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Forest dynamics study using the methods in molecular ecological genetics

Research Project

Project/Area Number 14206017
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学
Research InstitutionNagoya University

Principal Investigator

YAMAMOTO Shin-ichi  Nagoya University, Trustee, 大学院・生命農学研究科, 理事(研究職) (60191409)

Co-Investigator(Kenkyū-buntansha) TOMARU Nobuhiro  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (50241774)
YOSHIMARU Hiroshi  Forestry and Forest Product Research Institute, Department of Forest Genetics, Head of Ecological Genetic Laboratory, 森林遺伝領域, 生体遺伝研究室長(研究員) (20353914)
SHIMATANI Kenichiro  The Institute of Statistical Mathematics, Department of Statistical Methodology, Assistance Professor, 調査実験解析系, 助手 (70332129)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥53,820,000 (Direct Cost: ¥41,400,000、Indirect Cost: ¥12,420,000)
Fiscal Year 2004: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2003: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2002: ¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000)
Keywordsmolecular ecology / population structure / population dynamics / microsatellite / genetic structure / reproductive system / pollen flow / clonal growth / 遺伝子流動 / 父性解析 / 長期調査プロット / 固体群動態 / 空間自己相関 / 遺伝的変異 / 繁殖成功
Research Abstract

In this research project, we studied the structure and dynamics of tree populations composing forest communities of major forest types in Japan using long-term ecological research sites with relatively large areas and the methods in molecular ecological genetics. The specific objectives were to elucidate population structure and dynamics at individual and DNA levels and reproductive characteristics including modes of reproduction, mating system, seeds and pollen dispersals, and reproductive success of parents, and to explain and predict the population structure and dynamics, and reproductive process by modeling.
1.Population structure and dynamics at the individual level were described for Castanopsis cuspidata var.sieboldii in a warm-temperate evergreen broad-leaved forest, Chamecyparis obtuse, C.pisifera, Thujopsis dolabrata, Quercus crispula, Magnolia obovata, and Betula grossa in a temperate coniferous forest, and Picea jezoensis var.hondoensis, Abies mariesii, Tsuga diversifolia, a … More nd Abies veitchii in a subalpine evergreen coniferous forest.
2.Many microsatellite markers were developed for C.cuspidata var.sieboldii, Fagus crenata, Ilex leucoclada, and M.stellata. The markers were expected to be useful in molecular ecological genetic studies.
3.Genetic structure within populations probably due to limited seed dispersal was described for Camellia japonica, F.crenata, and M.stellat a. The degrees of genetic structure were different between different stages. Half-sib structure of F.crenata saplings was revealed by parentage analysis.
4.Clonal structure within populations was also described for I.leucoclada and C.pisifera. Clonal growth is found to be significant in maintaining and regenerating the populations.
5.In Quercus salicina, the patterns of pollen flow were revealed by paternity analysis. The pollen pools accepted by mother trees were genetically different.
6.Models were constructed to explain and predict the population structure and dynamics at the individual and DNA levels, and the reproductive process. The appropriateness of models was evaluated. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (48 results)

All 2005 2004 2003 2002 Other

All Journal Article (36 results) Publications (12 results)

  • [Journal Article] Fine-scale clonal structure and diversity within patches of a clone-forming dioecious shnub, llex leucoclada(Aquifoliaceae)2005

    • Author(s)
      Torimaru, T.
    • Journal Title

      Annals of Botany 95

      Pages: 295-304

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fine-scale clonal structure and diversity within patches of a clone-forming dioecious shrub, Ilex leucoclada (Aquifoliaceae)2005

    • Author(s)
      Torimaru, T.
    • Journal Title

      Annals of Botany 95

      Pages: 295-304

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fine-scale clonal structure and diversity within patches of a clone-forming dioecious shrub, Ilex leucoclada (Aquifoliaceae).2005

    • Author(s)
      Torimaru, T.
    • Journal Title

      Annals of Botany 95・2

      Pages: 295-304

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dynamics of forest canopy and major tree populations over nine years in a subalpine old-growth coniferous forest, central Japan2004

    • Author(s)
      Miyadokoro, T.
    • Journal Title

      Ecoscience 11

      Pages: 130-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development and characterization of microsatellite markers for Fagus crenata Blume2004

    • Author(s)
      Asuka, Y
    • Journal Title

      Molecular Ecology Notes 4

      Pages: 101-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development and polymorphism of simple sequence repeat DNA markers for the evergreen shrub llex leucoclada M.2004

