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Analyses on mechanisms of eel oogenesis for the improvement of egg quality

Research Project

Project/Area Number 14206022
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAUCHI Kohei  Hokkaido Univ., Grad.School of Fish.Sci., Prof., 大学院・水産科学研究科, 教授 (10109514)

Co-Investigator(Kenkyū-buntansha) HARA Akihiko  Hokkaido Univ., Grad.School of Fish.Sci., Prof., 大学院・水産科学研究科, 教授 (40091483)
ADACHI Shinji  Hokkaido Univ., Grad.School of Fish.Sci., Asso.Prof., 大学院・水産科学研究科, 助教授 (40231930)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥53,300,000 (Direct Cost: ¥41,000,000、Indirect Cost: ¥12,300,000)
Fiscal Year 2004: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2003: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2002: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
KeywordsJapanese eel / androgen / 11-ketotestosterone / methyltestosterone / oocyte / oil droplet / egg quality / steroidogenic enzyme / アロマターゼ阻害剤 / ビテロゲニン / エストロゲン / 受容体
Research Abstract

The control of 11-ketotestosterone (11KT) biosynthesis and its physiological roles were examined in female eel. Among various tissues, the ovary had the greatest capacity to synthesize 11KT in vitro. In addition, the oocyte diameters of eels treated with 11KT had increased significantly. Furthermore, these oocytes were found to have an increased number of oil droplets. These findings suggest that 11KT appears to play an important role in controlling previtellogenic oocyte growth. In the next, we examined the effects of oral administration of other androgens, 17α-methyltestosterone (MT) on the previtellogenic oocyte growth. Female eels that have oocytes of about 40 μm in diameter were fed the diets containing MT for two and four months. However, significant differences were not observed. In the next, female eels that have oocytes of about 90 μm were fed the diets containing MT. Oocyte diameters in the MT treated group increased with greater abundance of oil droplets. These results indicate that the oral administration of MT can induce growth of previtellogenic oocytes over 90 μm in diameter in eel.
During artificiall maturation, serum estradil-17β(E2) levels in female eel change at relatively lower values till the mid-vitellogenic stage, then elevate linearly to reach maximum levels at even the migratory nucleus stage. Aromatase inhibitor could suppress the higher levels of serum E2.
In this study, we generated and characterized polyclonal antisera against eel P450 cholesterol side-chain cleavage (P450scc), 17α-hydroxylase, aromatase and 11β-hydroxylase using recombinant proteins as antigens. These antisera immunostained specific cells in ovary of eel. Among them, the P450scc antiserum clearly immunostained interrenal steroidogenic cells in head kidney of not only eel but also goldfish, indicating that this antibody are also utilizable for other teleost species.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (31 results)

All 2003 2002 Other

All Journal Article (21 results) Publications (10 results)

  • [Journal Article] Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone a subunit mRNA levels in artificially maturing Japanese eel, Anguilla japonica, and naturally maturing New Zealand longfinned eel, A. dieffenbachii.2003

    • Author(s)
      Saito K.
    • Journal Title

      Fisheries Sci. 69

      Pages: 146-153

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro induction of luteinizing hormone synthesis by estrogen in organ-cultured pituitary glands of the Japanese eel, Anguilla japonica.2003

    • Author(s)
      Saito K.
    • Journal Title

      Zool.Sci. 20

      Pages: 69-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ovarian mitochondrial cytochrome b mRNA levels increase with sexual maturity in freshwater eels (Anguilla spp.)2003

    • Author(s)
      Lokman P.M.
    • Journal Title

      J Comp.Physiol.B 173

      Pages: 11-19

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of a cDNA encoding P-450 aromatase (CYP19) from Japanese eel ovary and its expression in ovarian follicles during induced ovarian development.2003

    • Author(s)
      Ijiri S.
    • Journal Title

      Gen.Comp.Endocrinol. 130

      Pages: 193-203

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in mRNA levels of ovarian steroidogenic enzymes during artificial maturation of Japanese eel Anguilla japonica.2003

    • Author(s)
      Matsubara H.
    • Journal Title

      Fisheries Sci. 69

      Pages: 979-988

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterization of 3 β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase cDNAs from Japanese eel ovary.2003

    • Author(s)
      Kazeto Y.
    • Journal Title

      J.Steroid.Biochem.Mol.Biol. 85

      Pages: 49-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in immunolocalization of steroidogenic enzymes (P450scc, P450cl7, P450arom) in gonad of Japanese eel2003

    • Author(s)
      Ijiri S.
    • Journal Title

      Fish Physiol.Biochem. 28

      Pages: 209-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis and possible function of 11-ketotestosterone during oogenesis in eel (Anguilla spp.)2003

    • Author(s)
      Matsubara H.
    • Journal Title

      Fish Physiol.Biochem. 28

      Pages: 353-354

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 11-Ketotestosterone potentiates estrogen-induced vitellogenin production in liver of Japanese eel (Anguilla japonica)2003

