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Studies on infection cycles of newly emerging and re-emerging diseases of fishes

Research Project

Project/Area Number 14206024
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

OGAWA Kazuo  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (20092174)

Co-Investigator(Kenkyū-buntansha) NISHIZAWA Toyohiko  Hokkaido University, Graduate School of Fisheries Sciences, Associate Professor, 大学院・水産科学研究科, 助教授 (10222184)
NAKAI Toshihiro  Hiroshima University, Graduate School of Biosphere Sciences, Professor, 大学院・生物圏科学研究科, 教授 (60164117)
MUROGA Kiyokuni  Tohoku University, Graduate School of Agriculture, Professor, 大学院・農学研究科, 教授 (30011993)
YOKOYAMA Hiroshi  The University of Tokyo, Graduate School of Agricultural and Life Sciences, assistant, 大学院・農学生命科学研究科, 助手 (70261956)
YOSHINAGA Tomoyoshi  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20345185)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2003: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2002: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000)
Keywordsemerging disease / Neoheterobothrium / birnavirus / cold water disease / white spot disease / microsporidium / wild fish / cultured fish
Research Abstract

1.Field investigation of the flounder birnavirus and VHSV among wild marine fishes showed that aquabirnavirus was isolated from horse mackerel, Japanese flounder and dark banded rockfish, and that VHSV was detected from Japanese flounder and sand lance. All isolates of VHSV except one belonged to Genogroup 3 (American genogroup). VHSV was easily transmitted by water-borne route from infected to uninfected fish, suggesting that the infection cycle completed between wild and cultured flounder.
2.Occurrences of Neoheterobothrium hirame in 0-and 1-year-old Japanese flounder in Miyako Bay, Iwate Prefecture and Obama Bay, Fukui Prefecture were surveyed. In both areas, infection levels in both wild and released flounders were high in winter and low in summer and were several fold higher in Obama than in Miyako.
3.Twenty three isolates of Flavobacterium psychrophilum, the causative bacterium of cold-water disease, were found in wild freshwater fishes (crucian carp, dace and ayu). We tried to apply the 5 isolates of bacteriolytic phages from ayu farms as biological control of cold-water disease.
4.Sedimentation velocity and time required for the cyst formation of the protomont of Cryptocaryon irritans was determined. The determined values indicate that the parasite cyst is formed on the sea bed and the infection cycle of the parasite occurs between the sea bottom and the net-cage. Furthermore, it is suggested that the outbreak of the parasite is related to tide.
5.Development of Kabatana takedai was suppressed by low temperature, but recurred by increase of temperature, suggesting that a release of latently infected fish results in spread of the disease. The unknown infective stage, ca. 40μm in size, was shown to originate in the dam located in an upper stream of the Chitose hatchery and invade fish perorally.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Watanabe et al.: "Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VHSV) from wild marine fishes"Fish Pathology. 37. 189-191 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishizawa et al.: "Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus (VHSV) based on partial G and P genes."Diseases of Aquatic Organisms. 48. 143-148 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mori et al.: "Properties of viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder Paralichthys olivaceus."Fish Pathology. 37. 164-174 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiyama et al.: "Investigation of the transmission stage of the microsporidian Kabatana takedai in salmonids."Bulletin of National Salmon Resources Center. 5. 1-6 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pakingking, R.Jr.et al.: "Experimental coinfection with aquabirnavirus and viral hemorrhagic septicemia virus (VHSV), Edwardsiella tarda or Streptococcus iniae in Japanese flounder Paralichthys olivaceus."Fish Pathology. 38. 15-21 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 飯田秀貴ら: "浸漬法により感染させたヒラメにおけるウイルス性出血性敗血症ウイルスの消長"魚病研究. 38. 87-91 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Muroga, K.et al.: "Experimental horizontal transmission of viral hemorrhagic septicemia virus (VHSV) in Japanese flounder Paralichthys olivaceus."Diseases of Aquatic Organisms. 58. 111-115 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pakingking, R.Jr.et al.: "In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus."Fish & Shellfish Immunology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Watanabe et al.: "Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VIISV) from wild marine fishes"Fish Pathology. 37(4). 189-191 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishizawa et al.: "Genetic relatedness among Japanese American and European isolates of viral hemorrhagic Septicemia virus (VHSV) based on partial G and P genes."Diseases of Aquatic Organism. 48(2). 143-148 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mori et al.: "Properties of viral hemourhagic septicemia virus (VHSV) isolated from Japanese flounder Paralichthys olivaceus."Fish Pathology. 37(4). 164-174 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiyama et al.: "Investigation of the transmission stage of the microsporidian Kabatana takedai in salmonids."Bull.Natl.Salmon Res.Center. 5. 164-174 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pakingking, R.Jr. et al.: "Experimental coinfection with aquabirnavirus and viral hemorrhagic septicemia virus (VHSV), Edwardsiella tarda or Streptococcus iniae in Japanese flounder Paralichthys olivaceus."Fish Pathology. 38(1). 15-21 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iida et al.: "Fate of viral hemorrhagic septicemia virus in Japanese flounder Paralichthys olivaceus challenged by immersion."Fish Pathology. 38(3). 87-91 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Muroga et al.: "Experimental horizontal transmission of viral hemorrhagic septicemia virus (VHSV) in Japanese flounder Paralichihys olivaceus."Diseases of Aquatic Organisms. 58(2-3). 111-115 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pakingking, R.Jr. et al.: "In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus."Fish & Shellfish Immunology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pakingking, R.Jr.et al.: "Experimental coinfection with aquabirnavirus and viral hemorrhagic septicemia virus (VHSV), Edwardsiella tarda or Streptococcus iniae in Japanese flounder Paralichthys olivaceus."Fish Pathology. 38. 15-21 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Pakingking, R.Jr.et al.: "In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus"Fish & Shellfish Immunology. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 飯田秀貴: "浸漬法により感染させたヒラメにおけるウイルス性出血性敗血症ウイルスの消長"魚病研究. 38. 87-91 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Muroga, K.et al.: "Experimental horizontal transmission of viral hemorrhagic septicemia virus (VHSV) in Japanese flounder Paralichthys olivaceus."Diseases of Aquatic Organisms. 58. 111-115 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe et al.: "Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VHSV) from wild marine fishes"Fish Pathology. 37巻4号. 189-191 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishizawa et al.: "Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus (VHSV) based on partial G and P genes"Diseases of Aquatic Organisms. 48巻2号. 143-148 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mori et al.: "Properties of viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder Paralichthys olivaceus"Fish Pathology. 37巻4号. 164-174 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujiyama et al.: "Investigation of the transmission stage of the microsporidian Kabatana takedai in salmonids"Bulletin of National Salmon Resources Center. 5号. 1-6 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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