• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The genome dynamics and function in higher plants

Research Project

Project/Area Number 14204075
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionNational Institute for Basic Biology (2004)
Okazaki National Research Institutes (2002-2003)

Principal Investigator

IIDA Shigeru  National Institute for Basic Biology, Professor, 分子遺伝学研究部門, 教授 (30012777)

Co-Investigator(Kenkyū-buntansha) TERADA Rie  National Institute for Basic Biology Division of Molecular Genetics, Research associate, 分子遺伝学研究部門, 助手 (30137799)
HOSHINO Atsushi  National Institute for Basic Biology Division of Molecular Genetics, Research associate, 分子遺伝学研究部門, 助手 (80312205)
TSUGANE Kazuo  National Institute for Basic Biology Division of Molecular Genetics, Research associate, 分子遺伝学研究部門, 助手 (50343744)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2004: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2003: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2002: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Keywordsmorning-glories / rice / genome / spontaneous mutation / transposon / mutable allele / gene targeting / gene tagging / 遺伝子ターゲティン / マルバアサガオ / ソライロアサガオ
Research Abstract

The spontaneous magenta, pink and fuchsia mutants in I. nil, I. purpurea and I. tricolor exhibit reddish flowers, respectively, while their wild-type flowers display blue or dark purple flowers. We have identified that these mutants carry either point mutations or transposon insertions within the F3'H gene encoding flavonoid 3'-hydroxylase that mediates hydroxylation of the 3' position of the B-ring of naringenin and dehydrokaempferol in anthocyanin pigment biosynthesis. The reddish-purple buds of the wild-type I. nil change into blue open flowers, and the shift in the flower coloration correlates with an increase in the vacuolar pH of the flower epidermal cell. In the mutant deficient in the NHX1 gene for the vacuolar Na+/H+ antiporter, the vacuolar alkalization occurs only partially, and reddish-purple buds become purple open flowers. We have identified a novel vacuolar Na+/H+ antiporter gene NHX2, which is one of the genes responsible for converting reddish-purple buds into purple open flowers by partially increasing vacuolar pH in the absence of the NHX1 activity. The dusky mutants of I. nil displaying reddish-brown or purplish-gray flowers were found to be deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose : anthocyanidin 3-O-glucoside-2"-O-glucosyltransferase (3GGT), which mediates the glucosylation of anthocyanidin 3-O-glucosides to yield 3-O-sophorosides. All of the dusky mutants examined were shown to carry 4 by insertions at the identical position in the 3GGT gene, and the insertions caused frameshift mutations.
Modification of genes through homologousrecombination termed gene targeting is the most direct method to characterize gene function, and we have succeeded in modifying the rice genomic sequence of the Waxy gene reproducibly by using a strong positive-negative selection. We also identified a novel rice DNA transposon, nDart, which can be used for gene tagging in rice.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (33 results)

All 2005 2004 2003 2002 Other

All Journal Article (18 results) Book (2 results) Publications (13 results)

  • [Journal Article] Characterization of a novel Na^+/H^+ antiporter gene InNHX2 and comparison of InNHX2 with InNHX1, which is responsible for blue flower coloration by increasing the vacuolar pH in the Japanese morning glory.2005

    • Author(s)
      M.Ohnishi
    • Journal Title

      Plant Cell Physiology 46

      Pages: 259-267

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Japanese morning glory dusky mutants displaying reddish-brown or purplish-grey flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose : anthocyanidin 3-O-glucoside 2"-O-glucosyltransferase due to 4-bp insertions in the gene.2005

    • Author(s)
      Y.Morita
    • Journal Title

      The Plant Journal 42

      Pages: 353-363

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of a novel Na+/H+ antiporter gene InNHX2 and comparison of InNHX2 with InNHX1, which is responsible for blue flower coloration by increasing the vacuolar pH in the Japanese morning glory.2005

    • Author(s)
      M.Ohnishi
    • Journal Title

      Plant Cell Physiology 46

      Pages: 259-267

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Japanese morning glory dusky mutants displaying reddish-brown or purplish-grey flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose : anthocyanidin 3-O-glucoside-2"-O- Glucosyltransferase due to 4-bp insertions in the gene.2005

    • Author(s)
      Y.Morita
    • Journal Title

      The Plant Journal 42

      Pages: 353-363

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of a novel Na+/H+ antiporter gene InNHX2 and comparison of InNHX2 with InNHX1, which is responsible for blue flower coloration by increasing the Vacuolar pH in the Japanese Morning Glory2005

    • Author(s)
      M.Ohnishi
    • Journal Title

      Plant and cell Physiology 46

      Pages: 259-267

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Japanese morning glory dusky mutants displaying reddish-brown or purplish-grey flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertions in the gene.2005

    • Author(s)
      Y.Morita
    • Journal Title

      The Plant Journal (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A tale of two integrations, transgene and T-DNA : gene targeting by homologous recombinetion in rice.2004

