| Project/Area Number |
14206035
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | RIKEN |
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Principal Investigator |
AIDA Yoko RIKEN, Retrovirus Research Unit, Unit Reader, 分子ウイルス学研究ユニット, 研究ユニットリーダー (50182994)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHIMA Sin-Nosuke RIKEN, Retrovirus Research Unit, 分子ウイルス学研究ユニット, 協力研究員 (60342812)
田島 茂 理化学研究所, 分子ウイルス学研究ユニット, 基礎科学特別研究員 (60311346)
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥53,040,000 (Direct Cost: ¥40,800,000、Indirect Cost: ¥12,240,000)
Fiscal Year 2005: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2004: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2003: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2002: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
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| Keywords | bovine MHC (BoLA) region / BoLA-DRB3 / BoLA-DQA / mastitis / BLV-induced lymphoma / BLV epitope / diseases susceptibility / PCR-sequence based typing / BoLA-DQA1及び-DQA2 / 多型性 / PCR-Sequence Based Typing / BoLA-DOA及び-DQB / TAPトランスポーター / BoLAクラスI遺伝子 / BoLA-DOA及び-DOB / BLV誘発性の白血病発症 / ヒツジMHCクラスIIDRB1 / ウシMHO(BoLA)クラスIIDRB3 / PCR-Sequence-Based Typing(SBT)法 / 抗原認識部位(ARS) / ヒツジMHCクラスIIDRB1アリル / MHC拘束性CD4陽性細胞 / IFN-γ / IL-2 |
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| Research Abstract |
Genomic organization of bovine major histocompatibility complex (BoLA) : The draft sequence of entire bovine genome has been announced by Human Genome Sequencing Center, and the information has been available in NCBI genome project database (http://www.ncbi.nlm.nih.gov/). Here, we analyzed the comparative map of human MHC region on chromosome 6 (HSP6p) and BoLA region on chromosome 23 (BTA23), using the draft sequence of BoLA and complete sequence of human MHC region. Although the existence of two class II subregions in cattle gene map was known before (cf.class IIa and IIb), and is also confirmed in our comparative gene map based on the whole genome draft sequencing, some of the newly constructed gene correspondences show differences from the previous linkage map.
Relationship between BoLA class IIa region and development of mastitis and bovine leukemia virus (BLV)-induced lymphoma : BoLA class IIa region revealed to associate in various diseases and economic trait. The class IIa region contains BoLA-DRA, -DRB, -DQA and -DQB genes and shows a high degree of polymorphism. Recently, we developed new methods for sequence based typing (SBT) which allow identification of alleles of BoLA-DQA1 gene and BoLA-DRB3 gene of large numbers of animals with relative case, and showed that the DRB3^*0701-DQA1^*0101 and DRB3^*14012-DQA1^*10012 might protect against development of BLV-induced lymphoma. We now progress to identify BLV epitope which related to both alleles. Next, we performed the typing of DQA1 alleles of 188 cattle with mastitis and 95 normal Holstein cattle and calculated each allele frequency in two groups. The DQA1^*1201 were apparently low frequency in mastitis group as compared with a normal group (p=0.006663), suggesting that this allele might be positively related to resistance to mastitis. Collectively, the breeding by the marker selection using BoLA class II haplotype might probably contribute to the improvement of disease resistance traits in domestic cattle.
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