| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Katsuyasu Nagasaki University, Faculty of Fisheries, Professor, 水産学部, 教授 (20171712)
TAKATANI Tomohiro Nagasaki University, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (90304972)
NOGUCHI Tamao Nagasaki University, Faculty of Fisheries, Technical Advisor, 技術顧問 (40011910)
TANIGAWA Akio Cooperative Association for Fish Processing Complex in Nagasaki Fishing Port, Executive Director, Chief Manufacturing Researcher, 専務理事/研究製造部長
MAEKAWA Shuji Nagasaki Municipal Fisheries Center, Head Officer, 所長
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| Budget Amount *help |
¥53,300,000 (Direct Cost: ¥41,000,000、Indirect Cost: ¥12,300,000)
Fiscal Year 2004: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2003: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2002: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
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| Research Abstract |
Assuming the practical use of "puffer culture using puffer toxin (tetrodotoxin ; TTX)", the tiger puffer Takifugu rubripes (larvae, 1-year-, or 2-years-old fish) were reared with several. types of TTX-containing diets in an actual culture field or in the similar conditions, and investigated for their toxin accumulation, health conditions, immunofunctions, etc. As the results, potentiation of both antibody productivity and splenocyte proliferation reaction were seen in the test fish administered with TTX irrespective of their age, indicating that the feeding of TTX-containing diets could be effective for growing up healthy puffers also in practical scales. When the puffers were administered with paralytic shellfish poison (PSP), they could accumulate it less efficiently than TTX, where no immunopotentiating effect was recognized.
Two types of TTX, purified and crude, were injected intraperitoneally into T.rubripes. Both the toxins administered moved mainly into skin and liver, though the
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purified one seemed to move more rapidly, while the crude one was accumulated much more densely. In addition, change in splenocyte proliferation reaction correlating with the movement of TTX was observed.
Micro-distribution of TTX in the tissues of TTX-bearing organisms was visualized by an immuno-enzymatic technique. In pufferfishes, TTX was distributed mainly in basal cells and succiform cells of the skin, yolk vesicles of oocytes, lysosomes of the basal cells, etc., although the distribution pattern varies depending on the species. In case of the ribbon worm Cephalothrix sp., TTX was recognized in the vesicles apically arranged in the bacillary cells, the granular cells in the proboscis, rhynchocoel epithelium, etc., whereas in the flatworm Planocera reticulata, only in the ovum.
In addition to the above results, it could also be clarified that the marine puffer Arothron firmamentum had a considerable amount of PSP in the matured ovary, and that the T.rubripes cultured with non-toxic diet was completely non-toxic. Less
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