| Project/Area Number |
14206036
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKASHIMA Ikuo Hokkaido University, Graduate School of Veterinary Medicine, Professor, 大学院・獣医学研究科, 教授 (30002083)
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| Co-Investigator(Kenkyū-buntansha) |
KARIWA Hiroaki Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院・獣医学研究科, 助教授 (70224714)
MORITA Koichi Institute for Tropical Medicine, Nagasaki University, Professor, 熱帯医学研究所, 教授 (40182240)
TADANO Masayuki Medical School, Ryuku University, Associate Professor, 医学部, 助教授 (80179712)
TAKEGAMI Tsutomu Kanazawa Medical Schools, Institute for Medical Sciences, Professor, 総合医学研究所, 教授 (10113490)
ESHITA Yuki Ohita Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10082223)
水谷 哲也 国立感染症研究所, 主任研究員 (70281681)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000)
Fiscal Year 2004: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2003: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2002: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
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| Keywords | West Nile fever / Flavivirus infection / diagnosis / epidemiology |
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| Research Abstract |
In order to prevent the outbreaks of flavivirus infections, we developed the diagnostic tests, examined pathogenecity of the viruses and examined vector susceptibility of the Japanese mosquitoes. The results are summarized as follows.
1.Development of diagnostic tests.
1)As the genetic diagnostic tests of West Nile virus, RT-PCR RFLP method, a real time PCR method and RT-LAMP method were developed.
2)For tick-borne encephalitis, IgG-and IgM-ELISA using subviral particles were developed as serological diagnostic tests for human.
2.Studies on pathogenecity of flavivirus
1)Neuroinvasive virulence of West Nile virus New York strain was found to be associated with glysosylation of envelope protein.
2)Neuroinvasive virulence of tick-borne encephalitis virus was reduced due to one amino acid change of envelope protein which led to the reduced vircemia.
3.Japanese encephalitis
1)Japanese encephalitis virus from South-East Asian countries often invaded into Japan.
2)RNAi was found to be effective as antiviral agent to Japanese encephalitis viris.
4.Mosquito studies
1)Japanese indigenous mosquitoes of 4 species were found to be susceptible to West Nile virus.
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