| Project/Area Number |
14205067
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Geotechnical engineering
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| Research Institution | Gifu University |
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Principal Investigator |
SATO Takeshi Gifu University, Faculty of Engineering, Professor, 工学部, 教授 (80135326)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMIZAWA Kazuhiro Gifu University, Faculty of Applied Biological Science, Professor, 応用生物科学部, 教授 (00159005)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥35,750,000 (Direct Cost: ¥27,500,000、Indirect Cost: ¥8,250,000)
Fiscal Year 2004: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2003: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2002: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
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| Keywords | anaerobic microorganism / bio-augmentation / bioremediation / groundwater / advection-dispersion / Michaelis-Menten equation / biodegradation kinetics / numerical simulation / ミカエリスメンテン式 / 数値シミュレーション / Clostridium bifermentans DPH-1 / リアルタイム定量的PCR法 / DNA濃度 / 分散係数 / カラム実験 / 堆積速度 / 遅れ係数 / DNAマイクロアレイ / スタントン数 / ペクレ数 / Two Regionモデル / FISH / 定量的PCR |
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| Research Abstract |
Laboratory column tests confirmed that the transport characteristics of microorganism, Clostridium bifermentans DPH-1 activating tetrachloroethylene (PCE) dechlorination, was well described by a mobile-immobile two-region model (TRM), which has been used in physical transport model of aqueous pollutants through soils. Determined TRM parameters implied that bacterial injection into ground at large amount of concentration is difficult in a rehabilitation manner for widely dispersed contaminated groundwater. Calibrated parameters were carefully evaluated in conjunction with test conditions and features of the soils tested in column. Practical estimation were proposed in transport parameters to simulate bacteria transport and degradation of PCE in bio-augmentation. Biological reaction was identified as first-order kinetics from batch tests where the bacterium strain was mixed with halogenated aliphatic compounds of PCE or TCE (trichloroethylene) as initial pollutant. PCE dechlorination to cis-dichloroethylene (cDCE) via TCE was mathematically described by Monod type function based on Michaelis-Menten equation while the microbial growth rate was not well represented by Monod function. Thus, a relationship between the microbial growth rate and the concentration of PCE and TCE was newly proposed and its appropriateness was verified through a numerical simulation of PCE dechlorination in a fixed-bed reactor column.
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