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Studies on structure and function of organelles dividing machines based on genome information

Research Project

Project/Area Number 14204078
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionRikkyo University (2003-2005)
The University of Tokyo (2002)

Principal Investigator

KUROIWA Tsuneyoshi  Rikkyo University, College of Sciences, Professor, 理学部, 教授 (50033353)

Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥57,070,000 (Direct Cost: ¥43,900,000、Indirect Cost: ¥13,170,000)
Fiscal Year 2005: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2004: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2003: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2002: ¥43,420,000 (Direct Cost: ¥33,400,000、Indirect Cost: ¥10,020,000)
KeywordsCyanidioschyzon merolae / PD ring / plastid division / MD ring / mitochondrial division / FtsZ ring / organelle division / Cyanidioschyzon merolae(シゾ / 色素体分裂装置 / ミトコンドリア分裂装置 / ゲノム情報 / TOF-MS / シゾン / ゲノム解析 / 細胞核ゲノム / オルガネラ分裂装置 / ミトコンドリア分裂・増殖 / 葉緑体分裂・増殖 / Cyanidoschyzon merolae / ミトコンドリアの分裂 / 葉緑体の分裂
Research Abstract

To elucidate the mechanism of mitochondria and plastid division under the control of the cell nucleus, we provided the primitive red alga Cyanidioschyzon merolae as a material. C.merolae cells have very advantageous for organelle divisions because the cell contains minimum number of organelles of which divisions were synchronized by light and dark cycles. The obtained data are compared with those of higher organisms. We first examined plastid dividing (PD) ring apparatus as the circumference of PD ring was 2.5 times longer than that of mitochondrial dividing (MD) ring apparatus. To obtain pure proteins from intact PD ring apparatus, we improved several points such as efficiency of synchronization of chloroplast division, isolation of pure dividing chloroplasts, isolation of intact PD ring apparatus by various detergent treatments and complete extraction of proteins from isolated intact PD ring apparatus. We then obtained SDS gel spots by using two dimensional electrophoresis and analyzed the spots by MALDI-TOFMS on basis of C.merolae genome information. We identified about 30 genes related to PD ring fractions and made their antibodies. We found at least novel 4 genes which are contained in PD ring apparatus and continue to find more genes related intimately to organelle divisions according to the established methods.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (34 results)

All 2006 2005 2004 2003 Other

All Journal Article (20 results) Book (2 results) Publications (12 results)

  • [Journal Article] Structure, function and evolution of the mitochondrial dividing apparatus2006

    • Author(s)
      Kuroiwa, T. et al.
    • Journal Title

      Biochimica Biphysica Acta (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GENERATIVE CELL SPECIFIC l is essential for angiosperm fertilization.2006

    • Author(s)
      Mori T.et al.
    • Journal Title

      Nat.Cell Biol. 8

      Pages: 64-71

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Active digestion of sperm mitochondrial DNA in single living sperm revealed by optical tweezers.2006

    • Author(s)
      Nishimura Y. et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 103

      Pages: 1382-1387

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae.2005

    • Author(s)
      Nishida, K. et al.
    • Journal Title

      Mol. Biol. Cell. 16

      Pages: 2493-2502

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae.2005

    • Author(s)
      Nishida, K. et al.
    • Journal Title

      Mol.Biol.Cell. 16

      Pages: 2493-2502

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae.2005

    • Author(s)
      Nishida K. et al.
    • Journal Title

      Mol.Biol.Cell. 16

      Pages: 2493-2502

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cyanidioschyzon merolae genome. A Tool for Facilitating Comparable Studies on Organelle Biogenesis in Photosynthetic Eukaryotes.2005

    • Author(s)
      Misumi O. et al.
    • Journal Title

      Plant Physiol. 137

      Pages: 567-585

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phylogenetic implications of the CAD complex from the primitive red alga Cyanidioschyzon merolae (Cyanidiales, Rhodophyta).2005

    • Author(s)
      Nozaki H. et al.
    • Journal Title

      J.Phycol. 41

      Pages: 652-657

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of lysosomes-like structures in a unicellular red alga Cyanidioschyzon merolae.2005

    • Author(s)
      Yagisawa F. et al.
    • Journal Title

      Cytologia 70

      Pages: 351-354

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genome sequence of the ultra small Unicellular red alge Cyanidioscbyzon merolae lOD.2004

    • Author(s)
      Matsuzaki, M., et al.
    • Journal Title

      Nature 428

      Pages: 653-657

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genome sequence of the ultrasmall Unicellular red alga Cyanidioschyzon merolae 10D.2004