    • Author(s)
      Torimaru, T.
    • Journal Title

      Molecular Ecology Notes 4

      Pages: 531-533

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Heterogeneous genetic structure in a Fagus crenata population in an old-growth beech forest revealed by microsatellite markers2004

    • Author(s)
      Asuka, Y.
    • Journal Title

      Molecular Ecology 13

      Pages: 1241-1250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Stem distribution and clonal structure of Chamaecyparis pisifera growing in an old-growth beech-conifer forest2004

    • Author(s)
      Hayakawa, T.
    • Journal Title

      Ecological Research 19

      Pages: 411-420

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Size distribution and genetic structure in relation to clonal growth within a population of Magnolia tomentosa Thunb.(Magnoliaceae).2004

    • Author(s)
      Setsuko, S.
    • Journal Title

      Molecular Ecology 13

      Pages: 2645-2653

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Patterns of pollen flow and genetic differentiation among pollen pools in Quercus salicina in a warm temperate old-growth evergreen broad-leaved forest2004

    • Author(s)
      Nakansishi, A.
    • Journal Title

      Silvae Genetica 53

      Pages: 258-264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development and characterization of microsatellite markers for Fagus crenata Blume2004

    • Author(s)
      Asuka, Y.
    • Journal Title

      Molecular Ecology Notes 4

      Pages: 101-103

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development and polymorphism of simple sequence repeat DNA markers for the evergreen shrub Ilex leucoclada M.2004

    • Author(s)
      Torimaru, T.
    • Journal Title

      Molecular Ecology Notes 4

      Pages: 531-533

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Size distribution and genetic structure in relation to clonal growth within a population of Magnolia tomentosa Thunb. (Magnoliaceae)2004

    • Author(s)
      Setsuko, S.
    • Journal Title

      Molecular Ecology 13

      Pages: 2645-2653

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Patterns of pollen flow and genetic differentiation among pollen pools in Quercus salicina in a warm temperate old-growth evergreen broad-leaved forest2004

    • Author(s)
      Nakanishi, A.
    • Journal Title

      Silvae Genetica 53

      Pages: 258-264

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Size distribution and genetic structure in relation to clonal growth within a population of Magnolia tomentosa Thunb. (Magnoliaceae).2004

    • Author(s)
      Setsuko, S
    • Journal Title

      Molecular Ecology 13・9

      Pages: 2645-2653

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dynamics of forest canopy and major tree populations over nine years in a subalpine old-growth coniferous forest, central Japan.2004

    • Author(s)
      Miyadokoro, T.
    • Journal Title

      Ecoscience 11・1

      Pages: 130-136

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of sprouting and canopy states on the structure and dynamics of a Castanopsis cuspidata var.sieboldii sapling population in an old-growth evergreen broad-leaved forest2003

    • Author(s)
      Miura, M.
    • Journal Title

      Forest Ecology and Management 183

      Pages: 387-400

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure and dymamics of Castanopsis cuspidata var.sieboldii population in an old-gowth evergreen broad-leaved forest : the importance of sprout regeneration2003

    • Author(s)
      Miura, M.
    • Journal Title

      Ecological Research 18

      Pages: 115-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of canopy conditions on the regeneration of major tree species in an old-growth Camaecyparis obtusa forest, central Japan2003

    • Author(s)
      Hoshino, D.
    • Journal Title

      Forest Ecology and Management 175

      Pages: 141-152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dynamics of forest canopy and major tree populations over nine years in a subalpine old-growth coniferous forest, central Japan2003

    • Author(s)
      Miyadokoro, T.
    • Journal Title

      Forest Ecology and Management 182

      Pages: 259-272

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A further six microsatellite markers for Castanopsis cuspidata var.sieboldii Nakai2003

    • Author(s)
      Ueno S.
    • Journal Title

      Conservation Genetics 4

      Pages: 813-815

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clonal diversity and genetic differentiation in llex leucoclada M.patches in an old-growth beech forest2003

    • Author(s)
      Torimaru, T.
    • Journal Title

      Molecular Ecology 12

      Pages: 809-818

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of sprouting and canopy states on the structure and dynamics of a Castanopsis cuspidata var. sieboldii sapling population in an old-growth evergreen broad-leaved forest2003

    • Author(s)
      Miura, M.
    • Journal Title

      Forest Ecology and Management 183

      Pages: 387-400

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure and dymamics of Castanopsis cuspidata var.sieboldii population in an old-growth evergreen broad-leaved forest : the importance of sprout regeneration2003