    • Author(s)
      S.Iida
    • Journal Title

      Current Opition in Biotechnology 15

      Pages: 132-138

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An intragenic tandem duplication in a transcriptional regulatory gene for anthocyanin biosynthesis confers pale-colored flowers and seeds with fine spods in Ipomoea tricolor.2004

    • Author(s)
      K.I.Park
    • Journal Title

      The Plant Journal 38

      Pages: 840-849

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A tale of two integrations, transgene and T-DNA : gene targeting by homologous recombination in rice.2004

    • Author(s)
      S.Iida
    • Journal Title

      Current Opinion in Biotechnology 15

      Pages: 132-138

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An intragenic tandem duplication in a transcriptional regulatory gene for anthocyanin biosynthesis confers pale-colored flowers and seeds with fine spots in Ipomoea tricolor.2004

    • Author(s)
      K.I.Park
    • Journal Title

      The Plant Journal 38

      Pages: 840-849

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetics and epigenetics in flower pigmentation associated with transposable elements in morning glories.2004

    • Author(s)
      S.Iida
    • Journal Title

      Advanced in Biophysics 38

      Pages: 141-159

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection for gene targeting in rice(Oryza sativa L).2004

    • Author(s)
      R.Terada
    • Journal Title

      Plant Cell Reports 22

      Pages: 653-659

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic and epigenetics in flower pigmentation associated with transposable elements in morning glories.2004

    • Author(s)
      S.Iida
    • Journal Title

      Advanced in Biophysics 38

      Pages: 141-159

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A tale of two integration, transgene and T-DNA : gene targeting by homologous recombination in rice.2004

    • Author(s)
      S.Iida
    • Journal Title

      Current Opinion in Biotechnology 15

      Pages: 132-138

    • Related Report
      2004 Annual Research Report
  • [Journal Article] An intragenic tandem duplication in a transcriptionai regulatory gene for anthocyanin biosynthesis confers pale-colored flowers and seeds with fine spots iin Ipomoe2004

    • Author(s)
      K.I.Park
    • Journal Title

      The Plant Journal

      Pages: 840-849

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Spontaneous mutations of the flavonoid 3' -hydroxylase gene conferring reddish flowers in the three morning glory species.2003

    • Author(s)
      A.Hoshino
    • Journal Title

      Plant Cell Physiology 44

      Pages: 990-1001

    • NAID

      10011704125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spontaneous mutations of the flavonoid 3'-hydroxylase gene conferring reddish flowers in the three morning glory species.2003

    • Author(s)
      A.Hoshino
    • Journal Title

      Plant Cell Physiology 44

      Pages: 990-1001

    • NAID

      10011704125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Efficient gene targeting by homologous recombination in rice.2002

    • Author(s)
      R.Terada
    • Journal Title

      Nature Biotechnology 20

      Pages: 1030-1034

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] エピジェネティックス2005

    • Author(s)
      星野 敦
    • Total Pages
      238
    • Publisher
      シュプリンガー・フェアラーク
    • Related Report
      2004 Annual Research Report
  • [Book] Advanced in Biophysics Vol.38,pp.141-1592004

    • Author(s)
      S.Iida
    • Total Pages
      235
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] T.Kikuchi: "An efficient conversion of catechine into 3,4-trans-leucocyanidin."Heterocycles. 60. 1469-1475 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Hoshino: "Spontaneous mutations of the flavonoid 3'-hydroxylase gene conferring reddish flowers in the three morning glory species."Plant and cell Physiology. 44. 990-1001 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] R.Terada: "A large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection for gene targeting in rice (Oryza sativa L.)."Plant Cell Reports. 22(in press). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Iida: "Genetic and epigenetics in flower pigmentation associated with transposable elements in morning glories."Advanced in Biophysics. 38(in press). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Iida: "A tale of two integration, transgene and T-DNA : gene targeting by homologous recombination in rice."Current Opinion in Biotechnology. 15(in press). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.I.Park: "An intragenic tandem duplication in a transcriptional regulatory gene for anthocyanin biosynthesis confers pale-colored flowers and seeds with fine spots in Ipomoea tricolor."The Plant Journal. (in press). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 星野 敦: "エピジェネティックス"シュプリンガー・フェアラーク(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] R.Terada: "Efficient gene targeting by homologous recombination in rice"Nature Biotechnology. 20. 1030-1034 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 石川直子: "アサガオ : 園芸植物からモデル植物へ"遺伝(別冊、遺伝学はゲノム情報でどう変わるか). 15. 32-41 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 星野 敦: "アサガオ花弁の液胞pH上昇と青色化"細胞工学別冊, 植物細胞工学シリーズ. 17. 85-87 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 星野 敦: "転写調節遺伝子と液胞内pHによる花色の制御"蛋白質・核酸・酵素 (増刊号 : 植物の形づくり). 47. 1745-11752 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 飯田 滋: "特集にあたって"蛋白質・核酸・酵素. 47. 197-199 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 星野 敦: "花を彩る遺伝子"蛋白質・核酸・酵素. 47. 210-216 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi