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Molecular biological characterization and hypoallergenicity of seafood allergens

Research Project

Project/Area Number 14206026
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Marine Science and Technology (2004)
東京水産大学 (2002-2003)

Principal Investigator

SHIOMI Kazuo  Tokyo University of Marine Science and Technology, Department of Food Science and Technology, Professor, 海洋科学部, 教授 (90111690)

Co-Investigator(Kenkyū-buntansha) HACHIMURA Satoshi  University of Tokyo, Graduate School of Agricultural and life Science, Associate Professor, 大学院・農学生命科学研究科, 助教授 (40238019)
USHIO Hideki  Tokyo University of Marine Science and Technology, Department of Food Science and Technology, Associate Professor, 助教授 (50251682)
ISHIZAKI Shoichiro  Tokyo University of Marine Science and Technology, Department of Food Science and Technology, Assistant Professor, 助手 (40251681)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥52,000,000 (Direct Cost: ¥40,000,000、Indirect Cost: ¥12,000,000)
Fiscal Year 2004: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2003: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2002: ¥29,900,000 (Direct Cost: ¥23,000,000、Indirect Cost: ¥6,900,000)
Keywordsseafood / allergenicity / parvalbumin / collagen / tropomyosin / cDNA cloning / monoclonal antibody / epitope / パルプアルブミン / IgE結合エピトープ / T細胞エピトープ / 魚貝類 / アレルギー発症モデルマウス / 魚類 / lgE結合エピトープ
Research Abstract

1.Parvalbumin(PA) was purified from nine species of fish and the edible frog and demonstrated to be a major allergen in all species. Primary structures of PA from eight species of fish were elucidated by a cDNA cloning technique. Recombinant mackerel PA expressed in E. coli was shown to be comparable in IgE binding ability to native PA.
2.Collagens from various species of fish were confirmed to be allergenic. In the case of rainbow trout and mackerel, a chains separated were shown to be all allergenic and cross-reactive. Five overlapping proteins that cover the entire sequence of rainbow trout α2 chain were individually expressed in E. coli and the recombinant protein corresponding to the C-terminal region of the α2 chain was found to contain important IgE binding epitopes.
3.Tropomyosin(Tm) was established to be a major and cross-reactive allergen in common with various species of crustaceans and cephalopods. Primary structures of Tm from five species of crustaceans and five species of cephalopods were determined by cDNA cloning.
4.Monoclonal and polyclonal antibodies against crustacean and molluscan Tm were produced and shown to be applicable to specific analysis of crustacean Tm by sandwich ELISA. The surface plasmon resonance method using monoclonal antibodies raised against fish PA was useful to determine PA in seafood with high sensitivity.
5.Allergen-responsive Th1 cells were obtained from BALB/c mice immunized with either mackerel PA or American lobster Tm. Analysis of the Th1 cell response to synthetic overlapping peptides revealed that T cell epitopes of mackerel PA are contained in peptides 11-25,26-40 and 56-75 and those of American lobster Tm in peptides 146-160,196-210 and 226-250.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (58 results)

All 2005 2004 2003 2002 Other

All Journal Article (42 results) Book (4 results) Publications (12 results)

  • [Journal Article] Allergenicity of crustacean extractives and reduction by protease digestion2005

    • Author(s)
      Kuniyoshi Shimakura
    • Journal Title

      Food Chem. 91(2)

      Pages: 247-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Allergenicity of crustacean extractives and its reduction by protease digestion.2005

    • Author(s)
      K.Shimakura, Y.Tonomura, Y., Y.Nagashima, K.Shiomi
    • Journal Title

      Food Chem. 91(2)

      Pages: 247-253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Allergenicity of crustacean extractives and its reduction by protease digestion2005

    • Author(s)
      K.Shimakura
    • Journal Title

      Food Chem. 91(2)

      Pages: 247-253

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin2004

    • Author(s)
      Yuki Hamada
    • Journal Title

      Allergol.Int. 53(3)

      Pages: 271-278

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reactivity of serum immunoglobulin E to bullfrog Rana catesbeiana parvalbumins in fish-allergic patients2004

    • Author(s)
      Yuki Hamada
    • Journal Title

      Fish.Sci. 70(6)

      Pages: 1137-1143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification and molecular cloning of a major allergen from Anisakis simplex2004

    • Author(s)
      Kuniyoshi Shimakura
    • Journal Title

      Mol.Biochem.Parasitol. 135(1)

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rapid detection of fish major allergen parvalbumin by surface plasmon resonance biosensor2004

    • Author(s)
      Ying Lu
    • Journal Title

      J.Food Sci. 69(8)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oral administration of food antigen induces T cell mediated intestinal inflammation : A model using TCR-transgenic mice2004

    • Author(s)
      Akira Kikuchi
    • Journal Title

      Bifactors 21

      Pages: 159-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 水産食品のアレルギー問題2004

    • Author(s)
      塩見 一雄
    • Journal Title

      日水誌 70(5)

      Pages: 806-807

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Preparation and characterization of monoclonal antibody against abalone allergen tropomyosins2004

    • Author(s)
      Ying Lu
    • Journal Title

      Hybridoma Hybridomics 23(6)

      Pages: 357-361

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin.2004

    • Author(s)
      Y.Hamada, H.Tanaka, A.Sato, S.Ishizaki, Y.Nagashima, K.Shiomi
    • Journal Title

      Allergol.Int. 53(3)

      Pages: 271-278

    • NAID

      10014175966

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reactivity of serum immunoglobulin E to bullfrog Rana catesbeiana parvalbumins in fish-allergic patients.2004

    • Author(s)
      Y.Hamada, Y.Nagashima, K.Shiomi
    • Journal Title

      Fish.Sci. 70(6)

      Pages: 1137-1143

    • NAID

      10013706482

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification and molecular cloning of a major allergen from Anisakis simplex.2004

    • Author(s)
      K.Shimakura, H.Miura, K.Ikeda, S.Ishizaki, Y.Nagashima, T.Shirai, S.Kasuya, K.Shiomi
    • Journal Title

      Mol.Biochem.Parasitol. 135(1)

      Pages: 69-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rapid detection of fish major allergen parvalbumin by surface plasmon resonance biosensor.2004

    • Author(s)
      Y.Lu, T.Ohshima, H.Ushio
    • Journal Title

      J.Food Sci. 69(8)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oral administration of food antigen induces T cell mediated intestinal inflammation : A model using TCR-transgenic mice.2004

    • Author(s)
      A.Kikuchi, H.Nakajima-Adachi, A.Ebihara, Y.Takahashi, A.Hosono, K.Itoh, S.Hachimura, S.Kaminogawa
    • Journal Title

      Biofactors 21

      Pages: 159-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seafood allergy.2004

    • Author(s)
      K.Shiomi
    • Journal Title

      Nippon Suisan Gakkaishi 70(5)

      Pages: 806-807

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Preparation and characterization of monoclonal antibody against abalone allergen tropomyosins.2004

    • Author(s)
      Y.Lu, H.Ushio, T.Ohshima, H.Yamanaka, Y.Hamada, K.Shiomi
    • Journal Title

      Hybridoma Hybridomics 23(6)

      Pages: 357-361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rapid detection of fish major allergen parvalbumin by surface plasmon resonance biosensor2004

    • Author(s)
      Y.Lu
    • Journal Title

      J.Food Sci. 69

      Pages: 652-658

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Oral administration of food antigen induces T cell mediated intestinal inflammation : A model using TCR-transgenic mice2004

    • Author(s)
      A.Kikuchi
    • Journal Title

      Biofactors 21

      Pages: 159-162

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Purification and molecular cloning of a major allergen from Anisakis simplex2004

    • Author(s)
      K.Shimakura
    • Journal Title

      Mol.Biochem.Parasitol. 135(1)

      Pages: 69-75

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin2004

    • Author(s)
      Y.Hamada
    • Journal Title

      Allergol.Int. 53(3)

      Pages: 271-278

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Reactivity of IgE in fish-allergic patients to a and b-parvalbumins purified from the bullfrog Rana catesbeiana2004