    • Author(s)
      Asanuma H.
    • Journal Title

      Fish Physiol.Biochem. 28

      Pages: 383-384

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Maturational factors as indicators of egg quality in Japanese eel (Anguilla japonica)2003

    • Author(s)
      Shin D.H.
    • Journal Title

      Fish Physiol.Biochem. 28

      Pages: 519-520

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serum steroid profiles in artificially maturing female Japanese eel, Anguilla japonica2003

    • Author(s)
      Matsubara H.
    • Journal Title

      Aquaculture 243

      Pages: 393-402

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone α subunit mRNA levels in artificially maturing Japanese eel, Anguilla japonica, and naturally maturing New Zealand longfinned eel, A.dieffenbachii.2003

    • Author(s)
      Saito K.
    • Journal Title

      Fisheries Sci. 69

      Pages: 146-153

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ovarian mitochondrial cytochrome b mRNA levels increase with sexual maturity in freshwater eels (Anguilla spp.).2003

    • Author(s)
      Lokman P.M.
    • Journal Title

      J.Comp.Physiol.B. 173

      Pages: 11-19

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterization of 3β-hydroxysteroid dehydrogenase/ Δ5-Δ4 isomerase cDNAs from Japanese eel ovary.2003

    • Author(s)
      Kazeto Y.
    • Journal Title

      J.Steroid.Biochem.Mol.Biol. 85

      Pages: 49-56

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in immunolocalization of steroidogenic enzymes (P450scc, P450c17, P450arom) in gonad of Japanese eel.2003

    • Author(s)
      Ijiri S.
    • Journal Title

      Fish Physiol.Biochem. 28

      Pages: 209-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis and possible function of 11-ketotestosterone during oogenesis in eel (Anguilla spp.).2003

    • Author(s)
      Matsubara H.
    • Journal Title

      Fish Physiol.Biochem. 28

      Pages: 353-354

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in hepatic vitelligenin mRNA levels during oocyte development in the Japanese eel, Anguilla japonica2002

    • Author(s)
      Okumura H.
    • Journal Title

      Gen.Comp.Endocrinol. 125

      Pages: 9-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in mRNA levels of vitellogenin 1 and 2 during artificial maturation in Japanese eel, Anguilla japonica2002

    • Author(s)
      Matsubara H.
    • Journal Title

      Fisheries Sci. 68(supl.)

      Pages: 971-972

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 11-Oxygenated androgens in female teleosts : prevalence, abundance, and life history implications2002

    • Author(s)
      Lokman P.M.
    • Journal Title

      Gen.Comp.Endocrinol. 129

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation of two types of cDNA encoding growth hormone receptor-like genes in the Japanese eel, Anguilla japonica2002

    • Author(s)
      Ozaki Y.
    • Journal Title

      Fisheries Sci. 68(supl.)

      Pages: 959-960

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in mRNA levels of vitellogenin 1 and 2 during artificial maturation in Japanese eel, Anguilla japonica2002

    • Author(s)
      Matsubara H
    • Journal Title

      Fisheries Sci. 68(supl.)

      Pages: 971-972

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Saito K.: "Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone α subunit mRNA levels in artificially maturing Japanese eel, Anguilla japonica, and naturally maturing New Zealand longfinned eel, A. dieffenbachii"Fisheries Sci.. 69. 146-153 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Saito K.: "In vitro induction of luteinizing hormone synthesis by estrogen in organ-cultured pituitary glands of the Japanese eel, Anguilla_japonica"Zool.Sci.. 20. 69-73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Lokman P.M.: "Ovarian mitochondrial cytochrome b mRNA levels increase with sexual maturity in freshwater eels (Anguilla spp.)"J.Comp.Physiol.B. 173. 11-19 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ijiri S.: "Characterization of a cDNA encoding P-450 aromatase (CYP19) from Japanese eel ovary and its expression in ovarian follicles during induced ovarian development"Gen.Comp.Endocrinol.. 130. 193-203 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsubara H.: "Changes in mRNA levels of ovarian steroidogenic enzymes during artificial maturation of Japanese eel Anguilla japonica"Fisheries Sci.. 69. 979-988 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kazeto Y.: "Molecular cloning and characterization of 3 β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase cDNAs from Japanese eel ovary"J.Steroid.Biochem.Mol.Biol.. 85. 49-56 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okumura H.: "Changes in hepatic vitelligenin mRNA levels during oocyte development in the Japanese eel, Anguilla japonica"Gen. Comp. Endocrinol,. 125. 9-16 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsubara H.: "Changes in mRNA levels of vitellogenin 1 and 2 during artificial maturation in Japanese eel, Anguilla japonica"Fisheries Sci.. 68(supl.). 971-972 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Lokman P.M.: "11 -Oxygenated androgens in female teleosts : prevalence, abundance, and life history implications"Gen. Comp. Endocrinol.. 129. 1-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ozaki Y.: "Isolation of two types of cDNA encoding growth hormone receptor-like genes in the Japanese eel, Anguilla japonica"Fisheries Sci.. 68(supl.). 959-960 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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