| Project/Area Number |
14206024
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
OGAWA Kazuo The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (20092174)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIZAWA Toyohiko Hokkaido University, Graduate School of Fisheries Sciences, Associate Professor, 大学院・水産科学研究科, 助教授 (10222184)
NAKAI Toshihiro Hiroshima University, Graduate School of Biosphere Sciences, Professor, 大学院・生物圏科学研究科, 教授 (60164117)
MUROGA Kiyokuni Tohoku University, Graduate School of Agriculture, Professor, 大学院・農学研究科, 教授 (30011993)
YOKOYAMA Hiroshi The University of Tokyo, Graduate School of Agricultural and Life Sciences, assistant, 大学院・農学生命科学研究科, 助手 (70261956)
YOSHINAGA Tomoyoshi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20345185)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2003: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2002: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000)
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| Keywords | emerging disease / Neoheterobothrium / birnavirus / cold water disease / white spot disease / microsporidium / wild fish / cultured fish |
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| Research Abstract |
1.Field investigation of the flounder birnavirus and VHSV among wild marine fishes showed that aquabirnavirus was isolated from horse mackerel, Japanese flounder and dark banded rockfish, and that VHSV was detected from Japanese flounder and sand lance. All isolates of VHSV except one belonged to Genogroup 3 (American genogroup). VHSV was easily transmitted by water-borne route from infected to uninfected fish, suggesting that the infection cycle completed between wild and cultured flounder.
2.Occurrences of Neoheterobothrium hirame in 0-and 1-year-old Japanese flounder in Miyako Bay, Iwate Prefecture and Obama Bay, Fukui Prefecture were surveyed. In both areas, infection levels in both wild and released flounders were high in winter and low in summer and were several fold higher in Obama than in Miyako.
3.Twenty three isolates of Flavobacterium psychrophilum, the causative bacterium of cold-water disease, were found in wild freshwater fishes (crucian carp, dace and ayu). We tried to apply the 5 isolates of bacteriolytic phages from ayu farms as biological control of cold-water disease.
4.Sedimentation velocity and time required for the cyst formation of the protomont of Cryptocaryon irritans was determined. The determined values indicate that the parasite cyst is formed on the sea bed and the infection cycle of the parasite occurs between the sea bottom and the net-cage. Furthermore, it is suggested that the outbreak of the parasite is related to tide.
5.Development of Kabatana takedai was suppressed by low temperature, but recurred by increase of temperature, suggesting that a release of latently infected fish results in spread of the disease. The unknown infective stage, ca. 40μm in size, was shown to originate in the dam located in an upper stream of the Chitose hatchery and invade fish perorally.
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