| Project/Area Number |
14206031
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Iwate University |
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Principal Investigator |
SAIGA Suguru Iwate University, Faculty of Agriculture, Professor, 農学部, 教授 (10183360)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Haruo Iwate University, Faculty of Agriculture, Professor, 農学部, 教授 (60003779)
NAKASHIMA Hiroshi Hokkaido University, Emeritus Professor, 名誉教授 (40001474)
INOUE Tatsushi Miyagi Prefectural university, Lecturer, 講師 (20264351)
SUGAWARA Kohya National Institute of Livestock and Grassland Science, Researcher, 研究員 (30355078)
YAMASITA Masayuki Shizuoka University, Faculty of Agriculture, Associate Professor, 助教授 (30252167)
佐々木 亨 飼料作物研究所, 主任研究員
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2005: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2004: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2003: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2002: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | Alkaloid / Endophyte / Minerals / Non-toxic / Perennial ryegrass / Rotarod / Symbiosis / Tall fescue / ニュージーランド / 水耕培養 / 国際研究者交流 |
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| Research Abstract |
The aim of this research project was to find non-toxic endophyte strains adapted to cold and wet climate in northern Japan and to study for the practical use. For this purpose, we systematically collected perennial ryegrass and tall fescue plants infected with Neotyphodium endophytes. Among them a perennial ryegrass plant collected from Fukaura, Aomori prefecture was low in ergovaline and lolitrem B contents which were toxic to livestock. Endophyte fungi of Fukaura perennial ryegrass ecotype were morphologically similar to wild type endophyte and identified as Neotyphodium lolii from the investigation of ribosome RNA genes. The endophyte isolated Fukaura ecotype was inoculated to other endophyte free genotypes. Lolitrem B contents of inoculated plants ranged from low to extremely high levels. This was thought to an interaction between the endophyte strain and plant genotype. Growth characteristics of endophyte-infected (E+) plants were investigated under low P nutrient solution culture condition. In both perennial ryegrass and tall fescue, E+ plants showed larger secondary root system. Moreover, it was clarified that an endophyte has a beneficial influence for the mineral absorption. Mineral compositions in leaf blade were high in phosphorus and sulfur of E+ tall fescue plants and in phosphorus of E+ perennial ryegrass plants. In case E+ forage plants were practically used, it is required to check toxicity of alkaloid produced by endophyte to animals. Mice have been used to clarify the mechanism underlying the toxicity of endophyte alkaloids in livestock. When seeds powder densely infected with endophyte was fed, no mice showed any signs like stagger, but showed increased body temperature and decreased rotarod performance.
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