    • Author(s)
      Y.Hainada
    • Journal Title

      Fish.Sci. 70(6)

      Pages: 1137-1143

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Preparation and characterization of monoclonal antibody against abalone allergen tropomyosin2004

    • Author(s)
      Y.Lu
    • Journal Title

      Hybridoma and Hybridomics 23(6)

      Pages: 357-361

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 水産食品のアレルギー問題2004

    • Author(s)
      塩見一雄
    • Journal Title

      日水誌 70(5)

      Pages: 806-807

    • Related Report
      2004 Annual Research Report
  • [Journal Article] アトピー性皮膚炎に合併した魚類によるOral allergy syndromeの1例2003

    • Author(s)
      松倉節子
    • Journal Title

      アレルギーの臨床 23(1)

      Pages: 74-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification, reactivity with IgE and cDNA cloning of parvalbumin as the major allergen of mackerels2003

    • Author(s)
      Yuki Hamada
    • Journal Title

      Food Chem.Toxicol. 41(8)

      Pages: 1149-1156

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reactivity of IgE in fish-allergic patients to fish muscle collagen2003

    • Author(s)
      Yuki Hamada
    • Journal Title

      Allergol.Int. 52(3)

      Pages: 139-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 食物負荷試験用に製造した乾燥食品粉末のアレルゲン性のin vitroによる評価2003

    • Author(s)
      嶋倉邦嘉
    • Journal Title

      アレルギー 52(6)

      Pages: 522-529

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 魚類アレルギー患者血清のカエルパルブアルブミンに対する反応性2003

    • Author(s)
      濱田友貴
    • Journal Title

      食物アレルギー研究会会誌 3

      Pages: 83-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 魚介類とアレルギー2003

    • Author(s)
      塩見一雄
    • Journal Title

      アレルギーの臨床 23(14)

      Pages: 33-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Antigen feeding enhances frequency and antigen-specific proliferation ability of intraepithelial CD4^+ T cells in αβ T cell receptor transgenic mice2003

    • Author(s)
      Masao Goto
    • Journal Title

      Biosci.Biotechnol.Biochem. 67(6)

      Pages: 1223-1229

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteome analysis reveals caspase activation in hyporesponsive CD4 T lymphocytes induced in vivo by the oral administration of antigen2003

    • Author(s)
      Tomohiro Kaji
    • Journal Title

      J.Biol.Chem. 278(30)

      Pages: 27836-27843

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oral allergy syndrome due to many kinds of fish associated with severe atopic dermatitis.2003

    • Author(s)
      S.Matukura, Megumi Kondo, Hiroyuki Osuna, Zenro Ikezawa, Kazuo Shiomi
    • Journal Title

      The Allergy in Practice 23(1)

      Pages: 74-77

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro evaluation of allergenicity of dried food powders manufactured for food provocation test.2003

    • Author(s)
      K.Shimakura, Y.Nagashima, K.Shiomi, M.Kunou, M.Ebisawa, A.Akasawa, Y.Iikura
    • Journal Title

      Arerugi 52(6)

      Pages: 522-529

    • NAID

      110002404114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reactivity of IgE in fish-allergic patients to frog parvalbumins.2003

    • Author(s)
      Y.Hamada, K.Shiomi
    • Journal Title

      Shokumotsu-arerugi Kenkyukai-shi 3

      Pages: 83-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification, reactivity with IgE and cDNA cloning of parvalbumin as the major allergen of mackerels.2003

    • Author(s)
      Y.Hamada, H.Tanaka, S.Ishizaki, M.Ishida, Y.Nagashima, K.Shiomi
    • Journal Title

      Food Chem.Toxicol. 41(8)

      Pages: 1149-1156

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reactivity of IgE in fish-allergic patients to fish muscle collagen.2003

    • Author(s)
      Y.Hamada, Y.Nagashima, K.Shiomi, N.Shimojo, Y.Kohno, R.Shibata, S.Nishima, H.Ohsuna, Z.Ikezawa
    • Journal Title

