Chem. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma.2004

    • Author(s)
      Doiguchi Y, Tsukazaki T, Tomonag T, Nobuta M, Fujita S, Hayashi T, Nagai K, Matsumoto T, Shindo H, Yamaguchi A
    • Journal Title

      Cell Tissue Res 317

      Pages: 237-246

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reciprocal roles of Msx2 in regulation of osteoblast and adipocyte differentiation.2004

    • Author(s)
      Ichida F, Nishimura R, Hata K, Matsubara T, Ikeda F, Hisada K, Yatani H, Cao X, Komori T, Yamaguchi A, Yoneda T
    • Journal Title

      J Biol Chem 279

      Pages: 34015-34022

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a mesenchymal cell line that differentiates into osteoblasts in response to BMP-2 from calvariae of GFP transgenic mice.2004

    • Author(s)
      Kadowaki A, Tsukazaki T, Hirata K, Shibata Y, Okubo Y, Bessho K, Komori T, Yoshida N, Yamaguchi A
    • Journal Title

      BONE 34

      Pages: 993-1003

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of a new member of IGFBP superfamily, IGFBP-rP10, in proliferation and differentiation of osteoblastic cells.2004

    • Author(s)
      Shibata Y, Tsukazaki T, Hirata K, Cheng X, Yamaguchi A
    • Journal Title

      Biochem Biophys Res Commun 325

      Pages: 1194-1200

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma.2004

    • Author(s)
      Doiguchi Y, Tsukazaki T, Tomonaga T, Nobuta M, Fujita S, Hayashi T, Nagai K, Matsumoto T, Shindo H, Yamaguchi A
    • Journal Title

      Cell Tissue Res 317

      Pages: 237-246

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a mesenchymal cell line that differentiates into osteoblasts in response to BMP-2 from calvariae GFP transgenic mice.2004

    • Author(s)
      Kadowaki A, Tsukazaki T, Yoshida N, Yamaguchi A
    • Journal Title

      BONE 34

      Pages: 993-1003

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reciprocal roles of Msx2 in regulation of osteoblast and adipocyte differentiation2004

    • Author(s)
      Ichida F, Nishimura R, Yamaguchi A, Yoneda T., et al.
    • Journal Title

      J Biol Chem 279

      Pages: 34015-34022

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation and characterization of a mesenchymal cell line that differentiates into osteoblasts in response to BMP-2 from calvariae of GFP transgenic mice.2004

    • Author(s)
      Kadowaki A, Yamaguchi A, et al.
    • Journal Title

      BONE 34

      Pages: 993-1003

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Establishment of a clonal human mesenchymal cell line that retains multilineage diifferentiation capacity from a spinal hamartoma.2004

    • Author(s)
      Doiguchi Y, Yamaguchi A, et al.
    • Journal Title

      Cell Tissue Res 317

      Pages: 237-246

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of a new member of IGFBP superfamily, IGFBP-rP10, in proliferation and differentiation of osteoblastic cells.2004

    • Author(s)
      Shibata Y, Yamaguchi A, et al.
    • Journal Title

      Biochem Biophys Res Commun 325

      Pages: 1194-1200

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2.2003

    • Author(s)
      Hirata K, Tsukazaki T, Kadowaki A, Furukawa K, Shibata Y, Moriishi T, Okubo Y, Bessho K, Komori T, Mizuno A, Yamaguchi A
    • Journal Title

      Bone 32

      Pages: 502-512

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Publications] Ochi K, Chen G, Ushida T, Gojo S, segawa K, Tai H, Ueno K, Ohkawa H, Mori T, Yamaguchi A, Toyama Y, Hata J, Umezawa A: "Use of mature osteoblasts in abundance act as desired -shaped bone regeneration in combination with a modified poly DL-Lactic-co-glycolic acid(PLGA)-collagen sponge"J Cell.Physiol. 194. 45-53 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shirota T, Tashiro M, Ohno K, Yamaguchi A: "Effect of intermittent parathyroid hormone (1-34) treatment on the bone response after placement of titanium implants into the tibia of ovariectomized rats"J Oral Maxillofac Surg. 61. 471-480 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirata K, Tsukazaki T, Kadowaki A, Furukawa K, Shibata Y, Moriishi T, Okubo Y, Bessho K, Komori T, Mizuno A, Yamaguchi A: "Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2"BONE. 32. 502-512 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iioka T, Furukawa K, Yamaguchi A, Shindo H, Yamashirta S, Tsukazaki T: "p300/CBP acts as a coactivator to cartilage homeoprotein-1 (Cart1), paired-like homeoprotein, through acetylation of the con served lysine residue adjacent to the homeodomain"J.Bone Miner.Res. 18. 1419-1429 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shindo K, Kawashima N, Sakamoto K, Yamaguchi A, Umezawa A, Takagi M, Katsube K, Suda H.: "Osteogenic differentiation of the mesenchymal progenitor cells, Kusa is suppressed by Notch signaling"Exp Cell Res.. 290. 370-380 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Kadowaki, T.Tsukazaki, K.Hirata, Y.Shibata, Y.Okubo, K.Bessho, T.Komori, N.Yoshida, A.Yamaguchi: "Isolation and characterization of a mesenchymal cell line that differentiates into osteoblasts in response to BMP-2 from calvariae of GFP transgenic mice"BONE. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirata K, Tsukazaki T, Yamaguchi A, et al.: "Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2"BONE. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ochi K, Chen G, Yamaguchi A, Umezawa A, et al.: "Use of mature osteoblasts in abundance act as desired -shaped bone regeneration in combination with a modified poly DL-Lactic-co-glycolic acid (PLGA)-collagen sponge"J. Cell. Physiol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuasa T, Kataoka H, Kinto N, Shibata Y, Yamaguchi A, et al.: "Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2"J. Cell. Physiol.. 193. 225-232 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Furukawa K, Yamaguchi A, Tsukazaki T, et al.: "Functional domains of paired-like homeoprotein Cart1 and the relationship between dimerization and transcription activity"Genes Cells. 11. 1135-1147 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shibata Y, Yamaguchi A, Koji T, et al.: "Inhibition apoptosis and possible involvement of Fas/Fas ligand to avoid apoptosis of minor salivary gland in Sjogren's syndrome"Hum. Cell. 15. 52-60 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Merida LM, Yamaguchi A, Kawai S, et al.: "Effects of vitamin D analog, 22-oxa-1,25 dihydroxyvitamin D3, on bone reconstruction by vascularized bone allograft"BONE. 30. 422-427 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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