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Identification and cloning of genes conditioning species-specific parasitism of Magnaporthe oryzae

Research Project

Project/Area Number 14206006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKobe University

Principal Investigator

MAYAMA Shigeyuki  Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (00112251)

Co-Investigator(Kenkyū-buntansha) TOSA Yukio  Kobe University, Graduate School of Science and Technology, Associate Professor, 自然科学研究科, 助教授 (20172158)
NAKAYASHIKI Hitoshi  Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50252804)
PYOYUN Park  Kobe University, Graduate School of Science and Technology, Professor, 自然科学研究科, 教授 (20147094)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥47,190,000 (Direct Cost: ¥36,300,000、Indirect Cost: ¥10,890,000)
Fiscal Year 2004: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2003: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2002: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
KeywordsMagnaporthe / Pyricularia / Mgnaporthe / 非病原力遺伝子 / Magnaporthe oryzae / Magnaporthe grisea / Pyricularia oryzae / Pyricularia grisea / 種特異的寄生性 / 遺伝子対遺伝子説 / コムギ / avirulence gene / blast fungus / AFLP / bulked segregant analysis
Research Abstract

To clarify genetic mechanisms of plant-species specificity of Magnaporthe oryzae, we crossed a Triticum isolate with a Setaria isolate, an Avena isolate, and an Oryza isolate. Using the F_1 progenies derived from these crosses, we identified PWT1, PWT2 (conditioning avirulence of the Setaria isolate on wheat), PWT3, PWT4 (conditioning avirulence of the Avena isolate on wheat), PWT5 (conditioning avirulence of the Oryza isolate on wheat), PFM1, PFM2 (conditioning avirulence of the Triticum isolate on foxtail millet), and PAT1 (conditioning avirulence of the Triticum isolate on oat). We also identified a resistance gene corresponding to PWT4, and designated it as Rwt4. Rwt4 corresponded to PWT4 in a one-to-one manner, suggesting that the plant species specificity of the Triticum/Avena isolates on wheat is conditioned by gene-for-gene interactions. From these avirulence genes, PWT1, PWT4, and PAT1 were chosen as targets for molecular cloning. Using bulked segregant analysis, we found molecular markers that are closely linked to PWT1, PWT4 and PAT1.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (16 results)

All 2005 2003 2002 Other

All Journal Article (9 results) Publications (7 results)

  • [Journal Article] Significance of the PWT4-Rwt4 interaction in the species specificity of Avena isolated of Magnaporthe oryzae on wheat.2005

    • Author(s)
      Kenji HIRATA
    • Journal Title

      Journal of General Plant Pathology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Significance of the PWT4-Rwt4 interaction in the species specificity of Avena isolates of Magnaporthe oryzae on wheat.2005

    • Author(s)
      Hirata, K., Tosa, Y, Nakayashiki, H., Mayama, S.
    • Journal Title

      J.Gen.Plant Pathol (in press)

    • NAID

      10027220237

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Significance of the PWT4-Rwt4 interaction in the species specificity of Avene isolates of Magnaporthe oryzae on wheat.2005

    • Author(s)
      Kenji HIRATA et al.
    • Journal Title

      Journal of General Plant Pathology 71(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analysis of host species specificity of Magnaporthe grisea toward foxtail millet using a genetic cross between isolates from wheat and foxtail millet.2003

    • Author(s)
      Jiro MURAKAMI
    • Journal Title

      Phytopathology 93

      Pages: 42-45

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of host species specificity of Magnaporthe grisca toward foxtail millet using a genetic cross between isolates from wheat and foxtail millet.2003

    • Author(s)
      Murakami, J., R.Tomita, T.Kataoka, H.Nakayashiki, Y.Tosa, S.Mayama
    • Journal Title

      Phytopathology 93

      Pages: 42-45

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A gene-for-gene relationship underlying the species-specific parasitism of Avena/Triticum isolates of Magnaporthe grisea on wheat cultivars.2002

    • Author(s)
      Nobuyoshi TAKABAYASHI
    • Journal Title

      Phytopathology 92

      Pages: 1182-1188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of an Avena isolate of Magnaporthe grisea and identification of a locus conditioning its specificity on oat.2002

    • Author(s)
      Hong Sik OH
    • Journal Title

      Canadian Journal of Botany 80

      Pages: 1088-1095

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A gene-for-gene relationship underlying the species-specific parasitism of Avena/Triticum isolates of Magnaporthe grisea on wheat cultivars.2002

    • Author(s)
      Takabayashi, N., Y.Tosa., H.S.Oh, S.Mayama
    • Journal Title

      Phytopathology 92

      Pages: 1182-1188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of an Avena isolate of Magnaporthe grisea and identification of a locus conditioning its specificity on oat.2002

    • Author(s)
      Oh, H.S., Y.Tosa, N.Takabayashi, S.Nakagawa, R.Tomita, L.D.Don, M.Kusaba, H.Nakayashiki, S.Mayama.(2002)
    • Journal Title

      Can.J.Bot. 80

      Pages: 1088-1095

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Murakami, J. et al.: "Analysis of host species specificity of Magnaporthe grisea toward foxtail millet using a genetic cross between isolates from wheat and foxtail millet."Phytopathology. 第93巻・第1号. 42-45 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tosa, Y. et al.: "Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus"Phytopathology. 第94巻(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Farman, M.L.: "Analysis of the structure of the AVR1-CO39 avirulence locus in virulent rice-infecting isolates of Magnaporthe grisea"Mol. Plant-Microbe Interact.. 15. 6-16 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikeda, K.: "Repeat-induced point mutation (RIP) in Magnaporthe grisea : implications for its sexual cycle in the natural field context"Molecular Microbiology. 45・5. 1355-1364 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takabayashi, N.: "A gene-for-gene relationship underlying the species-specific parasitism of Avena/Triticum isolates of Magnaporthe grisea on wheat cultivars"Phytopathology. 92・11. 1182-1188 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oh, H.S.: "Characterization of an Avena isolate of Magnaporthe grisea and identification of a locus conditioning its specificity on oat"Can. J. Bot.. 80. 1088-1095 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Murakami, J.: "Analysis of host species specificity of Magnaporthe grisea toward foxtail millet using a genetic cross between isolates from wheat and foxtail millet"Phytopathology. 93・1. 42-45 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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