      Allergol.Int. 52(3)

      Pages: 139-147

    • NAID

      130004476784

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seafood allergy.2003

    • Author(s)
      K.Shiomi
    • Journal Title

      The Allergy in Practice 23(14)

      Pages: 33-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Antigen feeding enhances frequency and antigen-specific proliferation ability of intraepithelial CD4^+ T cells in αβ T cell receptor transgenic mice.2003

    • Author(s)
      M.Goto, S.Hachimura, A.Ametani, T.Sato, Y.Kumagai, S.Habu, M.Totsuka, H.Ishikawa, S.Kaminogawa
    • Journal Title

      Biosci.Biotechnol.Biochem. 67(6)

      Pages: 1223-1229

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteome analysis reveals caspase activation in hyporesponsive CD4 T lymphocytes induced in vivo by the oral administration of antigen.2003

    • Author(s)
      T.Kaji, S.Hachimura, W.Ise, S.Kaminogawa
    • Journal Title

      J.Biol.Chem. 278(30)

      Pages: 27836-27843

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 魚貝類アレルギー問題と研究の現状2002

    • Author(s)
      塩見一雄
    • Journal Title

      New Food Industry 44(12)

      Pages: 33-40

    • NAID

      40005468968

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Current knowledge on seafood allergy.2002

    • Author(s)
      K.Shiomi
    • Journal Title

      New Food Industry 44(12)

      Pages: 33-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 魚介類のアレルギー問題.水産学シリーズ143 水産食品の安全・安心対策-現状と課題(阿部宏基・内田直行編)2004

    • Author(s)
      塩見一雄
    • Total Pages
      14
    • Publisher
      恒星社厚生閣
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Seafood allergy. Current situation and problems on the safety and reliability of seafood (Edited by H.Abe and N.Uchida).2004

    • Author(s)
      K.Shiomi
    • Publisher
      KOSEISHA-KOSEIKAKU,
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 魚貝類とアレルギー2003

    • Author(s)
      塩見一雄
    • Total Pages
      164
    • Publisher
      成山堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Seafood allergy.(Verseau Books 013).2003

    • Author(s)
      K.Shiomi
    • Publisher
      SEIZANDO
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 松倉節子: "アトピー性皮膚炎に合併した魚類によるOral allergy syndromeの1例"アレルギーの臨床. 23(1). 74-77 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuki Hamada: "Purification, reactivity with IgE and cDNA cloning of parvalbumin as the major allergen of mackerels"Food Chem.Toxicol.. 41(8). 1149-1156 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuki Hamada: "Reactivity of IgE in fish-allergic patients to fish muscle collagen"Allergol.Int.. 52(3). 139-147 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 濱田友貴: "魚類アレルギー患者血清のカエルパルブアルブミンに対する反応性"食物アレルギー研究会会誌. 3. 83-86 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuniyoshi Shimakura: "Allergenicity of crustacean extractives and its reduction by protease digestion"Food Chem.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuniyoshi Shimakura: "Purification and molecular cloning of a major allergen from Anisakis simplex"Mol.Biochem.Parasitol.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masao Goto: "Antigen feeding enhances frequency and antigen-specific proliferation ability of intraepithelial CD4^+ T cells in αβ T cell receptor transgenic mice"Biosci.Biotechnol.Biochem.. 67(6). 1223-1229 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomohiro Kaji: "Proteome analysis reveals caspase activation in hyporesponsive CD4 T lymphocytes induced in vivo by the oral administration of antigen"J.Biol.Chem.. 278(30). 27836-27843 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 塩見一雄: "魚貝類とアレルギー"成山堂. 164 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 松倉節子: "アトピー性皮膚炎に合併した魚類によるOral allergy syndromeの1例"アレルギーの臨床. 23(1). 74-77 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuki Hamada: "Purification, reactivity with lgE and cDNA cloning of parvalbumin as the major allergen of mackerels"Food Chem.Toxicol.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuki Hamada: "Reactivity of lgE in fish-allergic patients to fish muscle collagn"Allergol.Int.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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