    • Author(s)
      Matsuzaki, M., et al.
    • Journal Title

      Nature 428

      Pages: 653-657

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The terminal eukaryotic ribosomal DNA units in the primitive red alga Cyanidioschyzon merolae2004

    • Author(s)
      Maruyama et al.
    • Journal Title

      DNA Res. 11

      Pages: 83-91

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Two types of FtsZ proteins in mitochondria and red-lineage chloroplasts : the duplication of FtsZ is implicated in endosynmbiosis.2004

    • Author(s)
      Miyagishima et al.
    • Journal Title

      J.Mol.Evol. 58

      Pages: 291-301

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Organelle nuclei in higher plants : structure, composition, function, and evolution.2004

    • Author(s)
      Sakai et al.
    • Journal Title

      Int.Rev.Cytol. 238

      Pages: 59-118

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Improvement of cultures conditions and evidence for nuclear tranformation by homologous recombination in a red alga, Cyanidioschyzon merolae2004

    • Author(s)
      Minoda et al.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 667-671

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae2004

    • Author(s)
      Matsuzaki et al.
    • Journal Title

      Nature 428

      Pages: 653-657

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation of cycloheximide-resistant mutants of Cyanidioschyzon merolae2004

    • Author(s)
      Yagisawa et al.
    • Journal Title

      Cytologia 69

      Pages: 97-100

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The division of pleomorphic plastids with multiple FtsZ rings in tobacco BY-2 cells.2003

    • Author(s)
      Momoyama, Y.
    • Journal Title

      Europ. J. Cell Biol. 82

      Pages: 323-332

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The division of pleomorphic plastids with multiple FtsZ rings in tobacco BY-2 cells.2003

    • Author(s)
      Momoyama, Y., et al.
    • Journal Title

      Europ.J.Cell Biol. 82

      Pages: 323-332

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structure, function and evolution of the mitochondrial dividing apparatus

    • Author(s)
      Kuroiwa, T. et al.
    • Journal Title

      Biochimica Biphysica Acta (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] The Structure and Function of Plastids(Robert R.Wise and J.Kenneth Hoober (eds))2006

    • Author(s)
      Miyagishima S. Kuroiwa T.
    • Publisher
      Springer
    • Related Report
      2005 Annual Research Report
  • [Book] 細胞生物学事典2005

    • Author(s)
      石川統, 黒岩常祥, 永田和宏
    • Total Pages
      463
    • Publisher
      朝倉書店
    • Related Report
      2004 Annual Research Report
  • [Publications] Matsuzaki et al.: "Genome sequence of the ultra-small unicellular red alga Cyanidioschyzon merolae 10D"Nature. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyagishima et al.: "Two types of FtsZ proteins in mitochondria and red-lineage chloroplasts : the duplication of FtsZ is implicated in endosymbiosis..."J.Mol.Evol.. 58. 1-13 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyagishima et al.: "An evolutionary puzzle : chloroplast and mitochondrial division rings"Trends in Plant Sci.. 8. 432-438 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyagishima et al.: "A plant-specific dynamin-related protein froms a ring at the chloroplast division site"Plant Cell. 15. 655-665 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohta et al.: "Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae"DNA research. 10. 67-77 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishida et al.: "Dynamic recruitment of Dynamin for final mitochondrial severance in a red alga"Proc.Natl.Acad.Sci.U.S.A.. 100. 2146-2151 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inada, N., Sakai, A., et al.: "Three-dimensional progression of programmed death in the rice coleoptile"Int.Rev.Cytol.. 218. 221-253 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayashi, T., Takahara, et al.: "Detection and localization of a chloroplast-encoded HU-like protein that may organize chloroplast nucleoids"Plant Cell. 14. 1579-1589 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kuroiwa, H., Mori, T., et al.: "Chloroplast division machinary as revealed by immunofluorescence and electron microscopy"Planta. 215. 185-190 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishimura, Y., Misumi, S., et al.: "mt^+ gamate specific nuclease that targets mit-chloroplasts during the sexual reroduction in Chlamydomonas reinhardtii"Genes Dev.. 16. 1116-1128 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishida, K., Takahara, M., et al.: "Dynamic recruitment of daynamin for final mitochondrial severance in a red alga"Proc.Natl.Acad.Sci.USA. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Nozaki, H., Matsuzaki, M., et al.: "The phylogenetic position of red algae revealed by multiple nuclear genes from mitochondria-containig eukaryotes"J.Mol.Evol.. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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