    • Author(s)
      Miura, M.
    • Journal Title

      Ecological Research 18

      Pages: 115-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Population structure and spatial patterns of major trees in a subalpine old-growth coniferous forest, central Japan2003

    • Author(s)
      Miyadokoro, T.
    • Journal Title

      Ecology and Management 182

      Pages: 259-272

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clonal diversity and genetic differentiation in Ilex leucoclada M.patches in an old-growth beech forest2003

    • Author(s)
      Torimaru, T.
    • Journal Title

      Molecular Ecology 12

      Pages: 809-818

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dynamics of major conifer and deciduous broad-leaved tree species in an old-growth Camaecyparis obtusa forest, central Japan2002

    • Author(s)
      Hoshino, D.
    • Journal Title

      Forest Ecology and Management 159

      Pages: 133-144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tree competition and species coexistence in a warm-temperate old-growth evergreen broad-leaved forest in Japan2002

    • Author(s)
      Nishimura, N.
    • Journal Title

      Plant Ecology 164

      Pages: 235-248

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Size-class differences in genetic structure and individual distribution of Camellia japonica L.in a Japanese old-growth evergreen forest2002

    • Author(s)
      Ueno, S.
    • Journal Title

      Heredity 89

      Pages: 120-126

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of microsatellite markers in Magnolia tomentosa Thunb.(Magnoliaceae), a threatened Japanese tree

    • Author(s)
      Setsuko, S.
    • Journal Title

      Conservation Genetics (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Half-sib family structure of Fagus crenata saplings in an old-growth beech-dwarf bamboo forest

    • Author(s)
      Asuka, Y.
    • Journal Title

      Molecular Ecology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of microsatellite markers in Magnolia tomentosa Thunb. (Magnoliaceae), a threatened Japanese tree

    • Author(s)
      Setsuko, S.
    • Journal Title

      Conservation Genetics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Half-sib family structure of Fagus crenata saplings in an old-growth beech-dwarf bamboo forest.

    • Author(s)
      Asuka, Y
    • Journal Title

      Molecular Ecology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Patterns of pollen flow and genetic differentiation among pollen pools in Quercus salicina in a warm temperate old-growth evergreen broad-leaved forest.

    • Author(s)
      Nakanishi, A.
    • Journal Title

      Silvae Genetica (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Half-sib family structure of Fagus crenata sapling in an old-glowth beech-dwarf bamboo forest.

    • Author(s)
      Asuka, Y
    • Journal Title

      Molecular Ecology (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development of microsatellite markers in Magnolia stellata Thunb. (Magnoliaceae), a threatened Japanese tree.

    • Author(s)
      Setsuko, S
    • Journal Title

      Conservation Genetics (in press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Ueno, S.: "A further six microsatellite markers for Castanopsis cuspidata var. sieboldii Nakai"Conservation Genetics. 4・6. 813-815 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asuka, Y: "Development and characterization of microsatellite markers for Fagus crenata Blume"Molecular Ecology Notes. 4・1. 101-103 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asuka, Y.: "Heterogeneous genetic structure in a Fagus crenata population in an old-growth beech forest revealed by microsatellite markers"Molecular Ecology. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayakawa, T.: "Stem distribution and clonal structure of Chamaecyparis pisifera growing in an old-growth beech-conifer forest"Ecological Research. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyadokoro, T: "Population structure and spatial patterns of major trees in a subalpine old-growth coniferous forest, central Japan"Forest Ecology and Management. 182・1-3. 259-272 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujita, T.: "Long-term canopy dynamics analyzed by aerial photographs in a temperate old-growth evergreen broad-leaved forest"Journal of Ecology. 91・4. 686-693 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ueno, S: "Size-class differences in genetic structure and individual distribution of Camellia japonica L. in a Japanese old-growth evergreen forest"Heredity. 89・2. 120-126 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 高橋誠: "遺伝マーカーを用いた樹木集団内遺伝構造の空間解析手法"統計数理. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimatani, K.: "On the methods of spatial analysis for genotyped individuals"Heredity. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Miura, M.: "Structure and dynamics of a Castanopsis cuspidata var. sieboldii population in an old-green broad-leaved forest : the importance of sprout regeneration"Ecological Research. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Torimaru, T.: "Clonal diversity and genetic differentiation in Ilex leucoclada M. patches in an old-growth beech forest on Mt. Daisen, Japan"Molecular Ecology. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujita, T.: "Canopy structure in a temperate old-growth evergreen forest analyzed by using aerial photographs"Plant Ecology. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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